UniProt UniProt http://www.uniprot.org cao-2005-2 Role of Bmi-1 and Ring1A in H2A ubiquitylation and Hox gene silencing. in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Cao R., Tsukada Y., Zhang Y. yi_zhang@med.unc.edu Mol. Cell (1097-2765) 2005 Only protein-protein interactions mimix curation cao-2005-1 Role of Bmi-1 and Ring1A in H2A ubiquitylation and Hox gene silencing. spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection anti tag coip anti tag coimmunoprecipitation anti tag coip weight by staining molecular weight estimation by staining weight by staining Cao R., Tsukada Y., Zhang Y. yi_zhang@med.unc.edu Mol. Cell (1097-2765) 2005 Only protein-protein interactions mimix curation cao-2005-3 Role of Bmi-1 and Ring1A in H2A ubiquitylation and Hox gene silencing. spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Cao R., Tsukada Y., Zhang Y. yi_zhang@med.unc.edu Mol. Cell (1097-2765) 2005 Only protein-protein interactions mimix curation ring1_mouse E3 ubiquitin-protein ligase RING1 Ring1 Ring1A Rnf1 RING finger protein 1 Polycomb complex protein RING1 Transcription repressor Ring1A RING-type E3 ubiquitin transferase RING1 protein protein mouse Mus musculus Mouse MTTPANAQNASKTWELSLYELHRTPQEAIMDGTEIAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCSDCIVTALRSGNKECPTCRKKLVSKRSLRPDPNFDALISKIYPSREEYEAHQDRVLIRLSRLHNQQALSSSIEEGLRMQAMHRAQRVRRPMPGSDQTATMSGGEGEPGEGEGDGEDVSSDSAPDSAPGPAPKRPRGAGAGASSVGTGGGAAGGACGGAGSEDSGDRGGTLGGGTLGPPSPPGAPSPPEPGGEIELVFRPHPLLVEKGEYCQTRYVKTTGNATVDHLSKYLALRIALERRQQQETTEPGGPGGGASDTGGPDGGGGERGVAGGGEGPEEPALPSLEGVSEKQYTIYIAPGGGAFTTLNGSLTLELVNEKFWKVSRPLELCYAPTKDPK bmi1_mouse Polycomb complex protein BMI-1 Bmi1 Pcgf4 Bmi-1 Polycomb group RING finger protein 4 protein protein mouse Mus musculus Mouse MHRTTRIKITELNPHLMCVLCGGYFIDATTIIECLHSFCKTCIVRYLETSKYCPICDVQVHKTRPLLNIRSDKTLQDIVYKLVPGLFKNEMKRRRDFYAAHPSADAANGSNEDRGEVADEEKRIITDDEIISLSIEFFDQSRLDRKVNKEKPKEEVNDKRYLRCPAAMTVMHLRKFLRSKMDIPNTFQIDVMYEEEPLKDYYTLMDIAYIYTWRRNGPLPLKYRVRPTCKRMKMSHQRDGLTNAGELESDSGSDKANSPAGGVPSTSSCLPSPSTPVQSPHPQFPHISSTMNGTSNSPSANHQSSFASRPRKSSLNGSSATSSG cbx8_mouse Chromobox protein homolog 8 Cbx8 Pc3 Polycomb 3 homolog protein protein mouse Mus musculus Mouse MELSAVGERVFAAEALLKRRIRKGRMEYLVKWKGWSQKYSTWEPEENILDARLLAAFEEREREMELYGPKKRGPKPKTFLLKAQAKAKAKTYEFRSDSTRGIRIPYPGRSPQDLASTSRAREGLRNTGLPPPGSSTSTCRADPPRDRDRERDRGTSRVDDKPSSPGDSSKKRGPKPRKEPLDPSQRPLGEPSAGLGEYLKGRKLDETSSGTGKFPAGHSVIQLARRQDSDLVQYGVTSPSSAEASSKLAVDTFPARVIKHRAAFLEAKGQGALDPGGARVRHSSGTPASVGSLYRDMGAQGGRPSLIARIPVARILGDPEEESWSPSLTNLEKVVVTDVTSNFLTVTIKESNTDQGFFKEKR phc2_mouse Polyhomeotic-like protein 2 p36 Early development regulatory protein 2 Phc2 Edr2 Ph2 protein protein mouse Mus musculus Mouse MENELPVPHTSNRASVTTNTSGTNSSSGCISSSGGGGGSGGRPTAPQISVYSGIPDRQTVQVIQQALHRQPSTAAQYLQQMYAAQQQHLMLQTAALQQQHLSSAQLQSLAAVQQASLVANRQGSTPGSSVSSQAPAQSSSLNLAASPAAAQLINRAQSVNSAAASGLAQQAVLLGNTSSPALTASQAQMYLRAQMLIFTPTATVATVQPELCTGSPARPPTPAQVQNLTLRTQQTPAAAASGPPPTQPVLPSLALKPTPSSSQPLPAPPQGRTMAQGSPAGAKPSGTDNAPETLKAGDGNCNMEGRPGPGRAVPAVATHPLIAPAYAHLQSHQLLPQPPAKHPQPQFVAQQQPQPPRPAPQVQSQPQLASVSPSLALQSSPEDHALPLGSVTQALPLQCSTTHVHKPGNSQQCHLPTLDTGSQNGHPEGGSHPPQRRFQHTSAVILQVQPASPVTPQQCAPDDWKEVVPAEKSVPVARPGPSPHQQAIIPAIPGGLPGPKSPNIQQCPAHETGQGIVHALTDLSSPGMTSGNGNSASSIAGTAPQNGENKPPQAIVKPQILTHVIEGFVIQEGAEPFPVGRSSLLVGNLKKKYAQGFLPEKPPQQDHTTTTDSEMEEPYLQESKEEGTPLKLKCELCGRVDFAYKFKRSKRFCSMACAKRYNVGCTKRVGLFHSDRSKLQKAGTTTHNRRRASKASLPTLTKDTKKQPSGTVPLSVTAALQLAHSQEDSSRCSDNSSYEEPLSPISASSSTSRRRQGQRDLDLPDMHMRDLVGVGHHFLPSEPTKWNVEDVYEFIRSLPGCQEIAEEFRAQEIDGQALLLLKEDHLMSAMNIKLGPALKIYARISMLKDS ring2_mouse E3 ubiquitin-protein ligase RING2 Rnf2 DinG Ring1b RING finger protein 2 RING finger protein 1B RING-type E3 ubiquitin transferase RING2 protein protein mouse Mus musculus Mouse MSQAVQTNGTQPLSKTWELSLYELQRTPQEAITDGLEIVVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCADCIITALRSGNKECPTCRKKLVSKRSLRPDPNFDALISKIYPSRDEYEAHQERVLARINKHNNQQALSHSIEEGLKIQAMNRLQRGKKQQIENGSGAEDNGDSSHCSNASTHSNQEAGPSNKRTKTSDDSGLELDNNNAAVAIDPVMDGASEIELVFRPHPTLMEKDDSAQTRYIKTSGNATVDHLSKYLAVRLALEELRSKGESNQMNLDTASEKQYTIYIATASGQFTVLNGSFSLELVSEKYWKVNKPMELYYAPTKEHK pcgf2_mouse Polycomb group RING finger protein 2 Pcgf2 Mel18 Mel-18 Rnf110 Zfp144 Znf144 DNA-binding protein Mel-18 Melanoma nuclear protein 18 RING finger protein 110 Zinc finger protein 144 protein protein mouse Mus musculus Mouse MHRTTRIKITELNPHLMCALCGGYFIDATTIVECLHSFCKTCIVRYLETNKYCPMCDVQVHKTRPLLSIRSDKTLQDIVYKLVPGLFKDEMKRRRDFYAAYPLTEVPNGSNEDRGEVLEQEKGALGDDEIVSLSIEFYEGVRDREEKKNLTENGDGDKEKTGVRFLRCPAAMTVMHLAKFLRNKMDVPSKYKVEILYEDEPLKEYYTLMDIAYIYPWRRNGPLPLKYRVQPACKRLTLPTVPTPSEGTNTSGASECESVSDKAPSPATLPATSSSLPSPATPSHGSPSSHGPPATHPTSPTPPSTAAGTTTATNGGTSNCLQTPSSTSRGRKMTVNGAPCPP ring1-bmi1-1 1157963 n/a 1157966 unspecified role unspecified role bait bait n/a 1157967 unspecified role unspecified role prey prey physical association physical association false false false 2a ring1-cbx8 1157963 n/a 1157966 unspecified role unspecified role bait bait n/a 1157968 unspecified role unspecified role prey prey physical association physical association false false false 2a ring1-phc2 1157963 n/a 1157969 unspecified role unspecified role prey prey n/a 1157966 unspecified role unspecified role bait bait physical association physical association false false false 2a rnf2-bmi1 1157963 n/a 1157967 unspecified role unspecified role prey prey n/a 1157970 unspecified role unspecified role bait bait physical association physical association false false false 2a rnf2-cbx8 1157963 n/a 1157970 unspecified role unspecified role bait bait n/a 1157968 unspecified role unspecified role prey prey physical association physical association false false false 2a rnf2-phc2 1157963 n/a 1157969 unspecified role unspecified role prey prey n/a 1157970 unspecified role unspecified role bait bait physical association physical association false false false 2a bmi1-ring1-1 1157963 n/a 1157967 unspecified role unspecified role bait bait n/a 1157966 unspecified role unspecified role prey prey physical association physical association false false false 2a bmi1-rnf2 1157963 n/a 1157967 unspecified role unspecified role bait bait n/a 1157970 unspecified role unspecified role prey prey physical association physical association false false false 2a bmi1-bmi1-3 1157963 n/a 1157967 unspecified role unspecified role bait bait n/a 1157967 unspecified role unspecified role prey prey physical association physical association false false false 2a bmi1-cbx8-1 1157963 n/a 1157968 unspecified role unspecified role prey prey n/a 1157967 unspecified role unspecified role bait bait physical association physical association false false false 2a bmi1-phc2 1157963 n/a 1157969 unspecified role unspecified role prey prey n/a 1157967 unspecified role unspecified role bait bait physical association physical association false false false 2a cbx8-ring1-3 1157963 n/a 1157968 unspecified role unspecified role bait bait n/a 1157966 unspecified role unspecified role prey prey physical association physical association false false false 2a cbx8-rnf2-2 1157963 n/a 1157970 unspecified role unspecified role prey prey n/a 1157968 unspecified role unspecified role bait bait physical association physical association false false false 2a cbx8-bmi1-2 1157963 n/a 1157968 unspecified role unspecified role bait bait n/a 1157967 unspecified role unspecified role prey prey physical association physical association false false false 2a phc2-ring1 1157963 n/a 1157969 unspecified role unspecified role bait bait n/a 1157966 unspecified role unspecified role prey prey physical association physical association false false false 2a phc2-rnf2 1157963 n/a 1157969 unspecified role unspecified role bait bait n/a 1157970 unspecified role unspecified role prey prey physical association physical association false false false 2a phc2-bmi1 1157963 n/a 1157969 unspecified role unspecified role bait bait n/a 1157967 unspecified role unspecified role prey prey physical association physical association false false false 2a ring1-rnf2-3 1157963 n/a 1157966 unspecified role unspecified role bait bait n/a 1157970 unspecified role unspecified role prey prey physical association physical association false false false 2a ring1-ring1 1157963 n/a 1157966 unspecified role unspecified role bait bait n/a 1157966 unspecified role unspecified role prey prey physical association physical association false false false 2a bmi1-cbx8-4 1157964 n/a 1157967 unspecified role unspecified role bait bait n/a 1157970 unspecified role unspecified role prey prey n/a 1157966 unspecified role unspecified role prey prey n/a 1157968 unspecified role unspecified role prey prey association association false false false 1b pcgf2-cbx8-2 1157964 n/a 1157966 unspecified role unspecified role prey prey n/a 1157971 unspecified role unspecified role bait bait n/a 1157970 unspecified role unspecified role prey prey n/a 1157968 unspecified role unspecified role prey prey association association false false false 6b bmi1-ring1-2 1157965 n/a 1157970 unspecified role unspecified role neutral component neutral component n/a 1157966 unspecified role unspecified role neutral component neutral component n/a 1157967 unspecified role unspecified role neutral component neutral component physical association physical association false false false 6a