UniProt UniProt http://www.uniprot.org liu-2011b-1 Molecular Basis of Wnt Activation via the DIX Domain Protein Ccd1. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Liu YT., Dan QJ., Wang J., Feng Y., Chen L., Liang J., Li Q., Lin SC., Wang ZX., Wu JW. jiaweiwu@mail.tsinghua.edu.cn J. Biol. Chem. (0021-9258) 2011 mimix curation liu-2011b-5 Molecular Basis of Wnt Activation via the DIX Domain Protein Ccd1. in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Liu YT., Dan QJ., Wang J., Feng Y., Chen L., Liang J., Li Q., Lin SC., Wang ZX., Wu JW. jiaweiwu@mail.tsinghua.edu.cn J. Biol. Chem. (0021-9258) 2011 mimix curation liu-2011b-2 Molecular Basis of Wnt Activation via the DIX Domain Protein Ccd1. in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Liu YT., Dan QJ., Wang J., Feng Y., Chen L., Liang J., Li Q., Lin SC., Wang ZX., Wu JW. jiaweiwu@mail.tsinghua.edu.cn J. Biol. Chem. (0021-9258) 2011 mimix curation liu-2011b-3 Molecular Basis of Wnt Activation via the DIX Domain Protein Ccd1. in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Liu YT., Dan QJ., Wang J., Feng Y., Chen L., Liang J., Li Q., Lin SC., Wang ZX., Wu JW. jiaweiwu@mail.tsinghua.edu.cn J. Biol. Chem. (0021-9258) 2011 mimix curation liu-2011b-4 Molecular Basis of Wnt Activation via the DIX Domain Protein Ccd1. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Liu YT., Dan QJ., Wang J., Feng Y., Chen L., Liang J., Li Q., Lin SC., Wang ZX., Wu JW. jiaweiwu@mail.tsinghua.edu.cn J. Biol. Chem. (0021-9258) 2011 mimix curation dvl2_mouse Segment polarity protein dishevelled homolog DVL-2 Dvl2 DSH homolog 2 protein protein mouse Mus musculus Mouse MAGSSAGGGGVGETKVIYHLDEEETPYLVKIPVPAERITLGDFKSVLQRPAGAKYFFKSMDQDFGVVKEEISDDNARLPCFNGRVVSWLVSSDTPQPEVAPPAHESRTELVPPPPPLPPLPPERTSGIGDSRPPSFHPNVSSSHENLEPETETESVVSLRRDRPRRRDSSEHGAGGHRPGGPSRLERHLAGYESSSTLMTSELESTSLGDSDEDDTMSRFSSSTEQSSASRLLKRHRRRRKQRPPRMERTSSFSSVTDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDMNFENMSNDDAVRVLRDIVHKPGPIVLTVAKCWDPSPQAYFTLPRNEPIQPIDPAAWVSHSAALTGAFPAYPGSSSMSTITSGSSLPDGCEGRGLSVHMDMASVTKAMAAPESGLEVRDRMWLKITIPNAFLGSDVVDWLYHHVEGFPERREARKYASGLLKAGLIRHTVNKITFSEQCYYVFGDLSGGCESYLVNLSLNDNDGSSGASDQDTLAPLPGATPWPLLPTFSYQYPAPHPYSPQPPPYHELSSYTYGGGSASSQHSEGSRSSGSTRSDGGAGRTGRPEERAPESKSGSGSESELSSRGGSLRRGGEPGGTGDGGPPPSRGSTGAPPNLRALPGLHPYGAPSGMALPYNPMMVVMMPPPPPPVSTAVQPPGAPPVRDLGSVPPELTASRQSFHMAMGNPSEFFVDVM dixc1_human Dixin DIXDC1 CCD1 KIAA1735 DIX domain-containing protein 1 Coiled-coil protein DIX1 protein protein human Homo sapiens Human MLACLTRGNLLDVLQEGFNEQQLQAYVAWVNAQLKKRPAVKPVQDLRQDLRDGVILAYLIEIVAGEKLSGVQLSPGNQQEMKNNVEKVLQFVASKKIRMHQTSAKDIVDGNLKSIMRLVLALAAHFKPGSSRTVNQGRDSRAPLQSHRPHCATAVAQGAAAALADVCHDMSRSGRDVFRYRQRNSSMDEEIENPYWSVRALVQQYEGQQRSPSESSCSSLTSPSPIHSAKSESIITQSEEKADFVIIPAEGIENRTEGTDSPLSRDWRPGSPGTYLETSWEEQLLEQQEYLEKEMEEAKKMISGLQALLLNGSLPEDEQERPLALCEPGVNPEEQLIIIQSRLDQSMEENQDLKKELLKCKQEARNLQGIKDALQQRLTQQDTSVLQLKQELLRANMDKDELHNQNVDLQRKLDERNRLLGEYKKELGQKDRLLQQHQAKLEEALRKLSDVSYHQVDLERELEHKDVLLAHCMKREADEATNYNSHNSQSNGFLLPTAGKGATSVSNRGTSDLQLVRDALRSLRNSFSGHDPQHHTIDSLEQGISSLMERLHVMETQKKQERKVRVKSPRTQVGSEYRESWPPNSKLPHSQSSPTVSSTCTKVLYFTDRSLTPFMVNIPKRLEEVTLKDFKAAIDREGNHRYHFKALDPEFGTVKEEIFHDDDAIPGWEGKIVAWVEEDHGEN dvl1_mouse Segment polarity protein dishevelled homolog DVL-1 Dvl1 Dvl DSH homolog 1 protein protein mouse Mus musculus Mouse MAETKIIYHMDEEETPYLVKLPVAPERVTLADFKNVLSNRPVHAYKFFFKSMDQDFGVVKEEIFDDNAKLPCFNGRVVSWLVLAEGAHSDAGSQGTDSHTDLPPPLERTGGIGDSRPPSFHPNVASSRDGMDNETGTESMVSHRRERARRRNRDEAARTNGHPRGDRRRDLGLPPDSASTVLSSELESSSFIDSDEEDNTSRLSSSTEQSTSSRLVRKHKCRRRKQRLRQTDRASSFSSITDSTMSLNIITVTLNMERHHFLGISIVGQSNDRGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDVNFENMSNDDAVRVLREIVSQTGPISLTVAKCWDPTPRSYFTIPRADPVRPIDPAAWLSHTAALTGALPRYGTSPCSSAITRTSSSSLTSSVPGAPQLEEAPLTVKSDMSAIVRVMQLPDSGLEIRDRMWLKITIANAVIGADVVDWLYTHVEGFKERREARKYASSMLKHGFLRHTVNKITFSEQCYYVFGDLCSNLASLNLNSGSSGASDQDTLAPLPHPSVPWPLGQGYPYQYPGPPPCFPPAYQDPGFSCGSGSAGSQQSEGSKSSGSTRSSHRTPGREERRATGAGGSGSESDHTVPSGSGSTGWWERPVSQLSRGSSPRSQASAVAPGLPPLHPLTKAYAVVGGPPGGPPVRELAAVPPELTGSRQSFQKAMGNPCEFFVDIM dvl2-dvl2-2 1135795 n/a 1135800 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1135800 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false fig 2B, fig 3, fig 4, fig 7A dixdc1-dvl2 1135795 n/a 1135801 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1135800 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false fig 7a dvl2-dvl2-3 1135796 n/a 1135800 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1135800 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false supplemental fig 2 forms oligomers dixdc1-dvl2-2 1135796 n/a 1135801 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1135800 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false fig 7b dvl2-dixdc1 1135797 n/a 1135801 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1135800 unspecified role unspecified role bait bait n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false fig 6 dixdc1-dvl2-1 1135798 n/a 1135800 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1135801 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false supplementary fig S4 dvl1-dvl1 1135799 n/a 1135802 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1135802 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 2.87 A R value 0.232 R free 0.265