UniProt UniProt http://www.uniprot.org mimida-2007-1 Two alternatively spliced transcripts generated from OsMUS81, a rice homolog of yeast MUS81, are up-regulated by DNA-damaging treatments. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Mimida N., Kitamoto H., Osakabe K., Nakashima M., Ito Y., Heyer W.D., Toki S., Ichikawa H. chongk@ibcas.ac.cn Plant Cell Physiol. (0032-0781) 2007 Only protein-protein interactions imex curation mus81_yeast Crossover junction endonuclease MUS81 MUS81 SLX3 YDR386W D9509.6 MMS and UV-sensitive protein 81 protein protein yeast Saccharomyces cerevisiae Baker's yeast MELSSNLKDLYIEWLQELVDGLTPKQEQLKIAYEKAKRNLQNAEGSFYYPTDLKKVKGIGNTIIKRLDTKLRNYCKIHHISPVEAPSLTQTSSTRPPKRTTTALRSIVNSCENDKNEAPEEKGTKKRKTRKYIPKKRSGGYAILLSLLELNAIPRGVSKEQIIEVAGKYSDHCMTPNFSTKEFYGAWSSIAALKKHSLVLEEGRPKRYSLTEEGVELTKSLKTADGISFPKENEEPNEYSVTRNESSEFTANLTDLRGEYGKEEEPCDINNTSFMLDITFQDLSTPQRLQNNVFKNDRLNSQTNISSHKLEEVSDDQTVPDSALKAKSTIKRRRYNGVSYELWCSGDFEVFPIIDHREIKSQSDREFFSRAFERKGMKSEIRQLALGDIIWVAKNKNTGLQCVLNTIVERKRLDDLALSIRDNRFMEQKNRLEKSGCEHKYYLIEETMSGNIGNMNEALKTALWVILVYYKFSMIRTCNSDETVEKIHALHTVISHHYSQKDLIVIFPSDLKSKDDYKKVLLQFRREFERKGGIECCHNLECFQELMGKGDLKTVGELTIHVLMLVKGISLEKAVAIQEIFPTLNKILMAYKTCSSEEEAKLLMFNVLGDAPGAKKITKSLSEKIYDAFGKL F73FA3856C5F87F1 rad54_yeast DNA repair and recombination protein RAD54 G1821 YGL163C RAD54 protein protein yeast Saccharomyces cerevisiae Baker's yeast MARRRLPDRPPNGIGAGERPRLVPRPINVQDSVNRLTKPFRVPYKNTHIPPAAGRIATGSDNIVGGRSLRKRSATVCYSGLDINADEAEYNSQDISFSQLTKRRKDALSAQRLAKDPTRLSHIQYTLRRSFTVPIKGYVQRHSLPLTLGMKKKITPEPRPLHDPTDEFAIVLYDPSVDGEMIVHDTSMDNKEEESKKMIKSTQEKDNINKEKNSQEERPTQRIGRHPALMTNGVRNKPLRELLGDSENSAENKKKFASVPVVIDPKLAKILRPHQVEGVRFLYRCVTGLVMKDYLEAEAFNTSSEDPLKSDEKALTESQKTEQNNRGAYGCIMADEMGLGKTLQCIALMWTLLRQGPQGKRLIDKCIIVCPSSLVNNWANELIKWLGPNTLTPLAVDGKKSSMGGGNTTVSQAIHAWAQAQGRNIVKPVLIISYETLRRNVDQLKNCNVGLMLADEGHRLKNGDSLTFTALDSISCPRRVILSGTPIQNDLSEYFALLSFSNPGLLGSRAEFRKNFENPILRGRDADATDKEITKGEAQLQKLSTIVSKFIIRRTNDILAKYLPCKYEHVIFVNLKPLQNELYNKLIKSREVKKVVKGVGGSQPLRAIGILKKLCNHPNLLNFEDEFDDEDDLELPDDYNMPGSKARDVQTKYSAKFSILERFLHKIKTESDDKIVLISNYTQTLDLIEKMCRYKHYSAVRLDGTMSINKRQKLVDRFNDPEGQEFIFLLSSKAGGCGINLIGANRLILMDPDWNPAADQQALARVWRDGQKKDCFIYRFISTGTIEEKIFQRQSMKMSLSSCVVDAKEDVERLFSSDNLRQLFQKNENTICETHETYHCKRCNAQGKQLKRAPAMLYGDATTWNHLNHDALEKTNDHLLKNEHHYNDISFAFQYISH 2EC0A9B7E3D66292 rad54_orysj DNA repair and recombination protein RAD54 RAD54 OsJ_08484 P0486G03.23 LOC_Os02g52510 Os02g0762800 protein protein orysj Oryza sativa subsp. japonica (Rice) Rice MPSTSKCNRISRVADEEEEEEIVAVSSDADESESESEVGSGAEEEDDDYVGESSDSAGGSGSGSGDGDGDEEGGRSDIGDGEGEGGGRRVRSACRGVRANDRERKSQNVDALVRGNLVVRRQPLIPRILSVSDAAAIARKPFKPPCQNGYSENNEQLARRLSARKRFVPWGSVQPFAVTNILPQSPAVSSDDSVENEESLPPGIEPLILWQPEGRDKENSNFSAIKVDHLLVRYLRPHQREGVQFMFDCVSGLLNDDGISGCILADDMGLGKTLQSITLLYTLLCQGFDAKPMVKRAVVVTPTSLVSNWESEIIKWLKGRVQLLALCESTRADVLSGIESFLKPLSRLQVLIVSYETFRMHSSKFERPGSCDLLICDEAHRLKNDQTLTNKALAALPCKRRILLSGTPMQNDLEEFFSMVNFTNPGVLGDATYFRRYYEAPIICGREPTASAEEKNLGSERSAELSAKVNLFILRRTNALLSNHLPPKIVEVVCCKLTALQTALYNHFIHSKNVKRLISEGTKQSKVLAYITALKKLCNHPKLIYDTIKSNNSGGSGFDDCLRFFPPELFSGRSGSWTGGGGMWVELSGKMHVLARLLGHLRLKTDDRIVLVSNYTQTLDLFAQLCRERRYPYIRLDGATSINKRQKLVNQFNDPSRDEFVFLLSSKAGGCGLNLVGGNRLILFDPDWNPANDKQAAARVWRDGQKKRVYIYRFLSTGTIEEKVYQRQMSKEGLQKVIQQEQADGKMQGSSLSTEDLRDLFTFHEQIRSEIHENLKCNRCNKDGCMVLDGSKFDSAATEHEASNSGENSYIDIGGFGAISGCVQKMNSSNQQIGSPSEEDLGSWGHHSDPSTVPDTILQCSSGDEVSFVFTNQIDGKLVPVESMARAATHRTHEVTVNAEKEVGKINSSNVPGTERQSLLGKNLKMMGFNLKNSSMKFPTKSRRMLPNCLQGMNKTSTSSDHQQTKKLHVISDASDDDFV mus81_orysj Crossover junction endonuclease MUS81 MUS81 Protein MMS AND UV SENSITIVE 81 OsJ_04752 P0466H10.31 LOC_Os01g71960 Os01g0948100 protein protein orysj Oryza sativa subsp. japonica (Rice) Rice MAPEARQLKVHLRENEAVAQCVLEKWRSMEEKPGGLKENLAHTLYKSYRNVCAAKEPIRSLKDLYQIKGVGKWVIRQLKGSFPESSPDLSPPESNAAGEKGKKAGGSKRYVPQKNSAAYAILITLHRETINGKSHMKKQELIDATEASGLSRSAIGPDKSKAKPGAFASSQKDWYTGWSCMKTLTSKGLVAKSGNPAKYMITEEGKSTALECLSRSGLDDHAAPLVINSAPDTSNASHKLNNICMTSFVETSSGPSRAIGRPKTSIANPATKTSPEVTYLTSQESLNYNSDVRTAENCAEEIILSDSDSEELYTENYPLIGSEEFTERVAPPILNASNSGKTTTNYRFSDCSASISPRSSEGTFEMQSSSTMGIAEFNMLDNDTVCMDNSILAMPPRRSSKNFLEDYEVVLILDDRENFGGRSRKTVDNIHSQFRVPVEIKHLPVGDGIWIARDRKLHTEYVLDFIVERKNVADLCSSITDNRYKDQKLRLKKCGLRKLIYLVEGDPNPLDTSERIKTACFTTEILEGFDVQRTPGYAETVRTYGNLTHSITEYYSTHFSTGANTSQVCLTYDEFTKKCDDLKKITVSDVFALQLMQVPQVTEEAALAVIGLYPTLFSLAKAYSMLDGDTHAQEKMLKNKSTLINAGASRNIFKLVWAEG rad54-mus81 1295263 n/a 1295264 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1295265 unspecified role unspecified role bait bait c-term sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 1295272 1295270 physical association physical association false false false Fig. 3 osrad54-osmus81 1295263 n/a 1295266 unspecified role unspecified role bait bait n/a 1295267 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 3 osrad54-osmus81 1295263 n/a 1295266 unspecified role unspecified role bait bait c-term sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1295267 unspecified role unspecified role prey prey xpf-like region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 3