UniProt UniProt http://www.uniprot.org park-2006b-3 Frodo links Dishevelled to the p120-catenin/Kaiso pathway: distinct catenin subfamilies promote Wnt signals. xenla-embryo Xenopus laevis embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Park J.I., Ji H., Jun S., Gu D., Hikasa H., Li L., Sokol S.Y., McCrea P.D. pdmccrea@mdanderson.org Dev. Cell (1534-5807) 2006 Only protein-protein interactions imex curation park-2006b-5 Frodo links Dishevelled to the p120-catenin/Kaiso pathway: distinct catenin subfamilies promote Wnt signals. in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Park J.I., Ji H., Jun S., Gu D., Hikasa H., Li L., Sokol S.Y., McCrea P.D. pdmccrea@mdanderson.org Dev. Cell (1534-5807) 2006 Only protein-protein interactions imex curation park-2006b-1 Frodo links Dishevelled to the p120-catenin/Kaiso pathway: distinct catenin subfamilies promote Wnt signals. xenla-embryo Xenopus laevis embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Park J.I., Ji H., Jun S., Gu D., Hikasa H., Li L., Sokol S.Y., McCrea P.D. pdmccrea@mdanderson.org Dev. Cell (1534-5807) 2006 Only protein-protein interactions imex curation park-2006b-2 Frodo links Dishevelled to the p120-catenin/Kaiso pathway: distinct catenin subfamilies promote Wnt signals. xenla-embryo Xenopus laevis embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Park J.I., Ji H., Jun S., Gu D., Hikasa H., Li L., Sokol S.Y., McCrea P.D. pdmccrea@mdanderson.org Dev. Cell (1534-5807) 2006 Only protein-protein interactions imex curation park-2006b-4 Frodo links Dishevelled to the p120-catenin/Kaiso pathway: distinct catenin subfamilies promote Wnt signals. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Park J.I., Ji H., Jun S., Gu D., Hikasa H., Li L., Sokol S.Y., McCrea P.D. pdmccrea@mdanderson.org Xenopus gastrula cDNA Dev. Cell (1534-5807) 2006 Only protein-protein interactions imex curation park-2006b-6 Frodo links Dishevelled to the p120-catenin/Kaiso pathway: distinct catenin subfamilies promote Wnt signals. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 imaging technique imaging technique microscopy immunostaining immunostaining Park J.I., Ji H., Jun S., Gu D., Hikasa H., Li L., Sokol S.Y., McCrea P.D. pdmccrea@mdanderson.org Dev. Cell (1534-5807) 2006 Only protein-protein interactions imex curation dct1a_xenla Dapper 1-A Dapper1b dact1-a Functional regulator of dsh in ontogenesis protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MKPIPSPEPPGQLRLTPRRKDKGEAEWERHRTRERLEATLAGLAELDCLRHRQQLLVQNVLSPGTHGQDAAPTGDSPRSDEQKLLEENISLLKKQLNCLRKRDAGLLSQLHELDKQINDLKIDSEKTEETDSRPSSGFYELSDGTSGSLSNSSNSVFSECLSSCHSSTCFCNPLETSLNLTDGQAKSAEDFLEWLDYRESQHETGTVRCSFSAPHSNSVEVTADVHPKYQCDLVSKNGNDVYRYPSPLHAVAVQSPMFLISVMGNIKAEEPEEEIGPNDNDDCIVPELDHLKDEDSFLHQSSLCSLPLSSGKKMDGYILSIIHKKAHPVRTNKPRTSVNADPGKGILRHGSICVKQTVGVSQSNAVNLKNSKQTCLHSAGMISVDNSTYPSLKPCSKESLSEQLESKRMPSISTYPSCNVNELQSQNNSRNTVKSVCQGLARGSVAMTSNVQKENVTPNAPANLPNASSSVCNGSPRESTQNSALLPQEIKVVPPVKRVSLKNTLLSYHESSSFEERPPLDFKSEGSSSQSLDEGMLVNAHYIPAQQHGVKLHKNTKNVKIVKSSTLKHRADVQYVLENGSQTLKEKSKVVGKKCRFPEDLDTNKKVKKSTPRVKKIVHPHFEPAAVGRNPVAVRSGIKSHGHPKDVVLAKPKHKRSDYRRWKSSAEISYEEALRRARRRVQREMMGVCAQVPFSHASPYAYIASDSEYSAECESLFHSTVVDTSEDEQSNYTTNCFGDSESSLSEVEFVGESTTSSDTDESGGLIWSQFVHTLPMQATATAELQTTAKAFIKIKASHNLKKKILRFRSGSLKLMTTV ctnd1_xenla Catenin delta-1 ctnnd1 Xp120(ctn) p120 catenin protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MDEPESESPASILASVRAQEAQFELLSRALEEERRHVTAQLDRVWVTPQEPPLANGSLTRRQQDGLPFLYTPTRMEAHSVHADERYVIDDSSYKSCTLTDESRCSEIPIQTVVGYSQTLDRPYREAAGSGGAYTTVPRNYHFRGPGVDSTMPLISQSPHPGYSSLSRPNQRYRSVDPFRGPGYGPQPQVRGGGLGGSQSDLLSGRIYGSEDAYGLEDDRRSLGGFIDGPDYATTGRRGANGGDPRRRLRSYEDPLSDHFGSIPTSGSLSSLGPAPLTLAPNSGPWRHPELPEVLAMLSYTLDAVRLNAAAYLQHLSYRNEDVKREVCRLRGIPPLISLLEDPRAPIRLAACGALKNLSYGPARENKMAVKNCDGVPALARLLRRRGEGIEGRELAECVTGTLWNLSSLDSVKMELVDQALYTLTQEILVPHSGWQQDGGMQDRVEGKPRHVEWEPALVNTTGCLRNISSERSEARRKMRECEGLVDSVVHILRSEVSHGLVDSKLLENVVCLLRNISYHVHREIPHAEKYMESPQNASAETQNPSCFGVRRGKGKKASEEAVDTVDFPKQTMPAQGYDLLFQPEIVRLYISLVKSSHTPAVLEASAGAIQNLCAGNWVYGRCIRAAVRQEKGLSSLADHLTHESERVVRAICGALRNLCGDNRNRELIGKHALNSLVARLSSSSAQSSALSEDTNVCVINTIHEVISGNLEAAKRLRESQGIERLVLINKGGGRSEREIRAAGFCLQTIWGYKELRRPLEKDGWKKSDFQVSTAATSSVQGYDDSTLPLIDRNLKNEKKSVNADIPLNDFIADQFTHNGDSSNRLPTRSDELSELDPLKECSVSAGGSLNLGDAEDQRV kaiso_xenla Transcriptional regulator Kaiso zbtb33 kaiso Zinc finger and BTB domain-containing protein 33 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog METKKLITATDTQYSGILLNALNDQRIQGLYCDVTVIVEDRKFRAHRNILSACSTYFHQLFSVAGQVVELNFVKADIFAEILNYIYSSKIVRVRCDMLEELIKSGKLLGVPFIAELGIPLSQVKSISGAGGKDGGTDAPSNPDHKAPEPQKSSDSPLPCTVKIKADVKTEMPVITESFSLSSDDYKDKKASGSQDHNSEKEDDDDDVIFCSEIVSSKQAPAERKEAAQTQIPPDNEQVPEVKKVTPSSQVQLTQNSLPTNQQSSKNTSSTTQKFTPPVNANISKNPTPAANGFLSPTAQKQGTPNAVQNQHSQNITSGNALPQQKPVVNFSSIKPQQISAIKPKTEVIIHGNGLSPPSSSVIPLGQQPVTPKHISFDGVQKKQVVTFTQGSPSKPGEFKIKIADVVSGSSLDSFKDSEPRRIIDGKKIITLDTASEIEGLSTGCKVYANIGEDTYDIVIPIKEDPEEGEAKLDLDGLPNRKRMKLKHDDHYELIVDGRVYYICIVCKRSYVCLTSLRRHFNVHSWEKKYPCRYCERVFPLAEYRTKHEIHHTGERRYQCLTCGSSFINYQVMASHIRSVHSLDPSGDSKLYRLNPCKTLQIRQYAYVNNSTNGTVINDGAINVPVITDGGINVPVINDGGIVYDIDPDEPQQPASEGNHANSATKPVNWDNIFIQQSNQNMFKLNTSEGGTEFEFVIPESY dvl2_xenla Segment polarity protein dishevelled homolog DVL-2 dvl2 dsh DSH homolog 2 Xdsh protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MAETKVIYHLDEEETPYLVKVPVPATDIRLRDFKAALGRGHAKYFFKAMDQDFGVVKEEISDDNAKLPCFNDRVVSWLASSEGSQPDSAPPAPATEVRPEPPPPVPPPIPPPPAERTSGIGDSRPPSFHPNVSGSTEQLDQDNESVISMRRDRVRRRESSEQAGVGRGVNGRTERHLSGYESSSTLLTSEIETSICDSEEDDTMSRFSSSTEQSSASRLLKRHRRRRKQRPPRLERTSSFSSVTDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDINFENMSNDDAVRVLRDIVHKPGPIVLTVAKCWDPSPQGYFTLPRNEPIHPIDPAAWVSHSAALSGSFPVYPGSASMSSMTSSTSVTETELSHALPPVSLFSLSVHTDLASVVKVMASPESGLEVRDRMWLKITIPNAFLGSDVVDWLYHHVEGFQDRREARKFASNLLKAGFIRHTVNKITFSEQCYYIFGDLTGCENYMTNLSLNDNDGSSGASDQDTLAPLPLPGASPWPLLPTFSYQYQAPHPYSTQPPAYHELSSYSYGMGSAGSQHSEGSRSSGSNRSDGGRGMQKDDRSGVAGVGGGDSKSGSGSESEYSTRSSIRRVGGGEAGPPSERSTSSRLPPHHPPSVHSYAAPGVPLSYNPMMLMMMPPPPLPPPGVCPPNSSVPPGAPPLVRDLASVPPELTATRQSFHMAMGNPSEFFVDVM dact1_a-ctnnd1-2 1311027 n/a 1311033 unspecified role unspecified role bait bait nucl microinjection nucleic acid microinjection nucl microinjection region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false n/a 1311034 unspecified role unspecified role prey prey nucl microinjection nucleic acid microinjection nucl microinjection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1C and E zbtb33-ctnnd1-3 1311027 n/a 1311035 unspecified role unspecified role bait bait nucl microinjection nucleic acid microinjection nucl microinjection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1311034 unspecified role unspecified role prey prey nucl microinjection nucleic acid microinjection nucl microinjection region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 5D dact1-a/frodo (UniProt Ac Q8JJ48) ctnnd1-dvl2 1311027 n/a 1311036 unspecified role unspecified role prey prey nucl microinjection nucleic acid microinjection nucl microinjection region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1311034 unspecified role unspecified role bait bait nucl microinjection nucleic acid microinjection nucl microinjection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 6H dvl2-ctnnd1 1311027 n/a 1311036 unspecified role unspecified role bait bait nucl microinjection nucleic acid microinjection nucl microinjection region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1311034 unspecified role unspecified role prey prey nucl microinjection nucleic acid microinjection nucl microinjection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 6H ctnnd1-zbtb33-5 1311028 n/a 1311035 unspecified role unspecified role unspecified role unspecified role in vitro translated in vitro translated protein in vitro translated region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes n/a 1311034 unspecified role unspecified role bait bait in vitro translated in vitro translated protein in vitro translated region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes physical association physical association false false false 6I ctnnd1-dact1_a 1311028 n/a 1311033 unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes n/a 1311034 unspecified role unspecified role bait bait in vitro translated in vitro translated protein in vitro translated region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes physical association physical association false false false 6I dact1_a-ctnnd1 1311029 n/a 1311034 unspecified role unspecified role prey prey n/a 1311033 unspecified role unspecified role bait bait physical association physical association false false false 1A dact1_a-ctnnd1-1 1311030 n/a 1311033 unspecified role unspecified role bait bait nucl microinjection nucleic acid microinjection nucl microinjection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1311034 unspecified role unspecified role prey prey physical association physical association false false false 1B dact1_a-ctnnd1-3 1311031 n/a 1311034 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined c-term range C-terminal range c-term range false n/a 1311033 unspecified role unspecified role bait bait physical association physical association false false false no figure ctnnd1-dact1_a-1 1311032 n/a 1311033 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1311034 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection colocalization colocalization false false false S1