IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ wang-2011a-1 A hormone-dependent module regulating energy balance. in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Wang B., Moya N., Niessen S., Hoover H., Mihaylova M.M., Shaw R.J., Yates J.R., Fischer W.H., Thomas J.B., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis jthomas@salk.edu, montminy@salk.edu Cell (0092-8674) 2011 Only protein-protein interactions imex curation wang-2011a-2 A hormone-dependent module regulating energy balance. drome Drosophila melanogaster (Fruit fly) Fruit fly anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang B., Moya N., Niessen S., Hoover H., Mihaylova M.M., Shaw R.J., Yates J.R., Fischer W.H., Thomas J.B., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis jthomas@salk.edu, montminy@salk.edu Cell (0092-8674) 2011 Only protein-protein interactions imex curation wang-2011a-3 A hormone-dependent module regulating energy balance. in vitro In vitro deacetylase assay deacetylase assay western blot western blot Immuno blot Immuno blot Wang B., Moya N., Niessen S., Hoover H., Mihaylova M.M., Shaw R.J., Yates J.R., Fischer W.H., Thomas J.B., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis jthomas@salk.edu, montminy@salk.edu Cell (0092-8674) 2011 Only protein-protein interactions imex curation wang-2011a-4 A hormone-dependent module regulating energy balance. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang B., Moya N., Niessen S., Hoover H., Mihaylova M.M., Shaw R.J., Yates J.R., Fischer W.H., Thomas J.B., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis jthomas@salk.edu, montminy@salk.edu Cell (0092-8674) 2011 Only protein-protein interactions imex curation wang-2011a-5 A hormone-dependent module regulating energy balance. in vitro In vitro acetylase assay acetylase assay western blot western blot Immuno blot Immuno blot Wang B., Moya N., Niessen S., Hoover H., Mihaylova M.M., Shaw R.J., Yates J.R., Fischer W.H., Thomas J.B., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis jthomas@salk.edu, montminy@salk.edu Cell (0092-8674) 2011 Only protein-protein interactions imex curation wang-2011a-6 A hormone-dependent module regulating energy balance. drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip fingerprinting peptide massfingerprinting fingerprinting Wang B., Moya N., Niessen S., Hoover H., Mihaylova M.M., Shaw R.J., Yates J.R., Fischer W.H., Thomas J.B., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis jthomas@salk.edu, montminy@salk.edu Cell (0092-8674) 2011 Only protein-protein interactions imex curation wang-2011a-7 A hormone-dependent module regulating energy balance. mouse-hepatocyte Mouse hepatocyte hepatocyte Liver primary cell A hepatocyte is a cell of the main tissue of the liver. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang B., Moya N., Niessen S., Hoover H., Mihaylova M.M., Shaw R.J., Yates J.R., Fischer W.H., Thomas J.B., Montminy M. Apoptosis - Interactions involving proteins with a function related to apoptosis jthomas@salk.edu, montminy@salk.edu Cell (0092-8674) 2011 Only protein-protein interactions imex curation q4qqa7_drome Sik3 CG15072 CG18604 Dmel\CG42856 EMK KIAA0999 SIK SIK3 CG42856 Dmel_CG42856 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MATTPTAGPAAAPPTSSTPQNYKVPSTSKISVDKLLRVGYYELEKTIGKGNFAVVKLATNIVTKTKVAIKIIDKTCLNEEYLNKTFREIAILKSLRHPHITRLYEVMESQSMIYLVTEYAPNGEIFDHLVANGRMKEPEAARVFTQLVSAVHYCHRRGVVHRDLKAENVLLDKDMNIKLADFGFSNHYEEGATLKTWCGSPPYAAPEVFQGLEYDGPKSDIWSLGVVLYALVCGALPFDGKTILELKSRVVLGKFRIPFFMSQECEQLIRNMLVVEPDRRYTIKQIIKHRWLSEWQSEMQEQERFGDMSPGSGTVSKSASTSSLGSASDSPPQLDSVVMTHMLQLPGLTADMIAQSVHEQRFDNIYAIYNLLHDKLQQRRRENQRLQHHASLAYSRSRKTSITTGVVDRSEPVKQESLDRLSPLSNANASSSALGFGWSDVAVDLEKYGDFELECLARSNDPPVNAQHLSAHAGGGVNGANTRRHTVGPGDVAHEQALANPNVPPIDFKCPPQCSDPTQPVPYYPVNLPMLQNQPLHNLTIKDQHLLKPPVVMGASSFGRRASDGGANLHIYYPATGTVVGPAQGQQMDTAGYYINPNCGTDPLAVQELSPLNEQSVAQMQCCQENATGECNEELQSYMQKRGGTQRHTVGCTEDLSVAHGSGAGSQSQAPTTSSNMRQRRTGLLTVTERPPGALERLTKIL akt1_drome RAC serine/threonine-protein kinase Akt1 CG4006 Akt Protein kinase B protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MNYLPFVLQRRSTVVASAPAPGSASRIPESPTTTGSNIINIIYSQSTHPNSSPTSGSAEKFSWQQSWPSRTSAAPTHDSGTMSINTTFDLSSPSVTSGHALTEQTQVVKEGWLMKRGEHIKNWRQRYFVLHSDGRLMGYRSKPADSASTPSDFLLNNFTVRGCQIMTVDRPKPFTFIIRGLQWTTVIERTFAVESELERQQWTEAIRNVSSRLIDVGEVAMTPSEQTDMTDVDMATIAEDELSEQFSVQGTTCNSSGVKKVTLENFEFLKVLGKGTFGKVILCREKATAKLYAIKILKKEVIIQKDEVAHTLTESRVLKSTNHPFLISLKYSFQTNDRLCFVMQYVNGGELFWHLSHERIFTEDRTRFYGAEIISALGYLHSQGIIYRDLKLENLLLDKDGHIKVADFGLCKEDITYGRTTKTFCGTPEYLAPEVLDDNDYGQAVDWWGTGVVMYEMICGRLPFYNRDHDVLFTLILVEEVKFPRNITDEAKNLLAGLLAKDPKKRLGGGKDDVKEIQAHPFFASINWTDLVLKKIPPPFKPQVTSDTDTRYFDKEFTGESVELTPPDPTGPLGSIAEEPLFPQFSYQGDMASTLGTSSHISTSTSLASMQ q8i9j6_drome Histone deacetylase HDAC4 HDAC4b CG1770 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSSPDDRIPIHDLPSEAGSDERLLHITPATLTLDFKPHPAVDIDQQIMELKKSQELQKQRLINSFQEQSKQMELEHKLQLEHKYQFAVNSHGAFQELRNESMVTAAAAAVAQEQHRQQLHQQQQQHQQQQQQQQQHQQQQQQQQQQARGRDGMKLKQNCSANASPEVKQILNCFILSRKSQAAASNGTTTTSPYRNRGVVKSSSGESLPAGTVTSAHPYKIPQPPPSLLKYESDFPLRKTASEPNLLKIRLKQSVIERKARIGGPAGARRHERLLQAAQRRQQKNSVLTNCNSTPDSGPNSPPSAAALAVGVVGSRGSPTSAPIQEENEEGSQYQPGQRSSINDLPLFSSPSLPNISLGRPHLPNSAQAHAQVNAQVAAQAQAQAQAQAQAHAMFAALAAAQGGCGQPGYYNPLGMAFVGRQPAPLAMIPATGIAPQQPSPVVRSASATSTSSSQASLVGDVAPPQAHAASTILPSSSSYMQQLGSVAGSGVNLHAAAVAAAAAAAAAAGSLPPTNSHGHGHGSHAHPHPHAHGHGHGHGHGIYAGHQHNVPITDAQVAQVHLHKQGHRPLGRTQSAPLPLGHPMLTGAVQLNVVQTHYENSEAERQAYEHQVVNQKVRQTVLTRSGAAAAAAAAAGVSVVREAQLKEEDDDSAAEVMDLTDKKKPPKTVLTSTIATSTSQNLPEALAAAAAAAAYRAPHNASSNSASATKSGIKLRDQEYLQQQREQLLLLQQEEELAKSLMRPLSRTLSSPLVPLGPHGLSQIPDTGQQPAPIATSSSADHIPPVNLSLPHRQHRQLMSTLYASQLRNHQPSASGSPPHKVTTGLAYDPLMLKHSCICGDNAQHPEHSGRLQSVWARLNETDLVKRCDRLRARKATQEELQTVHTEAHAMLFGSNQCQLSRPKLENTLSASFVRLSCGGLGVDLDTTWNEHHTATAARMAAGCVIDLALKTAKGDLRNGFAVVRPPGHHAEANLAMGFCFFNSIAIAAKLLRQRMPEVRRILIVDWDVHHGNGTQQAFYQSPDILYLSIHRHDDGNFFPGTGGPTECGSGAGLGFNVNISWSGALNPPLGDAEYIAAFRTVVMPIARSFNPDIVLVSSGFDAATGHPAPLGGYHVSPACFGFMTRELLQLANGKVVLALEGGYDLAAICDSAQECVRALLGDPAAPIAKAELERPPCQNAINTLQKTIAIQQTHWPCVRMLEHTVGLSALETLKVEHDESETINAMAGLSMQSMHRTLSRDDSEEPMDQDETK foxo_drome Forkhead box protein O foxo Afx Protein FKHR CG3143 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MMDGYAQEWPRLTHTDNGLAMDQLGGDLPLDVGFEPQTRARSNTWPCPRPENFVEPTDELDSTKASNQQLAPGDSQQAIQNANAAKKNSSRRNAWGNLSYADLITHAIGSATDKRLTLSQIYEWMVQNVPYFKDKGDSNSSAGWKNSIRHNLSLHNRFMRVQNEGTGKSSWWMLNPEAKPGKSVRRRAASMETSRYEKRRGRAKKRVEALRQAGVVGLNDATPSPSSSVSEGLDHFPESPLHSGGGFQLSPDFRQRASSNASSCGRLSPIRAQDLEPDWGFPVDYQNTTMTQAHAQALEELTGTMADELTLCNQQQQGFSAASGLPSQPPPPPYQPPQHQQAQQQQQQQSPYALNGPASGYNTLQPQSQCLLHRSLNCSCMHNARDGLSPNSVTTTMSPAYPNSEPSSDSLNTYSNVVLDGPADTAALMVQQQQQQQQQQQLSASLEGQCLEVLNNEAQPIDEFNLENFPVGNLECNVEELLQQEMSYGGLLDINIPLATVNTNLVNSSSGPLSISNISNLSNISSNSGSSLSLNQLQAQLQQQQQQQQAQQQQQAQQQQQQHQQHQQQLLLNNNNNSSSSLELATQTATTNLNARVQYSQPSVVTSPPSWVH ebi_drome F-box-like/WD repeat-containing protein ebi ebi CG4063 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSFSSDEVNFLVYRYLQESGFLHSAYVFGIESHISQSNINGALVPPAALLTILQKGLLYTEVEWSVGEDGEVARPIEGLSLIDAVMPEVKPLKPIVKTEPGKPGAVDSSAPAGGNQNNNAKPEIKIEPGTGVAGSAGGNKIAGSTTGTSTPTDQSASEVDSSGNAANNAGGTYAGNNGAGGNQASTGGSNSTSTPAGGDLAAPGASQKKSQNSNEAGSSSSGNAGNANATSTDDAASSTSTNGNSSTSSSVEQPTSGLTPAGGTVSTSNPDAAASGGASTATGSKAPSGAVTIRVGAQGNNVQSGSSNAQSSAPSGTISSSTSGGAGTPAALVPMDIDENIEIPESKARVLRGHESEVFICAWNPSRDLLASGSGDSTARIWDMSDANTNSNQLVLRHCIQKGGAEVPSNKDVTSLDWNCDGSLLATGSYDGYARIWKTDGRLASTLGQHKGPIFALKWNKCGNYILSAGVDKTTIIWDASTGQCTQQFAFHSAPALDVDWQTNQAFASCSTDQRIHVCRLGVNEPIKTFKGHTNEVNAIKWCPQGQLLASCSDDMTLKIWSMNRDRCCHDLQAHSKEIYTIKWSPTGPGTNNPNTNLILASASFDSTVRLWDVERGSCIHTLTKHTEPVYSVAFSPDGKHLASGSFDKCVHIWSTQTGQLVHSYKGTGGIFEVCWNSKGTKVGASASDGSVFVLDLRKF q7kts4_drome Histone deacetylase DHDAC3 DmHDAC3 Dmel\CG2128 HDAC Hdac 3 dHDAC3 dmHDA402 hdac3 Dmel_CG2128 Hdac3 HDAC3 CG2128 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTDRRVSYFYNADVGNFHYGAGHPMKPQRLAVTHSLVMNYGLHKKMKIYRPYKASAQDMLRFHSDEYIAYLQQVTPQNIQCNSVAYTKYLAHFSVGEDCPVFDGLFDFCAMYTGASLEGAQKLNHNHSDICINWSGGLHHAKKFEASGFCYVNDIVIGILELLKYHPRVLYIDIDVHHGDGVQEAFYLTDRVMTASFHKYGNYFFPGTGDMYEIGAESGRYYSVNVPLKEGIDDQSYFQVFKPIISAIMDFYRPTAIVLQCGADSLAGDRLGCFSLSTKGHGECVKFVKELNVPTLVVGGGGYTLRNVARCWTHETSLLVDQDIENDLPATEYYDFFAPDFTLHPEINSRQDNANSKQYLELIVKHVYENLKMCQHSPSVQMVQTPPDVDLEELRSNREEASDPDVRISVADEDKLVDAKNEFYDGDQDQDKPDSAES q9vyk0_drome Smr CG12719 CG4013 Dmel\CG4013 E52 SMR SMRTER anon-WO0153538.73 l(1)G0060 l(1)G0361 lincRNA.983 smr Dmel_CG4013 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly 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 foxo1_mouse Forkhead box protein O1 Foxo1 Fkhr Foxo1a Forkhead box protein O1A Forkhead in rhabdomyosarcoma protein protein mouse Mus musculus Mouse MAEAPQVVETDPDFEPLPRQRSCTWPLPRPEFNQSNSTTSSPAPSGGAAANPDAAASLASASAVSTDFMSNLSLLEESEDFARAPGCVAVAAAAAASRGLCGDFQGPEAGCVHPAPPQPPPTGPLSQPPPVPPSAAAAAGPLAGQPRKTSSSRRNAWGNLSYADLITKAIESSAEKRLTLSQIYEWMVKSVPYFKDKGDSNSSAGWKNSIRHNLSLHSKFIRVQNEGTGKSSWWMLNPEGGKSGKSPRRRAASMDNNSKFAKSRGRAAKKKASLQSGQEGPGDSPGSQFSKWPASPGSHSNDDFDNWSTFRPRTSSNASTISGRLSPIMTEQDDLGDGDVHSLVYPPSAAKMASTLPSLSEISNPENMENLLDNLNLLSSPTSLTVSTQSSPGSMMQQTPCYSFAPPNTSLNSPSPNYSKYTYGQSSMSPLPQMPMQTLQDSKSSYGGLNQYNCAPGLLKELLTSDSPPHNDIMSPVDPGVAQPNSRVLGQNVMMGPNSVMPAYGSQASHNKMMNPSSHTHPGHAQQTASVNGRTLPHVVNTMPHTSAMNRLTPVKTPLQVPLSHPMQMSALGSYSSVSSCNGYGRMGVLHQEKLPSDLDGMFIERLDCDMESIIRNDLMDGDTLDFNFDNVLPNQSFPHSVKTTTHSWVSG hdac4_mouse Histone deacetylase 4 Hdac4 protein protein mouse Mus musculus Mouse MSSQSHPDGLSGRDQPVELLNPARVNHMPSTVDVATALPLQVAPTAVPMDLRLDHQFSLPLEPALREQQLQQELLALKQKQQIQRQILIAEFQRQHEQLSRQHEAQLHEHIKQQQEMLAMKHQQELLEHQRKLERHRQEQELEKQHREQKLQQLKNKEKGKESAVASTEVKMKLQEFVLNKKKALAHRNLNHCISSDPRYWYGKTQHSSLDQSSPPQSGVSASYNHPVLGMYDAKDDFPLRKTASEPNLKLRSRLKQKVAERRSSPLLRRKDGPVATALKKRPLDVTDSACSSAPGSGPSSPNSSSGNVSTENGIAPTVPSAPAETSLAHRLVTREGSVAPLPLYTSPSLPNITLGLPATGPAAGAAGQQDAERLALPALQQRILFPGTHLTPYLSTSPLERDGAAAHNPLLQHMVLLEQPPTQTPLVTGLGALPLHSQSLVGADRVSPSIHKLRQHRPLGRTQSAPLPQNAQALQHLVIQQQHQQFLEKHKQQFQQQQLHLSKIISKPSEPPRQPESHPEETEEELREHQALLDEPYLDRLPGQKEPSLAGVQVKQEPIESEEEEAEATRETEPGQRPATEQELLFRQQALLLEQQRIHQLRNYQASMEAAGIPVSFGSHRPLSRAQSSPASATFPMSVQEPPTKPRFTTGLVYDTLMLKHQCTCGNTNSHPEHAGRIQSIWSRLQETGLRGKCECIRGRKATLEELQTVHSEAHTLLYGTNPLNRQKLDSSLTSVFVRLPCGGVGVDSDTIWNEVHSSGAARLAVGCVVELVFKVATGELKNGFAVVRPPGHHAEESTPMGFCYFNSVAVAAKLLQQRLNVSKILIVDWDVHHGNGTQQAFYNDPNVLYMSLHRYDDGNFFPGSGAPDEVGTGPGVGFNVNMAFTGGLEPPMGDAEYLAAFRTVVMPIANEFAPDVVLVSSGFDAVEGHPTPLGGYNLSAKCFGYLTKQLMGLAGGRLVLALEGGHDLTAICDASEACVSALLGNELEPLPEKVLHQRPNANAVHSMEKVMDIHSKYWRCLQRLSSTVGHSLIEAQKCEKEEAETVTAMASLSVGVKPAEKRSEEEPMEEEPPL sik3 1164285 n/a 1164292 putative self putative self putative self putative self region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false drome Drosophila melanogaster (Fruit fly) Fruit fly phosphorylation phosphorylation reaction phosphorylation false true false Fig. 2B akt1-q4qqa7 1164285 n/a 1164293 enzyme enzyme unspecified role unspecified role drome Drosophila melanogaster (Fruit fly) Fruit fly n/a 1164292 enzyme target enzyme target substrate substrate unspecified role unspecified role Q4QQA7:p.[Tyr281Ala;Ser293Ala;Ser401Ala;Thr486Ala] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q4QQA7:p.Lys70Met mutation mutation certain certain sequence position certain certain certain sequence position certain false region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false drome Drosophila melanogaster (Fruit fly) Fruit fly phosphorylation phosphorylation reaction phosphorylation false false false Fig. 2D SIK3 Thr/Ser/Tyr mutations alter kinetics of interaction. Lsy-70 mutation makes SIK3 catalytically inactive. hdac4-q4qqa7 1164285 n/a 1164294 enzyme target enzyme target substrate substrate neutral component neutral component Q8I9J6:p.[Ser242Ala;Ser576Ala;Ser751Ala] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n/a 1164292 enzyme enzyme neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false drome Drosophila melanogaster (Fruit fly) Fruit fly phosphorylation phosphorylation reaction phosphorylation false false false Fig. 2B Ser mutations in HDAC4 alter kinetics of reaction. hdac4-foxo 1164286 n/a 1164294 unspecified role unspecified role bait bait n/a 1164295 unspecified role unspecified role prey prey physical association physical association false false false foxo-hdac4 1164287 n/a 1164294 enzyme enzyme neutral component neutral component n/a 1164295 enzyme target enzyme target substrate substrate neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region ac1res monoacetylated residue Acetyl Acetylation (N terminus, N epsilon of Lysine, O of Serine) (Ac) Acetylation Ac1Res undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false deacetylation deacetylation reaction deacetylation false false false Fig. 6E hdac4-foxo-1 1164288 n/a 1164294 unspecified role unspecified role bait bait n/a 1164295 unspecified role unspecified role prey prey physical association physical association false false false Supp. FIg. 6A Overexpression of constitutively active SIK3T.196E disrupted the FOXO:HDAC interaction ep300-foxo 1164289 n/a 1164295 enzyme target enzyme target substrate substrate neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false acetylation acetylation reaction acetylation false false false Supp. Fig. 6G hdac4-ebi 1164290 n/a 1164296 unspecified role unspecified role prey prey n/a 1164297 unspecified role unspecified role prey prey n/a 1164294 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1164298 unspecified role unspecified role prey prey association association false false false Supp. Fig. 6H foxo1-hdac4 1164291 n/a 1164299 unspecified role unspecified role bait bait n/a 1164300 unspecified role unspecified role prey prey physical association physical association false false false Supp. Fig. 7C