IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ song-2001b-1 Carboxyl-terminal domain III of the delta' subunit of DNA polymerase III holoenzyme binds DnaX and supports cooperative DnaX complex assembly. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Song M.S., McHenry C.S. charles.mchenry@uchsc.edu J. Biol. Chem. (0021-9258) 2001 Only protein-protein interactions imex curation song-2001b-2 Carboxyl-terminal domain III of the delta' subunit of DNA polymerase III holoenzyme binds DnaX and supports cooperative DnaX complex assembly. in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Song M.S., McHenry C.S. charles.mchenry@uchsc.edu J. Biol. Chem. (0021-9258) 2001 Only protein-protein interactions imex curation p06710-1 DNA polymerase III subunit tau dnaX dnaZ dnaZX DNA polymerase III subunit gamma b0470 JW0459 protein protein ecoli Escherichia coli (strain K12) MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFSGTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVEDTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQKLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDALSLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGIEWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLIGRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQPQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQTLRRFFDAELDEESIRPI Produced by full-length translation of the dnaX gene. This isoform is essential, constructs that express only the gamma isoform are not viable (Ref.14). holb_ecoli DNA polymerase III subunit delta' holB b1099 JW1085 protein protein ecoli Escherichia coli (strain K12) MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYALSRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGVDAVREVTEKLNEHARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCRLHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAELANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL hold_ecoli DNA polymerase III subunit psi holD b4372 JW4334 protein protein ecoli Escherichia coli (strain K12) MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVANDLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSHCNSWRLGTDEPLSLEGAQVASPALTDLRANPTARAALWQQICTYEHDFFPRND hola_ecoli DNA polymerase III subunit delta b0640 holA JW0635 protein protein ecoli Escherichia coli (strain K12) MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAAQGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTGLLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNLELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWVDALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKHRVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHKPLADVFIDG 87239A678FE16BE2 holc_ecoli DNA polymerase III subunit chi holC b4259 JW4216 protein protein ecoli Escherichia coli (strain K12) MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACEDEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSRRDILISLRTSFADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK 7FEFFC359B28AA88 p06710-2 DNA polymerase III subunit tau dnaX dnaZ dnaZX b0470 JW0459 DNA polymerase III subunit gamma protein protein ecoli Escherichia coli (strain K12) MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFSGTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVEDTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQKLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDALSLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGIEWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLIGRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQPQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKE Formed by programmed ribosomal frameshifting to a premature stop codon in the -1 frame at codon 430, the last residue is thus Glu and not Ser. Mutants which remove the frameshift are viable, suggesting strongly that gamma is not essential for viability (Ref.14). tau-holb 1349688 n/a 1349690 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1349691 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminal biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 1c full tau complex 1349688 n/a 1349690 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1349691 unspecified role unspecified role neutral component neutral component 207-334 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false n-terminal biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1349692 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1349693 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1349694 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 2b, c, 3a, Table I Subunits holA (delta), holC (chi) and holD (psi) stabilise binding of holB (delta') and dnaX (tau). Table I also shows kinetics for binding of s/u delta' domain III only to the tau complex. tau complex, part of DNA Polymerase III complex holc-hold-tau 1349688 n/a 1349694 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1349690 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1349692 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 2a, 3c Subunit holB (delta') is required for binding of s/u holA (delta) to the rest of the tau complex. full gamma complex 1349688 n/a 1349695 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1349694 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1349693 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1349691 unspecified role unspecified role neutral component neutral component n-terminal biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false 207-334 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1349692 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 3b Subunits holA (delta), holC (chi) and holD (psi) stabilise binding of holB (delta') and dnaX (tau). gamma complex, part of DNA Polymerase III complex holc-hold-gamma 1349688 n/a 1349695 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1349694 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1349692 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 3d Subunit holB (delta') is required for binding of s/u holA (delta) to the rest of the gamma complex. full tau complex 1349689 n/a 1349694 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) n/a 1349693 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) n/a 1349691 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) n/a 1349692 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) n/a 1349690 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) physical association physical association false false false 1349689 Fig. 4a Subunits holA (delta), holC (chi) and holD (psi) stabilise binding of holB (delta') and dnaX (tau). Table I also shows kinetics for binding of s/u delta' domain III only to the tau complex. tau complex, part of DNA Polymerase III complex hola-holb-tau 1349689 n/a 1349691 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) n/a 1349690 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) n/a 1349693 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) physical association physical association false false false 1349689 Fig. 4a Subunits holA (delta), holC (chi) and holD (psi) stabilise binding of holB (delta') and dnaX (tau). Table I also shows kinetics for binding of s/u delta' domain III only to the tau complex. tau complex, part of DNA Polymerase III complex holb-tau 1349689 n/a 1349690 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) n/a 1349691 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) physical association physical association false false false 1349689 Fig. 4a Subunits holA (delta), holC (chi) and holD (psi) stabilise binding of holB (delta') and dnaX (tau). Table I also shows kinetics for binding of s/u delta' domain III only to the tau complex. tau complex, part of DNA Polymerase III complex