mbinfo MBInfo http://www.mechanobio.info/ http://www.mechanobio.info/ nyman_huttunen-2006- alpha-Actinin-dependent cytoskeletal anchorage is important for ICAM-5-mediated neuritic outgrowth. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Nyman-Huttunen H., Tian L., Ning L., Gahmberg C.G. Interaction detection: Pull-downs were carried out using either a glutathione-sepharose column (Fig 1B) or a thiopropyl-sepharose column (Fig 1c). Experiments involving endogenous alpha-actinin could not be curated since the specific isoform of alpha-actinin involved in the interaction could not be identified. carl.gahmberg@helsinki.fi J. Cell. Sci. (0021-9533) 2006 Only protein-protein interactions imex curation nyman_huttunen-2006- alpha-Actinin-dependent cytoskeletal anchorage is important for ICAM-5-mediated neuritic outgrowth. in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Nyman-Huttunen H., Tian L., Ning L., Gahmberg C.G. Interaction detection: Pull-downs were carried out using either a glutathione-sepharose column (Fig 1B) or a thiopropyl-sepharose column (Fig 1c). Experiments involving endogenous alpha-actinin could not be curated since the specific isoform of alpha-actinin involved in the interaction could not be identified. carl.gahmberg@helsinki.fi J. Cell. Sci. (0021-9533) 2006 Only protein-protein interactions imex curation icam5_human Intercellular adhesion molecule 5 ICAM5 TLCN TLN Telencephalin protein protein human Homo sapiens Human MPGPSPGLRRALLGLWAALGLGLFGLSAVSQEPFWADLQPRVAFVERGGSLWLNCSTNCPRPERGGLETSLRRNGTQRGLRWLARQLVDIREPETQPVCFFRCARRTLQARGLIRTFQRPDRVELMPLPPWQPVGENFTLSCRVPGAGPRASLTLTLLRGAQELIRRSFAGEPPRARGAVLTATVLARREDHGANFSCRAELDLRPHGLGLFENSSAPRELRTFSLSPDAPRLAAPRLLEVGSERPVSCTLDGLFPASEARVYLALGDQNLSPDVTLEGDAFVATATATASAEQEGARQLVCNVTLGGENRETRENVTIYSFPAPLLTLSEPSVSEGQMVTVTCAAGAQALVTLEGVPAAVPGQPAQLQLNATENDDRRSFFCDATLDVDGETLIKNRSAELRVLYAPRLDDSDCPRSWTWPEGPEQTLRCEARGNPEPSVHCARSDGGAVLALGLLGPVTRALSGTYRCKAANDQGEAVKDVTLTVEYAPALDSVGCPERITWLEGTEASLSCVAHGVPPPDVICVRSGELGAVIEGLLRVAREHAGTYRCEATNPRGSAAKNVAVTVEYGPRFEEPSCPSNWTWVEGSGRLFSCEVDGKPQPSVKCVGSGGATEGVLLPLAPPDPSPRAPRIPRVLAPGIYVCNATNRHGSVAKTVVVSAESPPEMDESTCPSHQTWLEGAEASALACAARGRPSPGVRCSREGIPWPEQQRVSREDAGTYHCVATNAHGTDSRTVTVGVEYRPVVAELAASPPGGVRPGGNFTLTCRAEAWPPAQISWRAPPGALNIGLSSNNSTLSVAGAMGSHGGEYECAATNAHGRHARRITVRVAGPWLWVAVGGAAGGAALLAAGAGLAFYVQSTACKKGEYNVQEAESSGEAVCLNGAGGGAGGAAGAEGGPEAAGGAAESPAEGEVFAIQLTSA actn1_chick Alpha-actinin-1 ACTN1 Alpha-actinin cytoskeletal isoform F-actin cross-linking protein Non-muscle alpha-actinin-1 protein protein chick Gallus gallus (Chicken) Chicken MDHHYDPQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRAPENTMQAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDINNAWGGLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHESWTDGKEAMLQQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERQAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWEHVRQLVPRRDQALMEEHARQQQNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLNHLRQYEKSIVNYKPKIDQLEGDHQQIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDAQGEAEFARIMSIVDPNRMGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITVDELRRELPPDQAEYCIARMAPYNGRDAVPGALDYMSFSTALYGESDL icam5-actn1-2 1331146 n/a 1331148 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false The cytoplasmic domain sequence [852-912] given by the author corresponds to sequence [861-916] of the uniprot entry for the human ICAM-5. ecoli Escherichia coli (strain K12) n/a 1331149 unspecified role unspecified role prey prey chick-gizzard Chicken gizzard gizzard gizzard physical association physical association false false false Fig 1B(b). icam5-actn1-4 1331146 n/a 1331149 unspecified role unspecified role prey prey chick-gizzard Chicken gizzard gizzard gizzard n/a 1331148 unspecified role unspecified role bait bait Q9UMF0:p.Lys866Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false The amino acid K857 specified by the author corresponds to the amino acid K866 of the uniprot entry for human ICAM-5. region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false The binding region sequence [857-861] specified by the author corresponds to the sequence [866-870] of the uniprot entry for human ICAM-5. Q9UMF0:p.Lys867Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false The amino acid K858 specified by the author corresponds to the amino acid K867 of the uniprot entry for human ICAM-5. Q9UMF0:p.[Lys866Ala;Lys867Ala] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false The amino acids K857 and K858 specified by the author corresponds to the amino acids K866 and K867 of the uniprot entry for human ICAM-5. c-terminal region cys L-cysteine residue (2R)-2-amino-3-sulfanylpropanoic acid (R)-cysteine 2-amino-3-mercaptopropanoic acid 2-amino-3-mercaptopropionic acid 2-azanyl-3-sulfanylpropanoic acid 3-mercapto-L-alanine L-(+)-cysteine L-cysteine alpha-amino-beta-mercaptopropanoic acid alpha-amino-beta-mercaptopropionic acid alpha-amino-beta-thiolpropionic acid beta-mercaptoalanine half-cystine thioserine Cys Cysteine (C, Cys) c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q9UMF0:p.Lys866Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false The amino acid K857 specified by the author corresponds to the amino acid K866 of the uniprot entry for human ICAM-5. Q9UMF0:p.Lys867Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false The amino acid K858 specified by the author corresponds to the amino acid K867 of the uniprot entry for human ICAM-5. chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false Fig 1C(a). actn1-icam5 1331147 n/a 1331148 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false The binding region sequence [857-861] specified by the author corresponds to the sequence [866-870] of the uniprot entry for human ICAM-5. region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1331149 unspecified role unspecified role neutral component neutral component chick-gizzard Chicken gizzard gizzard gizzard physical association physical association false false false 1331147 Fig 1E.