IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ chiolo-2011-2 Double-strand breaks in heterochromatin move outside of a dynamic HP1a domain to complete recombinational repair. drome-kc Drosophila melanogaster embryo KC cells kc Drosophila embryo KC cells fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Chiolo I., Minoda A., Colmenares SU., Polyzos A., Costes SV., Karpen GH. iechiolo@lbl.gov, karpen@fruitfly.org Cell (0092-8674) 2011 Only protein-protein interactions imex curation chiolo-2011-1 Double-strand breaks in heterochromatin move outside of a dynamic HP1a domain to complete recombinational repair. drome-kc Drosophila melanogaster embryo KC cells kc Drosophila embryo KC cells anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Chiolo I., Minoda A., Colmenares SU., Polyzos A., Costes SV., Karpen GH. iechiolo@lbl.gov, karpen@fruitfly.org Cell (0092-8674) 2011 Only protein-protein interactions imex curation chiolo-2011-3 Double-strand breaks in heterochromatin move outside of a dynamic HP1a domain to complete recombinational repair. drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Chiolo I., Minoda A., Colmenares SU., Polyzos A., Costes SV., Karpen GH. iechiolo@lbl.gov, karpen@fruitfly.org Cell (0092-8674) 2011 Only protein-protein interactions imex curation hp1_drome Heterochromatin protein 1 Su(var)205 HP1 CG8409 Non-histone chromosomal protein C1A9 antigen protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MGKKIDNPESSAKVSDAEEEEEEYAVEKIIDRRVRKGKVEYYLKWKGYPETENTWEPENNLDCQDLIQQYEASRKDEEKSAASKKDRPSSSAKAKETQGRASSSTSTASKRKSEEPTAPSGNKSKRTTDAEQDTIPVSGSTGFDRGLEAEKILGASDNNGRLTFLIQFKGVDQAEMVPSSVANEKIPRMVIHFYEERLSWYSDNED q7yu66_drome Structural maintenance of chromosomes protein 5 SMC5 Smc5 CG32438 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MAGDRALSSKQLAGRIHSVYCKDFVSYSEITFHPKHYLNVLTGPNGSGKSTIVSAIILGLGGEPILLDRSASVADYIQSNKTSATIIVRVYGRTPNTTETFRRIINSNGLSTFSVNDKDTSKKNFLAAVSSFNIQVSNLCQFLPQDRVQDFSKMNPQELLLNTMSSVCDEDLINSFNSLKQMRTEQANVHANREKEKSDLVKKQKRLEHLQMTVSQYKEREEVKQKLQVYSAKKLWVETQAGEAKAAEMKTQVKNAKTQSDKLKNQHDKLLPSQEQIEKEKESLRKALLEKTRLLENAVAEKAAIDGKMDSLKQGIYQKKYELEQNIKKSRRTATECDNLNQLVENKIYELETLNKSRPLIVSELERAKESCAAARGKAMEQYSRRRQLEQKLNDEMIPEITAYKLKIERLRNVKMQKIDEIRAKNPNLVVAMNWLAQNKQRYKLNVYDPMILELTVQNHEDAKFLENVVAQRDLFAFACEDKGDMSDLINELCVKQKLGVNVIYCAPSDRLVYSPKTPIDDLRSFGFRSYLVDLVTGPIPLINKLCASYSIHNIPIGTEAVGNYTSSIPKAIRVYFGGSKKFVVTASRYRSDTILTESSIRAKNQLITVDSQQLALVMKQCSEAVKESDSIKNAITQTDNEFERLQAVAKDEQEKRRKLDQKIAHFNSLKTEIETLQKKLEALRNSDSLDCLETNFCNSLHKDLKKIFDADAELCSCLKAIDRLIIEKNMAQTKVSIYMLQHETQIEALKESEEQSKAATRDFQQLLQCLENQISDVNKRKSAIQGLCHGEIPTSSKFPFKKEFRELENIDLPELREAIHDFQARLECMKSVNSEAISSYQGLQNEVKQLEEGIQESVNQAKSIESGMSNLYDKWEPKLNSLVETISTKFSEFMESIEYVGEVVLSKTDKYDFDSYGIQIMVQFRRGLQLQPLDKFIQSGGERAVSIAIYSLSLQHVTHVPFRCVDEINQGMDATNERHIFDLLLKEATKHGSAQYLFVTPKLLRDLNYNEHLCVSIVHNSKTVCHGMQFPMA q9vcb8_drome jnj CG5524 AAF56254 Dmel\CG5524 Huc-95DE SMC6 Smc6 huc95E huc[95D-E] huc[95DE] huc[95E] smc6 Dmel_CG5524 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MERSRRGRIRRMPSPGSESDGSTAEPTRKRSKQQFVAEAEPDFVEEETEKYANASTSQTARSRNKQARQTTLNMSQRSVNFNLTSELSIPNAFDRCGKVISMRLTNFMCHSNLFIEFGPNINFLVGNNGSGKSAVITALALGLTSSARATNRASSIQKLIKNGEVSATISITLSNSGLRPFKADIFGPHLTVVRQIRHSSSTYDLQDARGKSVSKKVSDIRRMLLCFGINVENPIFVLNQEAAREFLKELEPASNYKLLMKATQLDVCTSSLTECHALRRHFTQELEQLEKKKEMMIKHIAAEEEKLSILEDKEMVKENLQQCKTKLAWMAVTSYQNELNNLEHSIKLIENKKASLEQTTSKKESTQATMNQKLKEFEASKNQILATQKFQDERLKTAKKAVQDLLLEASQVKAKIGNAERRMREDQRSYDECEKLIGNYHADFNRVNEQREENANKIEMLKKQVVKSEEIIAQLRAEQQEIKRDITSVQERLDAVKNGRIQLHKSKQNISWEIEALSRNKSNKLSVYGEQTIQVVHALRTQYAGSNMHRMPRGPLGQYISAPNPKYRDLIENQLMHCLRSFIVGSDRERQSLRALLQNKFQGGNMPTIITSPFTDRVYDVSRNKVQPTTPNTTVLIDEISCDDPVVMNYLIDILRIETVLVTESKEIAEFLTSDTENVPPNLTRVLVPNLGLEYIPSPNYAVYSTRITPARYIQKNVDDRIRQLQMEQSDLQEKEPSLEIDYMQHKKVLENTQKVISQKSTMIGQHQSRNQKAMQKIMELQNFDYQELPEYDRLKSHLADSGEKIEKCRLEREMLQEKLLSIQHRQTELESTEAEERRALEGINKKLTALDTEAGEVESKMRSLDLHYEENTRRFQKTLQLERKMLGEKETVLSELEKARTEAEKLGEFIATTQTEEKIREAISRYKSKIKQVEELNYNPEELERGLAELRDELELQSRHLAVVDSVVKKLRMAYHQRAQLFQRSRHHYFTMVQFQFEQALAMRQFKVSFETSDKEKTWKINVFPPSGNETSNTRSLSGGERSFTTVSLLKGLWSTSDHPFYFLDEYDVFTDEVNRKFITEILIGEGLEWLSRQYCFLTPQDTKVEASNLITVHKLEAPER q9vvh7_drome E3 SUMO-protein ligase NSE2 Qjt qjt qjt-RA Dmel_CG13732 Dmel\CG13732 Nse2 Quijote CG13732 E3 SUMO-protein transferase NSE2 Non-structural maintenance of chromosomes element 2 homolog protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MEFNYLADSALSTLVENKKFFKEMSDFVSDFSDGGKIKKLLEQSVEQSVEHAVNVVHMKIKHQSLNKAMNQVKNSSATIEEFEEVWKERSKAVEQKRINVKNLHEFKNFVKAVESAAGQAGAEVNDQANGTAYDEDLIMEDSGSEVFSFYDPWSKALIKNPMRNKMCGHIYDRDSVMPIIMDRIGIRCPVLGCANLCYIQPDHLVQDANVQQKIQQSMSNAIVDVTASEEDEE h3_drome Histone H3 His3 His3:CG31613 His3:CG33857 His3:CG33860 His3:CG33863 His3:CG33866 CG31613 CG33803 CG33806 CG33809 CG33812 CG33815 CG33818 CG33821 CG33824 CG33827 CG33830 CG33833 CG33836 CG33839 CG33842 CG33845 CG33848 CG33851 CG33854 CG33857 CG33860 CG33863 CG33866 His3:CG33803 His3:CG33806 His3:CG33809 His3:CG33812 His3:CG33815 His3:CG33818 His3:CG33821 His3:CG33824 His3:CG33827 His3:CG33830 His3:CG33833 His3:CG33836 His3:CG33839 His3:CG33842 His3:CG33845 His3:CG33848 His3:CG33851 His3:CG33854 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA atrip_drome ATR-interacting protein mus304 mus304 CG7347 Mutagen-sensitive protein 304 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MAKRFSAMKDFARCKKPRLDVSVTRGSARPSPPRNNFDGILWDDDDDVILMATQLAEAEIEAEERKKKGGTEVDIGNSEVTFSEFAPTFQGSTSTQQMFPPPPPPQKKPTSLDMDAIFADDDDFDFLAVTLMDSEPQKMPEPKTSTSRITTSSISVQQKTTTTTTINATQSRQQEHQLKFLMDRIEALKRENAQLEKNLGDSKERNEIKSGEVSLLRDELKHLRQQLQASKMEKLALADETNRDCNKKVAEAAKQIAAKDIELKIKNAEFSKLKTQQKAHERSMNSSMSILQAAPDPLEKRLSLRLNRLNIHRSVPGLKTDNGSVFEYSENEDQTKKRRNHFELELKQLLLHYARLQAKPESVDNLLPRILSSVGKVFTEFASYAQSLDFPHNCMLYPYNPHNLEEEVHRISLTHQSCLYDNEKAVPLRRFIATLALICRREERISRGLTEWKENDLGLLDMAIEAITKLGFSYEVGQHFGLLEALTSLLNSLLQENALLQHNEELLFDLLKQLVFTRPSPWVFAELSSCFLSCLRHPQLMDKMCVNSPKDCFVSDRVRSVYRFGPDSCLLQVYAGLLELCFFSETPLRQDYFQLLLKIGGNHVRFAFECFKNPPDFILEMLPYFADDGDEDSSDGTLMKTGTSLSFNSTGAVQGSVSNGSTSASVSNPNQNSNSSTTQRGKGCECYVKLCLSAVTIVFQVMHQWMLHSRKAGTEEVGEISRIAVHLLSLVFHEYYLTCLFRDSEETTKHYLSLICNWWSEHANLLGFQSIHLRLLNQLVKAHFMLKPLHLEAKPNNPVNDLSEWKRIVKNADDQRAVKSAVTVDPSKLLNTDFFSALKREENTFE q7yu66-su_var_205 1156937 n/a 1156940 unspecified role unspecified role neutral component neutral component region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1156941 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Fig. 6A su_var_205-q9vcb8 1156937 n/a 1156942 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1156940 unspecified role unspecified role neutral component neutral component region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Fig. 6A q9vvh7-su_var_205 1156937 n/a 1156943 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1156940 unspecified role unspecified role neutral component neutral component region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Fig. 6A mus304-his3 1156938 n/a 1156940 unspecified role unspecified role prey prey n/a 1156944 unspecified role unspecified role prey prey lys-10 n6me3+lys N6,N6,N6-trimethyl-L-lysine (5S)-5-amino-5-carboxy-N,N,N-trimethylpentan-1-aminium 2-amino-6-(trimethylammonio)hexanoic acid 5-azanyl-5-carboxy-N,N,N-trimethylpentanazanium MOD_RES N6,N6,N6-trimethyllysine N(zeta)-trimethyllysine N6,N6,N6-trimethyl-L-lysine N6,N6,N6-trimethylated L-lysine N6,N6,N6-trimethyllysin-N6-ium N6,N6,N6-trimethyllysine cation epsilon-trimethyllysine N-trimethylation (of lysine) N6Me3+Lys Trimethyl tri-Methylation certain certain sequence position certain certain certain sequence position certain false n/a 1156945 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Supp Fig. 5C su_var_205-q7yu66 1156939 n/a 1156942 unspecified role unspecified role prey prey n/a 1156940 unspecified role unspecified role bait bait n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n-terminal strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1156941 unspecified role unspecified role prey prey association association false false false Fig. 6B