UniProt UniProt http://www.uniprot.org stankovic_valentin-2006-2 A L225A substitution in the human tumour suppressor HIC1 abolishes its interaction with the corepressor CtBP. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Stankovic-Valentin N., Verger A., Deltour-Balerdi S., Quinlan K.G., Crossley M., Leprince D. Origin of FLAG-HIC1 according pmid 12052894; however, isoform identity cannot be established. Origin of all transfected CtBP1 and CtBP2 constructs as mouse as indicated in the paper for some experiments. dominique.leprince@ibl.fr FEBS J. (1742-464X) 2006 mimix curation stankovic_valentin-2006-1 A L225A substitution in the human tumour suppressor HIC1 abolishes its interaction with the corepressor CtBP. human-daoy Homo sapiens DAOY cerebellar medulloblastoma cell line daoy DAOY cerebellar medulloblastoma cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Stankovic-Valentin N., Verger A., Deltour-Balerdi S., Quinlan K.G., Crossley M., Leprince D. Origin of FLAG-HIC1 according pmid 12052894; however, isoform identity cannot be established. Origin of all transfected CtBP1 and CtBP2 constructs as mouse as indicated in the paper for some experiments. dominique.leprince@ibl.fr FEBS J. (1742-464X) 2006 mimix curation stankovic_valentin-2006-3 A L225A substitution in the human tumour suppressor HIC1 abolishes its interaction with the corepressor CtBP. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Stankovic-Valentin N., Verger A., Deltour-Balerdi S., Quinlan K.G., Crossley M., Leprince D. Origin of FLAG-HIC1 according pmid 12052894; however, isoform identity cannot be established. Origin of all transfected CtBP1 and CtBP2 constructs as mouse as indicated in the paper for some experiments. dominique.leprince@ibl.fr FEBS J. (1742-464X) 2006 mimix curation stankovic_valentin-2006-4 A L225A substitution in the human tumour suppressor HIC1 abolishes its interaction with the corepressor CtBP. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Stankovic-Valentin N., Verger A., Deltour-Balerdi S., Quinlan K.G., Crossley M., Leprince D. Origin of FLAG-HIC1 according pmid 12052894; however, isoform identity cannot be established. Origin of all transfected CtBP1 and CtBP2 constructs as mouse as indicated in the paper for some experiments. dominique.leprince@ibl.fr FEBS J. (1742-464X) 2006 mimix curation stankovic_valentin-2006-5 A L225A substitution in the human tumour suppressor HIC1 abolishes its interaction with the corepressor CtBP. rabit-rk13 rk13 cell line originating from rabbit kidney epithelial cells rk13 RK13 cell line produced from rabbit kidney epithelial cells 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Stankovic-Valentin N., Verger A., Deltour-Balerdi S., Quinlan K.G., Crossley M., Leprince D. Origin of FLAG-HIC1 according pmid 12052894; however, isoform identity cannot be established. Origin of all transfected CtBP1 and CtBP2 constructs as mouse as indicated in the paper for some experiments. dominique.leprince@ibl.fr FEBS J. (1742-464X) 2006 mimix curation hic1_human Hypermethylated in cancer 1 protein HIC1 ZBTB29 Zinc finger and BTB domain-containing protein 29 protein protein human Homo sapiens Human MTFPEADILLKSGECAGQTMLDTMEAPGHSRQLLLQLNNQRTKGFLCDVIIVVQNALFRAHKNVLAASSAYLKSLVVHDNLLNLDHDMVSPAVFRLVLDFIYTGRLADGAEAAAAAAVAPGAEPSLGAVLAAASYLQIPDLVALCKKRLKRHGKYCHLRGGGGGGGGYAPYGRPGRGLRAATPVIQACYPSPVGPPPPPAAEPPSGPEAAVNTHCAELYASGPGPAAALCASERRCSPLCGLDLSKKSPPGSAAPERPLAERELPPRPDSPPSAGPAAYKEPPLALPSLPPLPFQKLEEAAPPSDPFRGGSGSPGPEPPGRPDGPSLLYRWMKHEPGLGSYGDELGRERGSPSERCEERGGDAAVSPGGPPLGLAPPPRYPGSLDGPGAGGDGDDYKSSSEETGSSEDPSPPGGHLEGYPCPHLAYGEPESFGDNLYVCIPCGKGFPSSEQLNAHVEAHVEEEEALYGRAEAAEVAAGAAGLGPPFGGGGDKVAGAPGGLGELLRPYRCASCDKSYKDPATLRQHEKTHWLTRPYPCTICGKKFTQRGTMTRHMRSHLGLKPFACDACGMRFTRQYRLTEHMRIHSGEKPYECQVCGGKFAQQRNLISHMKMHAVGGAAGAAGALAGLGGLPGVPGPDGKGKLDFPEGVFAVARLTAEQLSLKQQDKAAAAELLAQTTHFLHDPKVALESLYPLAKFTAELGLSPDKAAEVLSQGAHLAAGPDGRTIDRFSPT ctbp1_mouse C-terminal-binding protein 1 Ctbp1 protein protein mouse Mus musculus Mouse MGSSHLLNKGLPLGVRPPIMNGPMHPRPLVALLDGRDCTVEMPILKDVATVAFCDAQSTQEIHEKVLNEAVGALMYHTITLTREDLEKFKALRIIVRIGSGFDNIDIKSAGDLGIAVCNVPAASVEETADSTLCHILNLYRRTTWLHQALREGTRVQSVEQIREVASGAARIRGETLGIIGLGRVGQAVALRAKAFGFNVLFYDPYLSDGIERALGLQRVSTLQDLLFHSDCVTLHCGLNEHNHHLINDFTVKQMRQGAFLVNTARGGLVDEKALAQALKEGRIRGAALDVHESEPFSFSQGPLKDAPNLICTPHAAWYSEQASIEMREEAAREIRRAITGRIPDSLKNCVNKDHLTAATHWASMDPAVVHPELNGAAYSRYPPGVVSVAPTGIPAAVEGIVPSAMSLSHGLPPVAHPPHAPSPGQTVKPEADRDHTSDQL ctbp2_mouse C-terminal-binding protein 2 Ctbp2 protein protein mouse Mus musculus Mouse MALVDKHKVKRQRLDRICEGIRPQIMNGPLHPRPLVALLDGRDCTVEMPILKDLATVAFCDAQSTQEIHEKVLNEAVGAMMYHTITLTREDLEKFKALRVIVRIGSGYDNVDIKAAGELGIAVCNIPSAAVEETADSTVCHILNLYRRNTWLYQALREGTRVQSVEQIREVASGAARIRGETLGLIGFGRTGQAVAVRAKAFGFSVIFYDPYLQDGIERSLGVQRVYTLQDLLYQSDCVSLHCNLNEHNHHLINDFTIKQMRQGAFLVNAARGGLVDEKALAQALKEGRIRGAALDVHESEPFSFAQGPLKDAPNLICTPHTAWYSEQASLEMREAAATEIRRAITGRIPESLRNCVNKEFFVTSAPWSVIDQQAIHPELNGATYRYPPGIVGVAPGGLPPAMEGIIPGGIPVTHNLPTVAHPSQAPSPNQPTKHGDNREHPNEQ ctbp1_human C-terminal-binding protein 1 CTBP1 CTBP protein protein human Homo sapiens Human MGSSHLLNKGLPLGVRPPIMNGPLHPRPLVALLDGRDCTVEMPILKDVATVAFCDAQSTQEIHEKVLNEAVGALMYHTITLTREDLEKFKALRIIVRIGSGFDNIDIKSAGDLGIAVCNVPAASVEETADSTLCHILNLYRRATWLHQALREGTRVQSVEQIREVASGAARIRGETLGIIGLGRVGQAVALRAKAFGFNVLFYDPYLSDGVERALGLQRVSTLQDLLFHSDCVTLHCGLNEHNHHLINDFTVKQMRQGAFLVNTARGGLVDEKALAQALKEGRIRGAALDVHESEPFSFSQGPLKDAPNLICTPHAAWYSEQASIEMREEAAREIRRAITGRIPDSLKNCVNKDHLTAATHWASMDPAVVHPELNGAAYRYPPGVVGVAPTGIPAAVEGIVPSAMSLSHGLPPVAHPPHAPSPGQTVKPEADRDHASDQL hic1-ctbp1-7 1232955 n/a 1232960 unspecified role unspecified role bait bait n/a 1232961 unspecified role unspecified role prey prey physical association physical association false false false 2C hic1-ctbp2-3 1232955 n/a 1232960 unspecified role unspecified role bait bait Q14526:p.Leu244Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false n/a 1232962 unspecified role unspecified role prey prey P56546:p.Ala58Glu mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false P56546:p.Val72Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 2C , 3B and 4C hic1-ctbp1-4 1232956 n/a 1232960 unspecified role unspecified role bait bait n/a 1232963 unspecified role unspecified role prey prey physical association physical association false false false 2A and B hic1-ctbp1-9 1232957 n/a 1232961 unspecified role unspecified role prey prey n/a 1232960 unspecified role unspecified role bait bait Q14526:p.Leu244Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1D and 4B hic1-ctbp2-2 1232958 n/a 1232962 unspecified role unspecified role prey prey P56546:p.Ala58Glu mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false P56546:p.Val72Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false n/a 1232960 unspecified role unspecified role bait bait physical association physical association false false false 3A hic1-ctbp1-8 1232959 n/a 1232961 unspecified role unspecified role prey prey n/a 1232960 unspecified role unspecified role bait bait Q14526:p.Leu244Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 4A