IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ chappie-2011-3 A pseudoatomic model of the dynamin polymer identifies a hydrolysis-dependent powerstroke. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Chappie J.S., Mears J.A., Fang S., Leonard M., Schmid S.L., Milligan R.A., Hinshaw J.E., Dyda F. jennyh@helix.nih.gov, dyda@helix.nih.gov Cell (0092-8674) 2011 Only protein-protein interactions imex curation chappie-2011-5 A pseudoatomic model of the dynamin polymer identifies a hydrolysis-dependent powerstroke. in vitro In vitro crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Chappie J.S., Mears J.A., Fang S., Leonard M., Schmid S.L., Milligan R.A., Hinshaw J.E., Dyda F. Targeted crosslinking of the engineered cysteine residues in the N-terminal GTPase and C-terminal GED regions by 1,1-methanediyl bismethanethiosulfonate (MTS-1-MTS) produced a dimer, the less specific crosslinking of surface-reactive amines by BS3 primarily yields a tetramer. jennyh@helix.nih.gov, dyda@helix.nih.gov Cell (0092-8674) 2011 Only protein-protein interactions imex curation chappie-2011-1 A pseudoatomic model of the dynamin polymer identifies a hydrolysis-dependent powerstroke. in vitro In vitro electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy predetermined predetermined participant predetermined Chappie J.S., Mears J.A., Fang S., Leonard M., Schmid S.L., Milligan R.A., Hinshaw J.E., Dyda F. jennyh@helix.nih.gov, dyda@helix.nih.gov Cell (0092-8674) 2011 Only protein-protein interactions imex curation chappie-2011-2 A pseudoatomic model of the dynamin polymer identifies a hydrolysis-dependent powerstroke. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Chappie J.S., Mears J.A., Fang S., Leonard M., Schmid S.L., Milligan R.A., Hinshaw J.E., Dyda F. jennyh@helix.nih.gov, dyda@helix.nih.gov Cell (0092-8674) 2011 Only protein-protein interactions imex curation chappie-2011-4 A pseudoatomic model of the dynamin polymer identifies a hydrolysis-dependent powerstroke. in vitro In vitro density sedimentation cosedimentation through density gradient density sedimentation predetermined predetermined participant predetermined Chappie J.S., Mears J.A., Fang S., Leonard M., Schmid S.L., Milligan R.A., Hinshaw J.E., Dyda F. jennyh@helix.nih.gov, dyda@helix.nih.gov Cell (0092-8674) 2011 Only protein-protein interactions imex curation chappie-2011-6 A pseudoatomic model of the dynamin polymer identifies a hydrolysis-dependent powerstroke. in vitro In vitro gtpase assay gtpase assay GTPase gtp hydrolisis gtp hydrolisis GTPase predetermined predetermined participant predetermined Chappie J.S., Mears J.A., Fang S., Leonard M., Schmid S.L., Milligan R.A., Hinshaw J.E., Dyda F. jennyh@helix.nih.gov, dyda@helix.nih.gov Cell (0092-8674) 2011 Only protein-protein interactions imex curation dyn1_human Dynamin-1 DNM1 DNM protein protein human Homo sapiens Human MGNRGMEDLIPLVNRLQDAFSAIGQNADLDLPQIAVVGGQSAGKSSVLENFVGRDFLPRGSGIVTRRPLVLQLVNATTEYAEFLHCKGKKFTDFEEVRLEIEAETDRVTGTNKGISPVPINLRVYSPHVLNLTLVDLPGMTKVPVGDQPPDIEFQIRDMLMQFVTKENCLILAVSPANSDLANSDALKVAKEVDPQGQRTIGVITKLDLMDEGTDARDVLENKLLPLRRGYIGVVNRSQKDIDGKKDITAALAAERKFFLSHPSYRHLADRMGTPYLQKVLNQQLTNHIRDTLPGLRNKLQSQLLSIEKEVEEYKNFRPDDPARKTKALLQMVQQFAVDFEKRIEGSGDQIDTYELSGGARINRIFHERFPFELVKMEFDEKELRREISYAIKNIHGIRTGLFTPDMAFETIVKKQVKKIREPCLKCVDMVISELISTVRQCTKKLQQYPRLREEMERIVTTHIREREGRTKEQVMLLIDIELAYMNTNHEDFIGFANAQQRSNQMNKKKTSGNQDEILVIRKGWLTINNIGIMKGGSKEYWFVLTAENLSWYKDDEEKEKKYMLSVDNLKLRDVEKGFMSSKHIFALFNTEQRNVYKDYRQLELACETQEEVDSWKASFLRAGVYPERVGDKEKASETEENGSDSFMHSMDPQLERQVETIRNLVDSYMAIVNKTVRDLMPKTIMHLMINNTKEFIFSELLANLYSCGDQNTLMEESAEQAQRRDEMLRMYHALKEALSIIGDINTTTVSTPMPPPVDDSWLQVQSVPAGRRSPTSSPTPQRRAPAVPPARPGSRGPAPGPPPAGSALGGAPPVPSRPGASPDPFGPPPQVPSRPNRAPPGVPSRSGQASPSRPESPRPPFDL gdp-tetrafluoroaluminate transition-state analogue GDP.AlF4 small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) GDP.AlF4 is a transition stsate analogue gcp guanosine 5'-[beta,gamma-methylene]triphosphate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) gtp guanosine triphosphate - GTP 5'-GTP GTP Guanosine 5'-triphosphate guanosine 5'-triphosphoric acid guanosine triphosphate GUANOSINE-5'-TRIPHOSPHATE H4gtp guanosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2 dnm1-gdp_tetrafluoro 1232021 n/a 1232027 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. Stoichiometry: 2.0 n/a 1232028 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false Supp. Fig. 4A dnm1-3 1232021 n/a 1232027 unspecified role unspecified role neutral component neutral component Q05193:p.[Arg15Cys;Cys86Ser;Cys424Ser;Cys427Ser;Cys442Ser;Cys607Ser;Cys708Ser;Arg730Cys] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. Stoichiometry: 4.0 direct interaction direct interaction false false false 1232021 Supp. Fig. 5D, 5E Cross-linking with 1,1-methanediyl bismethanethiosulfonate (MTS-1-MTS) also produced a tetramer. dnm1-4 1232021 n/a 1232027 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection Stoichiometry: 4.0 direct interaction direct interaction false false false 1232021 Supp. Fig. 5C dnm1 1232022 n/a 1232027 unspecified role unspecified role neutral component neutral component Q05193:p.[Arg15Cys;Cys86Ser;Cys424Ser;Cys427Ser;Cys442Ser;Cys607Ser;Cys708Ser;Arg730Cys] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 6B dnm1-1 1232022 n/a 1232027 unspecified role unspecified role neutral component neutral component Q05193:p.[Arg15Cys;Cys86Ser;Cys424Ser;Cys427Ser;Cys442Ser;Cys607Ser;Cys708Ser;Arg730Cys] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. Stoichiometry: 4.0 direct interaction direct interaction false false false Fig. 6B dyn1-1 1232023 n/a 1232027 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. n/a 1232027 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 1232053 1232050 direct interaction direct interaction false false false Fig. 1 dnm1-gcp-4 1232024 n/a 1232027 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. Stoichiometry: 2.0 n/a 1232029 unspecified role unspecified role neutral component neutral component trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. Stoichiometry: 2.0 direct interaction direct interaction false false false Fig 3, Table S2. 2.2A Rworking = 22.53% Rfree = 26.91% dnm1-gcp-3 1232025 n/a 1232027 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. Stoichiometry: 2.0 n/a 1232028 unspecified role unspecified role unspecified role unspecified role direct interaction direct interaction false false false 1232025 Supp. Fig. 4B dnm1-gtp-1 1232026 n/a 1232030 enzyme target enzyme target substrate substrate neutral component neutral component n/a 1232027 enzyme enzyme neutral component neutral component Q05193:p.[Arg15Cys;Cys86Ser;Cys424Ser;Cys427Ser;Cys442Ser;Cys607Ser;Cys708Ser;Arg730Cys] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction false false false Fig. S5 A/B DynRCL R15C/R730C reconstitutes WT dynamin's basal GTPase activity (A) and is stimulated by self-assembly in low ionic strength (B).