IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ kim-2011d-5 SIRT2 Maintains Genome Integrity and Suppresses Tumorigenesis through Regulating APC/C Activity. mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kim H.S., Vassilopoulos A., Wang R.H., Lahusen T., Xiao Z., Xu X., Li C., Veenstra T.D., Li B., Yu H., Ji J., Wang X.W., Park S.H., Cha Y.I., Gius D., Deng C.X. Cancer - Interactions investigated in the context of cancer The protein referred to as "CDH1" was identified as (Gene name) FZR1 - Fizzy-related protein homolog/Cdh1/Hct1 homolog, rather than (Gene name) CDH1 -Cadherin-1/CD324, on the basis of sequence/mutation location info given in this paper. Cdh1-HA/Myc/Flag and Cdc20-HA/Myc/Flag constructs were found to be Human in origin, according to the Addgene web site. chuxiad@bdg10.niddk.nih.gov Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation kim-2011d-4 SIRT2 Maintains Genome Integrity and Suppresses Tumorigenesis through Regulating APC/C Activity. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kim H.S., Vassilopoulos A., Wang R.H., Lahusen T., Xiao Z., Xu X., Li C., Veenstra T.D., Li B., Yu H., Ji J., Wang X.W., Park S.H., Cha Y.I., Gius D., Deng C.X. Cancer - Interactions investigated in the context of cancer The authors have confirmed that the IPs shown in Figs 6A/B were carried out using HeLa cells. The protein referred to as "CDH1" was identified as (Gene name) FZR1 - Fizzy-related protein homolog/Cdh1/Hct1 homolog, rather than (Gene name) CDH1 -Cadherin-1/CD324, on the basis of sequence/mutation location info given in this paper. Cdh1-HA/Myc/Flag and Cdc20-HA/Myc/Flag constructs were found to be Human in origin, according to the Addgene web site. chuxiad@bdg10.niddk.nih.gov Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation kim-2011d-6 SIRT2 Maintains Genome Integrity and Suppresses Tumorigenesis through Regulating APC/C Activity. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kim H.S., Vassilopoulos A., Wang R.H., Lahusen T., Xiao Z., Xu X., Li C., Veenstra T.D., Li B., Yu H., Ji J., Wang X.W., Park S.H., Cha Y.I., Gius D., Deng C.X. Cancer - Interactions investigated in the context of cancer The protein referred to as "CDH1" was identified as (Gene name) FZR1 - Fizzy-related protein homolog/Cdh1/Hct1 homolog, rather than (Gene name) CDH1 -Cadherin-1/CD324, on the basis of sequence/mutation location info given in this paper. Cdh1-HA/Myc/Flag and Cdc20-HA/Myc/Flag constructs were found to be Human in origin, according to the Addgene web site. chuxiad@bdg10.niddk.nih.gov Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation kim-2011d-8 SIRT2 Maintains Genome Integrity and Suppresses Tumorigenesis through Regulating APC/C Activity. in vitro In vitro deacetylase assay deacetylase assay western blot western blot Immuno blot Immuno blot Kim H.S., Vassilopoulos A., Wang R.H., Lahusen T., Xiao Z., Xu X., Li C., Veenstra T.D., Li B., Yu H., Ji J., Wang X.W., Park S.H., Cha Y.I., Gius D., Deng C.X. Cancer - Interactions investigated in the context of cancer The authors confirm that Myc-Cdh1/Cdc20 were used in this Experiment, rather than HA-Cdh1/Cdc20; also that SIRT-2 and mutants have an "HA" tag and are Human in origin. The protein referred to as "CDH1" was identified as (Gene name) FZR1 - Fizzy-related protein homolog/Cdh1/Hct1 homolog, rather than (Gene name) CDH1 -Cadherin-1/CD324, on the basis of sequence/mutation location info given in this paper. Cdh1-HA/Myc/Flag and Cdc20-HA/Myc/Flag constructs were found to be Human in origin, according to the Addgene web site. chuxiad@bdg10.niddk.nih.gov Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation kim-2011d-9 SIRT2 Maintains Genome Integrity and Suppresses Tumorigenesis through Regulating APC/C Activity. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. deacetylase assay deacetylase assay western blot western blot Immuno blot Immuno blot Kim H.S., Vassilopoulos A., Wang R.H., Lahusen T., Xiao Z., Xu X., Li C., Veenstra T.D., Li B., Yu H., Ji J., Wang X.W., Park S.H., Cha Y.I., Gius D., Deng C.X. Cancer - Interactions investigated in the context of cancer The protein referred to as "CDH1" was identified as (Gene name) FZR1 - Fizzy-related protein homolog/Cdh1/Hct1 homolog, rather than (Gene name) CDH1 -Cadherin-1/CD324, on the basis of sequence/mutation location info given in this paper. Cdh1-HA/Myc/Flag and Cdc20-HA/Myc/Flag constructs were found to be Human in origin, according to the Addgene web site. chuxiad@bdg10.niddk.nih.gov Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation kim-2011d-1 SIRT2 Maintains Genome Integrity and Suppresses Tumorigenesis through Regulating APC/C Activity. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kim H.S., Vassilopoulos A., Wang R.H., Lahusen T., Xiao Z., Xu X., Li C., Veenstra T.D., Li B., Yu H., Ji J., Wang X.W., Park S.H., Cha Y.I., Gius D., Deng C.X. Cancer - Interactions investigated in the context of cancer The protein referred to as "CDH1" was identified as (Gene name) FZR1 - Fizzy-related protein homolog/Cdh1/Hct1 homolog, rather than (Gene name) CDH1 -Cadherin-1/CD324, on the basis of sequence/mutation location info given in this paper. Cdh1-HA/Myc/Flag and Cdc20-HA/Myc/Flag constructs were found to be Human in origin, according to the Addgene web site. chuxiad@bdg10.niddk.nih.gov Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation kim-2011d-2 SIRT2 Maintains Genome Integrity and Suppresses Tumorigenesis through Regulating APC/C Activity. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kim H.S., Vassilopoulos A., Wang R.H., Lahusen T., Xiao Z., Xu X., Li C., Veenstra T.D., Li B., Yu H., Ji J., Wang X.W., Park S.H., Cha Y.I., Gius D., Deng C.X. Cancer - Interactions investigated in the context of cancer The protein referred to as "CDH1" was identified as (Gene name) FZR1 - Fizzy-related protein homolog/Cdh1/Hct1 homolog, rather than (Gene name) CDH1 -Cadherin-1/CD324, on the basis of sequence/mutation location info given in this paper. Cdh1-HA/Myc/Flag and Cdc20-HA/Myc/Flag constructs were found to be Human in origin, according to the Addgene web site. chuxiad@bdg10.niddk.nih.gov Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation kim-2011d-3 SIRT2 Maintains Genome Integrity and Suppresses Tumorigenesis through Regulating APC/C Activity. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip sequence tag sequence tag identification sequence tag Kim H.S., Vassilopoulos A., Wang R.H., Lahusen T., Xiao Z., Xu X., Li C., Veenstra T.D., Li B., Yu H., Ji J., Wang X.W., Park S.H., Cha Y.I., Gius D., Deng C.X. Cancer - Interactions investigated in the context of cancer The protein referred to as "CDH1" was identified as (Gene name) FZR1 - Fizzy-related protein homolog/Cdh1/Hct1 homolog, rather than (Gene name) CDH1 -Cadherin-1/CD324, on the basis of sequence/mutation location info given in this paper. Cdh1-HA/Myc/Flag and Cdc20-HA/Myc/Flag constructs were found to be Human in origin, according to the Addgene web site. chuxiad@bdg10.niddk.nih.gov Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation kim-2011d-7 SIRT2 Maintains Genome Integrity and Suppresses Tumorigenesis through Regulating APC/C Activity. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Kim H.S., Vassilopoulos A., Wang R.H., Lahusen T., Xiao Z., Xu X., Li C., Veenstra T.D., Li B., Yu H., Ji J., Wang X.W., Park S.H., Cha Y.I., Gius D., Deng C.X. Cancer - Interactions investigated in the context of cancer Aurora A was detected by immunostaining. The protein referred to as "CDH1" was identified as (Gene name) FZR1 - Fizzy-related protein homolog/Cdh1/Hct1 homolog, rather than (Gene name) CDH1 -Cadherin-1/CD324, on the basis of sequence/mutation location info given in this paper. Cdh1-HA/Myc/Flag and Cdc20-HA/Myc/Flag constructs were found to be Human in origin, according to the Addgene web site. chuxiad@bdg10.niddk.nih.gov Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation fzr1_mouse Fizzy-related protein homolog Fzr1 Fyr Fzr Cdh1/Hct1 homolog protein protein mouse Mus musculus Mouse MDQDYERRLLRQIIIQNENTVPCVSEMRRTLTPANSPVSSPSKHGDRFIPSRAGANWSVNFHRINENEKSPSQNRKAKDATSDNGKDGLAYSALLKNELLGAGIEKVQDPQTEDRRLQPSTPEHKGLFTYSLSSKRSSPDDGNDVSPYSLSPVSNKSQKLLRSPRKPTRKISKIPFKVLDAPELQDDFYLNLVDWSSLNVLSVGLGTCVYLWSACTSQVTRLCDLSVEGDSVTSVGWSERGNLVAVGTHKGFVQIWDAAAGKKLSMLEGHTARVGALAWNADQLSSGSRDRMILQRDIRTPPLQSERRLQGHRQEVCGLKWSTDHQLLASGGNDNKLLVWNHSSLSPVQQYTEHLAAVKAIAWSPHQHGLLASGGGTADRCIRFWNTLTGQPLQCIDTGSQVCNLAWSKHANELVSTHGYSQNQILVWKYPSLTQVAKLTGHSYRVLYLAMSPDGEAIVTGAGDETLRFWNVFSKTRSTKESVSVLNLFTRIR sir2_mouse NAD-dependent protein deacetylase sirtuin-2 SIR2-like protein 2 Sirt2 Sir2l2 Regulatory protein SIR2 homolog 2 protein protein mouse Mus musculus Mouse MAEPDPSDPLETQAGKVQEAQDSDSDTEGGATGGEAEMDFLRNLFTQTLGLGSQKERLLDELTLEGVTRYMQSERCRKVICLVGAGISTSAGIPDFRSPSTGLYANLEKYHLPYPEAIFEISYFKKHPEPFFALAKELYPGQFKPTICHYFIRLLKEKGLLLRCYTQNIDTLERVAGLEPQDLVEAHGTFYTSHCVNTSCRKEYTMGWMKEKIFSEATPRCEQCQSVVKPDIVFFGENLPSRFFSCMQSDFSKVDLLIIMGTSLQVQPFASLISKAPLATPRLLINKEKTGQTDPFLGMMMGLGGGMDFDSKKAYRDVAWLGDCDQGCLALADLLGWKKELEDLVRREHANIDAQSGSQAPNPSTTISPGKSPPPAKEAARTKEKEEQQ cdc20_mouse Cell division cycle protein 20 homolog p55CDC Cdc20 protein protein mouse Mus musculus Mouse MAQFVFESDLHSLLQLDAPIPNAPVARWQRKAKEATGPAPSPMRAANRSHSAGRTPGRTPGKSSSKVQTTPSKPGGDRFIPQRSASQMEVASFLLSKENQPEDRGTPTKKEHQKAWSLNLNGFDVEEAKILRLSGKPQNAPEGYQNRLKVLYSQKATPGSSRKTCRYIPSLPDRILDAPEIRNDYYLNLVDWSSGNVLAVALDNSVYLWNAGSGDILQLLQMEQPGDYISSVAWIKEGNYLAVGTSNAEVQLWDVQQQKRLRNMTSHSARVSSLSWNSYILSSGSRSGHIHHHDVRVAEHHVATLSGHSQEVCGLRWAPDGRHLASGGNDNIVNVWPSGPGESGWAPLQTFTQHQGAVKAVAWCPWQSNILATGGGTSDRHIRIWNVCSGACLSAVDVHSQVCSILWSPHYKELISGHGFAQNQLVIWKYPTMAKVAELKGHTARVLGLTMSPDGATVASAAADETLRLWRCFEMDPALRREREKASVAKSSLIHQGIR cdc27_mouse Cell division cycle protein 27 homolog Cdc27 protein protein mouse Mus musculus Mouse MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDLVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKLTSLQNFSSCLPNTCTTLVSNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSTVISPDNVPLGPGTAILSKQVQNKPKTGRSLLGGPTALSPLTPSFGILPLETPSPGDGSYLQNYTNTPSVIDVAPTGAPTKKSVARMGQTGTKSVFSQSGNSREVTPVLVAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGLTQPSINDSLEITKLDSSIISEGKITTVTPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYSTGWVLCQIGRAYFELSEYMQAERIFSEVRRIESFRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF fzr1_human Fizzy-related protein homolog FYR FZR KIAA1242 FZR1 CDH1 Cdh1/Hct1 homolog CDC20-like protein 1 protein protein human Homo sapiens Human MDQDYERRLLRQIVIQNENTMPRVTEMRRTLTPASSPVSSPSKHGDRFIPSRAGANWSVNFHRINENEKSPSQNRKAKDATSDNGKDGLAYSALLKNELLGAGIEKVQDPQTEDRRLQPSTPEKKGLFTYSLSTKRSSPDDGNDVSPYSLSPVSNKSQKLLRSPRKPTRKISKIPFKVLDAPELQDDFYLNLVDWSSLNVLSVGLGTCVYLWSACTSQVTRLCDLSVEGDSVTSVGWSERGNLVAVGTHKGFVQIWDAAAGKKLSMLEGHTARVGALAWNAEQLSSGSRDRMILQRDIRTPPLQSERRLQGHRQEVCGLKWSTDHQLLASGGNDNKLLVWNHSSLSPVQQYTEHLAAVKAIAWSPHQHGLLASGGGTADRCIRFWNTLTGQPLQCIDTGSQVCNLAWSKHANELVSTHGYSQNQILVWKYPSLTQVAKLTGHSYRVLYLAMSPDGEAIVTGAGDETLRFWNVFSKTRSTKVKWESVSVLNLFTRIR sir2_human NAD-dependent protein deacetylase sirtuin-2 SIR2-like protein 2 SIRT2 SIR2L SIR2L2 Regulatory protein SIR2 homolog 2 protein protein human Homo sapiens Human MAEPDPSHPLETQAGKVQEAQDSDSDSEGGAAGGEADMDFLRNLFSQTLSLGSQKERLLDELTLEGVARYMQSERCRRVICLVGAGISTSAGIPDFRSPSTGLYDNLEKYHLPYPEAIFEISYFKKHPEPFFALAKELYPGQFKPTICHYFMRLLKDKGLLLRCYTQNIDTLERIAGLEQEDLVEAHGTFYTSHCVSASCRHEYPLSWMKEKIFSEVTPKCEDCQSLVKPDIVFFGESLPARFFSCMQSDFLKVDLLLVMGTSLQVQPFASLISKAPLSTPRLLINKEKAGQSDPFLGMIMGLGGGMDFDSKKAYRDVAWLGECDQGCLALAELLGWKKELEDLVRREHASIDAQSGAGVPNPSTSASPKKSPPPAKDEARTTEREKPQ cdc20_human Cell division cycle protein 20 homolog CDC20 p55CDC protein protein human Homo sapiens Human MAQFAFESDLHSLLQLDAPIPNAPPARWQRKAKEAAGPAPSPMRAANRSHSAGRTPGRTPGKSSSKVQTTPSKPGGDRYIPHRSAAQMEVASFLLSKENQPENSQTPTKKEHQKAWALNLNGFDVEEAKILRLSGKPQNAPEGYQNRLKVLYSQKATPGSSRKTCRYIPSLPDRILDAPEIRNDYYLNLVDWSSGNVLAVALDNSVYLWSASSGDILQLLQMEQPGEYISSVAWIKEGNYLAVGTSSAEVQLWDVQQQKRLRNMTSHSARVGSLSWNSYILSSGSRSGHIHHHDVRVAEHHVATLSGHSQEVCGLRWAPDGRHLASGGNDNLVNVWPSAPGEGGWVPLQTFTQHQGAVKAVAWCPWQSNVLATGGGTSDRHIRIWNVCSGACLSAVDAHSQVCSILWSPHYKELISGHGFAQNQLVIWKYPTMAKVAELKGHTSRVLSLTMSPDGATVASAAADETLRLWRCFELDPARRREREKASAAKSSLIHQGIR aurka_human Aurora kinase A AIRK1 Serine/threonine-protein kinase 15 Aurora/IPL1-related kinase 1 Breast tumor-amplified kinase STK6 AIK ARK1 AURA BTAK STK15 AURKA AYK1 IAK1 Aurora 2 Serine/threonine-protein kinase 6 Serine/threonine-protein kinase aurora-A protein protein human Homo sapiens Human MDRSKENCISGPVKATAPVGGPKRVLVTQQFPCQNPLPVNSGQAQRVLCPSNSSQRVPLQAQKLVSSHKPVQNQKQKQLQATSVPHPVSRPLNNTQKSKQPLPSAPENNPEEELASKQKNEESKKRQWALEDFEIGRPLGKGKFGNVYLAREKQSKFILALKVLFKAQLEKAGVEHQLRREVEIQSHLRHPNILRLYGYFHDATRVYLILEYAPLGTVYRELQKLSKFDEQRTATYITELANALSYCHSKRVIHRDIKPENLLLGSAGELKIADFGWSVHAPSSRRTTLCGTLDYLPPEMIEGRMHDEKVDLWSLGVLCYEFLVGKPPFEANTYQETYKRISRVEFTFPDFVTEGARDLISRLLKHNPSQRPMLREVLEHPWITANSSKPSNCQNKESASKQS apc1_human Anaphase-promoting complex subunit 1 ANAPC1 TSG24 Cyclosome subunit 1 Testis-specific gene 24 protein Mitotic checkpoint regulator protein protein human Homo sapiens Human MSNFYEERTTMIAARDLQEFVPFGRDHCKHHPNALNLQLRQLQPASELWSSDGAAGLVGSLQEVTIHEKQKESWQLRKGVSEIGEDVDYDEELYVAGNMVIWSKGSKSQALAVYKAFTVDSPVQQALWCDFIISQDKSEKAYSSNEVEKCICILQSSCINMHSIEGKDYIASLPFQVANVWPTKYGLLFERSASSHEVPPGSPREPLPTMFSMLHPLDEITPLVCKSGSLFGSSRVQYVVDHAMKIVFLNTDPSIVMTYDAVQNVHSVWTLRRVKSEEENVVLKFSEQGGTPQNVATSSSLTAHLRSLSKGDSPVTSPFQNYSSIHSQSRSTSSPSLHSRSPSISNMAALSRAHSPALGVHSFSGVQRFNISSHNQSPKRHSISHSPNSNSNGSFLAPETEPIVPELCIDHLWTETITNIREKNSQASKVFITSDLCGQKFLCFLVESQLQLRCVKFQESNDKTQLIFGSVTNIPAKDAAPVEKIDTMLVLEGSGNLVLYTGVVRVGKVFIPGLPAPSLTMSNTMPRPSTPLDGVSTPKPLSKLLGSLDEVVLLSPVPELRDSSKLHDSLYNEDCTFQQLGTYIHSIRDPVHNRVTLELSNGSMVRITIPEIATSELVQTCLQAIKFILPKEIAVQMLVKWYNVHSAPGGPSYHSEWNLFVTCLMNMMGYNTDRLAWTRNFDFEGSLSPVIAPKKARPSETGSDDDWEYLLNSDYHQNVESHLLNRSLCLSPSEASQMKDEDFSQNLSLDSSTLLFTHIPAIFFVLHLVYEELKLNTLMGEGICSLVELLVQLARDLKLGPYVDHYYRDYPTLVRTTGQVCTIDPGQTGFMHHPSFFTSEPPSIYQWVSSCLKGEGMPPYPYLPGICERSRLVVLSIALYILGDESLVSDESSQYLTRITIAPQKLQVEQEENRFSFRHSTSVSSLAERLVVWMTNVGFTLRDLETLPFGIALPIRDAIYHCREQPASDWPEAVCLLIGRQDLSKQACEGNLPKGKSVLSSDVPSGTETEEEDDGMNDMNHEVMSLIWSEDLRVQDVRRLLQSAHPVRVNVVQYPELSDHEFIEEKENRLLQLCQRTMALPVGRGMFTLFSYHPVPTEPLPIPKLNLTGRAPPRNTTVDLNSGNIDVPPNMTSWASFHNGVAAGLKIAPASQIDSAWIVYNKPKHAELANEYAGFLMALGLNGHLTKLATLNIHDYLTKGHEMTSIGLLLGVSAAKLGTMDMSITRLLSIHIPALLPPTSTELDVPHNVQVAAVVGIGLVYQGTAHRHTAEVLLAEIGRPPGPEMEYCTDRESYSLAAGLALGMVCLGHGSNLIGMSDLNVPEQLYQYMVGGHRRFQTGMHREKHKSPSYQIKEGDTINVDVTCPGATLALAMIYLKTNNRSIADWLRAPDTMYLLDFVKPEFLLLRTLARCLILWDDILPNSKWVDSNVPQIIRENSISLSEIELPCSEDLNLETLSQAHVYIIAGACLSLGFRFAGSENLSAFNCLHKFAKDFMTYLSAPNASVTGPHNLETCLSVVLLSLAMVMAGSGNLKVLQLCRFLHMKTGGEMNYGFHLAHHMALGLLFLGGGRYSLSTSNSSIAALLCALYPHFPAHSTDNRYHLQALRHLYVLAAEPRLLVPVDVDTNTPCYALLEVTYKGTQWYEQTKEELMAPTLLPELHLLKQIKVKGPRYWELLIDLSKGTQHLKSILSKDGVLYVKLRAGQLSYKEDPMGWQSLLAQTVANRNSEARAFKPETISAFTSDPALLSFAEYFCKPTVNMGQKQEILDLFSSVLYECVTQETPEMLPAYIAMDQAIRRLGRREMSETSELWQIKLVLEFFSSRSHQERLQNHPKRGLFMNSEFLPVVKCTIDNTLDQWLQVGGDMCVHAYLSGQPLEESQLSMLACFLVYHSVPAPQHLPPIGLEGSTSFAELLFKFKQLKMPVRALLRLAPLLLGNPQPMVM cdc23_human Cell division cycle protein 23 homolog CDC23 ANAPC8 Anaphase-promoting complex subunit 8 Cyclosome subunit 8 protein protein human Homo sapiens Human MAASTSMVPVAVTAAVAPVLSINSDFSDLREIKKQLLLIAGLTRERGLLHSSKWSAELAFSLPALPLAELQPPPPITEEDAQDMDAYTLAKAYFDVKEYDRAAHFLHGCNSKKAYFLYMYSRYLSGEKKKDDETVDSLGPLEKGQVKNEALRELRVELSKKHQARELDGFGLYLYGVVLRKLDLVKEAIDVFVEATHVLPLHWGAWLELCNLITDKEMLKFLSLPDTWMKEFFLAHIYTELQLIEEALQKYQNLIDVGFSKSSYIVSQIAVAYHNIRDIDKALSIFNELRKQDPYRIENMDTFSNLLYVRSMKSELSYLAHNLCEIDKYRVETCCVIGNYYSLRSQHEKAALYFQRALKLNPRYLGAWTLMGHEYMEMKNTSAAIQAYRHAIEVNKRDYRAWYGLGQTYEILKMPFYCLYYYRRAHQLRPNDSRMLVALGECYEKLNQLVEAKKCYWRAYAVGDVEKMALVKLAKLHEQLTESEQAAQCYIKYIQDIYSCGEIVEHLEESTAFRYLAQYYFKCKLWDEASTCAQKCCAFNDTREEGKALLRQILQLRNQGETPTTEVPAPFFLPASLSANNTPTRRVSPLNLSSVTP cdc27_human Cell division cycle protein 27 homolog CDC27 D0S1430E D17S978E CDC27 homolog H-NUC ANAPC3 Anaphase-promoting complex subunit 3 protein protein human Homo sapiens Human MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF cdc16_human Cell division cycle protein 16 homolog ANAPC6 CDC16 homolog Anaphase-promoting complex subunit 6 Cyclosome subunit 6 CDC16 protein protein human Homo sapiens Human MNLERLRKRVRQYLDQQQYQSALFWADKVASLSREEPQDIYWLAQCLYLTAQYHRAAHALRSRKLDKLYEACRYLAARCHYAAKEHQQALDVLDMEEPINKRLFEKYLKDESGFKDPSSDWEMSQSSIKSSICLLRGKIYDALDNRTLATYSYKEALKLDVYCFEAFDLLTSHHMLTAQEEKELLESLPLSKLCNEEQELLRFLFENKLKKYNKPSETVIPESVDGLQENLDVVVSLAERHYYNCDFKMCYKLTSVVMEKDPFHASCLPVHIGTLVELNKANELFYLSHKLVDLYPSNPVSWFAVGCYYLMVGHKNEHARRYLSKATTLEKTYGPAWIAYGHSFAVESEHDQAMAAYFTAAQLMKGCHLPMLYIGLEYGLTNNSKLAERFFSQALSIAPEDPFVMHEVGVVAFQNGEWKTAEKWFLDALEKIKAIGNEVTVDKWEPLLNNLGHVCRKLKKYAEALDYHRQALVLIPQNASTYSAIGYIHSLMGNFENAVDYFHTALGLRRDDTFSVTMLGHCIEMYIGDSEAYIGADIKDKLKCYDFDVHTMKTLKNIISPPWDFREFEVEKQTAEETGLTPLETSRKTPDSRPSLEETFEIEMNESDMMLETSMSDHST apc4_human Anaphase-promoting complex subunit 4 APC4 Cyclosome subunit 4 ANAPC4 protein protein human Homo sapiens Human MLRFPTCFPSFRVVGEKQLPQEIIFLVWSPKRDLIALANTAGEVLLHRLASFHRVWSFPPNENTGKEVTCLAWRPDGKLLAFALADTKKIVLCDVEKPESLHSFSVEAPVSCMHWMEVTVESSVLTSFYNAEDESNLLLPKLPTLPKNYSNTSKIFSEENSDEIIKLLGDVRLNILVLGGSSGFIELYAYGMFKIARVTGIAGTCLALCLSSDLKSLSVVTEVSTNGASEVSYFQLETNLLYSFLPEVTRMARKFTHISALLQYINLSLTCMCEAWEEILMQMDSRLTKFVQEKNTTTSVQDEFMHLLLWGKASAELQTLLMNQLTVKGLKKLGQSIESSYSSIQKLVISHLQSGSESLLYHLSELKGMASWKQKYEPLGLDAAGIEEAITAVGSFILKANELLQVIDSSMKNFKAFFRWLYVAMLRMTEDHVLPELNKMTQKDITFVAEFLTEHFNEAPDLYNRKGKYFNVERVGQYLKDEDDDLVSPPNTEGNQWYDFLQNSSHLKESPLLFPYYPRKSLHFVKRRMENIIDQCLQKPADVIGKSMNQAICIPLYRDTRSEDSTRRLFKFPFLWNNKTSNLHYLLFTILEDSLYKMCILRRHTDISQSVSNGLIAIKFGSFTYATTEKVRRSIYSCLDAQFYDDETVTVVLKDTVGREGRDRLLVQLPLSLVYNSEDSAEYQFTGTYSTRLDEQCSAIPTRTMHFEKHWRLLESMKAQYVAGNGFRKVSCVLSSNLRHVRVFEMDIDDEWELDESSDEEEEASNKPVKIKEEVLSESEAENQQAGAAALAPEIVIKVEKLDPELDS q8ixj6-2 NAD-dependent protein deacetylase sirtuin-2 SIRT2 SIR2L SIR2L2 SIR2-like protein 2 Regulatory protein SIR2 homolog 2 protein protein human Homo sapiens Human MDFLRNLFSQTLSLGSQKERLLDELTLEGVARYMQSERCRRVICLVGAGISTSAGIPDFRSPSTGLYDNLEKYHLPYPEAIFEISYFKKHPEPFFALAKELYPGQFKPTICHYFMRLLKDKGLLLRCYTQNIDTLERIAGLEQEDLVEAHGTFYTSHCVSASCRHEYPLSWMKEKIFSEVTPKCEDCQSLVKPDIVFFGESLPARFFSCMQSDFLKVDLLLVMGTSLQVQPFASLISKAPLSTPRLLINKEKAGQSDPFLGMIMGLGGGMDFDSKKAYRDVAWLGECDQGCLALAELLGWKKELEDLVRREHASIDAQSGAGVPNPSTSASPKKSPPPAKDEARTTEREKPQ sirt2-fzr1 1232794 n/a 1232803 unspecified role unspecified role prey prey n/a 1232804 unspecified role unspecified role bait bait physical association physical association false false false Fig 4C fzr1-sirt2 1232794 n/a 1232804 unspecified role unspecified role prey prey n/a 1232803 unspecified role unspecified role bait bait physical association physical association false false false Fig 4D sirt2-cdc20 1232794 n/a 1232804 unspecified role unspecified role bait bait n/a 1232805 unspecified role unspecified role prey prey physical association physical association false false false Fig 4E cdc20-sirt2 1232794 n/a 1232804 unspecified role unspecified role prey prey n/a 1232805 unspecified role unspecified role bait bait physical association physical association false false false Fig 4F fzr1-cdc27-10 1232794 n/a 1232804 unspecified role unspecified role ancillary ancillary n/a 1232806 unspecified role unspecified role prey prey n/a 1232807 unspecified role unspecified role bait bait n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig 5E (LHS) SIRT2−/− MEFs displayed hyperacetylated CDH1 (Figure 5E) and CDC20 (Figure 5F) that was accompanied by decreased interaction with CDC27, and these phenotypes could be reversed by re-expression of SIRT2-WT, but not SIRT2-HY (Figures 5E and 5F). fzr1-cdc27-13 1232794 n/a 1232806 unspecified role unspecified role prey prey n/a 1232807 unspecified role unspecified role bait bait n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1232808 unspecified role unspecified role prey prey Q8IXJ6:p.His187Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain false region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig 5E (RHS) The HY mutation of SIRT2 results in loss of it's catalytic activity (deacetylation). SIRT2−/− MEFs displayed hyperacetylated CDH1 (Figure 5E) and CDC20 (Figure 5F) that was accompanied by decreased interaction with CDC27, and these phenotypes could be reversed by re-expression of SIRT2-WT, but not SIRT2-HY (Figures 5E and 5F). cdc20-fzr1 1232794 n/a 1232806 unspecified role unspecified role prey prey n/a 1232804 unspecified role unspecified role ancillary ancillary n/a 1232809 unspecified role unspecified role bait bait n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig 5F (LHS) SIRT2−/− MEFs displayed hyperacetylated CDH1 (Figure 5E) and CDC20 (Figure 5F) that was accompanied by decreased interaction with CDC27, and these phenotypes could be reversed by re-expression of SIRT2-WT, but not SIRT2-HY (Figures 5E and 5F). cdc20-fzr1-1 1232794 n/a 1232809 unspecified role unspecified role bait bait n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1232806 unspecified role unspecified role prey prey n/a 1232804 unspecified role unspecified role ancillary ancillary region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 5F (RHS) SIRT2−/− MEFs displayed hyperacetylated CDH1 (Figure 5E) and CDC20 (Figure 5F) that was accompanied by decreased interaction with CDC27, and these phenotypes could be reversed by re-expression of SIRT2-WT, but not SIRT2-HY (Figures 5E and 5F). sirt2-anapc1-2 1232795 n/a 1232807 unspecified role unspecified role prey prey n/a 1232809 unspecified role unspecified role prey prey n/a 1232810 unspecified role unspecified role prey prey n/a 1232804 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1232811 unspecified role unspecified role prey prey n/a 1232812 unspecified role unspecified role prey prey n/a 1232813 unspecified role unspecified role prey prey n/a 1232814 unspecified role unspecified role prey prey n/a 1232815 unspecified role unspecified role prey prey association association false false false Fig 4B fzr1-cdc27-6 1232795 n/a 1232808 unspecified role unspecified role ancillary ancillary region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q8IXJ6:p.His187Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain false n/a 1232807 unspecified role unspecified role bait bait n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1232813 unspecified role unspecified role prey prey association association false false false Fig 5A (top) The HY mutation of SIRT2 results in loss of it's catalytic activity (deacetylation). Expression of SIRT2-WT, but not SIRT2-HY, reduced the acetylated form of CDH1 (Figure 5A) and CDC20 (Figure 5B), and increased their interaction with CDC27. cdc20-cdc27 1232795 n/a 1232813 unspecified role unspecified role prey prey n/a 1232808 unspecified role unspecified role ancillary ancillary Q8IXJ6:p.His187Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain false region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1232809 unspecified role unspecified role bait bait n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false association association false false false Fig 5B (top) The HY mutation of SIRT2 results in loss of it's catalytic activity (deacetylation). Expression of SIRT2-WT, but not SIRT2-HY, reduced the acetylated form of CDH1 (Figure 5A) and CDC20 (Figure 5B), and increased their interaction with CDC27. fzr1-cdc27-7 1232795 n/a 1232813 unspecified role unspecified role prey prey P30260:p.Cys66Arg mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false n/a 1232807 unspecified role unspecified role bait bait n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q9UM11:p.Lys69Arg mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false Q9UM11:p.Lys159Arg mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 5C, 6A. siRNA-mediated knockdown of SIRT2 increased acetylation of CDH1 and CDC20 and decreased their interaction with CDC27. Mutations of the acetylation sites, K69R and K159R of CDH1 (Figure 6A), and K66R of CDC20 (Figure 6B), reduced the acetylation of these proteins and enhanced their interaction with CDC27. cdc20-cdc27-1 1232795 n/a 1232809 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1232813 unspecified role unspecified role prey prey physical association physical association false false false Fig 5D, 6B. siRNA-mediated knockdown of SIRT2 increased acetylation of CDH1 and CDC20 and decreased their interaction with CDC27. Mutations of the acetylation sites, K69R and K159R of CDH1 (Figure 6A), and K66R of CDC20 (Figure 6B), reduced the acetylation of these proteins and enhanced their interaction with CDC27. cdc27-fzr1-1 1232796 n/a 1232808 unspecified role unspecified role ancillary ancillary Q8IXJ6:p.His187Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain false region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1232807 unspecified role unspecified role prey prey n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1232813 unspecified role unspecified role bait bait physical association physical association false false false Fig 5A (bottom) The HY mutation of SIRT2 results in loss of it's catalytic activity (deacetylation). Expression of SIRT2-WT, but not SIRT2-HY, reduced the acetylated form of CDH1 (Figure 5A) and CDC20 (Figure 5B), and increased their interaction with CDC27. cdc27-cdc20-1 1232796 n/a 1232808 unspecified role unspecified role ancillary ancillary Q8IXJ6:p.His187Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain false region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1232813 unspecified role unspecified role bait bait n/a 1232809 unspecified role unspecified role prey prey n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig 5B (bottom) The HY mutation of SIRT2 results in loss of it's catalytic activity (deacetylation). Expression of SIRT2-WT, but not SIRT2-HY, reduced the acetylated form of CDH1 (Figure 5A) and CDC20 (Figure 5B), and increased their interaction with CDC27. cdc27-cdc20-2 1232796 n/a 1232809 unspecified role unspecified role prey prey n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1232813 unspecified role unspecified role bait bait physical association physical association false false false Fig 5D siRNA-mediated knockdown of SIRT2 increased acetylation of CDH1 and CDC20 and decreased their interaction with CDC27. fzr1-sirt2-3 1232797 n/a 1232807 enzyme target enzyme target substrate substrate neutral component neutral component region ac1res monoacetylated residue Acetyl Acetylation (N terminus, N epsilon of Lysine, O of Serine) (Ac) Acetylation Ac1Res undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 n/a 1232816 enzyme enzyme neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q8IXJ6-2:p.His150Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false Fig S4A (LHS) The HY mutation of SIRT2 results in loss of it's catalytic activity (deacetylation). SIRT2-WT, but not SIRT2-HY was able to deacetylate both proteins - CDH1 and CDC20. cdc20-sirt2-1 1232797 n/a 1232809 enzyme target enzyme target substrate substrate neutral component neutral component region ac1res monoacetylated residue Acetyl Acetylation (N terminus, N epsilon of Lysine, O of Serine) (Ac) Acetylation Ac1Res undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 n/a 1232816 enzyme enzyme neutral component neutral component Q8IXJ6-2:p.His150Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain false region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false Fig S4A (RHS) The HY mutation of SIRT2 results in loss of it's catalytic activity (deacetylation). SIRT2-WT, but not SIRT2-HY was able to deacetylate both proteins - CDH1 and CDC20. cdc20-sirt2-2 1232798 n/a 1232809 enzyme target enzyme target substrate substrate neutral component neutral component n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region ac1res monoacetylated residue Acetyl Acetylation (N terminus, N epsilon of Lysine, O of Serine) (Ac) Acetylation Ac1Res undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1232816 enzyme enzyme neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q8IXJ6-2:p.His150Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig S4C The HY mutation of SIRT2 results in loss of it's catalytic activity (deacetylation). SIRT2-WT, but not SIRT2-HY was able to deacetylate both proteins - CDH1 and CDC20. fzr1-sirt2-4 1232798 n/a 1232807 enzyme target enzyme target substrate substrate neutral component neutral component n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region ac1res monoacetylated residue Acetyl Acetylation (N terminus, N epsilon of Lysine, O of Serine) (Ac) Acetylation Ac1Res undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1232816 enzyme enzyme neutral component neutral component Q8IXJ6-2:p.His150Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain false region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig S4B The HY mutation of SIRT2 results in loss of it's catalytic activity (deacetylation). SIRT2-WT, but not SIRT2-HY was able to deacetylate both proteins - CDH1 and CDC20. sirt2-aurka 1232799 n/a 1232810 unspecified role unspecified role prey prey n/a 1232804 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 3B aurka-sirt2 1232800 n/a 1232810 unspecified role unspecified role bait bait n/a 1232804 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 3B sirt2-anapc1 1232801 n/a 1232811 unspecified role unspecified role prey prey n/a 1232813 unspecified role unspecified role prey prey n/a 1232814 unspecified role unspecified role prey prey n/a 1232815 unspecified role unspecified role prey prey n/a 1232812 unspecified role unspecified role prey prey n/a 1232804 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1232810 unspecified role unspecified role prey prey association association false false false Fig 4A aurka-aurka-1 1232802 n/a 1232810 unspecified role unspecified role neutral component neutral component n/a 1232804 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Fig S3C