MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint erdmann-2011-1 Interaction of calmodulin with Sec61α limits Ca2+ leakage from the endoplasmic reticulum. in vitro In vitro fcs fluorescence correlation spectroscopy FCS FCS fcs predetermined predetermined participant predetermined Erdmann F., Schäuble N., Lang S., Jung M., Honigmann A., Ahmad M., Dudek J., Benedix J., Harsman A., Kopp A., Helms V., Cavalié A., Wagner R., Zimmermann R. EMBO J. (0261-4189) 2011 Only protein-protein interactions imex curation erdmann-2011-2 Interaction of calmodulin with Sec61α limits Ca2+ leakage from the endoplasmic reticulum. in vitro In vitro peptide array peptide array predetermined predetermined participant predetermined Erdmann F., Schäuble N., Lang S., Jung M., Honigmann A., Ahmad M., Dudek J., Benedix J., Harsman A., Kopp A., Helms V., Cavalié A., Wagner R., Zimmermann R. EMBO J. (0261-4189) 2011 Only protein-protein interactions imex curation erdmann-2011-3 Interaction of calmodulin with Sec61α limits Ca2+ leakage from the endoplasmic reticulum. in vitro In vitro density sedimentation cosedimentation through density gradient density sedimentation predetermined predetermined participant predetermined Erdmann F., Schäuble N., Lang S., Jung M., Honigmann A., Ahmad M., Dudek J., Benedix J., Harsman A., Kopp A., Helms V., Cavalié A., Wagner R., Zimmermann R. EMBO J. (0261-4189) 2011 Only protein-protein interactions imex curation s61a1_canlf Protein transport protein Sec61 subunit alpha isoform 1 SEC61A1 protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MAIKFLEVIKPFCVILPEIQKPERKIQFKEKVLWTAITLFIFLVCCQIPLFGIMSSDSADPFYWMRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEVGDTPKDRALFNGAQKLFGMIITIGQSIVYVMTGMYGDPSEMGAGICLLITIQLFVAGLIVLLLDELLQKGYGLGSGISLFIATNICETIVWKAFSPTTVNTGRGMEFEGAIIALFHLLATRTDKVRALREAFYRQNLPNLMNLIATIFVFAVVIYFQGFRVDLPIKSARYRGQYNTYPIKLFYTSNIPIILQSALVSNLYVISQMLSARFSGNLLVSLLGTWSDTSSGGPARAYPVGGLCHYLSPPESFGSVLEDPVHAVVYIVFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRETSMVHELNRYIPTAAAFGGLCIGALSVLADFLGAIGSGTGILLAVTIIYQYFEIFVKEQSEVGSMGALLF mint Plays a crucial role in the insertion of secretory and membrane polypeptides into the ER. Required for assembly of membrane and secretory proteins. Tightly associated with membrane-bound ribosomes, either directly or through adaptor proteins calm_rat Calmodulin Calm1 Calm Cam Cam1 Calm2 Cam2 Camb Calm3 Cam3 Camc CaMII protein protein rat Rattus norvegicus (Rat) Rat MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK sc61b_canlf Protein transport protein Sec61 subunit beta SEC61B protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAGRTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS mint Necessary for protein translocation in the endoplasmic reticulum calm1-sec61a1 1016484 s61a1_canfa Sec61 alpha 1016487 peptide synthesis peptide synthesis unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false calm_rat Calmodulin 1016488 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction false false false 1016484 f1d mint calm1-sec61a1-1 1016484 s61a1_canfa Sec61 alpha 1016487 peptide synthesis peptide synthesis unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false calm_rat Calmodulin 1016488 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false 1016484 f1e mint sec61a1-calm1 1016485 s61a1_canfa Sec61 alpha 1016487 peptide synthesis peptide synthesis unspecified role unspecified role bait bait purified purified P38377:p.Arg24Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false calm_rat Calmodulin 1016488 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false f1f mint sec61a1-calm1-1 1016485 s61a1_canfa Sec61 alpha 1016487 peptide synthesis peptide synthesis unspecified role unspecified role bait bait purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P38377:p.Lys29Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false calm_rat Calmodulin 1016488 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false f1f mint calm1-sec61b 1016486 sc61b_canfa Sec61 beta 1016489 western blot western blot Immuno blot Immuno blot unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level calm_rat Calmodulin 1016488 anti tag western anti tag western blot anti tag western unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep over-expressed over expressed level over-expressed gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false mint f2b f2c f2d f2e