IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ sato-2005a-1 Caenorhabditis elegans RME-6 is a novel regulator of RAB-5 at the clathrin-coated pit. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Sato M., Sato K., Fonarev P., Huang C.J., Liou W., Grant B.D. grant@biology.rutgers.edu Nat. Cell Biol. (1465-7392) 2005 Only protein-protein interactions imex curation sato-2005a-2 Caenorhabditis elegans RME-6 is a novel regulator of RAB-5 at the clathrin-coated pit. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Sato M., Sato K., Fonarev P., Huang C.J., Liou W., Grant B.D. grant@biology.rutgers.edu Nat. Cell Biol. (1465-7392) 2005 Only protein-protein interactions imex curation sato-2005a-3 Caenorhabditis elegans RME-6 is a novel regulator of RAB-5 at the clathrin-coated pit. caeel Caenorhabditis elegans anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Sato M., Sato K., Fonarev P., Huang C.J., Liou W., Grant B.D. The molecule "worm alpha-adaptin/apt-4 (T20G5.1)" has been entered as: T20B5.1, as the ORF T20G5.1 corresponds to chc-1 rather than Apt-4. grant@biology.rutgers.edu Nat. Cell Biol. (1465-7392) 2005 Only protein-protein interactions imex curation sato-2005a-4 Caenorhabditis elegans RME-6 is a novel regulator of RAB-5 at the clathrin-coated pit. caeel Caenorhabditis elegans fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Sato M., Sato K., Fonarev P., Huang C.J., Liou W., Grant B.D. The molecule "worm alpha-adaptin/apt-4 (T20G5.1)" has been entered as: T20B5.1, as the ORF T20G5.1 corresponds to chc-1 rather than Apt-4. grant@biology.rutgers.edu Nat. Cell Biol. (1465-7392) 2005 Only protein-protein interactions imex curation sato-2005a-5 Caenorhabditis elegans RME-6 is a novel regulator of RAB-5 at the clathrin-coated pit. caeel Caenorhabditis elegans anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Sato M., Sato K., Fonarev P., Huang C.J., Liou W., Grant B.D. The molecule "worm alpha-adaptin/apt-4 (T20G5.1)" has been entered as: T20B5.1, as the ORF T20G5.1 corresponds to chc-1 rather than Apt-4. grant@biology.rutgers.edu Nat. Cell Biol. (1465-7392) 2005 Only protein-protein interactions imex curation rme6_caeel Receptor-mediated endocytosis protein 6 rme-6 F49E7.1 protein protein caeel Caenorhabditis elegans MADIIERIRSQDEYREFYELATKIRNQKLLTDAELHSLNRHQQEVSDNEIKLLTEAWRSSYWNNVGRQLKNQALEPTIACRLLKEISETEALPAYKHVGHHFSTLDQLLTILYNEPQSVAELLNSIDRNDITSNDSTIQVLFHLVYSCALYPEDELKIAQVVCNLLKLQLYRSGSEMRTILRKETSISTRFYKHFVELIHPTMVYLTKALRKGVLDVIQLGSFWLDIDAKQSTARFLRDNKQNEDRLPEYRALVVSKLVDFVDTFLENISKALPMLPPNLNWIIRDIFCSLYEVVDDVSAIELSHACKDMIVSNLICPAIISPQKFGVVDNDVRVGTIVNHNLVQIAMIIQMISLREFESPPEEYKEFLSQCRNTHLISEMMDALLVEKLAPDVEITSLIANGTAESDMATKSSFVGTVADVNVLIKIIRNAPSTSDAKLSRILSVCDKLPATISSTTQKSHFDNLEASPKISTLRNIHRKVQHSFKRTGDSFNDINELGAKQGEALAFGKENFDLFYLEYSPKNYKDVAEADRLKKKAEERRQLADLEKLLEPTPAPKETPVENNLIDFSSGSAETNSEHLSDSTSVSPEPLTSTEELQIGDQGVGGEQRGKLAKLKSLSDRMKKGITQSNTLSDIREHLRRSGSFVKPPPSGIPTSSSEQNLPDVATGPRDDILAKYASISSIKEQKPALGNLIDGTASASSTPRKEPLEPYYSSENLVGCRAFQDTLRKMITVLGNISYLPRIGYRNETKQNEWDKKVLLGRFLDGVLVETEHRREYGLAAQLREVKRCIELFEHAGVEILMDHLKLNEAEQDKIVNGMREERASLMRKSNDISSLEQRVLLNRRLTEQILVDFLMRTFLETGFNNKHTVGKTQEVTAVLKFYDEFKYLRAQDERAEFLKNLLTFLRDRLMQNVDWNFATDTMMSRAMTTIERYVIFAVYDNAFYPNRDADHHRDKLLRGTIAKVSDVVTPVNDFLKIPEHLHGEAPWPSAQAELSMLDIYVTAQDKLNCVVRCCDVINNLVALSSKNAVASADDLTPVLVFVIIKANPRALLSNVQFVETFAGDRIESGRDAYYWVNFKSAVEYIKTIL p91857_caeel rab-5 CELE_F26H9.6 F26H9.6 protein protein caeel Caenorhabditis elegans MAARNAGTARPGGPNRTCQFKLVLLGESAVGKSSLVLRFVKGQFHEYQESTIGAAFLTQTVCLDDATIKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNQESFQKAKNWVKELQRQASPNIVMALAGNKADVANKRTVEYEEANAYAEDNALLFMETSAKTSMNVNDIFMAIAKKLPIGPAQGEPTGTVDMNQPQQQQKGSCCK q8i100_caeel rabx-5 CELE_Y39A1A.5 Y39A1A.5 protein protein caeel Caenorhabditis elegans MSDKHGEEDKKAKMHLKANDLLCVNGCGFYGTPQWENRCSKCWRAHQNEMKKCQDFAKNRSLLSFDQFQERRKSTTESKSRGIKNLFKTSPIPEGGTSSPTSTSPASTPTRRRELSPDSLEARQQFTDFLVANLSTGMAQEIARSVKNAVNKISEMRMSSDDMSELVMSYYQYLGERIGGHSLFDSPDCKVKVEDVMDQVEKYISTFCYSIFFCANHEEEVADMSLQDRIRSLHWVTAGFLETKMVFKKQTVRDKIDEAISELIEINAKRSAFEKLDCLTKSCKAIFEALKESEASTSADEFLPTLIYVLFRGNPPLIQSNVKFISRFAIPARLMSGEAAYFFTNLSCALEFARNMNHESLQMEKSEFEAYTSGHLAPPLSVINSACNQAIYVLEGTIETITNVAKKAGSLAKNLTNMHGKSDDDLEKMLAVIKETVDFYPTDEYMNMKQSIFAEEKETADILVSLSRQSSESGRGTLTDTQPSTPQPDTSLESPLIAEIQAINLTSSGNQEHVEEA q19088_caeel rabn-5 F01F1.4 CELE_F01F1.4 protein protein caeel Caenorhabditis elegans MTEPSASEEVSAGVNAEEPPIPSKKISQVSPNPTLQCEMCQNYEVNLTALQENERKLKEEVKAAMELNERYQLDLSNERDYRKELEKKMNELSTNCLNQVNEAAKANAKSEQRLDELSAKHDKSIEVFTDQLNLARDKLQQADDDFVTLGKKYKKLLGSTRKSAQELRSEKIDMPADLDQLQFICLKNREELIETRAAKEHTEQQLQDEITVLRAQILEERAHREDQEVTFTAEISRLQSELGKINTRMVVASETIADQDSNIRQIRDLQTTVGELETQVQQVQNERAAVEQTAQNYKQRCTSLQQELDTSEVVQRDFVKLSQSLQIQLEKIRQSEQEVRWQWDDDVDQCSNCDTSFARVKVKPHCLHCGRIFCMNCLKDTVPSGPNHRPANVCKVCHTLLNRDTKPFFATQDK q22601_caeel AP-2 complex subunit alpha T20B5.1 apa-2 CELE_T20B5.1 protein protein caeel Caenorhabditis elegans MPAVKGDGMRGLAVFISDIRNCKSKEAELKRINKELANIRSKFKGDKTLDGYQKKKYVCKLLFIFLLGNDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLIEQQSDLMKLIVQGIRNDLTSRNPVHVNLALQCISNMGSREMVEAFCTDLPKLLVSGETIDFVKQSAALCILKLFRNSPDSFQPGDYASRIVHLLNDSHMGVVTSAASLIEALSKKWPEEYKGAVPLAISRLSRIVTATYTDLQDYTYYFVPAPWLCVKLLRLLQNYPPPDDPSNKARLLECLEGILNKAQDAPKSKKVQHSNAKNAVLFEAIALIIHMDSEPQLLVRACNQLGTFLSHRETNLRYLALESMCLLATSEFSHDAVKKHQDTIINSLKTERDVSVRQRAVDLLYAMCDRSNANQIVAEMLAYLETADYSIREEMVLKVAILAEKYATDYTWYVDVILKLIRIAGDYVSEEVWYRVIQIVVNREDVQGYAAKTVFEALQRPACHENMVKVGGYILGEFGNFIAGDERSTAKIQFELLHSKFHLCSITTRCLLLTTYIKFCNLFPEIKPLVQQVFQTDHNLRNPDAELQQRSIEYLQMTKLASNDVLATILEVMPAFAEKESSLLAKLKKSKPQLEEIEREEKEKRSKPTAVMSEGSTSLVDFDSTNDTTASLADVFANNSGTGLGAQGDEVEIANKDDYLKFVTKSNAILWEDDYIQIGCKLETRNNLGRLGMFYGNKTSQPFNKFTPIITCPGALAVQLQAQAKPVEPVVAAGTQVQQLINFVCVQEFQKMPIMNIKFTFTDRAGAVQNFDKNFYLPLFISKFFEPTNMTSEQFFTRWKSLGAASQEAQKIFNALSPIEHATIESRLKGFGANLLTDVDPNPDNYVCAGIIHTQTQQIGTLIRLEPNKQAKMYRLTIRSSKDTVVQTLVDLLGNQF rme6-rab5 1252632 n/a 1252637 unspecified role unspecified role bait bait n/a 1252638 unspecified role unspecified role prey prey P91857:p.Ser33Asn mutation mutation certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 2 RME-6 specifically interacted with RAB-5(S33N). rabx5-rab5 1252632 n/a 1252639 unspecified role unspecified role bait bait n/a 1252638 unspecified role unspecified role prey prey physical association physical association false false false Supplementary Fig 2 rabx5-rabn 1252632 n/a 1252639 unspecified role unspecified role bait bait n/a 1252640 unspecified role unspecified role prey prey physical association physical association false false false Supplementary Fig 2 rab5-rme6-2 1252633 n/a 1252638 unspecified role unspecified role bait bait P91857:p.Ser33Asn mutation mutation certain certain sequence position certain certain certain sequence position certain false n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1252637 unspecified role unspecified role prey prey n_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig 2 RME-6 specifically interacted with RAB-5(S33N). rme_6-g5ed80 1252634 n/a 1252641 unspecified role unspecified role prey prey n/a 1252637 unspecified role unspecified role bait bait n_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig 8e apa_2-rme_6-1 1252635 n/a 1252637 unspecified role unspecified role neutral component neutral component n_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1252641 unspecified role unspecified role neutral component neutral component n_terminus rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false colocalization colocalization false false false Fig 7b apa_2-rme_6-2 1252636 n/a 1252641 unspecified role unspecified role bait bait n/a 1252637 unspecified role unspecified role prey prey n_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig 8f