UniProt UniProt http://www.uniprot.org singh-2008-2 A competition between stimulators and antagonists of Upf complex recruitment governs human nonsense-mediated mRNA decay. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Singh G., Rebbapragada I., Lykke-Andersen J. The used antibody to precipitate the bait protein is not specific and cannot differentiate between eRF3a and eRF3b. eRF3b/GSPT2 (AC Q8IYD1) is indicated here as further experiments with transfected plasmids support its participation. Jens.Lykke-Andersen@colorado.edu PLoS Biol. (1544-9173) 2008 mimix curation singh-2008-3 A competition between stimulators and antagonists of Upf complex recruitment governs human nonsense-mediated mRNA decay. human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Singh G., Rebbapragada I., Lykke-Andersen J. The used antibody to precipitate the bait protein is not specific and cannot differentiate between eRF3a and eRF3b. eRF3b/GSPT2 (AC Q8IYD1) is indicated here as further experiments with transfected plasmids support its participation. Jens.Lykke-Andersen@colorado.edu PLoS Biol. (1544-9173) 2008 mimix curation singh-2008-1 A competition between stimulators and antagonists of Upf complex recruitment governs human nonsense-mediated mRNA decay. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Singh G., Rebbapragada I., Lykke-Andersen J. The used antibody to precipitate the bait protein is not specific and cannot differentiate between eRF3a and eRF3b. eRF3b/GSPT2 (AC Q8IYD1) is indicated here as further experiments with transfected plasmids support its participation. Jens.Lykke-Andersen@colorado.edu PLoS Biol. (1544-9173) 2008 mimix curation singh-2008-4 A competition between stimulators and antagonists of Upf complex recruitment governs human nonsense-mediated mRNA decay. human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Singh G., Rebbapragada I., Lykke-Andersen J. The used antibody to precipitate the bait protein is not specific and cannot differentiate between eRF3a and eRF3b. eRF3b/GSPT2 (AC Q8IYD1) is indicated here as further experiments with transfected plasmids support its participation. Jens.Lykke-Andersen@colorado.edu PLoS Biol. (1544-9173) 2008 mimix curation erf3b_human Eukaryotic peptide chain release factor GTP-binding subunit ERF3B GSPT2 ERF3B G1 to S phase transition protein 2 homolog protein protein human Homo sapiens Human MDSGSSSSDSAPDCWDQVDMESPGSAPSGDGVSSAVAEAQREPLSSAFSRKLNVNAKPFVPNVHAAEFVPSFLRGPTQPPTLPAGSGSNDETCTGAGYPQGKRMGRGAPVEPSREEPLVSLEGSNSAVTMELSEPVVENGEVEMALEESWEHSKEVSEAEPGGGSSGDSGPPEESGQEMMEEKEEIRKSKSVIVPSGAPKKEHVNVVFIGHVDAGKSTIGGQIMFLTGMVDKRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETERKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSIERYEECKEKLVPFLKKVGFSPKKDIHFMPCSGLTGANIKEQSDFCPWYTGLPFIPYLDNLPNFNRSIDGPIRLPIVDKYKDMGTVVLGKLESGSIFKGQQLVMMPNKHNVEVLGILSDDTETDFVAPGENLKIRLKGIEEEEILPGFILCDPSNLCHSGRTFDVQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALISLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLVPEKD rent1_human Regulator of nonsense transcripts 1 UPF1 RENT1 KIAA0221 ATP-dependent helicase RENT1 Nonsense mRNA reducing factor 1 Up-frameshift suppressor 1 homolog protein protein human Homo sapiens Human MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTPPGGPGGPGGGGAGGPGGAGAGAAAGQLDAQVGPEGILQNGAVDDSVAKTSQLLAELNFEEDEEDTYYTKDLPIHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPSATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSGNEDLVIIWLRDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEVTHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSSMYFQTHDQIGMISAGPSHVAAMNIPIPFNLVMPPMPPPGYFGQANGPAAGRGTPKGKTGRGGRQKNRFGLPGPSQTNLPNSQASQDVASQPFSQGALTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY pabp1_human Polyadenylate-binding protein 1 PABPC1 PAB1 PABP1 PABPC2 PABP protein protein human Homo sapiens Human MNPSAPSYPMASLYVGDLHPDVTEAMLYEKFSPAGPILSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGSKGYGFVHFETQEAAERAIEKMNGMLLNDRKVFVGRFKSRKEREAELGARAKEFTNVYIKNFGEDMDDERLKDLFGKFGPALSVKVMTDESGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKRKFEQMKQDRITRYQGVNLYVKNLDDGIDDERLRKEFSPFGTITSAKVMMEGGRSKGFGFVCFSSPEEATKAVTEMNGRIVATKPLYVALAQRKEERQAHLTNQYMQRMASVRAVPNPVINPYQPAPPSGYFMAAIPQTQNRAAYYPPSQIAQLRPSPRWTAQGARPHPFQNMPGAIRPAAPRPPFSTMRPASSQVPRVMSTQRVANTSTQTMGPRPAAAAAAATPAVRTVPQYKYAAGVRNPQQHLNAQPQVTMQQPAVHVQGQEPLTASMLASAPPQEQKQMLGERLFPLIQAMHPTLAGKITGMLLEIDNSELLHMLESPESLRSKVDEAVAVLQAHQAKEAAQKAVNSATGVPTV erf1_human Eukaryotic peptide chain release factor subunit 1 ETF1 ERF1 Protein Cl1 RF1 SUP45L1 TB3-1 protein protein human Homo sapiens Human MADDPSAADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDDSKFGFIVIDGSGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAETAVQLFISGDKVNVAGLVLAGSADFKTELSQSDMFDQRLQSKVLKLVDISYGGENGFNQAIELSTEVLSNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLKALEMGAVEILIVYENLDIMRYVLHCQGTEEEKILYLTPEQEKDKSHFTDKETGQEHELIESMPLLEWFANNYKKFGATLEIVTDKSQEGSQFVKGFGGIGGILRYRVDFQGMEYQGGDDEFFDLDDY erf3b-upf1 1135768 n/a 1135772 unspecified role unspecified role bait bait n/a 1135773 unspecified role unspecified role prey prey physical association physical association false false false 6B erf3b-pabpc1-1 1135768 n/a 1135772 unspecified role unspecified role bait bait n/a 1135774 unspecified role unspecified role prey prey physical association physical association false false false 6B pabpc1-erf3b 1135769 n/a 1135772 unspecified role unspecified role prey prey n/a 1135774 unspecified role unspecified role bait bait physical association physical association false false false 6C upf1-erf3b 1135769 n/a 1135772 unspecified role unspecified role prey prey n/a 1135773 unspecified role unspecified role bait bait physical association physical association false false false 6c erf3b-pabpc1 1135770 n/a 1135772 unspecified role unspecified role bait bait n/a 1135773 unspecified role unspecified role prey prey n/a 1135774 unspecified role unspecified role prey prey association association false false false 6A erf3b-etf1 1135771 n/a 1135772 unspecified role unspecified role bait bait n/a 1135775 unspecified role unspecified role prey prey physical association physical association false false false 6C