<?xml version="1.0" encoding="UTF-8" standalone="yes"?>
<entrySet level="2" version="5" minorVersion="4" xsi:schemaLocation="http://psi.hupo.org/mi/mif http://psidev.sourceforge.net/mi/rel25/src/MIF254.xsd" xmlns="http://psi.hupo.org/mi/mif" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
    <entry>
        <source releaseDate="2011-04-19+01:00">
            <names>
                <shortLabel>UniProt</shortLabel>
                <fullName>UniProt</fullName>
            </names>
            <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0486" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-3989247" refType="identity" refTypeAc="MI:0356"/>
            </xref>
            <attributeList>
                <attribute name="url" nameAc="MI:0614">http://www.uniprot.org</attribute>
            </attributeList>
        </source>
        <experimentList>
            <experimentDescription id="1131028">
                <names>
                    <shortLabel>dong-2006-1</shortLabel>
                    <fullName>Crystal structure of tRNA pseudouridine synthase TruA from Thermus thermophilus HB8.</fullName>
                </names>
                <bibref>
                    <xref>
                        <primaryRef db="pubmed" dbAc="MI:0446" id="17114947" refType="primary-reference" refTypeAc="MI:0358"/>
                    </xref>
                </bibref>
                <xref>
                    <primaryRef db="intact" dbAc="MI:0469" id="EBI-3843457" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="imex" dbAc="MI:0670" id="IM-16975" refType="imex-primary" refTypeAc="MI:0662"/>
                </xref>
                <hostOrganismList>
                    <hostOrganism ncbiTaxId="-1">
                        <names>
                            <shortLabel>in vitro</shortLabel>
                            <fullName>In vitro</fullName>
                        </names>
                    </hostOrganism>
                </hostOrganismList>
                <interactionDetectionMethod>
                    <names>
                        <shortLabel>x-ray diffraction</shortLabel>
                        <fullName>x-ray crystallography</fullName>
                        <alias typeAc="MI:0303" type="go synonym">X-ray</alias>
                        <alias typeAc="MI:1041" type="synonym">X-ray</alias>
                        <alias typeAc="MI:1041" type="synonym">x-ray diffraction</alias>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0114" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1272" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                    </xref>
                </interactionDetectionMethod>
                <participantIdentificationMethod>
                    <names>
                        <shortLabel>predetermined</shortLabel>
                        <fullName>predetermined participant</fullName>
                        <alias typeAc="MI:1041" type="synonym">predetermined</alias>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                    </xref>
                </participantIdentificationMethod>
                <attributeList>
                    <attribute name="author-list" nameAc="MI:0636">Dong X., Bessho Y., Shibata R., Nishimoto M., Shirouzu M., Kuramitsu S., Yokoyama S.</attribute>
                    <attribute name="contact-email" nameAc="MI:0634">yokoyama@biochem.s.u-tokyo.ac.jp</attribute>
                    <attribute name="journal" nameAc="MI:0885">RNA Biol (1547-6286)</attribute>
                    <attribute name="publication year" nameAc="MI:0886">2006</attribute>
                    <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
                    <attribute name="imex curation" nameAc="MI:0959"/>
                    <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
                </attributeList>
            </experimentDescription>
        </experimentList>
        <interactorList>
            <interactor id="1131029">
                <names>
                    <shortLabel>trua_thet8</shortLabel>
                    <fullName>tRNA pseudouridine synthase A</fullName>
                    <alias typeAc="MI:0302" type="gene name synonym">tRNA pseudouridine(38-40) synthase</alias>
                    <alias typeAc="MI:0301" type="gene name">truA</alias>
                    <alias typeAc="MI:0302" type="gene name synonym">tRNA pseudouridylate synthase I</alias>
                    <alias typeAc="MI:0302" type="gene name synonym">tRNA-uridine isomerase I</alias>
                    <alias typeAc="MI:0305" type="locus name">TTHA1631</alias>
                </names>
                <xref>
                    <primaryRef db="uniprotkb" dbAc="MI:0486" id="Q5SHU9" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1VS3"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR020095"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR001406"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR020097"/>
                    <secondaryRef db="ensemblbacteria" id="BAD71454" version="SP_102" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="ensemblbacteria" id="BAD71454" version="SP_102" refType="transcript"/>
                    <secondaryRef db="ensemblbacteria" id="BAD71454" version="SP_102" refType="gene"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-3843473" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0003723" secondary="RNA binding" version="SP_102"/>
                    <secondaryRef db="refseq" dbAc="MI:0481" id="WP_011173668.1"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0106029" secondary="tRNA pseudouridine synthase activity" version="SP_102"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0031119" secondary="tRNA pseudouridine synthesis" version="SP_102"/>
                    <secondaryRef db="refseq" dbAc="MI:0481" id="YP_144897.1"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR020094" version="SP_102"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR020103"/>
                </xref>
                <interactorType>
                    <names>
                        <shortLabel>protein</shortLabel>
                        <fullName>protein</fullName>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
                    </xref>
                </interactorType>
                <organism ncbiTaxId="300852">
                    <names>
                        <shortLabel>thet8</shortLabel>
                        <fullName>Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)</fullName>
                    </names>
                </organism>
                <sequence>MRRLLLLCEYDGTLFAGLQRQGRGLRTVQGELERALPGIGALPKAVAAGRTDAGVHALAMPFHVDVESAIPVEKVPEALNRLLPEDLKVVGAREVAPDFHARKDALWRAYRYRILVRPHPSPLLRHRALWVRRPLDLEAMEEALSLLLGRHNFLGFAKEETRPGERELLEARLQVAEGEAGLEVRLYFRGKSFLRGQVRGMVGTLLEVGLGKRPPESLKAILKTADRRLAGPTAPAHGLYFVEAAYPEEKLSP</sequence>
            </interactor>
        </interactorList>
        <interactionList>
            <interaction imexId="IM-16975-1" id="1131030">
                <names>
                    <shortLabel>dim-2</shortLabel>
                </names>
                <xref>
                    <primaryRef db="pdbe" dbAc="MI:0472" id="1vs3" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-3843472" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="imex" dbAc="MI:0670" id="IM-16975-1" refType="imex-primary" refTypeAc="MI:0662"/>
                </xref>
                <experimentList>
                    <experimentRef>1131028</experimentRef>
                </experimentList>
                <participantList>
                    <participant id="1131031">
                        <names>
                            <shortLabel>n/a</shortLabel>
                        </names>
                        <xref>
                            <primaryRef db="intact" dbAc="MI:0469" id="EBI-3843476" refType="identity" refTypeAc="MI:0356"/>
                        </xref>
                        <interactorRef>1131029</interactorRef>
                        <biologicalRole>
                            <names>
                                <shortLabel>unspecified role</shortLabel>
                                <fullName>unspecified role</fullName>
                            </names>
                            <xref>
                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                            </xref>
                        </biologicalRole>
                        <experimentalRoleList>
                            <experimentalRole>
                                <names>
                                    <shortLabel>neutral component</shortLabel>
                                    <fullName>neutral component</fullName>
                                </names>
                                <xref>
                                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-55" refType="identity" refTypeAc="MI:0356"/>
                                </xref>
                            </experimentalRole>
                        </experimentalRoleList>
                        <featureList>
                            <feature id="1131032">
                                <names>
                                    <shortLabel>region</shortLabel>
                                </names>
                                <xref>
                                    <primaryRef db="intact" dbAc="MI:0469" id="EBI-3843500" refType="identity" refTypeAc="MI:0356"/>
                                </xref>
                                <featureType>
                                    <names>
                                        <shortLabel>sufficient to bind</shortLabel>
                                        <fullName>sufficient binding region</fullName>
                                        <alias typeAc="MI:1041" type="synonym">sufficient to bind</alias>
                                    </names>
                                    <xref>
                                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0442" refType="identity" refTypeAc="MI:0356"/>
                                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608899" refType="identity" refTypeAc="MI:0356"/>
                                    </xref>
                                </featureType>
                                <featureDetectionMethod>
                                    <names>
                                        <shortLabel>x-ray diffraction</shortLabel>
                                        <fullName>x-ray crystallography</fullName>
                                        <alias typeAc="MI:0303" type="go synonym">X-ray</alias>
                                        <alias typeAc="MI:1041" type="synonym">x-ray diffraction</alias>
                                        <alias typeAc="MI:1041" type="synonym">X-ray</alias>
                                    </names>
                                    <xref>
                                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0114" refType="identity" refTypeAc="MI:0356"/>
                                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-456835" refType="identity" refTypeAc="MI:0356"/>
                                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                    </xref>
                                </featureDetectionMethod>
                                <featureRangeList>
                                    <featureRange>
                                        <startStatus>
                                            <names>
                                                <shortLabel>certain</shortLabel>
                                                <fullName>certain sequence position</fullName>
                                                <alias typeAc="MI:1041" type="synonym">certain</alias>
                                            </names>
                                            <xref>
                                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                            </xref>
                                        </startStatus>
                                        <begin position="1"/>
                                        <endStatus>
                                            <names>
                                                <shortLabel>certain</shortLabel>
                                                <fullName>certain sequence position</fullName>
                                                <alias typeAc="MI:1041" type="synonym">certain</alias>
                                            </names>
                                            <xref>
                                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                            </xref>
                                        </endStatus>
                                        <end position="249"/>
                                        <isLink>false</isLink>
                                    </featureRange>
                                </featureRangeList>
                            </feature>
                        </featureList>
                        <hostOrganismList>
                            <hostOrganism ncbiTaxId="83333">
                                <names>
                                    <shortLabel>ecoli</shortLabel>
                                    <fullName>Escherichia coli (strain K12)</fullName>
                                </names>
                            </hostOrganism>
                        </hostOrganismList>
                        <attributeList>
                            <attribute name="comment" nameAc="MI:0612">Stoichiometry: 2.0</attribute>
                        </attributeList>
                    </participant>
                </participantList>
                <interactionType>
                    <names>
                        <shortLabel>direct interaction</shortLabel>
                        <fullName>direct interaction</fullName>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0407" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608833" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                    </xref>
                </interactionType>
                <modelled>false</modelled>
                <intraMolecular>false</intraMolecular>
                <negative>false</negative>
                <attributeList>
                    <attribute name="3d-resolution" nameAc="MI:0632">2.25 A 0.234</attribute>
                    <attribute name="3d-r-factors" nameAc="MI:0631">R-value 0.222  R-free</attribute>
                </attributeList>
            </interaction>
        </interactionList>
    </entry>
</entrySet>
