IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ dhennin_duthille-2011-2 The tumor suppressor hTid1 inhibits STAT5b activity via functional interaction. mouse-ba_f3 Mouse pro B cells IL-3 dependent mouse-baf3 EBI-6922762 ba_f3 Mouse pro B cells IL-3 dependent BA/F3 baf3 Mouse pro B cell line. IL-3 dependent murine pro B cell line established from peripheral blood; apparently derived from BALB/c mouse. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Dhennin-Duthille I., Nyga R., Yahiaoui S., Gouilleux-Gruart V., Regnier A., Lassoued K., Gouilleux F. Apoptosis - Interactions involving proteins with a function related to apoptosis fabrice.gouilleux@univ-tours.fr Human Jurkat T cell cDNA J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation dhennin_duthille-2011-3 The tumor suppressor hTid1 inhibits STAT5b activity via functional interaction. human-697 Homo sapiens 697 Dexamethasone-resistant human Pre-B leukemia 697 cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Dhennin-Duthille I., Nyga R., Yahiaoui S., Gouilleux-Gruart V., Regnier A., Lassoued K., Gouilleux F. Apoptosis - Interactions involving proteins with a function related to apoptosis fabrice.gouilleux@univ-tours.fr Human Jurkat T cell cDNA J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation dhennin_duthille-201 The tumor suppressor hTid1 inhibits STAT5b activity via functional interaction. chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Dhennin-Duthille I., Nyga R., Yahiaoui S., Gouilleux-Gruart V., Regnier A., Lassoued K., Gouilleux F. Apoptosis - Interactions involving proteins with a function related to apoptosis fabrice.gouilleux@univ-tours.fr Human Jurkat T cell cDNA J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation dhennin_duthille-201 The tumor suppressor hTid1 inhibits STAT5b activity via functional interaction. ecolx Escherichia coli 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Dhennin-Duthille I., Nyga R., Yahiaoui S., Gouilleux-Gruart V., Regnier A., Lassoued K., Gouilleux F. Apoptosis - Interactions involving proteins with a function related to apoptosis fabrice.gouilleux@univ-tours.fr Human Jurkat T cell cDNA J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation dhennin_duthille-201 The tumor suppressor hTid1 inhibits STAT5b activity via functional interaction. ecolx Escherichia coli 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Dhennin-Duthille I., Nyga R., Yahiaoui S., Gouilleux-Gruart V., Regnier A., Lassoued K., Gouilleux F. Apoptosis - Interactions involving proteins with a function related to apoptosis fabrice.gouilleux@univ-tours.fr Human Jurkat T cell cDNA J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation dhennin_duthille-2011-1 The tumor suppressor hTid1 inhibits STAT5b activity via functional interaction. human-jurkat Homo sapiens jurkat Human T cell leukemia anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Dhennin-Duthille I., Nyga R., Yahiaoui S., Gouilleux-Gruart V., Regnier A., Lassoued K., Gouilleux F. Apoptosis - Interactions involving proteins with a function related to apoptosis fabrice.gouilleux@univ-tours.fr Human Jurkat T cell cDNA J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation sta5b_mouse Signal transducer and activator of transcription 5B Stat5b protein protein mouse Mus musculus Mouse MAMWIQAQQLQGDALHQMQALYGQHFPIEVRHYLSQWIESQAWDSIDLDNPQENIKATQLLEGLVQELQKKAEHQVGEDGFLLKIKLGHYATQLQSTYDRCPMELVRCIRHILYNEQRLVREANNGSSPAGSLADAMSQKHLQINQTFEELRLITQDTENELKKLQQTQEYFIIQYQESLRIQAQFAQLGQLNPQERMSRETALQQKQVSLETWLQREAQTLQQYRVELAEKHQKTLQLLRKQQTIILDDELIQWKRRQQLAGNGGPPEGSLDVLQSWCEKLAEIIWQNRQQIRRAEHLCQQLPIPGPVEEMLAEVNATITDIISALVTSTFIIEKQPPQVLKTQTKFAATVRLLVGGKLNVHMNPPQVKATIISEQQAKSLLKNENTRNDYSGEILNNCCVMEYHQATGTLSAHFRNMSLKRIKRSDRRGAESVTEEKFTILFDSQFSVGGNELVFQVKTLSLPVVVIVHGSQDNNATATVLWDNAFAEPGRVPFAVPDKVLWPQLCEALNMKFKAEVQSNRGLTKENLVFLAQKLFNISSNHLEDYNSMSVSWSQFNRENLPGRNYTFWQWFDGVMEVLKKHLKPHWNDGAILGFVNKQQAHDLLINKPDGTFLLRFSDSEIGGITIAWKFDSQERMFWNLMPFTTRDFSIRSLADRLGDLNYLIYVFPDRPKDEVYSKYYTPVPCEPATAKAADGYVKPQIKQVVPEFANASTDAGSGATYMDQAPSPVVCPQAHYNMYPPNPDSVLDTDGDFDLEDTMDVARRVEELLGRPMDSQWIPHAQS q96ey1-1 DnaJ homolog subfamily A member 3, mitochondrial Tid-1(L) DNAJA3 HCA57 TID1 DnaJ protein Tid-1 Hepatocellular carcinoma-associated antigen 57 Tumorous imaginal discs protein Tid56 homolog protein protein human Homo sapiens Human MAARCSTRWLLVVVGTPRLPAISGRGARPPREGVVGAWLSRKLSVPAFASSLTSCGPRALLTLRPGVSLTGTKHNPFICTASFHTSAPLAKEDYYQILGVPRNASQKEIKKAYYQLAKKYHPDTNKDDPKAKEKFSQLAEAYEVLSDEVKRKQYDAYGSAGFDPGASGSQHSYWKGGPTVDPEELFRKIFGEFSSSSFGDFQTVFDQPQEYFMELTFNQAAKGVNKEFTVNIMDTCERCNGKGNEPGTKVQHCHYCGGSGMETINTGPFVMRSTCRRCGGRGSIIISPCVVCRGAGQAKQKKRVMIPVPAGVEDGQTVRMPVGKREIFITFRVQKSPVFRRDGADIHSDLFISIAQALLGGTARAQGLYETINVTIPPGTQTDQKIRMGGKGIPRINSYGYGDHYIHIKIRVPKRLTSRQQSLILSYAEDETDVEGTVNGVTLTSSGGSTMDSSAGSKARREAGEDEEGFLSKLKKMFTS q96ey1-2 DnaJ homolog subfamily A member 3, mitochondrial DNAJA3 HCA57 TID1 DnaJ protein Tid-1 Hepatocellular carcinoma-associated antigen 57 Tumorous imaginal discs protein Tid56 homolog Tid-1(S) protein protein human Homo sapiens Human MAARCSTRWLLVVVGTPRLPAISGRGARPPREGVVGAWLSRKLSVPAFASSLTSCGPRALLTLRPGVSLTGTKHNPFICTASFHTSAPLAKEDYYQILGVPRNASQKEIKKAYYQLAKKYHPDTNKDDPKAKEKFSQLAEAYEVLSDEVKRKQYDAYGSAGFDPGASGSQHSYWKGGPTVDPEELFRKIFGEFSSSSFGDFQTVFDQPQEYFMELTFNQAAKGVNKEFTVNIMDTCERCNGKGNEPGTKVQHCHYCGGSGMETINTGPFVMRSTCRRCGGRGSIIISPCVVCRGAGQAKQKKRVMIPVPAGVEDGQTVRMPVGKREIFITFRVQKSPVFRRDGADIHSDLFISIAQALLGGTARAQGLYETINVTIPPGTQTDQKIRMGGKGIPRINSYGYGDHYIHIKIRVPKRLTSRQQSLILSYAEDETDVEGTVNGVTLTSSGKRSTGN dnja3_human DnaJ homolog subfamily A member 3, mitochondrial DNAJA3 TID1 HCA57 Tumorous imaginal discs protein Tid56 homolog DnaJ protein Tid-1 Hepatocellular carcinoma-associated antigen 57 protein protein human Homo sapiens Human MAARCSTRWLLVVVGTPRLPAISGRGARPPREGVVGAWLSRKLSVPAFASSLTSCGPRALLTLRPGVSLTGTKHNPFICTASFHTSAPLAKEDYYQILGVPRNASQKEIKKAYYQLAKKYHPDTNKDDPKAKEKFSQLAEAYEVLSDEVKRKQYDAYGSAGFDPGASGSQHSYWKGGPTVDPEELFRKIFGEFSSSSFGDFQTVFDQPQEYFMELTFNQAAKGVNKEFTVNIMDTCERCNGKGNEPGTKVQHCHYCGGSGMETINTGPFVMRSTCRRCGGRGSIIISPCVVCRGAGQAKQKKRVMIPVPAGVEDGQTVRMPVGKREIFITFRVQKSPVFRRDGADIHSDLFISIAQALLGGTARAQGLYETINVTIPPGTQTDQKIRMGGKGIPRINSYGYGDHYIHIKIRVPKRLTSRQQSLILSYAEDETDVEGTVNGVTLTSSGGSTMDSSAGSKARREAGEDEEGFLSKLKKMFTS stat1_mouse Signal transducer and activator of transcription 1 Stat1 protein protein mouse Mus musculus Mouse MSQWFELQQLDSKFLEQVHQLYDDSFPMEIRQYLAQWLEKQDWEHAAYDVSFATIRFHDLLSQLDDQYSRFSLENNFLLQHNIRKSKRNLQDNFQEDPVQMSMIIYNCLKEERKILENAQRFNQAQEGNIQNTVMLDKQKELDSKVRNVKDQVMCIEQEIKTLEELQDEYDFKCKTSQNREGEANGVAKSDQKQEQLLLHKMFLMLDNKRKEIIHKIRELLNSIELTQNTLINDELVEWKRRQQSACIGGPPNACLDQLQTWFTIVAETLQQIRQQLKKLEELEQKFTYEPDPITKNKQVLSDRTFLLFQQLIQSSFVVERQPCMPTHPQRPLVLKTGVQFTVKSRLLVKLQESNLLTKVKCHFDKDVNEKNTVKGFRKFNILGTHTKVMNMEESTNGSLAAELRHLQLKEQKNAGNRTNEGPLIVTEELHSLSFETQLCQPGLVIDLETTSLPVVVISNVSQLPSGWASILWYNMLVTEPRNLSFFLNPPCAWWSQLSEVLSWQFSSVTKRGLNADQLSMLGEKLLGPNAGPDGLIPWTRFCKENINDKNFSFWPWIDTILELIKNDLLCLWNDGCIMGFISKERERALLKDQQPGTFLLRFSESSREGAITFTWVERSQNGGEPDFHAVEPYTKKELSAVTFPDIIRNYKVMAAENIPENPLKYLYPNIDKDHAFGKYYSRPKEAPEPMELDDPKRTGYIKTELISVSEVHPSRLQTTDNLLPMSPEEFDEMSRIVGPEFDSMMSTV sta5b_human Signal transducer and activator of transcription 5B STAT5B protein protein human Homo sapiens Human MAVWIQAQQLQGEALHQMQALYGQHFPIEVRHYLSQWIESQAWDSVDLDNPQENIKATQLLEGLVQELQKKAEHQVGEDGFLLKIKLGHYATQLQNTYDRCPMELVRCIRHILYNEQRLVREANNGSSPAGSLADAMSQKHLQINQTFEELRLVTQDTENELKKLQQTQEYFIIQYQESLRIQAQFGPLAQLSPQERLSRETALQQKQVSLEAWLQREAQTLQQYRVELAEKHQKTLQLLRKQQTIILDDELIQWKRRQQLAGNGGPPEGSLDVLQSWCEKLAEIIWQNRQQIRRAEHLCQQLPIPGPVEEMLAEVNATITDIISALVTSTFIIEKQPPQVLKTQTKFAATVRLLVGGKLNVHMNPPQVKATIISEQQAKSLLKNENTRNDYSGEILNNCCVMEYHQATGTLSAHFRNMSLKRIKRSDRRGAESVTEEKFTILFESQFSVGGNELVFQVKTLSLPVVVIVHGSQDNNATATVLWDNAFAEPGRVPFAVPDKVLWPQLCEALNMKFKAEVQSNRGLTKENLVFLAQKLFNNSSSHLEDYSGLSVSWSQFNRENLPGRNYTFWQWFDGVMEVLKKHLKPHWNDGAILGFVNKQQAHDLLINKPDGTFLLRFSDSEIGGITIAWKFDSQERMFWNLMPFTTRDFSIRSLADRLGDLNYLIYVFPDRPKDEVYSKYYTPVPCESATAKAVDGYVKPQIKQVVPEFVNASADAGGGSATYMDQAPSPAVCPQAHYNMYPQNPDSVLDTDGDFDLEDTMDVARRVEELLGRPMDSQWIPHAQS stat1_human Signal transducer and activator of transcription 1-alpha/beta STAT1 Transcription factor ISGF-3 components p91/p84 protein protein human Homo sapiens Human MSQWYELQQLDSKFLEQVHQLYDDSFPMEIRQYLAQWLEKQDWEHAANDVSFATIRFHDLLSQLDDQYSRFSLENNFLLQHNIRKSKRNLQDNFQEDPIQMSMIIYSCLKEERKILENAQRFNQAQSGNIQSTVMLDKQKELDSKVRNVKDKVMCIEHEIKSLEDLQDEYDFKCKTLQNREHETNGVAKSDQKQEQLLLKKMYLMLDNKRKEVVHKIIELLNVTELTQNALINDELVEWKRRQQSACIGGPPNACLDQLQNWFTIVAESLQQVRQQLKKLEELEQKYTYEHDPITKNKQVLWDRTFSLFQQLIQSSFVVERQPCMPTHPQRPLVLKTGVQFTVKLRLLVKLQELNYNLKVKVLFDKDVNERNTVKGFRKFNILGTHTKVMNMEESTNGSLAAEFRHLQLKEQKNAGTRTNEGPLIVTEELHSLSFETQLCQPGLVIDLETTSLPVVVISNVSQLPSGWASILWYNMLVAEPRNLSFFLTPPCARWAQLSEVLSWQFSSVTKRGLNVDQLNMLGEKLLGPNASPDGLIPWTRFCKENINDKNFPFWLWIESILELIKKHLLPLWNDGCIMGFISKERERALLKDQQPGTFLLRFSESSREGAITFTWVERSQNGGEPDFHAVEPYTKKELSAVTFPDIIRNYKVMAAENIPENPLKYLYPNIDKDHAFGKYYSRPKEAPEPMELDGPKGTGYIKTELISVSEVHPSRLQTTDNLLPMSPEEFDEVSRIVGSVEFDSMMNTV dnaja3-stat5b-6 1173254 n/a 1173260 unspecified role unspecified role prey prey n/a 1173261 unspecified role unspecified role bait bait physical association physical association false false false Fig. 2C dnaja3-stat5b-7 1173254 n/a 1173260 unspecified role unspecified role prey prey n/a 1173262 unspecified role unspecified role bait bait physical association physical association false false false Fig. 2C dnaja3-stat5b-11 1173254 n/a 1173263 unspecified role unspecified role bait bait n/a 1173260 unspecified role unspecified role prey prey physical association physical association false false false Fig. 3A dnaja3-stat1-3 1173254 n/a 1173263 unspecified role unspecified role bait bait n/a 1173264 unspecified role unspecified role prey prey physical association physical association false false false Figs. 2D, 3C dnaja3-stat5b-5 1173255 n/a 1173265 unspecified role unspecified role prey prey n/a 1173263 unspecified role unspecified role bait bait physical association physical association false false false Fig. 2B, 3B dnaja3-stat5b-8 1173255 n/a 1173265 unspecified role unspecified role prey prey n/a 1173262 unspecified role unspecified role bait bait physical association physical association false false false Fig. 2C dnaja3-stat5b-9 1173255 n/a 1173261 unspecified role unspecified role bait bait n/a 1173265 unspecified role unspecified role prey prey physical association physical association false false false Fig. 2C dnaja3-stat1-2 1173255 n/a 1173266 unspecified role unspecified role prey prey n/a 1173263 unspecified role unspecified role bait bait physical association physical association false false false Figs. 2D, 3C dnaja3-dnaja3 1173256 n/a 1173260 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1173262 unspecified role unspecified role bait bait physical association physical association false false false Fig. 2A stat5b-dnaja3-2 1173256 n/a 1173261 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1173260 unspecified role unspecified role bait bait physical association physical association false false false Fig. 5B stat5b-dnaja3 1173257 n/a 1173260 unspecified role unspecified role bait bait region activation domain activation domain tag activation domain certain certain sequence position certain certain certain sequence position certain false n/a 1173263 unspecified role unspecified role prey prey physical association physical association false false false stat5b-dnaja3-1 1173258 n/a 1173263 unspecified role unspecified role prey prey region binding region binding-associated region binding region range range range range false n/a 1173260 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false 1173308 1173306 physical association physical association false false false Fig. 1B dnaja3-stat5b-3 1173259 n/a 1173263 unspecified role unspecified role bait bait n/a 1173265 unspecified role unspecified role prey prey physical association physical association false false false Fig. 2B