IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ zhao-2002-1 Structure and catalytic mechanism of the E. coli chemotaxis phosphatase CheZ. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Zhao R., Collins E.J., Bourret R.B., Silversmith R.E. silversr@med.unc.edu Nat. Struct. Biol. (1072-8368) 2002 Only protein-protein interactions imex curation zhao-2002-2 Structure and catalytic mechanism of the E. coli chemotaxis phosphatase CheZ. in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Zhao R., Collins E.J., Bourret R.B., Silversmith R.E. silversr@med.unc.edu Nat. Struct. Biol. (1072-8368) 2002 Only protein-protein interactions imex curation chey_ecoli Chemotaxis protein CheY cheY b1882 JW1871 protein protein ecoli Escherichia coli (strain K12) MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALNKLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAGASGYVVKPFTAATLEEKLNKIFEKLGM chez_ecoli Protein phosphatase CheZ cheZ b1881 JW1870 Chemotaxis protein CheZ protein protein ecoli Escherichia coli (strain K12) MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEAAEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPIDLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIERQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF chey-chez-2 1345461 n/a 1345463 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1345464 unspecified role unspecified role neutral component neutral component P0A9H9:p.Glu134Lys mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Figure 1 2.9 A R value: 0.279 R free: 0.298 chey-chez-4 1345462 n/a 1345464 competitor competitor competitor neutral component neutral component P0A9H9:p.Gln147Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1345463 competitor competitor competitor neutral component neutral component P0AE67:p.Asn59Arg mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1345464 unspecified role unspecified role neutral component neutral component fluorescein fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Figure 2C