mpidb The Microbial Protein Interaction Database krishnakumar-2007-1 Structural properties of periplasmic SodCI that correlate with virulence in Salmonella enterica serovar Typhimurium. salty Salmonella typhimurium molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Krishnakumar R., Kim B., Mollo E.A., Imlay J.A., Slauch J.M. J. Bacteriol. (0021-9193) 2007 Only protein-protein interactions imex curation krishnakumar-2007-2 Structural properties of periplasmic SodCI that correlate with virulence in Salmonella enterica serovar Typhimurium. in vitro In vitro enzymatic footprint enzymatic footprinting enzymatic footprint predetermined predetermined participant predetermined Krishnakumar R., Kim B., Mollo E.A., Imlay J.A., Slauch J.M. J. Bacteriol. (0021-9193) 2007 Only protein-protein interactions imex curation sodc1_salty Superoxide dismutase [Cu-Zn] 1 sodCI STM1044 sodC1 sodC protein protein salty Salmonella typhimurium MKYTILSLVAGALISCSAMAENTLTVKMNDALSSGTGENIGEITVSETPYGLLFTPHLNGLTPGIHGFHVHTNPSCMPGMKDGKEVPALMAGGHLDPEKTGKHLGPYNDKGHLGDLPGLVVNADGTATYPLLAPRLKSLSELKGHSLMIHKGGDNYSDKPAPLGGGGARFACGVIEK sodc1-sodc1 1331798 not-defined-1058513095 1331800 unspecified role unspecified role unspecified role unspecified role P0CW86:p.[Tyr107Glu;Tyr129Glu] P0CW86-87-109 mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 physical association physical association false false false figure 1 sodc1-sodc1-1 1331799 not-defined-1058513095 1331800 unspecified role unspecified role unspecified role unspecified role P0CW86:p.[Tyr107Glu;Tyr129Glu] P0CW86-87-109 mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false salty Salmonella typhimurium Stoichiometry: 2.0 direct interaction direct interaction false false false figure 2 Purified SodCI dier is resistant to proteinase K. Monomeric SodC from E. coli was inactivated. Protease-sensitive sites that are buried in the dimer become exposed in monomeric SodCI.