mbinfo MBInfo http://www.mechanobio.info/ http://www.mechanobio.info/ eden-2002-1 Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck. bovin-brain Bos taurus brain brain Brain [cerebrum] chromatography chromatography technology column chromatography chromatography de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Eden S., Rohatgi R., Podtelejnikov A.V., Mann M., Kirschner M.W. The WAVE1 protein from bovine brain extracts cofractionated as a complex which was resolved by SDS PAGE and identified by Mass spectrometry. The source of the Wave1 complex proteins (all endogenous) identified by the cells (bovine brain) used. marc@hms.harvard.edu Nature (0028-0836) 2002 Only protein-protein interactions imex curation eden-2002-2 Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck. bovin-brain Bos taurus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Eden S., Rohatgi R., Podtelejnikov A.V., Mann M., Kirschner M.W. The WAVE1 protein from bovine brain extracts cofractionated as a complex which was resolved by SDS PAGE and identified by Mass spectrometry. The source of the Wave1 complex proteins (all endogenous) identified by the cells (bovine brain) used. marc@hms.harvard.edu Nature (0028-0836) 2002 Only protein-protein interactions imex curation eden-2002-3 Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Eden S., Rohatgi R., Podtelejnikov A.V., Mann M., Kirschner M.W. The WAVE1 protein from bovine brain extracts cofractionated as a complex which was resolved by SDS PAGE and identified by Mass spectrometry. The source of the Wave1 complex proteins (all endogenous) identified by the cells (bovine brain) used. marc@hms.harvard.edu Nature (0028-0836) 2002 Only protein-protein interactions imex curation a8yxx8_bovin BRK1 C22H3ORF10 protein protein bovin Bos taurus (Bovine) Bovine MAGQEDPVQREIHQDWANREYIEVITSSIKKIADFLNSFDMSCRSRLATLNEKLTALERRIEYIEARVTKGETLT f1mpz9_bovin NCKAP1 protein protein bovin Bos taurus (Bovine) Bovine MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKQGQVWKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLSLSDHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA f1mx60_bovin Cytoplasmic FMR1-interacting protein CYFIP2 protein protein bovin Bos taurus (Bovine) Bovine MTTHVTLEDALSNVDLLEELPLPDQQPCIEPPPSSIMYQANFDTNFEDRNAFVTGIARYIEQATVHSSMNEMLEEGHEYAVMLYTWRSCSRAIPQVKCNEQPNRVEIYEKTVEVLEPEVTKLMKFMYFQRKAIERFCSEVKRLCHAERRKDFVSEAYLLTLGKFINMFAVLDELKNMKCSVKNDHSAYKRAAQFLRKMADPQSIQESQNLSMFLANHNRITQCLHQQLEVIPGYEELLADIVNICVDYYENKMYLTPSEKHMLLKVMGFGLYLMDGNVSNIYKLDAKKRINLSKIDKFFKQLQVVPLFGDMQIELARYIKTSAHYEENKSKWTCTQSSISPQYNICEQMVQIRDDHIRFISELARYSNSEVVTGSGLDSQKSDEEYRELFDLALRGLQLLSKWSAHVMEVYSWKLVHPTDKFCNKDCPGTAEEYERATRYNYTSEEKFAFVEVIAMIKGLQVLMGRMESVFNQAIRNTIYAALQDFAQVTLREPLRQAVRKKKNVLISVLQAIRKTICDWEGGREPPNDPCLKGEKDPKGGFDIKVPRRAVGPSSTQLYMVRTMLESLIADKSGSKKTLRSSLDGPIVLAIEDFHKQSFFFTHLLNISEALQQCCDLSQLWFREFFLELTMGRRIQFPIEMSMPWILTDHILETKEPSMMEYVLYPLDLYNDSAYYALTKFKKQFLYDEIEAEVNLCFDQFVYKLADQIFAYYKAMAGSVLLDKRFRAECKNYGVIIPYPPSNRYETLLKQRHVQLLGRSIDLNRLITQRISAAMYKSLDQAISRFESEDLTSIVELEWLLEINRLTHRLLCKHMTLDSFDAMFREANHNVSAPYGRITLHVFWELNFDFLPNYCYNGSTNRFVRTAIPFTQEPQRDKPANVQPYYLYGSKPLNIAYSHIYSSYRNFVGPPHFKTICRLLGYQGIAVVMEELLKIVKSLLQGTILQYVKTLIEVMPKICRLPRHEYGSPGILEFFHHQLKDIIEYAELKTDVFQSLREVGNAILFCLLIEQALSQEEVCDLLHAAPFQNILPRVYIKEGERLEVRMKRLEAKYAPLHLVPLIERLGTPQQIAIAREGDLLTKERLCCGLSMFEVILTRIRSYLQDPVWRGPPPTNGVMHVDECVEFHRLWSAMQFVYCIPVGTNEFTAEQCFGDGLNWAGCSIIVLLGQQRRFDLFDFCYHLLKVQRQDGKDEIIKNVPLKKMADRIRKYQILNNEVFAILNKYMKSVETDSSTVEHVRCFQPPIHQSLATTC wasf1_bovin Wiskott-Aldrich syndrome protein family member 1 WASF1 Protein WAVE-1 protein protein bovin Bos taurus (Bovine) Bovine MPLVKRNIDPRHLCHTALPRGIKNELECVTNISLANIIRQLSSLSKYAEDIFGELFNEAHSFSFRVNSLQERVDRLSVSVTQLDPKEEELSLQDITMRKAFRSSTIQDQQLFDRKTLPIPLQETYDVCEQPPPLNILTPYRDDGKEGLKFYTNPSYFFDLWKEKMLQDTEDKRKEKRKQKQKNLDRPHEPEKVPRAPHDRRREWQKLAQGPELAEDDANLLHKHIEVANGPASHFETRPQTYVDHMDGSYSLSALPFSQMSELLTRAEERVLVRPHEPPPPPPMHGAGDAKPIPTCISSATGLIENRPQSPATGRTPVFVSPTPPPPPPPLPSALSTSSLRASMTSTPPPPVPPPPPPPTTALQAPAVPPPPAPLQIAPGVLHPAPPPIAPPLVQPSPPVARAAPVCETVPVHPLPQGEVQGLPPPPPPPPLPPPGIRPSSPVTVAALAHPPSGLHPTPSTAPGPHVPLMPPSPPSQVTPASEPKRHPSTLPVISDARSVLLEAIRKGIQLRKVEEQREQEAKHERIENDVATILSRRIAVEYSDSEDDSEFDEVDWLE rac1_human Ras-related C3 botulinum toxin substrate 1 RAC1 MIG5 p21-Rac1 Ras-like protein TC25 Cell migration-inducing gene 5 protein TC25 protein protein human Homo sapiens Human MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL c22h3orf10-cyfip2 1300808 n/a 1300811 unspecified role unspecified role neutral component neutral component n/a 1300812 unspecified role unspecified role neutral component neutral component n/a 1300813 unspecified role unspecified role neutral component neutral component n/a 1300814 unspecified role unspecified role neutral component neutral component association association false false false Fig 1a (left). wasf1-c22h3orf10-1 1300809 n/a 1300811 unspecified role unspecified role prey prey n/a 1300814 unspecified role unspecified role bait bait n/a 1300812 unspecified role unspecified role prey prey n/a 1300813 unspecified role unspecified role prey prey association association false false false Fig 1a (Right). rac1-cyfip2-1 1300810 n/a 1300815 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1300813 unspecified role unspecified role prey prey bovin-brain Bos taurus brain brain Brain [cerebrum] n/a 1300812 unspecified role unspecified role prey prey bovin-brain Bos taurus brain brain Brain [cerebrum] association association false false false Fig 3d. The Wave complex subunits (PIR121 and NAP125) bound tightly to GTP-bound Rac1 whereas the interaction was very weak with GDP-bound Rac1.