IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ weinberg-2011-1 The inside-out mechanism of dicers from budding yeasts. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Weinberg D.E., Nakanishi K., Patel D.J., Bartel D.P. pateld@mskcc.org, dbartel@wi.mit.edu Cell (0092-8674) 2011 Only protein-protein interactions imex curation weinberg-2011-2 The inside-out mechanism of dicers from budding yeasts. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Weinberg D.E., Nakanishi K., Patel D.J., Bartel D.P. pateld@mskcc.org, dbartel@wi.mit.edu Cell (0092-8674) 2011 Only protein-protein interactions imex curation weinberg-2011-3 The inside-out mechanism of dicers from budding yeasts. in vitro In vitro light scattering light scattering predetermined predetermined participant predetermined Weinberg D.E., Nakanishi K., Patel D.J., Bartel D.P. pateld@mskcc.org, dbartel@wi.mit.edu Cell (0092-8674) 2011 Only protein-protein interactions imex curation weinberg-2011-4 The inside-out mechanism of dicers from budding yeasts. in vitro In vitro crosslink cross-linking study crosslink weight silver stain molecular weight estimation by silver staining weight silver stain Weinberg D.E., Nakanishi K., Patel D.J., Bartel D.P. Cross-linking agent - glutaraldehyde pateld@mskcc.org, dbartel@wi.mit.edu Cell (0092-8674) 2011 Only protein-protein interactions imex curation a7tr32_vanpo Kpol_455p11 protein protein vanpo Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) MSDQVYVPPTSAPMSNELKVREFYRLHNACVKLKESIKLIYENPLVTDQNVLNLGTAENTIDYTILNTPTLNVAKTLLGNRYSLDLIDLFQSHDFKDSNTDVDMFIKYPVVYDENLENLAFMHKSFPNMNAHLNDAQKTQLSNERLEFLGDSWLGALVSYIVYTRFPSANEGMLSQMKESIVNNNNLFDWSTKLNFTKRLQGNIATPTRVVKDKMSKRYADCVEAYIGALVIDRFGTEFLDIKEWLEELSEKKLAKSSQMVIKEPLNKNAKNELAELLQINKLGHKLHYRKLTEMPPFRVEVKIGDILLDEAEGNSIREAEHRAAMKVLENDELLRKYSVYDLEDGNSKKYSIEETDLKHDIESSGISNENFLQNEGNGTENIESKFKQLNISNDSNKDDLVNNITNSVFSKLQDVVSKIVIDAISESIPNNPHKIEQSRNGKVGTQTNTSATTSDVLSVQTSIPKSESTSADKAPITCDPNAKQALYTLLSRSQVFPDYEMVQHSMDNFTANCIIKGSGIFLASGRGRSKKIAQQNAASAALRSPILKSILEEQNNF dcr1-dcr1 1173578 n/a 1173582 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false 2.29A Working 17.74%, Free:21.97% Figure 2A, Table S2 a7tr32 1173578 n/a 1173582 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false A7TR32:p.Glu224Gln mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false 1.98A Working 19.65%, Free:23.59% Figure 2C, Table S2 dcr1-dcr1-1 1173579 n/a 1173582 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Supp. Figure 2A dcr1-dcr1-2 1173580 n/a 1173582 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Supp. Figure 2B dcr1-dcr1-3 1173581 n/a 1173582 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 4F, Supp. Figures 2C, 2F The coordinates of the delta N construct have not been given in the publication so have been inferred from Fig. 1A and InterPro annotation.