IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ wu-2011-1 Skin stem cells orchestrate directional migration by regulating microtubule-ACF7 connections through GSK3β. mouse-som_stem_cell Mouse somatic stem cell somatic_stem_cell Somatic (adult) stem cell anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wu X., Shen QT., Oristian DS., Lu CP., Zheng Q., Wang HW., Fuchs E. fuchslb@rockefeller.edu Cell (0092-8674) 2011 Only protein-protein interactions imex curation wu-2011-2 Skin stem cells orchestrate directional migration by regulating microtubule-ACF7 connections through GSK3β. in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Wu X., Shen QT., Oristian DS., Lu CP., Zheng Q., Wang HW., Fuchs E. Details of enzyme construct taken from manufacturer's website. fuchslb@rockefeller.edu Cell (0092-8674) 2011 Only protein-protein interactions imex curation gsk3b_mouse Glycogen synthase kinase-3 beta Gsk3b Serine/threonine-protein kinase GSK3B protein protein mouse Mus musculus Mouse MSGRPRTTSFAESCKPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAAASPPANATAASDTNAGDRGQTNNAASASASNST macf1_mouse Microtubule-actin cross-linking factor 1 Actin cross-linking family 7 Macf1 Acf7 Aclp7 Macf protein protein mouse Mus musculus Mouse MSSSDEETLSERSCRSERSCRSERSYRSERSGSLSPCPPGDTLPWNLPLHEQKKRKSQDSVLDPAERAVVRVADERDRVQKKTFTKWVNKHLMKVRKHINDLYEDLRDGHNLISLLEVLSGIKLPREKGRMRFHRLQNVQIALDFLKQRQVKLVNIRNDDITDGNPKLTLGLIWTIILHFQISDIYISGESGDMSAKEKLLLWTQKVTAGYTGVKCTNFSSCWSDGKMFNALIHRYRPDLVDMERVQVQSNRENLEQAFEVAERLGVTRLLDAEDVDVPSPDEKSVITYVSSIYDAFPKVPEGGEGISATEVDSRWQEYQSRVDSLIPWIRQHTILMSDKSFPQNPVELKALYNQYIHFKETEILAKEREKGRIKELYKLLEVWIEFGRIKLPQGYHPNHVEEEWGKLIVEMLEREKSLRPAVERLELLLQIANKIQNGALNCEEKLTLAKNTLQADAAHLESGQPVQCESDVIMYIQECEGLIRQLQVDLQILRDEKYYQLEELAFRVMRLQDELVTLRLECTNLYRKGHFSSLELVPPSTLTTTHLKAEPLNKTTHSSSTSWFRKPMTRTELVSISSSEDEGNLRFVYELLSWVEEMQMKLERAEWGNDLPSVELQLETQQHIHTSVEELGSSVKEARLYEGKMSQNFHTSYVETLGKLETQYCKLKETSSFRMRHLQSLHKFVSRATAELIWLNGKEEEELACDWSDSNPNISAKKTYFSELTMELEGKQDVFRSLQDTAEVLSLENHPAKQTVEAYSAAVQSQLQWMKQLCLCVEQHVKENAAYFQFFSDARDLESFLRNLQDSIKRKYTCDRSTSLSRLEDLLQDSMDEKEQLIQSKSSVASLVGRSKTIVQLKPRNPDHVLKSTLSVKAICDYRQIEITICKNDECVLEDNSQRTKWKVISPTGNEAMVPSVCFLIPPPNKEAIEMASRVEQSYQKVMALWHQLHINTKSLISWNYLRKDLDTVQTWSLEKLRSLAPGECHQVMKNLQAHYEDFLQDSHDSALFSVADRLRIEEEVEACKAHFQHLMKSLENEDKEETLAKVYISELKNIRLLLEECEQRLLKQIQSPASSKTDRDARQDITLRIAEQEHTQEDLQHLRSDLDAISMKCNVFLQQSPSGSSATTLRSELNLMVEKMDHVYGLSTVYLNKLKTIDVIVRSMQDAELLVKGYEIKLSQEEAVPADLSALESHRTTLQHWLSDVKDKNSVFSVLDEEITKAKKVAEQLRHPASEPNLDLERYQEKGSQLQERWHRVIAQLETRQSEVESIQEVLRDYRACHGTLIKWIEETTAQQEMMKPGQAEDSRVLSEQLSQQTELFAEIERNQTKLDQCQKFSQQYSTIVKDYELQLMTYKAFVESQQKSPGKRRRMISSSDAITQEFMDLRTRYTALVTLTTQHVKYISDALRRLEEEEKVVEEEKQEHVEKVKDLLGWVSTLARNTQGTTTSSHTSASADIEKAILEQQVLAEELTTKKEQVSEAIKTSQIFLAKHGHKLSEGEKEQISEQLRVLNKTYHDLCDGSANQLQQLQSELAQQTEQKGCRAVAGVIDLGTVEIFPIFRAMQKGLIDQDTGLVLLESQIIMSGLIDPENSEKLSLEEGLTRNFINLPIYQQLLGLRDSLSLVSRLTGTLGSLSVVEAIEKKIISERLGLKVLEVHLATGGFSLPPSENCINLEEAFHQGFIASSLHSELQSHLRSSKNLIDPNTAEKVGLLDLMQRCIIHQESGLKLLPVKQLAGGMVSLKSGRKVSIFRAVQEGLIDRQVTVRLLEAQLFAGGIVDPRTGHRLTVEEAVRHNLIDQDMACAILIRQLQTGGIIDTVTGDRMTIDEAVTNNLVAAKIALVILESLWSFMGLLLPESGEILPITDALEQGIVSTELAHKILHNRQQIEALFLPTLTEIWSWEKATESGILDKDLVNNLRSVCIPDMMPHIQLADSAEQSKVGFAAGKPPVSGPREEGSSHGEKLLFQLMTHSYIHAHTGQRLLLLDQELVEMLTSRDDCQVILPEVFEIQHQRLNTSEALQELYTGTISQISSAKHPRKPCESQFLSQNKDYPSQENCTEAKGERSVVGIECSPAESPERELFLKEQEAIIENVGSLKVINKVKLKLQRPLLGSRKEEQAETLREENISGDPLLVECPEESEGKDLSTEKSKCQTPTKCSFTCHKEQVKTIKDIPSETGTSLIKSQNQMSQFQVDTSVGLRSEFKSEHDMNVNSLEKELKEELLVKDGHKQSQEGQSVADGQTVALEKTDTEDNADEPALLHSSPFEDATLSTLSAQLQDGGIFNEETGQKLLLNEAIAQGLVSSHTAVKLMGKLNMFRGFFDSQTCESLTTEEVIDEGLMDEKLLYNVLMSDKAISGILDPRTHSLCSVKEAVAAGLLDKETATRILEGQVITGGIVDLKRGKKLSVTLASNLGLVDTADQTELINLEKATKGRGAEKAVKERLIELQMETAGLMDPESKAPLTVLQSIDRGILEREAAVYLLTKQVLDGGIIHHISGLRLSVDNAFKHGLIGEDMARQLRKVENFIHYQFFHPQTKEALSFSEAIKLDLVSPDLKREIQEIQDFSGNLGDFIYGQKLTLAKTNKEESLANKTELPSGVMHGVIDPENCTIIPYSELVKKCRIDTESGWRYLEVIPFSDIKDEAGNNVLTPPEAIQLGKVDFASALKVLEAQANTGGIIDMATGKRVTLASALEKKLLDENMARIIASHQMLSGGIIDIYSDQRVTLNDAVEKRLISPELAAMIQVDPLAEQGGTGVCELKGDFLRKELLSESSKTPRESYSKEKHEAVLQAGSLCAPEKAGIRGSNGEKAEKGRKISVEMEGQRQDEKASSDSKVSASILSPFGFEGESSYQVSVTHPCSESCDLKPREETRSCMKKCAVVERDKVVTQIKMVSHVKQSTSGLDAEEARERQGRMVSKEQGSHYETAGNLLSERSVRVDRRVRREMGGEQSVQMSREAAVLSEEESDQEVTIGDEPDSFVKSQSMKMIGNDKGKEAGIEKDISVVCKIEGFPSQMTSKDASLTNQDALPFYTEGETKTVNLCSILKPGEKLSQETASTVQKEPLSSEIPRPERLNSQESDEEPQISDVPHISKGDMAAQITTRQETTDVQDLYITSKSSETKDKIFPSKNYIEKLHQEIPMDPTRSHKLKEATISTLETEGISYLDSSDIKSLCEDSKADHKSCGHQKSKVTTTQAKKSLEVVDLLVRDTEEGSSEDRVGQRGPRVLASLLPEKLPTRTVQSENIRQHDAVIPAISEIREEMALSLPCSVVKVDGKIPKEKHKEILGDEQGPFMAIPSGKGIEGVNPEPCRATQNVFTRRLCLEHDEKLVSYLSLLRDIEMRTKQIQPLELNVAELQDLLGQAKELDRELKDLSTVVSQELECVDRIVISQPQEVPAQLLKALEKDAKNLQKSLDSVSDSWSSRFLHLQSAVEVKKATVLNRHKELQGKLQDLRAWVGRASLTLNSKGCDTETDADSLSHTLQPYKDMKQSMAERKSQLDALALDIQLFISEHPQDLSLQQNQEMLQFLSELQRSFQGLVEHTAAQKDVVQGHLQQVQQEVQVKTLQKQQDTCHKKLEDLCNWVGQAERALERHQGGSSSGKSSLLCAEPKVDLKDLQGDIQSHSTSFATAVKDIEGFLEENQTKLSPQELTALREKLHQAKEQYEVLQERTRVAQKELEEAVTSALQQETEKSKAATELAENKRKIDALLDWVGLLWGHLRESHRLAFQEWSSSLELAMEKQTLAATDGHVDVNQVPETLDRQYELMKARHQELLSQQQNFIVATQSVQSFLDQHSHNLTPEERQKLQEKLGELKEQYAASLARSEAELKQTQALRDELQKFLQDHKEFENWLQQSENELDSMHKGGSSPEALNSLLKRQGSFSEDVISHKGDLRFVTISGQKVLETENNFEEGQEPSATRNLVNEKLKDATERYTTLHSKCIRLGSHLSMLLGQYQQFQSSADSLQAWVLTCEASVGKLLSDTVASDPGVLQQQLATTKQLQEELAEHQVPVEKLQKAAHDLLDIEGEPALDCRPIQETTDSISSRFQNLSCSLDERSALLQKAIAQSQSVQESMESLLQSIREVEQNLERDQVASLSSGVIQEALANNMKLKQDIARQKSSLEATHDMVTRFMETADSNSASVLQGKLAELSQRFQQLQLQQQEKESNLKKLLPQAEMFEQLSNKLQQFMENKSRLLASGNQPDQDIAHFSQQIQELTLAMEDQKENLDTLEHLVTTLGSCGFALDLSQHQDKIQNLKKDFTELQKTVQEREKDASTCQEQLDEFRKLIRTFQKWLKETEGNVPPAKTFVSAKELEKQIEHLKDLISDWESKGALLGEINAKGTALESLIMDITAPDSQAKTGSILPPVGSSVGSVNGYHTCKDLTEIQCDMFDVNSKYEKLWEVLRERQESLQTVFSRMEEVQKEASSVLQWLESKEEVLKAMDATLSPTKTETVKAQAESNKAFLAELEQNSPKIQKVKEALAGLLKTYPNSQEAENWKKMQEDLNSRWEKATEVTVARQKQLEESASHLACFQAAESQLRPWLMEKELMMGVLGPLSIDPNMLKQQVQFMLKEFEARRQQHEQLNEAAQGILTGPGDMSPSASQVHKDLQSISQKWVELTDKLNSRSSQIDQAIVKSTQYQDLLQDLSEKVKAIGQRLSGQSAISTQPEAVKQQLEETSEIRSDLGQLDNEIKEAQTLCQELSLLIGEQYLKDELKKRLETVALPLQGLEDLAADRMNRLQAALASTQQFQQMFDELRTWLDEKQSQQAKNCPISAKLERLQCQLQENEEFQKNLNQHSGSYEVIVAEGEALLLSVPPGEEKKTLQNQLVELRSHWEDLSKKTANRQSRLKDCMQKAQKYQGHVEDLVPWIDECKSKMSELQVTLDPVQLESSLLRSKAMLNEAEKRRSLLEILNSAADILINSSEIDEDEIRDEKAGLNQNMDAITEELQAKTSSLEEMTQRLKEFQESFKNIEKKVEGAKHQLEIFDALGSQACSNKNLEKLKAQQEVLQALEPQVDYLRNFTQGLVEDAPDGSDASPLVHQAEVAQQEFLEVKQRVSSSCLTMENKLEGIGQFHCRVREMFSQLADLDDELDGMGAIGRDTDSLQSQIEDVRLFLNKIQALRFDIEDSEAECRKMLEEEGTLDLLGLKRELEALNKQCGKLTERGKVRQEQLELTLGRVEDFYRKLKALNDAATAAEEGEALQWIVGTEVDVINQQLADFKLFQKDQVDPLQVKLQQVNGLGQGLIQSAGKNCDVQGLEHDMDEINTRWNTLNKKVAQRIAQLQEALLHCGKFQDALEPLLSWLTDTEELIANQKPPSAEYKVVKAQIQEQKLLQRLLDDRKATVDMLQAEGGRIAQSAELADREKITGQLESLERRWTDLLSKAAARQKQLEDILVLAKQFHETAEPISDFLSVTANKLANSEPVGTQTAKIHQQIIRHKALNEEIINRKKNVDQAIKNGQALLKQTTGEEVLLIQEKLDGIKTRYADITLTSSKALRTLEQARQLATKFHSTYEELTGWLREAEEELAASGGQSPTGEQIPQFQQRQKELKKEVMEHRLVLDTVNEVSHALLELVPWRAREGLDKLVSDANEQYKLISDTVGQRVDEIDAAIQRSQQYEQAADAELAWVAETKRKLMALGPIRLEQDQTTAQLQVQKAFSIDIIRHKDSMDELFSHRGEIFSTCGEEQKAVLQEKTECLIQQYEAVSLLNSERYARLERAQVLVNQFWETYEELSPWAEETLALIAQLPPPAVDHEQLRQQQEEMRQLRESIAEHKPHIDKILKIGPQLKELNPEEGKMVEEKYQKAENMYAQIKDEVRQRALALDEAVSQSAQFHDKIEPMLETLENLSSRLRMPPLIPAEVDKIRECISDNKSATVELEKLQPSFEALKRRGEELIGRSQGADKDLAAKEIQDKLDQMVFFWEDIKARSEEREIKFLDVLELAEKFWYDMAALLTTIKDTQDIVHDLESPGIDPSIIKQQVEAAETIKEETDGLHEELEFIRILGADLIFACGETEKPEVKKSIDEMNNAWENLNKTWKERLEKLEDAMQAAVQYQDTLQAMFDWLDNTVIKLCTMPPVGTDLNTVKDQLNEMKEFKVEVYQQQIEMEKLNHQGELMLKKATDETDRDIIREPLTELKHLWENLGEKIAHRQHKLEGALLALGQFQHALEELMSWLTHTEELLDAQRPISGDPKVIEVELAKHHVLKNDVLAHQATVATVNKAGSELLESSAGDDASSLRSRLETMNQCWESVLQKTEEREQQLQSTLQQAQGFHSEIEDFLLELNRMENQLSASKPTGGLPETAREQLDTHMELHSQLRAKEEIYNQLLDKGRLMLLSRGDSGSGSKTEQSVALLEQKWHAVSSKVEERKSKLEEALSLATEFQNSLQEFINWLTLAEQSLNIASPPSLILNTVLSQIEEHKVFANEVNDHRDQIIELDQTGNQLKFLSQKQDVVLIKNLLVSVQSRWEKVVQRSIERGRSLDDARKRAKQFHEAWKKLIDWLEDAESHLDSELEISNDPDKIKLQLSKHKEFQKTLGGKQPVYDTTIRTGRALKEKTLLAGDTQKLDNLLGEVRDKWDTVCGKSVERQHKLEEALLFSGQFMDALQALVDWLYKVEPQLAEDQPVHGDLDLVMNLMDAHKVFQKELGKRTGTVQVLKRSGRELIEGSRDDTTWVKGQLQELSTRWDTVCKLSVSKQSRLEQALKQAEEFRDTVHMLLEWLSEAEQTLRFRGALPDDTEALQSLIDTHKEFMKKVEEKRVDVNTAVAMGEAILAVCHPDCITTIKHWITIIRARFEEVLTWAKQHQQRLETALSELVANAELLEELLAWIQWAETTLIQRDQEPIPQNIDRVKALITEHQSFMEEMTRKQPDVDRVTKTYKRKSVEPTHAPFMEKSRSGSRKSLNQPTPPPMPILSQSEAKNPRINQLSARWQQVWLLALERQRKLNDALDRLEELCPELKEFANFDFDVWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILASKFPTTKLEMTAVADIFDRDGDGYIDYYEFVAALHPNKDAYRPTTDADKIEDEVTRQVAQCKCAKRFQVEQIGENKYRFFLGNQFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCRARGRTNIELREKFILPEGASQGMTPFRSRGRRSKPSSRAASPTRSSSSASQSNHSCTSMPSSPATPASGTKVISSSGSKLKRPTPAFHSSRTSLAGDTSNSSSPASTGAKANRADPKKSASRPGSRAGSRAGSRASSRRGSDASDFDLLETQSACSDTSESSAAGGQGSSRRGLTKPSKIPTMSKKTTTASPRTPGPKR g1t8e2_rabit Glycogen synthase kinase-3 beta GSK3B protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MSGRPRTTSFAESCKPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKDSSGTGRFTSGVRVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAAASTPTNATAASDGLFNEQFFALNSKGQISSLI Similar to P49841 macf1-gsk3b 1129621 n/a 1129623 unspecified role unspecified role prey prey n/a 1129624 unspecified role unspecified role bait bait physical association physical association false false false Fig. 2A gsk3b-macf1-1 1129622 n/a 1129624 enzyme target enzyme target substrate substrate neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false n/a 1129625 enzyme enzyme neutral component neutral component ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false Fig. 2B Phosphorylation of Macf1 Exact coordinates of MACF1 C-terminal domain not given and has been inferred from the information in the text.