MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint t_rkeri-2012-1 Phylogenetic and functional features of the plastid transcription kinase cpCK2 from Arabidopsis signify a role of cysteinyl SH-groups in regulatory phosphorylation of plastid sigma factors. in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Türkeri H., Schweer J., Link G. FEBS J. (1742-464X) 2012 t_rkeri-2012-2 Phylogenetic and functional features of the plastid transcription kinase cpCK2 from Arabidopsis signify a role of cysteinyl SH-groups in regulatory phosphorylation of plastid sigma factors. in vitro In vitro comigration in gel comigration in gel electrophoresis comigration in gel predetermined predetermined participant predetermined Türkeri H., Schweer J., Link G. FEBS J. (1742-464X) 2012 o49935_sinal Sig1 protein protein sinal Sinapis alba (White mustard) Brassica hirta White mustard MATTAVIGLNAGKRLLSSSFYYSDVTEKFLSVNDLCSSQYHTRSGITAKKTSSSSSSNYSPSFPSSSRQTQSAKALKESVASSVQPWLPPGSDQELEEDDIDHSVEALLLLQRSMLEKQWNLSFEKTRKKVPVTCSGISARQRRIGAKKKTNVKAVSEVNFQNNLVKGYVKGVISDHVLSHAEVVRLSKKIKSGLRLDEHKSRLTDRLGCEPSDEQLAMSLKISRAELQAWLMECHLAREKLAMSNVRLVMSIAQRYDNMGAEMSDLVQGGLIGLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALVDNSRTLRLPTHLHERLGLIRNAKLRLQEKGITPSIDRIAESLNMSQKKVRNATEAVSKIFSLDRDAFPSLNGLPGETHHSYIADNRLENNPWHGYDALALKDEVSKLISVTLGEREREIIRLYYGLDKECLTWEDISQRIGLSRERVRQVGLVALEKLKHAARKRKMEAMILKN mint csk2p_arath Casein kinase II subunit alpha-4, chloroplastic CKA4 Plastid-targeted casein kinase 2 alpha F21P24.13 At2g23070 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MALRPCTGFTISSLRNASAANNNLFSLLSFSSSSPAKRNLLLSSLQDNLRRFASSASLYRQHLRNQQQQHQQQQQSRVKEKSETLAQKIGKSIRRAGAPSKARVYADVNVVRPKDYWDYESLAVQWGVQDDYEVVRKVGRGKYSEVFEGIHATDNEKCVIKILKPVKKKKIKREIKILQNLCGGPNIVKLLDIVRDQQSKTPSLIFEHVNNKDFKVLYPTLSDYDVRYYIFELLKALDFCHSRGIMHRDVKPHNVMIDHEQRKLRLIDWGLAEFYHPGKEYNVRVASRYFKGPELLVDLQDYDYSLDLWSLGCMFAGMIFRKEPFFYGHDNYDQLVKIAKVLGTDELNAYLNKYRIELDPNLTSLVGRHSRKPWTKFINSENQHLAVPEAVDFVDKLLRYDHQERPTAKEAMAHPYFYPIRNAESSRTPRSQ q9mth3_sinal RNA polymerase sigma factor sig2 protein protein sinal Sinapis alba (White mustard) Brassica hirta White mustard MSSCLLPNFKCQPDSFSVHFRTSYSVSKHSKGSAFFQPQCAVSTSPPLLTSTLDVAKLRLPSFDTDSDSRISDRPWTYTRTIGPSTEAKYLEALASETLLTSDEAVVAAAAAEAVALARAALKVAKDATLFGNSHNTKLSVSSAADKRSKWDQFTEKERAGILGHLAVSDTGVVSDRITAPGSLECNKEASDDLTPAEKQEVEKLSVSSAVRSTRQTERKARRVKGLEKTASGMQSSVKTGSSSRKKRVASQEIDHNDPLRYLRMTTSSSKLLTAREEQQLSEGIQDLLKLERLQAELTERCGHQPTFSQWASAAGVDKKTLRKRITHGTQCKDRMIKSNIRLVISIAKNYQGAGMDFQDLVQEGCRGLVRGAEKFDATKGFKFSTYAHWWIKQAVRKSLSDQSRMIRLPFHMVEATYRVKEARKQLYSETGKQPKNEEVAEATGLSMKRLMAVLLSPKPPRSLDQKIGINLNLKPSEVIADPEAETSEDILMKQFMRQDLDKVLDSLSTREKQVIRWRFGMEDGRMKTLQEIGETMGVSRERVRQIDTSAFRKLKNKKRNSHLQQYLVAQTKES mint siga_arath RNA polymerase sigma factor sigA SIGA RNA polymerase sigma factor sig1 RNA polymerase sigma factor sig2 RNA polymerase sigma factor sigB RPOD1 SIG1 SIG2 SIGB F13O11.16 At1g64860 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MATAAVIGLNTGKRLLSSSFYHSDVTEKFLSVNDHCSSQYHIASTKSGITAKKASNYSPSFPSSNRHTQSAKALKESVDVASTEKPWLPNGTDKELEEECYDDDDLISHSVEAILLLQKSMLEKSWNLSFEKAVSSEYPGKGTIRKKKIPVITCSGISARQRRIGAKKKTNMTHVKAVSDVSSGKQVRGYVKGVISEDVLSHVEVVRLSKKIKSGLRLDDHKSRLKDRLGCEPSDEQLAVSLKISRAELQAWLMECHLAREKLAMSNVRLVMSIAQRYDNLGAEMSDLVQGGLIGLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALVDNSRTLRLPTHLHERLGLIRNAKLRLQEKGITPSIDRIAESLNMSQKKVRNATEAVSKVFSLDRDAFPSLNGLPGETHHSYIADTRLENNPWHGYDDLALKEEVSKLISATLGEREKEIIRLYYGLDKECLTWEDISKRIGLSRERVRQVGLVALEKLKHAARKRKMEAMILKN sigf_arath RNA polymerase sigma factor sigF, chloroplastic SIGF Protein SINGLET OXYGEN-LINKED DEATH ACTIVATOR 8 RNA polymerase sigma factor sig6 SIG6 SOLDAT8 T1J8 At2g36990 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEATRNLVSSSPSFQTKTHLKSSYSSPSSVVMLHDQTTTPVVNSRHLNSLSRHFPASVLSQEPREESRPLSHALRDDRTSQLTLERRQFDELVSSREDEKFEQQLLHSTGLWNLLISPLTSETKLPAVVSPLADAELCDVVALAQKALSASKQAALLVDDTEANPSDNIKDSLSTSSSMSLPEKGNIVRSKRQLERRAKNRRAPKSNDVDDEGYVPQKTSAKKKYKQGADNDDALQLFLWGPETKQLLTAKEEAELISHIQHLLKLEKVKTKLESQNGCEPTIGEWAEAMGISSPVLKSDIHRGRSSREKLITANLRLVVHIAKQYQNRGLNFQDLLQEGSMGLMKSVEKFKPQSGCRFATYAYWWIRQSIRKSIFQNSRTIRLPENVYMLLGKVSEARKTCVQEGNYRPSKEELAGHVGVSTEKLDKLLYNTRTPLSMQQPIWSDQDTTFQEITPDSGIETPTMSVGKQLMRNHVRNLLNVLSPKERRIIKLRFGIDGGKQRSLSEIGEIYGLSKERVRQLESRALYRLKQNMNSHGLHAYADLLV csk2p-sig1 1241344 sig1 SaSig1 1241346 weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra enzyme target enzyme target substrate substrate neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false casein kinase 2 cpCK2 1241347 predetermined predetermined participant predetermined enzyme enzyme neutral component neutral component purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false phosphorylation phosphorylation reaction phosphorylation false false false f2b mint csk2p-sig2 1241344 sig2 SaSig2 1241348 weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra enzyme target enzyme target substrate substrate neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false casein kinase 2 cpCK2 1241347 predetermined predetermined participant predetermined enzyme enzyme neutral component neutral component purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false phosphorylation phosphorylation reaction phosphorylation false false false f2b mint csk2p-siga 1241344 casein kinase 2 cpCK2 1241347 predetermined predetermined participant predetermined enzyme enzyme neutral component neutral component purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false sig1 AtSig1 1241349 weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra enzyme target enzyme target substrate substrate neutral component neutral component purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false phosphorylation phosphorylation reaction phosphorylation false false false f2b mint csk2p-sigf 1241344 casein kinase 2 cpCK2 1241347 predetermined predetermined participant predetermined enzyme enzyme neutral component neutral component purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false sig6 AtSig6 1241350 weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra enzyme target enzyme target substrate substrate neutral component neutral component purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false phosphorylation phosphorylation reaction phosphorylation false false false f2b mint csk2p-1 1241345 casein kinase 2 cpCK2 1241347 western blot western blot Immuno blot Immuno blot unspecified role unspecified role neutral component neutral component purified purified O64816:p.Cys182Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 direct interaction direct interaction false false false f5 mint