MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint ren-2010a-1 Polymerization of MIP-1 chemokine (CCL3 and CCL4) and clearance of MIP-1 by insulin-degrading enzyme. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ren M., Guo Q., Guo L., Lenz M., Qian F., Koenen RR., Xu H., Schilling AB., Weber C., Ye RD., Dinner AR., Tang WJ. EMBO J. (0261-4189) 2010 Only protein-protein interactions imex curation ren-2010a-2 Polymerization of MIP-1 chemokine (CCL3 and CCL4) and clearance of MIP-1 by insulin-degrading enzyme. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ren M., Guo Q., Guo L., Lenz M., Qian F., Koenen RR., Xu H., Schilling AB., Weber C., Ye RD., Dinner AR., Tang WJ. EMBO J. (0261-4189) 2010 Only protein-protein interactions imex curation ren-2010a-3 Polymerization of MIP-1 chemokine (CCL3 and CCL4) and clearance of MIP-1 by insulin-degrading enzyme. in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Ren M., Guo Q., Guo L., Lenz M., Qian F., Koenen RR., Xu H., Schilling AB., Weber C., Ye RD., Dinner AR., Tang WJ. EMBO J. (0261-4189) 2010 Only protein-protein interactions imex curation ren-2010a-4 Polymerization of MIP-1 chemokine (CCL3 and CCL4) and clearance of MIP-1 by insulin-degrading enzyme. in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Ren M., Guo Q., Guo L., Lenz M., Qian F., Koenen RR., Xu H., Schilling AB., Weber C., Ye RD., Dinner AR., Tang WJ. EMBO J. (0261-4189) 2010 Only protein-protein interactions imex curation ren-2010a-5 Polymerization of MIP-1 chemokine (CCL3 and CCL4) and clearance of MIP-1 by insulin-degrading enzyme. in vitro In vitro competition binding competition binding predetermined predetermined participant predetermined Ren M., Guo Q., Guo L., Lenz M., Qian F., Koenen RR., Xu H., Schilling AB., Weber C., Ye RD., Dinner AR., Tang WJ. EMBO J. (0261-4189) 2010 Only protein-protein interactions imex curation ccl3_human C-C motif chemokine 3 CCL3 G0S19-1 MIP1A SCYA3 Small-inducible cytokine A3 SIS-beta PAT 464.1 Macrophage inflammatory protein 1-alpha Tonsillar lymphocyte LD78 alpha protein G0/G1 switch regulatory protein 19-1 protein protein human Homo sapiens Human MQVSTAALAVLLCTMALCNQFSASLAADTPTACCFSYTSRQIPQNFIADYFETSSQCSKPGVIFLTKRSRQVCADPSEEWVQKYVSDLELSA mint Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV) ccl4_human C-C motif chemokine 4 CCL4 LAG1 MIP1B SCYA4 G-26 T-lymphocyte-secreted protein HC21 Lymphocyte activation gene 1 protein MIP-1-beta(1-69) Macrophage inflammatory protein 1-beta PAT 744 Protein H400 SIS-gamma Small-inducible cytokine A4 T-cell activation protein 2 protein protein human Homo sapiens Human MKLCVTVLSLLMLVAAFCSPALSAPMGSDPPTACCFSYTARKLPRNFVVDYYETSSLCSQPAVVFQTKRSKQVCADPSESWVQEYVYDLELN ide_human Insulin-degrading enzyme IDE Insulin protease Insulysin Abeta-degrading protease protein protein human Homo sapiens Human MRYRLAWLLHPALPSTFRSVLGARLPPPERLCGFQKKTYSKMNNPAIKRIGNHITKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSAYYSSNLMAVCVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPTKNEFIPTNFEILPLEKEATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL ccl3 1013960 ccl3_human MIP-1A 1013965 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 direct interaction direct interaction false false false mint t1 f1a f1b f1c f1d f1e f1f f1g f2 sf4 st1 ccl4 1013960 ccl4_human MIP-1B 1013966 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 direct interaction direct interaction false false false mint t1 f1c f1d f1e f1f f1g sf4 ccl3-ide 1013960 ccl3_human MIP-1A 1013965 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified ide_human IDE 1013967 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction false false false homomint mint f7a f7b f7c f7d t1 sf2 ccl3-1 1013961 ccl3_human MIP-1A 1013965 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified undefined mutation mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 direct interaction direct interaction false false false mint f3a sf7 ccl4-1 1013961 ccl4_human MIP-1B 1013966 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 direct interaction direct interaction false false false mint f3a sf7 ccl4-1 1013962 ccl4_human MIP-1B 1013966 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 direct interaction direct interaction false false false mint f3b sf6 st2 ccl3-1 1013962 ccl3_human MIP-1A 1013965 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified undefined mutation mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 direct interaction direct interaction false false false mint f3b sf6 st2 ccl3-ide-1 1013963 ccl3_human MIP-1A 1013965 ms participant Identification by mass spectrometry ms participant enzyme target enzyme target substrate substrate neutral component neutral component purified purified ide_human IDE 1013967 predetermined predetermined participant predetermined enzyme enzyme neutral component neutral component purified purified cleavage cleavage reaction cleavage false false false homomint mint f5b sf10 ccl4-ide 1013963 ide_human IDE 1013967 predetermined predetermined participant predetermined enzyme enzyme neutral component neutral component purified purified ccl4_human MIP-1B 1013966 ms participant Identification by mass spectrometry ms participant enzyme target enzyme target substrate substrate neutral component neutral component purified purified cleavage cleavage reaction cleavage false false false homomint mint f5b sf10 ccl3-ide-2 1013964 ccl3_human MIP-1A 1013965 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified ide_human IDE 1013967 predetermined predetermined participant predetermined enzyme target enzyme target substrate substrate neutral component neutral component purified purified direct interaction direct interaction false false false 1013964 f6a homomint mint