UniProt UniProt http://www.uniprot.org srikanth-2010-4 A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells. human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Srikanth S., Jung HJ., Kim KD., Souda P., Whitelegge J., Gwack Y. ygwack@mednet.ucla.edu Nat. Cell Biol. (1465-7392) 2010 mimix curation srikanth-2010-5 A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Srikanth S., Jung HJ., Kim KD., Souda P., Whitelegge J., Gwack Y. ygwack@mednet.ucla.edu Nat. Cell Biol. (1465-7392) 2010 mimix curation srikanth-2010-2 A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Srikanth S., Jung HJ., Kim KD., Souda P., Whitelegge J., Gwack Y. ygwack@mednet.ucla.edu Nat. Cell Biol. (1465-7392) 2010 mimix curation srikanth-2010-1 A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip sequence tag sequence tag identification sequence tag Srikanth S., Jung HJ., Kim KD., Souda P., Whitelegge J., Gwack Y. ygwack@mednet.ucla.edu Nat. Cell Biol. (1465-7392) 2010 mimix curation srikanth-2010-3 A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Srikanth S., Jung HJ., Kim KD., Souda P., Whitelegge J., Gwack Y. ygwack@mednet.ucla.edu Nat. Cell Biol. (1465-7392) 2010 mimix curation srikanth-2010-6 A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells. human Homo sapiens Human fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Srikanth S., Jung HJ., Kim KD., Souda P., Whitelegge J., Gwack Y. ygwack@mednet.ucla.edu Nat. Cell Biol. (1465-7392) 2010 mimix curation orai2_human Protein orai-2 TMEM142B ORAI2 PP1729 C7orf19 CBCIP2 CAP-binding protein complex-interacting protein 2 Transmembrane protein 142B protein protein human Homo sapiens Human MSAELNVPIDPSAPACPEPGHKGMDYRDWVRRSYLELVTSNHHSVQALSWRKLYLSRAKLKASSRTSALLSGFAMVAMVEVQLETQYQYPRPLLIAFSACTTVLVAVHLFALLISTCILPNVEAVSNIHNLNSISESPHERMHPYIELAWGFSTVLGILLFLAEVVLLCWIKFLPVDARRQPGPPPGPGSHTGWQAALVSTIIMVPVGLIFVVFTIHFYRSLVRHKTERHNREIEELHKLKVQLDGHERSLQVL efc4b_human EF-hand calcium-binding domain-containing protein 4B CRACR2A Calcium release-activated calcium channel regulator 2A Calcium release-activated channel regulator 2A EFCAB4B RAB46 Ras-related protein Rab-46 protein protein human Homo sapiens Human MAAPDGRVVSRPQRLGQGSGQGPKGSGACLHPLDSLEQKETQEQTSGQLVMLRKAQEFFQTCDAEGKGFIARKDMQRLHKELPLSLEELEDVFDALDADGNGYLTPQEFTTGFSHFFFSQNNPSQEDAGEQVAQRHEEKVYLSRGDEDLGDMGEDEEAQFRMLMDRLGAQKVLEDESDVKQLWLQLKKEEPHLLSNFEDFLTRIISQLQEAHEEKNELECALKRKIAAYDEEIQHLYEEMEQQIKSEKEQFLLKDTERFQARSQELEQKLLCKEQELEQLTQKQKRLEGQCTALHHDKHETKAENTKLKLTNQELARELERTSWELQDAQQQLESLQQEACKLHQEKEMEVYRVTESLQREKAGLLKQLDFLRERNKHLRDERDICFQKNKAAKANTAASRASWKKRSGSVIGKYVDSRGILRSQSEEEEEVFGIPRRSSLGLSGYPLTEEEPGTGEPGPGGPYPRPLRRIISVEEDPLPQLLDGGFEQPLSKCSEEEEVSDQGVQGQIPEAPPLKLTPTSPRGQPVGKEALCKEESSPSAPDRLFKIVFVGNSAVGKTSFLRRFCEDRFSPGMAATVGIDYRVKTLNVDNSQVALQLWDTAGQERYRCITQQFFRKADGVIVMYDLTDKQSFLSVRRWLSSVEEAVGDRVPVLLLGNKLDNEKEREVPRGLGEQLATENNLIFYECSAYSGHNTKESLLHLARFLKEQEDTVREDTIQVGHPAKKKSCCG orai3_human Protein orai-3 ORAI3 TMEM142C Transmembrane protein 142C protein protein human Homo sapiens Human MKGGEGDAGEQAPLNPEGESPAGSATYREFVHRGYLDLMGASQHSLRALSWRRLYLSRAKLKASSRTSALLSGFAMVAMVEVQLESDHEYPPGLLVAFSACTTVLVAVHLFALMVSTCLLPHIEAVSNIHNLNSVHQSPHQRLHRYVELAWGFSTALGTFLFLAEVVLVGWVKFVPIGAPLDTPTPMVPTSRVPGTLAPVATSLSPASNLPRSSASAAPSQAEPACPPRQACGGGGAHGPGWQAAMASTAIMVPVGLVFVAFALHFYRSLVAHKTDRYKQELEELNRLQGELQAV crcm1_human Calcium release-activated calcium channel protein 1 ORAI1 CRACM1 TMEM142A Protein orai-1 Transmembrane protein 142A protein protein human Homo sapiens Human MHPEPAPPPSRSSPELPPSGGSTTSGSRRSRRRSGDGEPPGAPPPPPSAVTYPDWIGQSYSEVMSLNEHSMQALSWRKLYLSRAKLKASSRTSALLSGFAMVAMVEVQLDADHDYPPGLLIAFSACTTVLVAVHLFALMISTCILPNIEAVSNVHNLNSVKESPHERMHRHIELAWAFSTVIGTLLFLAEVVLLCWVKFLPLKKQPGQPRPTSKPPASGAAANVSTSGITPGQAAAIASTTIMVPFGLIFIVFAVHFYRSLVSHKTDRQFQELNELAEFARLQDQLDHRGDHPLTPGSHYA stim1_human Stromal interaction molecule 1 STIM1 GOK protein protein human Homo sapiens Human MDVCVRLALWLLWGLLLHQGQSLSHSHSEKATGTSSGANSEESTAAEFCRIDKPLCHSEDEKLSFEAVRNIHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVVQWLITYVELPQYEETFRKLQLSGHAMPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDTVLFGPPLLTRHNHLKDFMLVVSIVIGVGGCWFAYIQNRYSKEHMKKMMKDLEGLHRAEQSLHDLQERLHKAQEEHRTVEVEKVHLEKKLRDEINLAKQEAQRLKELREGTENERSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKWLQLTHEVEVQYYNIKKQNAEKQLLVAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILTAKQALSEVTAALRERLHRWQQIEILCGFQIVNNPGIHSLVAALNIDPSWMGSTRPNPAHFIMTDDVDDMDEEIVSPLSMQSPSLQSSVRQRLTEPQHGLGSQRDLTHSDSESSLHMSDRQRVAPKPPQMSRAADEALNAMTSNGSHRLIEGVHPGSLVEKLPDSPALAKKALLALNHGLDKAHSLMELSPSAPPGGSPHLDSSRSHSPSSPDPDTPSPVGDSRALQASRNTRIPHLAGKKAVAEEDNGSIGEETDSSPGRKKFPLKIFKKPLKK orai2-efcab4b 1160444 n/a 1160450 unspecified role unspecified role bait bait n/a 1160451 unspecified role unspecified role prey prey physical association physical association false false false 2c orai3-efcab4b 1160444 n/a 1160452 unspecified role unspecified role bait bait n/a 1160451 unspecified role unspecified role prey prey physical association physical association false false false 2c orai1-efcab4b-4 1160444 n/a 1160451 unspecified role unspecified role prey prey n/a 1160453 unspecified role unspecified role bait bait physical association physical association false false false s7c stim1-orai1 1160445 n/a 1160454 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) n/a 1160454 unspecified role unspecified role prey prey spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm n/a 1160453 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 2d stim1-efcab4b 1160445 n/a 1160454 unspecified role unspecified role bait bait n/a 1160451 unspecified role unspecified role prey prey direct interaction direct interaction false false false 2d orai1-efcab4b-3 1160445 n/a 1160453 unspecified role unspecified role bait bait n/a 1160451 unspecified role unspecified role prey prey direct interaction direct interaction false false false s7b orai1-efcab4b-1 1160446 n/a 1160451 unspecified role unspecified role prey prey n/a 1160453 unspecified role unspecified role bait bait n/a 1160454 unspecified role unspecified role prey prey association association false false false 2a Enhanced upon Ca2+store depletion efcab4b-orai1 1160446 n/a 1160451 unspecified role unspecified role bait bait n/a 1160453 unspecified role unspecified role prey prey n/a 1160454 unspecified role unspecified role prey prey association association false false false 2a Enhanced upon Ca2+ store depletion orai1-efcab4b 1160447 n/a 1160453 unspecified role unspecified role bait bait n/a 1160454 unspecified role unspecified role prey prey n/a 1160451 unspecified role unspecified role prey prey association association false false false 1c Additional proteins were indicated by silver staining, but their identity was not reported (p35, p23) Observed after store depletion of intracellular Ca2+ orai1-efcab4b-2 1160448 n/a 1160451 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) n/a 1160453 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 2b Only observed in absence of Ca2+ efcab4b-stim1 1160449 n/a 1160454 unspecified role unspecified role neutral component neutral component n/a 1160451 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false s9