IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ duzhyy-2005-4 A secretory-type protein, containing a pentraxin domain, interacts with an A-type K+ channel. crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Duzhyy D., Harvey M., Sokolowski B. bsokolow@health.usf.edu J. Biol. Chem. (0021-9258) 2005 Only protein-protein interactions imex curation duzhyy-2005-1 A secretory-type protein, containing a pentraxin domain, interacts with an A-type K+ channel. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Duzhyy D., Harvey M., Sokolowski B. bsokolow@health.usf.edu Sensory epithelium of embryonic chick cochlea cDNA J. Biol. Chem. (0021-9258) 2005 Only protein-protein interactions imex curation duzhyy-2005-2 A secretory-type protein, containing a pentraxin domain, interacts with an A-type K+ channel. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Duzhyy D., Harvey M., Sokolowski B. bsokolow@health.usf.edu J. Biol. Chem. (0021-9258) 2005 Only protein-protein interactions imex curation duzhyy-2005-3 A secretory-type protein, containing a pentraxin domain, interacts with an A-type K+ channel. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Duzhyy D., Harvey M., Sokolowski B. bsokolow@health.usf.edu J. Biol. Chem. (0021-9258) 2005 Only protein-protein interactions imex curation duzhyy-2005-5 A secretory-type protein, containing a pentraxin domain, interacts with an A-type K+ channel. crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. fluorescence imaging fluorescence microscopy fluorescence imaging anti tag immunost anti tag immunostaining anti tag immunost Duzhyy D., Harvey M., Sokolowski B. bsokolow@health.usf.edu J. Biol. Chem. (0021-9258) 2005 Only protein-protein interactions imex curation duzhyy-2005-6 A secretory-type protein, containing a pentraxin domain, interacts with an A-type K+ channel. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 fluorescence imaging fluorescence microscopy fluorescence imaging anti tag immunost anti tag immunostaining anti tag immunost Duzhyy D., Harvey M., Sokolowski B. bsokolow@health.usf.edu J. Biol. Chem. (0021-9258) 2005 Only protein-protein interactions imex curation q5umh8_chick protein protein chick Gallus gallus (Chicken) Chicken MLPGGVLSLLMLFCVFRASASVLDEGDDYDLMYVNLDNEIEGPVLGTEESASCDCQREHTEWDKLFIMLENSQMKENMMLQALEDGLKADVQAIRAELNQLTAGLAGTFSSTVQQLTSHALAQLEQALQRNGDQPEDAERLCESQQGKVLEQVLQLSHNVSARLGQLESSWLRWAEREAQETAFQLQQDKLGHSREDNLLLNTLWKELQQTRSELKASQKWASQHLLPAGYETAILFPMRSRKIFGSIHPTAAMTLSSFTVCVWVKATEALDKTIIFSYGTKLNPYEIQLYLSRQSAVLAVGSAQHKLAARDVVVPGKWIHLCGTWSSDNGTASLWVDGELTATAVGIADTHIVPDGGILQVGQEKNGCCVGGGFDEALAFSGKLTGLNLWDRVLSTEEIAAQGGEDACSSRGNIVGWGVTEVLLYGGAQYVS q8uw33_chick Potassium voltage-gated channel subfamily D member 2 kv4.2 Voltage-gated potassium channel subunit Kv4.2 protein protein chick Gallus gallus (Chicken) Chicken MAAGVPAWLPFARAAAIGWMPVATGPMPAAPRQERKRSQDSLIVLNVSGIQFQTWLDTLERYPDTLLGSSERDFFYHPETQQYFFDRDPDIFRHILNFYRTGKLHYPRQECISAYDEELAFFGIIPEIIGDCCYEEYKDRRRENAERLQDDADQDHTAESSLPSMTARQRMWRAFENPHTSTLALVFYYVTGFFIAVSVIANVVETVPCGVSPGRIKELPCGERYAVAFFCLDTACVMIFTVEYLLRLLAAPSRYKFVRSVMSIIDVVAIMPYYIGLVMTDNEDVSGAFVTLRVFRVFRIFKFSRHSQGLRILGYTLKSCASELGFLLFSLTMAIIIFATVMYYAEKGSSASKFTSIPAAFWYTIVTMTTLGYGDMVPKTIAGKIFGSICSLSGVLVIALPVPVIVSNFSRIYHQNQRADKRRAQKKARLARIRAAKSGSANAYMQSKRNGLLSNQLQQSSSEEEQAFVSKSGSSFETQHHHLLHCLEKTTNHEFVDEQLYEESCMEVSTVNRPPSHSPSLSSQQGVTGTCCSRRHKKTYRIPNTALTGSRHGSVQELSTIQIRCVERTPLSNSRSSLNAKVEECVKLNCEQPYVTTAIISIPTPPVTTPEGDDRPESPEYSGGNIVRVSAL q5umh8-q8uw33 1267625 n/a 1267631 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged certain certain sequence position certain certain certain sequence position certain false n/a 1267632 unspecified role unspecified role prey prey c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 5A q8uw33-q5umh8-3 1267625 n/a 1267632 unspecified role unspecified role bait bait c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1267631 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 5B q8uw33-q5umh8 1267626 n/a 1267632 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1267631 unspecified role unspecified role prey prey physical association physical association false false false Two clones appear to code for a PPTX isoform with a deletion of aa 130–155. Fig. 1 q8uw33-q5umh8-1 1267627 n/a 1267631 unspecified role unspecified role prey prey n/a 1267632 unspecified role unspecified role bait bait physical association physical association false false false Fig. 3H q8uw33-q5umh8-2 1267628 n/a 1267632 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1267631 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false 1267654 1267652 physical association physical association false false false Fig. 4 q5umh8-q8uw33-2 1267629 n/a 1267632 unspecified role unspecified role neutral component neutral component c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1267631 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false colocalization colocalization false false false Fig. 6C q5umh8-q8uw33-3 1267630 n/a 1267631 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged certain certain sequence position certain certain certain sequence position certain false n/a 1267632 unspecified role unspecified role neutral component neutral component c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false colocalization colocalization false false false Two clones appear to code for a PPTX isoform with a deletion of aa 130–155. Fig. 6F