IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ zhang-2006e-3 RNA-binding proteins SOP-2 and SOR-1 form a novel PcG-like complex in C. elegans. in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Zhang T., Sun Y., Tian E., Deng H., Zhang Y., Luo X., Cai Q., Wang H., Chai J., Zhang H. zhanghong@nibs.ac.cn Development (0950-1991) 2006 Only protein-protein interactions imex curation zhang-2006e-1 RNA-binding proteins SOP-2 and SOR-1 form a novel PcG-like complex in C. elegans. in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa predetermined predetermined participant predetermined Zhang T., Sun Y., Tian E., Deng H., Zhang Y., Luo X., Cai Q., Wang H., Chai J., Zhang H. zhanghong@nibs.ac.cn Development (0950-1991) 2006 Only protein-protein interactions imex curation zhang-2006e-2 RNA-binding proteins SOP-2 and SOR-1 form a novel PcG-like complex in C. elegans. caeel-embryo Caenorhabditis elegans embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Zhang T., Sun Y., Tian E., Deng H., Zhang Y., Luo X., Cai Q., Wang H., Chai J., Zhang H. Untagged protein identified by immunostaining zhanghong@nibs.ac.cn Development (0950-1991) 2006 Only protein-protein interactions imex curation zhang-2006e-4 RNA-binding proteins SOP-2 and SOR-1 form a novel PcG-like complex in C. elegans. ecoli Escherichia coli (strain K12) pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Zhang T., Sun Y., Tian E., Deng H., Zhang Y., Luo X., Cai Q., Wang H., Chai J., Zhang H. Proteins were purified by the GST affinity column followed by ion exchange and Superdex columns. zhanghong@nibs.ac.cn Development (0950-1991) 2006 Only protein-protein interactions imex curation sop2_caeel Polycomb protein sop-2 C50E10.4 sop-2 Suppressor of pal-1 protein 2 protein protein caeel Caenorhabditis elegans MSSNLTSNEMSSTSAIVEPPEAVKGNERDKSTRRSTSQVVRPEKHEVENALEDQCSSSSLPKEAQYYAKLDKKLEGKDPRSQFYEAVRLSADIFAHKFEKAVCSRQTFEPTNSIIKVLNTAEEEMLHEKVVPLPVSSKLQYYLNRGRYDTIFDRDEQLQRTADPMADEPDHVEKRVTSILEQASREMEEGEVEPVFYDGSDEDQELPIDLGAMRNLQRTNKFAARSSRMAARRGGRPGYRGAFRGAARGAPSRRPAPAAEVAPETPVAAPMAPAAPAAPATPEAAPAAEVMDTSIATEMPQESAVDLSNVSAATEMDTSKEGEASRPTSEKKKKIRTTEMDRLMSMDLGPKDGGRVGELGHMWPESRRRPAAPLPETPAAQPRKSLPRRAAEKKKPEDSDAAEEQEVEMEVDNDASTSTPRNARGGRGGGNRRGSRRGQKRTSGGSGKLVEPKKEPVDEPAEKIPKRSEAAPEVPATATTKEAPPSTSSSPPDAPATPATPASSDSRDSPRKIRAMIFSLTGSPPESETPPVLQQEQVISTAAPTAGRHPNIIQQVPHINRIPPQPLRRLTAPQAPPASQPEEPPVQQTVPVVKVELASAPAPIVRDPQSTEPVPPAMPTLVENNHEATLILPPNKTSDYTRWNAQDLINWVRLLITNNVDSTIAIMVREEFDGETLACLVLDDDFRKEVPIPYGHYKKMKIYGTEVLNHYRTEKYQADLRKFHEELAAWKAQQR sor1_caeel Sop-2-related protein 1 ZK1236.3 sor-1 protein protein caeel Caenorhabditis elegans MTINIKYSSKFSSSKTSSSEELKPKTYIPAYYQPPVSMPKYYVNWLRIKLSLNKLKNIRAIYLFDQCQNFNQYQESSRKFSAGQVSSLTPWYSNFSNYSTVILRMKDTSLPPLENPSNGGTYLFNLITVFPSIALSFNYSWRGDTGPSISIFSFSLSVLFFSLFPQHKNICARAWCRPFRRSLSFSLRFIMSSEPASSSTEKVPEEPHPHSIKHKFQGPQFVIPRALSDYVLNVNNQTPESYEKALNAKYGRDDYITLCLHVTSLCTEYGPLDDGPEYVLLCDSRARVDKLIEDLVKLLEIDTDYVQLELHGGKRLHLQKPDAVLRDIAYKQSNEGSDKFFLEMKLVPSESMKAKIMKQEEEEEKARKHGQYQQYQEYHQQHQAMNDGQSSSSVPSTSSPSCSSEANRKEMETVREPAGPSELMRAINAPVAPAPVVIKIETPVALPEEDETLMDDDEMPSLTVEAPSEEASFEAEQPSPQVPQASIEGPSQQQQIPGTSQQKRQVARGSRTNMISYHDLPPGTGNAPPMACPQVTLKLEKNVPFEAKIRAVAGYTRKPISEVQKMRPSDLESIFHSICIASVQRIKRRNELVQQLQEINAQSCKSPTMTMNKKFTLAKAYQRVQNEIEKIDREQILPQQYMNMPPMPPQGQQRLPPPAYPPGILPPQQNRQQGVPPQFQRSPQFMIGPDGQRYAHPYMQLPNSNQRARILNTSSVQPSEEVRNRLVKIEAMAMNMAQLNPPRPPPPQPPHRALQGELQFLRPGAPDPCNFRPDSKQTYNNTYVTVASPATLTNSIIPWHFPPYEKSGRLNVSNTIKAINEYRLLCNSRQADPASFLEFYFLGDPMPHFNKILSIADYNMYLSRRRCDEADVKIHRMSHSDQLQLYLLELQSDESNVEKWKTFYRIMQWDLPLNNEFPRILLPSSLDIGRPVVDRKKKSIDQVMNHIHRMHSQRPPSMGNSSTSSEASSTSPTNAATATSSPASNRPTTSTAQPPTLNPT egl-5_5utr_rna rna ribonucleic acid RNA rna chemical synthesis Chemical synthesis (Chemical synthesis) 5′UTR of egl-5, C08C3 nucleotides 41280-41840) sop_2-sor_1-3 1145074 n/a 1145078 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1145079 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro 1145086 1145084 direct interaction direct interaction false false false Fig 5A sor_1-sop_2 1145074 n/a 1145078 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro n/a 1145079 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) 1145093 1145090 direct interaction direct interaction false false false Fig 5B egl_5-sor_1 1145075 n/a 1145079 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1145080 unspecified role unspecified role bait bait region radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false Fig 3C sop_2-sor_1-1 1145076 n/a 1145079 unspecified role unspecified role neutral component neutral component n/a 1145078 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Fig 4E sop_2-sor_1-5 1145077 n/a 1145078 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1145079 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 1145110 1145108 physical association physical association false false false Fig 5D