mbinfo MBInfo http://www.mechanobio.info/ http://www.mechanobio.info/ nikolopoulos-2000-1 Actopaxin, a new focal adhesion protein that binds paxillin LD motifs and actin and regulates cell adhesion. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Nikolopoulos S.N., Turner C.E. Could not curate interactions from Fig 4C, 5, 6, 7A, 8 and 9 since the source of recombinant actopaxin proteins (GST-actopaxin, GFP-actopaxin, Xpress-tagged actopaxin) was not specified in the paper and could not be traced. interaction detection method: GST pull down. The source of actopaxin inferred from the cell lysates (REF-52) used and the source of paxillin inferred from the amino acid (559) length specified by the author. turnerc@mail.upstate.edu J. Cell Biol. (0021-9525) 2000 Only protein-protein interactions imex curation nikolopoulos-2000-2 Actopaxin, a new focal adhesion protein that binds paxillin LD motifs and actin and regulates cell adhesion. human-intestinal muscle Human intestinal smooth mucle (HISM) intestinal_muscle intestinal muscle anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Nikolopoulos S.N., Turner C.E. Could not curate interactions from Fig 4C, 5, 6, 7A, 8 and 9 since the source of recombinant actopaxin proteins (GST-actopaxin, GFP-actopaxin, Xpress-tagged actopaxin) was not specified in the paper and could not be traced. interaction detection method: GST pull down. The source of actopaxin inferred from the cell lysates (REF-52) used and the source of paxillin inferred from the amino acid (559) length specified by the author. turnerc@mail.upstate.edu J. Cell Biol. (0021-9525) 2000 Only protein-protein interactions imex curation paxi_chick Paxillin PXN protein protein chick Gallus gallus (Chicken) Chicken MDDLDALLADLESTTSHISKRPVFLTEETPYSYPTGNHTYQEIAVPPPVPPPPSSEALNGTVIDPLDQWQPSVSRYGHQQPQSQSPIYSSSAKSSSASVPRDGLSSPSPRASEEEHVYSFPNKQKSAEPSPTMTSTSLGSNLSELDRLLLELNAVQHNPPSGFSADEVSRSPSLPNVTGPHYVIPESSSSAGGKAAPPTKEKPKRNGGRGIEDVRPSVESLLDELESSVPSPVPAITVSQGEVSSPQRVNASQQQTRISASSATRELDELMASLSDFKFMAQGKAGGSSSPPSTTPKPGSQLDTMLGSLQSDLNKLGVATVAKGVCGACKKPIAGQVVTAMGKTWHPEHFVCTHCQEEIGSRNFFERDGQPYCEKDYHNLFSPRCYYCNGPILDKVVTALDRTWHPEHFFCAQCGVFFGPEGFHEKDGKAYCRKDYFDMFAPKCGGCARAILENYISALNTLWHPECFVCRECFTPFINGSFFEHDGQPYCEVHYHERRGSLCSGCQKPITGRCITAMGKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQNCFLKLFC parva_rat Alpha-parvin Parva Actp Actopaxin protein protein rat Rattus norvegicus (Rat) Rat MATSPQKSPSVPKSPTPKSPPSRKKDDSFLGKLGGTLARRKKAKEVSEFQEEGMNAINLPLSPISFELDPEDTMLEENEVRTMVDPNSRNDPKLQELMKVLIDWINDVLVGERIIVKDLAEDLYDGQVLQKLFEKLESEKLNVAEVTQSEIAQKQKLQTVLEKINEALKLPPRSIKWNVDSVHAKNLVAILHLLVALSQYFRAPIRLPDHVSIQVVVVQKREGILQSRQIQEEITGNTEALSGRHERDAFDTLFDHAPDKLNVVKKTLITFVNKHLNKLNLEVTELETQFADGVYLVLLMGLLEGYFVPLHSFFLTPDSFEQKVLNVSFAFELMQDGGLEKPKPRPEDIVNCDLKSTLRVLYNLFTKYRNVE parva_human Alpha-parvin Calponin-like integrin-linked kinase-binding protein CH-ILKBP Matrix-remodeling-associated protein 2 Actopaxin PARVA MXRA2 protein protein human Homo sapiens Human MATSPQKSPSVPKSPTPKSPPSRKKDDSFLGKLGGTLARRKKAKEVSELQEEGMNAINLPLSPIPFELDPEDTMLEENEVRTMVDPNSRSDPKLQELMKVLIDWINDVLVGERIIVKDLAEDLYDGQVLQKLFEKLESEKLNVAEVTQSEIAQKQKLQTVLEKINETLKLPPRSIKWNVDSVHAKSLVAILHLLVALSQYFRAPIRLPDHVSIQVVVVQKREGILQSRQIQEEITGNTEALSGRHERDAFDTLFDHAPDKLNVVKKTLITFVNKHLNKLNLEVTELETQFADGVYLVLLMGLLEGYFVPLHSFFLTPDSFEQKVLNVSFAFELMQDGGLEKPKPRPEDIVNCDLKSTLRVLYNLFTKYRNVE paxi_human Paxillin PXN protein protein human Homo sapiens Human MDDLDALLADLESTTSHISKRPVFLSEETPYSYPTGNHTYQEIAVPPPVPPPPSSEALNGTILDPLDQWQPSSSRFIHQQPQSSSPVYGSSAKTSSVSNPQDSVGSPCSRVGEEEHVYSFPNKQKSAEPSPTVMSTSLGSNLSELDRLLLELNAVQHNPPGFPADEANSSPPLPGALSPLYGVPETNSPLGGKAGPLTKEKPKRNGGRGLEDVRPSVESLLDELESSVPSPVPAITVNQGEMSSPQRVTSTQQQTRISASSATRELDELMASLSDFKIQGLEQRADGERCWAAGWPRDGGRSSPGGQDEGGFMAQGKTGSSSPPGGPPKPGSQLDSMLGSLQSDLNKLGVATVAKGVCGACKKPIAGQVVTAMGKTWHPEHFVCTHCQEEIGSRNFFERDGQPYCEKDYHNLFSPRCYYCNGPILDKVVTALDRTWHPEHFFCAQCGAFFGPEGFHEKDGKAYCRKDYFDMFAPKCGGCARAILENYISALNTLWHPECFVCRECFTPFVNGSFFEHDGQPYCEVHYHERRGSLCSGCQKPITGRCITAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQNCFLKLFC pxn-parva-1 1293702 n/a 1293704 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ld1 motif sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false The sequence range for the paxillin LD1 motif was not given by the author and also could not be found in the chick paxillin swissprot entry. ld4 motif sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false The sequence range for paxillin LD4 motif was not given by the author and also could not be found on the chick paxillin swisprot entry page. ecoli Escherichia coli (strain K12) n/a 1293705 unspecified role unspecified role prey prey rat-embryonic fibroblast Rat embryonic fibroblast embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. physical association physical association false false false Fig 4B (top lane). actopaxin expressed in REF-52 cells. parva-pxn-1 1293703 n/a 1293706 unspecified role unspecified role bait bait n/a 1293707 unspecified role unspecified role prey prey physical association physical association false false false Fig 7B.