UniProt UniProt http://www.uniprot.org kosoy-2007-1 Fission yeast Rnf4 homologs are required for DNA repair. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Kosoy A., Calonge TM., Outwin EA., O'Connell MJ. matthew.oconnell@mssm.edu S. pombe cDNA J. Biol. Chem. (0021-9258) 2007 Only protein-protein interactions imex curation kosoy-2007-2 Fission yeast Rnf4 homologs are required for DNA repair. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kosoy A., Calonge TM., Outwin EA., O'Connell MJ. matthew.oconnell@mssm.edu S. pombe cDNA J. Biol. Chem. (0021-9258) 2007 Only protein-protein interactions imex curation kosoy-2007-3 Fission yeast Rnf4 homologs are required for DNA repair. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kosoy A., Calonge TM., Outwin EA., O'Connell MJ. matthew.oconnell@mssm.edu S. pombe cDNA J. Biol. Chem. (0021-9258) 2007 Only protein-protein interactions imex curation rad24_schpo Checkpoint signal transducer rad24 rad24 SPAC8E11.02c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSTTSREDAVYLAKLAEQAERYEGMVENMKSVASTDQELTVEERNLLSVAYKNVIGARRASWRIVSSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTVLEKHLIPNAASAESKVFYYKMKGDYYRYLAEFAVGEKRQHSADQSLEGYKAASEIATAELAPTHPIRLGLALNFSVFYYEILNSPDRACYLAKQAFDEAISELDSLSEESYKDSTLIMQLLRDNLTLWTSDAEYSAAAAGGNTEGAQENAPSNAPEGEAEPKADA chk1_schpo Serine/threonine-protein kinase chk1 Checkpoint kinase 1 chk1 rad27 SPCC1259.13 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MAQKLDNFPYHIGREIGTGAFASVRLCYDDNAKIYAVKFVNKKHATSCMNAGVWARRMASEIQLHKLCNGHKNIIHFYNTAENPQWRWVVLEFAQGGDLFDKIEPDVGIDEDVAQFYFAQLMEGISFMHSKGVAHRDLKPENILLDYNGNLKISDFGFASLFSYKGKSRLLNSPVGSPPYAAPEITQQYDGSKVDVWSCGIILFALLLGNTPWDEAISNTGDYLLYKKQCERPSYHPWNLLSPGAYSIITGMLRSDPFKRYSVKHVVQHPWLTSSTPFRTKNGNCADPVALASRLMLKLRIDLDKPRLASSRASQNDSGFSMTQPAFKKNDQKELDRVEVYGALSQPVQLNKNIDVTEILEKDPSLSQFCENEGFIKRLAKKAKNFYEICPPERLTRFYSRASRETIIDHLYDSLRLLAISVTMKYVRNQTILYVNLHDKRKCLLQGVIELTNLGHNLELINFIKRNGDPLEWRKFFKNVVSSIGKPIVLTDVSQN ycs3_schpo Uncharacterized calcium-binding protein C613.03 SPCC613.03 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MKFSTVGFLFSTILFKSAFAGWMDTHMKDEHHIDKYTDESFFRLHDLGKKGYWSDQDILSLYGLFENDEVPFVKKNEVLVDVLKKCDPSGNRRITLDEFLAFRKNGGELTDFGFPGHHGDEEEEFEMHHVEKYHPAGLDEPDENWNHPEDIEHFQKHDEIFHGDKKPEERRKHFVKYNNIPDKYRRVSI ded1_schpo ATP-dependent RNA helicase ded1 ded1 dep1 moc2 sum3 Multicopy suppressor of overexpressed cyr1 protein 2 SPCC1795.11 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSDNVQQQVDSVGSVTEKLQKTNISRPRKYIPPFARDKPSAGAAPAVGDDESVSSRGSSRSQTPSEFSSNYGGRREYNRGGHYGGGEGRQNNYRGGREGGYSNGGGYRNNRGFGQWRDGQHVIGARNTLLERQLFGAVADGTKVSTGINFEKYDDIPVEVSGGDIEPVNEFTSPPLNSHLLQNIKLSGYTQPTPVQKNSIPIVTSGRDLMACAQTGSGKTAGFLFPILSLAFDKGPAAVPVDQDAGMGYRPRKAYPTTLILAPTRELVCQIHEESRKFCYRSWVRPCAVYGGADIRAQIRQIDQGCDLLSATPGRLVDLIDRGRISLANIKFLVLDEADRMLDMGFEPQIRHIVEGADMTSVEERQTLMFSATFPRDIQLLARDFLKDYVFLSVGRVGSTSENITQKVVHVEDSEKRSYLLDILHTLPPEGLTLIFVETKRMADTLTDYLLNSNFPATSIHGDRTQRERERALELFRSGRTSIMVATAVASRGLDIPNVTHVINYDLPTDIDDYVHRIGRTGRAGNTGQAVAFFNRNNKGIAKELIELLQEANQECPSFLIAMARESSFGGNGRGGRYSGRGGRGGNAYGARDFRRPTNSSSGYSSGPSYSGYGGFESRTPHHGNTYNSGSAQSWW rfp1_schpo E3 ubiquitin-protein ligase complex slx8-rfp subunit rfp1 RING-type E3 ubiquitin transferase rfp1 rfp1 mug140 Meiotically up-regulated gene 140 protein RING finger protein 1 SPAC19A8.10 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MQFNGSNGIDESSVIDLTRSPSPPVETSISSTNIIDLDAIPDDSFPSSPVLSPRRRRMNRRRNERSRNFPSNHLSYLEDMIYLGPQVSTRRSSSRRDLMGMIARTFPEFSSVNSLSPSLFQLIVNRMRFDAIHPEWTNGSDDEYFSNHFEESYDDFTSSLENIKQSYKPPGPPKSGFTRSFNNDTLMVCPRCQEPLGTSKSKEKSALWATKCGHVYCGSCAKVLKTSKRSQSKCLVNDCGRYLNTKNAMWELFY cek1_schpo Serine/threonine-protein kinase cek1 cek1 SPCC1450.11c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MKHIKNEREEVFLEDDQAQHSQAELLSSKDENLQPSIPLSPVAFELDFSGNFQFISDNSSELLDIPKDKIIGHSVAEVLGTDGYNAFMRAVNCLLKDDSHSYHVRFQHSINANHANQNYYTAKGDLPSDEKITKPFDAIGILIRHPGSAIPAHTMWVVNPATNSLGSVSPLVTKLLDVIGFGASLLDKYLCDLRTSYHKHNSLDALPLPTPEFCQICEREIQSWFFELHSKFCLSTSTYESVVQAAQDSLLYFRSTLLEIQEGMQKDSSLVPVYKNEPLIVDADDYFFTDENKQTLSLCSFLSQVMYYLEVAIDITIPPVKIIVNFDKVDSLRVQSPRSEKATIELDNYNPSLENCSSAVIALWEDIKTAVDTKITGVLRLRNAIYYSERIRLEIDHHVQEIIDDVVSNLVTNHSSTSLGHLESKLAPSITFPDACDALEAEECITRPGSATNTPQSDRSLDINDLSRSSSYSRHLSHVSLSNPDFAIGSPMSQDSSNYSSPLHRRKASDSNFSDPRFDDLKYLSPNSSPRFVASDGPNRPASNGRSSLFSRGRASNLGDVGLRLPSPSPRIHTIVPNSAPEHPSINDYKILKPISKGAFGSVYLAQKRTTGDYFAIKILKKSNMIAKNQVINVRAERAILMSQGESPFVAKLYYTFQSKDYLYLVMEYLNGGDCGSLLKTMGVLDLDWIRTYIAETVLCLGDLHDRGIIHRDIKPENLLISQNGHLKLTDFGLSRVGYMKRHRRKQSSSIPVLDLRDRSSAISDLSLSTASSVLEAQSLITPERPKRPSLNEKLLSLDGTSIRLAGQSFNYENSAEDSPTATNTPTSQVDESNIFRSTDSPRVQPFFENKDPSKRFIGTPDYIAPEVILGNPGIKASDWWSLGCVVFEFLFGYPPFNAETPDQVFQNILARRINWPAEVFTAESSVALDLIDRLLCMNPANRLGANGVEEIKAHPFFKSVNWDTILEEDPPFVPKPFSPEDTVYFDSRGLKGFDFSEYYNQPTVTEAQKLEEERPASSIPQHVSGNRKGRLRSNTISTPEFGSFTYRNLDFLNKANRNTIQKLRKEHMAVKSAKTSVDDTFSQYMSRFKAKLSTSQSVGPVKSSRRASMADYEASTTTRVQDITTDSIDSIDDFDSLKEGRMLSFFDNLALEDHKGVSSTMSASQSQSSMHTALPDVTEGTSSDEHTTIQKGRIDNLQAQSLTHKRNAISYPGLFQLDRLQMIIPKDEIELAEILKKIFPKLTLVLIDDPWSILKKLLQNEQFNVVFLHFGNDKVSSSRLMYSVRTSATINSRVPFVYICEDETCIPTDLQSDGVLLKPITCENIESCLRKLDVWHS dcp2_schpo mRNA decapping complex subunit 2 dcp2 SPAC19A8.12 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSFTNATFSQVLDDLSARFILNLPAEEQSSVERLCFQIEQAHWFYEDFIRAQNDQLPSLGLRVFSAKLFAHCPLLWKWSKVHEEAFDDFLRYKTRIPVRGAIMLDMSMQQCVLVKGWKASSGWGFPKGKIDKDESDVDCAIREVYEETGFDCSSRINPNEFIDMTIRGQNVRLYIIPGISLDTRFESRTRKEISKIEWHNLMDLPTFKKNKPQTMKNKFYMVIPFLAPLKKWIKKRNIANNTTKEKNISVDVDADASSQLLSLLKSSTAPSDLATPQPSTFPQPPVESHSSFDIKQKILHLLNEGNEPKSPIQLPPVSNLPLNPPIQSSNSRLSHDNNSFDPFAYLGLDPKNPSASFPRVVSQNNMLTNKPVLNNHFQQSMYSNLLKDQNSVQHLFAASDMPSPMELPSPSTVYHQVFYPPTSTSVSSYGLGKTPQPAYGSSSPYVNGHQTQQISSLPPFQSQTQFLARNSDNSGQSYNSEGDSNSKRLLSMLSQQDTTPSSSTLSKEANVQLANLFLTPNSLETKKFSDNSQGEEISDNLHGESCNNPNANSVHSAQLLQALLHPSATETKEETPKKTSDSLSLLTLLKSGLPTPANDLQNKSQNNERKASSQVKELEVKNYSKSTDLLKKTLRIPRNDEPLEAANQFDLLKVSPQQKSEVPPKRNELSQSKLKNRKKKENSETNKNHVDMSPGFVKILKRSPLADQKKEDTQESDFKGSDDHFLSYLQSVVSSNSNGLH B405257B706BC5C0 chk1-rad24 1021441 n/a 1021444 unspecified role unspecified role prey prey n/a 1021445 unspecified role unspecified role bait bait physical association physical association false false false Table 1 chk1-ycs3 1021441 n/a 1021446 unspecified role unspecified role prey prey n/a 1021445 unspecified role unspecified role bait bait physical association physical association false false false Table 1 chk1-ded1 1021441 n/a 1021445 unspecified role unspecified role bait bait n/a 1021447 unspecified role unspecified role prey prey physical association physical association false false false Table 1 chk1-rfp1 1021441 n/a 1021448 unspecified role unspecified role prey prey n/a 1021445 unspecified role unspecified role bait bait physical association physical association false false false Table 1 chk1-cek1 1021441 n/a 1021449 unspecified role unspecified role prey prey n/a 1021445 unspecified role unspecified role bait bait physical association physical association false false false Table 1 chk1-rfp1-2 1021441 n/a 1021450 unspecified role unspecified role bait bait n/a 1021445 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1B chk1-rfp1-1 1021442 n/a 1021448 unspecified role unspecified role prey prey n/a 1021445 unspecified role unspecified role bait bait c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 1A chk1-rfp1-2 1021443 n/a 1021448 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1021445 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 1B