IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ nakata-2011-1 Assembly Mechanism of FCT Region Type 1 Pili in Serotype M6 Streptococcus pyogenes. strp6 Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394) comigration in sds comigration in sds page comigration in sds western blot western blot Immuno blot Immuno blot Nakata M., Kimura K.R., Sumitomo T., Wada S., Sugauchi A., Oiki E., Higashino M., Kreikemeyer B., Podbielski A., Okahashi N., Hamada S., Isoda R., Terao Y., Kawabata S. The K175A mutation given in this paper for the T6 molecule (as found in the VAKS sequence) is K163A in UniProt entry P18481 (S.Pyogenes M6). kawabata@dent.osaka-u.ac.jp J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation nakata-2011-2 Assembly Mechanism of FCT Region Type 1 Pili in Serotype M6 Streptococcus pyogenes. strp6 Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394) anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Nakata M., Kimura K.R., Sumitomo T., Wada S., Sugauchi A., Oiki E., Higashino M., Kreikemeyer B., Podbielski A., Okahashi N., Hamada S., Isoda R., Terao Y., Kawabata S. kawabata@dent.osaka-u.ac.jp J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation tee6_strp6 Trypsin-resistant surface T6 protein tee6 T6 antigen M6_Spy0160 protein protein strp6 Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394) MLACLAILAVVGLGMTRVSALSKDDTAQLKITNIEGGPTVTLYKIGEGVYNTNGDSFINFKYAEGVSLTETGPTSQEITTIANGINTGKIKPFSTENVSISNGTATYNARGASVYIALLTGATDGRTYNPILLAASYNGEGNLVTKNIDSKSNYLYGQTSVAKSSLPSITKKVTGTIDDVNKKTTSLGSVLSYSLTFELPSYTKEAVNKTVYVSDNMSEGLTFNFNSLTVEWKGKMANITEDGSVMVENTKIGIAKEVNNGFNLSFIYDSLESISPNISYKAVVNNKAIVGEEGNPNKAEFFYSNNPTKGNTYDNLDKKPDKGNGITSKEDSKIVYTYQIAFRKVDSVSKTPLIGAIFGVYDTSNKLIDIVTTNKNGYAISTQVSSGKYKIKELKAPKGYSLNTETYEITANWVTATVKTSANSKSTTYTSDKNKATDNSEQVGWLKNGIFYSIDSRPTGNDVKEAYIESTKALTDGTTFSKSNEGSGTVLLETDIPNTKLGELPSTGSIGTYLFKAIGSAAMIGAIGIYIVKRRKA q5xe69_strp6 M6_Spy0159 protein protein strp6 Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394) MYSRLKRELVIVINRKKKYKLIRLMVTVGLIFSQLVLPIRRLGLQMISTQTKVIPQEIVTQTETQGTQVVATKQKLESENSSLKVALKRESGFEHNATIDASLDTESQGDNSQRSVTQAIVTMALELRKQGLSIVDTKIVRIQSSTNQRNDITTTLTFKNGLSLEGASTEANDPNVRVGIVNPNDTVQTITPTIKQDADGKVKNLVFTGRLGKQVIIVSTTRLKEEQTISLDSYGELVIDGAVGLSQKDRPPYSKPITVNILKPKLSSIESSLDSKDFEIVKTIDNLYTWDDQFYLLDFISKQYEVLKTDYQSAKDSTPQTRDILFGEYTVEPLVMNKGHNNTINIYIRSTRPLGLKPIGAAPALIQPRSFRSLTPRSTRMKRSAPVEKFEGELEHHKRIDYLGDNQNNPDTTIDDKEDEHDTSDLYRLYLDMTGKKNPLDILVVVDKSGSMQEGIGSVQRYRYYAQRWDDYYSQWVYHGTFDYSSYQGESFNRGQIHYRYRGIVSVSDGIRRDDAVKNSLLGVNGLLQRFVNINPENKLSVIGFQGSADYHAGKWYPDQSPRGGFYQPNLNNSRDAELLKGWSTNSLLDPNTLTALHNNGTNYHAALLKAKEILNEVKDDGRRKIMIFISDGVPTFYFGEDGYRSGNGSSNDRNNVTRSQEGSKLAIDEFKARYPNLSIYSLGVSKDINSDTASSSPVVLKYLSGEEHYYGITDTAELEKTLNKIVEDSKLSQLGISDSLSQYVDYYDKQPDVLVTRKSKVNDETEILYQKDQVQEAGKDIIDKVVFTPKTTSQPKGKVTLTFKSDYKVDDEYTYTLSFNVKASDEAYEKYKDNEGRYSEMGDSDTDYGTNQTSSGKGGLPSNSDASVNYMADGREQKLPYKHPVIQVKTVPITFTKVDADNNQKKLAGVEFELRKEDKKIVWEKGTTGSNGQLNFKYLQKGKTYYLYETKAKLGYTLPENPWEVAVANNGDIKVKHPIEGELKSKDGSYMIKNYKIYQLPSSGGRGSQIFIIVGSMTATVALLFYRRQHRKKQY tee6-tee6 1240772 n/a 1240774 unspecified role unspecified role neutral component neutral component lpstg region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false P18481:p.Lys163Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P18481:p.Thr507Ser mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false n/a 1240774 unspecified role unspecified role neutral component neutral component P18481:p.Thr507Ser mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false lpstg region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false P18481:p.Lys163Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 2, 3, 4, 5, 6, Supp. Fig. 4 q5xe69-tee6 1240772 n/a 1240774 unspecified role unspecified role neutral component neutral component P18481:p.Thr507Ser mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P18481:p.Lys163Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 n/a 1240775 unspecified role unspecified role neutral component neutral component pss region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Q5XE69:p.Ser1004Thr mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 physical association physical association false false false Fig. 2, 3, 4, 5, 6, Supp. Fig. 5 q5xe69-tee6-2 1240773 n/a 1240774 unspecified role unspecified role prey prey Stoichiometry: 1.0 n/a 1240775 unspecified role unspecified role bait bait Stoichiometry: 1.0 physical association physical association false false false Supp. Fig. 3