UniProt UniProt http://www.uniprot.org kodama-2010-2 Two interacting coiled-coil proteins, WEB1 and PMI2, maintain the chloroplast photorelocation movement velocity in Arabidopsis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kodama Y., Suetsugu N., Kong S.G., Wada M. wadascb@kyushu-u.org Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2010 Only protein-protein interactions imex curation kodama-2010-4 Two interacting coiled-coil proteins, WEB1 and PMI2, maintain the chloroplast photorelocation movement velocity in Arabidopsis. arath-protoplast Arabidopsis thaliana protoplast Protoplast defined as a membrane-bound cell from which the outer wall has been partially or completely removed. bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence Kodama Y., Suetsugu N., Kong S.G., Wada M. wadascb@kyushu-u.org Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2010 Only protein-protein interactions imex curation kodama-2010-1 Two interacting coiled-coil proteins, WEB1 and PMI2, maintain the chloroplast photorelocation movement velocity in Arabidopsis. arath-protoplast Arabidopsis thaliana protoplast Protoplast defined as a membrane-bound cell from which the outer wall has been partially or completely removed. fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Kodama Y., Suetsugu N., Kong S.G., Wada M. wadascb@kyushu-u.org Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2010 Only protein-protein interactions imex curation kodama-2010-3 Two interacting coiled-coil proteins, WEB1 and PMI2, maintain the chloroplast photorelocation movement velocity in Arabidopsis. allce Allium cepa (Onion) Onion fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Kodama Y., Suetsugu N., Kong S.G., Wada M. wadascb@kyushu-u.org Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2010 Only protein-protein interactions imex curation kodama-2010-5 Two interacting coiled-coil proteins, WEB1 and PMI2, maintain the chloroplast photorelocation movement velocity in Arabidopsis. allce Allium cepa (Onion) Onion bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence Kodama Y., Suetsugu N., Kong S.G., Wada M. wadascb@kyushu-u.org Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2010 Only protein-protein interactions imex curation pmi2_arath Protein PLASTID MOVEMENT IMPAIRED 2 PMI2 F4N21.22 At1g66840 WEB2 Protein WEAK CHLOROPLAST MOVEMENT UNDER BLUE LIGHT 2 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGERNLDGTVSVKATINKYGQKATRSVIKSSVAEDLHKSGRELGIYRESRRVAESAKAKAEVELCKAKKIVKELTLRIEESNRRLKSRRIDIEAVMNESRIDGNGGYVRIMRELEDMKQELSKLKLDVVYVSREKVVAEKEVMELESRMEENLKLLESLKLEVDVANEEHVLVEVAKIEALKECKEVEEQREKERKEVSESLHKRKKRIREMIREIERSKNFENELAETLLDIEMLETQLKLVKEMERKVQRNESMSRSKNRAFERGKDNLSVLKEVTEATEAKKAELASINAELFCLVNTMDTLRKEFDHAKKETAWLDKMIQKDDVMLERLNTKLLIAKDQLEAVSKAEERISYLADNLTTSFEKLKSDREAAKKEELKLREEARIINNEIQKTETGFDGKEKELLSKLDELEKAKHAESLALEKLETMVEKTMETREMESRRNSTITISRFEYEYLSGKACHAEETAEKKVEAAMAWVEALKASTKAIMIKTESLKRVSGKTMLEEERESFRMQRSLSIKRLVQDEIQKFKGNSEDNGLINSPKPVRKSVRLSGKFAPVQGGKSRRYSSGNRATPTFFVIKKKKKVPNMVKFFSRKRRNSSLEQ web1_arath Protein WEAK CHLOROPLAST MOVEMENT UNDER BLUE LIGHT 1 At2g26570 WEB1 T9J22.24 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEDLKTVEASDNVVSDNVEKVNPELIDSTIRESNIQSATKVDNIPQSQTDTEETQQSQTDTEETQQSQTDDTTGNAKIYVDDTFSPSDAATAAVLTGKDSTSTTIVEEVMEPDEIGLPSVKITEAATGTARNGGGSPRTVSSPRFSGSPVSTGTPKNVDSHRGLIDTAAPFESVKEAVSKFGGITDWKSHRMQAVERRKLIEEELKKIHEEIPEYKTHSETAEAAKLQVLKELESTKRLIEQLKLNLDKAQTEEQQAKQDSELAKLRVEEMEQGIAEDVSVAAKAQLEVAKARHTTAITELSSVKEELETLHKEYDALVQDKDVAVKKVEEAMLASKEVEKTVEELTIELIATKESLESAHASHLEAEEQRIGAAMARDQDTHRWEKELKQAEEELQRLNQQIHSSKDLKSKLDTASALLLDLKAELVAYMESKLKQEACDSTTNTDPSTENMSHPDLHAAVASAKKELEEVNVNIEKAAAEVSCLKLASSSLQLELEKEKSTLASIKQREGMASIAVASIEAEIDRTRSEIASVQSKEKDAREKMVELPKQLQQAAEEADEAKSLAEVAREELRKAKEEAEQAKAGASTMESRLFAAQKEIEAAKASERLALAAIKALEESESTLKANDTDSPRSVTLSLEEYYELSKRAHEAEELANARVAAAVSRIEEAKETEMRSLEKLEEVNRDMDARKKALKEATEKAEKAKEGKLGVEQELRKWRAEHEQKRKAGDGVNTEKNLKESFEGGKMEQSPEAVVYASSPSESYGTEENSETNLSPQTKSRKKKKKLSFPRFFMFLSKKKSHNN pmi2-o48724 1186117 n/a 1186122 unspecified role unspecified role bait bait n/a 1186123 unspecified role unspecified role prey prey physical association physical association false false false Fig. 2A, S6B o48724-pmi2-1 1186117 n/a 1186123 unspecified role unspecified role bait bait n/a 1186122 unspecified role unspecified role prey prey physical association physical association false false false Fig. 2A, S6B o48724-pmi2-3 1186118 n/a 1186122 unspecified role unspecified role neutral component neutral component region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1186123 unspecified role unspecified role neutral component neutral component region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 2C o48724-o48724 1186118 n/a 1186123 unspecified role unspecified role neutral component neutral component region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1186123 unspecified role unspecified role neutral component neutral component region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 2D, Supp. Fig. 4 o48724-pmi2 1186119 n/a 1186123 unspecified role unspecified role neutral component neutral component region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1186122 unspecified role unspecified role neutral component neutral component region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Fig. 1I o48724-pmi2-2 1186120 n/a 1186123 unspecified role unspecified role neutral component neutral component region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1186122 unspecified role unspecified role neutral component neutral component region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Fig. 2B o48724-pmi2-4 1186121 n/a 1186123 unspecified role unspecified role neutral component neutral component region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1186122 unspecified role unspecified role neutral component neutral component region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. S4A