IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ whorton-2011-1 Crystal Structure of the Mammalian GIRK2 K(+) Channel and Gating Regulation by G Proteins, PIP(2), and Sodium. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Whorton M.R., Mackinnon R. mackinn@rockefeller.edu Cell (0092-8674) 2011 Only protein-protein interactions imex curation kcnj6_mouse G protein-activated inward rectifier potassium channel 2 Kcnj6 Girk2 Kcnj7 W Inward rectifier K(+) channel Kir3.2 Potassium channel, inwardly rectifying subfamily J member 6 protein protein mouse Mus musculus Mouse MTMAKLTESMTNVLEGDSMDQDVESPVAIHQPKLPKQARDDLPRHISRDRTKRKIQRYVRKDGKCNVHHGNVRETYRYLTDIFTTLVDLKWRFNLLIFVMVYTVTWLFFGMIWWLIAYIRGDMDHIEDPSWTPCVTNLNGFVSAFLFSIETETTIGYGYRVITDKCPEGIILLLIQSVLGSIVNAFMVGCMFVKISQPKKRAETLVFSTHAVISMRDGKLCLMFRVGDLRNSHIVEASIRAKLIKSKQTSEGEFIPLNQSDINVGYYTGDDRLFLVSPLIISHEINQQSPFWEISKAQLPKEELEIVVILEGIVEATGMTCQARSSYITSEILWGYRFTPVLTMEDGFYEVDYNSFHETYETSTPSLSAKELAELANRAEVPLSWSVSSKLNQHAELETEEEEKNPEELTERNGDVANLENESKV pip2-octanoylglyceryl L-ALPHA-D-MYOPHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE, D(+)SN1,2DI-O-OCTANOYLGLYCERYL small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) kcnj6-1 1231970 n/a 1231971 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 4.0 direct interaction direct interaction false false false Supp. Table 1 3.5A Rworking = 26% Rfree = 27.27% An Arg201ala mutant also crystallised as a tetramer (3SYC) kcnj6-c8-pip2 1231970 n/a 1231972 unspecified role unspecified role neutral component neutral component Stoichiometry: 1.0 n/a 1231971 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 4.0 direct interaction direct interaction false false false Supp. Table 1 3.0A Rworking = 24% Rfree = 26.92% G proteins open a G loop gate in the cytoplasmic domain in the presence of PIP2. An Arg201ala mutant also crystallised as a tetramer (3SYQ). This shows a large change in both the G loop and the inner helix gates in comparison to wild tye and only two of the four subunits are free to bind PIP2 and undergo the conformational change