MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint yin-2011a-2 Structure of a TCR with high affinity for self-antigen reveals basis for escape from negative selection. in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Yin Y., Li Y., Kerzic MC., Martin R., Mariuzza RA. EMBO J. (0261-4189) 2011 Only protein-protein interactions imex curation yin-2011a-1 Structure of a TCR with high affinity for self-antigen reveals basis for escape from negative selection. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Yin Y., Li Y., Kerzic MC., Martin R., Mariuzza RA. EMBO J. (0261-4189) 2011 Only protein-protein interactions imex curation chain c Chain C protein protein human Homo sapiens Human mint chain d Chain D protein protein human Homo sapiens Human mint dra_human HLA class II histocompatibility antigen, DR alpha chain HLA-DRA MHC class II antigen DRA HLA-DRA1 protein protein human Homo sapiens Human MAISGVPVLGFFIIAVLMSAQESWAIKEEHVIIQAEFYLNPDQSGEFMFDFDGDEIFHVDMAKKETVWRLEEFGRFASFEAQGALANIAVDKANLEIMTKRSNYTPITNVPPEVTVLTNSPVELREPNVLICFIDKFTPPVVNVTWLRNGKPVTTGVSETVFLPREDHLFRKFHYLPFLPSTEDVYDCRVEHWGLDEPLLKHWEFDAPSPLPETTENVVCALGLTVGLVGIIIGTIFIIKGLRKSNAAERRGPL chain b Chain B protein protein human Homo sapiens Human mint The sequence has been withdrawn from uniprot. hla_dra-chain_c 1130794 chain c TCR alpha chain 1130796 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified chain d TCR beta chain 1130797 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified dra_human DR4 alpha chain 1130798 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1130794 homomint mint F1a hla_dra-chain_c-1 1130794 chain d TCR beta chain 1130797 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified mutation increasing mutation increasing interaction mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false chain c TCR alpha chain 1130796 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified dra_human DR4 alpha chain 1130798 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1130794 F1c homomint mint hla_dra-chain_c-2 1130794 chain c TCR alpha chain 1130796 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified chain d TCR beta chain 1130797 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified mutation increasing mutation increasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false dra_human DR4 alpha chain 1130798 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1130794 homomint mint F1e hla_dra-chain_c-3 1130794 chain c TCR alpha chain 1130796 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified chain d TCR beta chain 1130797 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified mutation increasing Thr55Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false dra_human DR4 alpha chain 1130798 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1130794 homomint mint F1f hla_dra-chain_c-4 1130794 chain c TCR alpha chain 1130796 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified chain d TCR beta chain 1130797 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified mutation increasing mutation increasing interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false dra_human DR4 alpha chain 1130798 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1130794 homomint mint F1g hla_dra-chain_c-5 1130794 chain c TCR alpha chain 1130796 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified chain d TCR beta chain 1130797 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified mutation increasing mutation increasing interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false dra_human DR4 alpha chain 1130798 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1130794 homomint mint F1h hla_dra-chain_c-6 1130794 chain d TCR beta chain 1130797 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified chain c TCR alpha chain 1130796 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified mutation decreasing mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false dra_human DR4 alpha chain 1130798 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1130794 F1d homomint mint chain_b-chain_c 1130795 chain d TCR beta chain 1130797 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified chain b DR4 beta chain 1130799 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified chain c TCR alpha chain 1130796 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified dra_human DR4 alpha chain 1130798 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false F2 T1 homomint mint