IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ crowe-2004-1 Characterization of C. elegans RING finger protein 1, a binding partner of ubiquitin-conjugating enzyme 1. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Crowe E., Candido EP. croweemily2002@yahoo.com, epmc@interchange.ubc.ca a random primed C. elegans cDNA library. Dev. Biol. (0012-1606) 2004 Only protein-protein interactions imex curation crowe-2004-2 Characterization of C. elegans RING finger protein 1, a binding partner of ubiquitin-conjugating enzyme 1. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Crowe E., Candido EP. croweemily2002@yahoo.com, epmc@interchange.ubc.ca a random primed C. elegans cDNA library. Dev. Biol. (0012-1606) 2004 Only protein-protein interactions imex curation crowe-2004-3 Characterization of C. elegans RING finger protein 1, a binding partner of ubiquitin-conjugating enzyme 1. caeel-non_gavid_adul Caenorhabditis elegans non-gavid adult non_gravid_adult Adult anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Crowe E., Candido EP. croweemily2002@yahoo.com, epmc@interchange.ubc.ca a random primed C. elegans cDNA library. Dev. Biol. (0012-1606) 2004 Only protein-protein interactions imex curation ubc1_caeel Ubiquitin-conjugating enzyme E2 1 E2 ubiquitin-conjugating enzyme 1 ubc-1 C35B1.1 Ubiquitin-protein ligase 1 Ubiquitin carrier protein 1 protein protein caeel Caenorhabditis elegans MTTPSRRRLMRDFKKLQEDPPAGVSGAPTEDNILTWEAIIFGPQETPFEDGTFKLSLEFTEEYPNKPPTVKFISKMFHPNVYADGSICLDILQNRWSPTYDVAAILTSIQSLLDEPNPNSPANSLAAQLYQENRREYEKRVQQIVEQSWLNFGENEGDAVLKDDVEIEEIAAPGANDADDDRMDEGASGSNA ubr1_caeel E3 ubiquitin-protein ligase ubr-1 ubr-1 N-recognin-1 Ubiquitin-protein ligase E3-alpha C32E8.11 RING-type E3 ubiquitin transferase ubr-1 protein protein caeel Caenorhabditis elegans MIVDLIQSARQGEWAQVRQLLLKHWLVQVPEVFEVNSDLPWDNTAANERILGSQGEILLAPLVSAFVLDVRNTKSTLEAMNGIAGVDPARRGQICGHVFKNGELTYTCLDCATDGTCVMCLQCFEVSIHKSHKYKMHSSSGSGYCDCGDADAWTEGYACANHEKKDDEEAAVLAPELKKRCEQLVEIILQFSLSMITHKDDLKLPEIFEKMKPEVTNEAQQYLTVLYNDETHTYESVIKVLELYIHCTKDQAMLVATIVDREGRSAVKLGSKADCTKAKDDVQRKTARDPTSIRRSSNHNLPLSVKVMDTTLFALQNFSISLLTWLNTQMDVFPPLREIVGEILLSSKFALKKNYTRKMKSEDQRLVAGIIRNVMVLPDDEEEELFALDGRMDVDEMDDDDIGGEALQMEIDADDEEEITAALAGVSEHQESPGPSRDSSTFTMLENILLQDTQMWKAGRSILHQMLMRTVFMIYDQKVRFAKAFMLHYNEIYEDFIKDDHEMDVSVVGLSVQFMTVPSLARKLVAEDQAFSVISKAIRDQTDKFVKYYNDGKIARFDFTSRSFPPELRRSLHITRDMAYILNAVPSESDWNRELIDGFVQGFADFLLFLQHLQGMDEVKRQAVEHQVWESEWETAFNILLRLKDAISMIIGWAETNEEVHNRLMIMCLELMNRMPPVYTKSEEDTYELTVTINGESCRISHFDVLKSSTSVHQPVVRIIAGLFSASNYTGFLLNRSNNSHTSLNQERIKELINCKDETNLYELSLRVLVLCAQSNATLWRRNGFSLINQIHNYFSPLCRNEMFDRDVLMMQVGAALTPSTKFIFHLLHRFRLFKWATSEFEQDKANEKPAKPESEDLSKTLVMIAEEFIQCLILILCERYTYGVGKTRPMDQMKREVIHILCTGSHTFSHIQQKMSHDINSKRLSLHEAVNLVADFRKPLATTAGQFHCKESSLPTYSPFFMHYSKSDQSAAEQSQARVRAKMEKSVRACAPPILPDFQTFFERIPEILTTNVLIHVVRLIIDRTARRSRFSSDRLFHKTLYLIGIALNEEEKNPSFGFTQRAEESIGLLSLLEGLVGKPESSICPILLEVTVEKYRKLIKARAGVPEAAPAPENKPAQSEEEIKAKRAARAAEMRQKAMAKMSNMQSKFMKKIEDEEKKDESQTPSEKSETVVKKDDYDNKHFFDEDVVKQVGHDFPVCIGANKWHAELVKPRTLTCILCQEDEIIAPQQGKPMVCAAFIQQSQLFTHKNKNGELMTASSGTISTRDLLTAPATLQYGVDVSTCSHSMHYECYRSLAEANRSRESLRARQVGQHSHKMVDTENGEYQCPLCKRLSNAAIPVLPAYQLTNQNGFSTVSGAGKENFDTWVARVKRNLEMPLSSESVSKKGHSRKRSHSERSLLDLEKLSKDPDTANTSAGVLQFGAMEMSSATHMPASAESQMLMTTSPSQDDVEFYNELAAMFVDQDVNNTTSPAATPETIPAIGSSSRIPESQESGKKPLSSQIQHVLYSLIRPFPALINSNRICSSSFEGFEEPIKDLGKNMMKFRKRGNELKTNFIEKHLKGYVISTVTWQSTAHVARAISSYLHYDKKPLFGALNTRQRDCLSAMARLCASLSHNMQFLLHAVSDMLRVFLCEPPRPKLAQTPGSPLLSAPSTSSFTPAPAQIPHSGTNFAFLVQLFNPAGPRKNVNLNILQIDILSLAISLMMTIGWTWNNGTQSMNSSSTHQKARLLTPDGSVDEAYVLRLSLLAHYFQIIATHSEADGNDVNMEEEQESQMEVDPVAAQTIRKLYALCHPFDGPLRRVDILWRKMEEGAQSLLRPIALLYHFITLVDPPEALKDPSINSSEPLFRYLGLPHKIEEQISGSMLEKLFTMWSSSIPSDQALRQDLVVQPVRPNLLVELPEKYSQLINQVATFKCPTIPIEESTSNVPTLCLVCGTILCSQAYCCQKIINKQSYGACRYHMSQCSGSVGMFLRVRDCSLVLMTTRKRGCFRPAPYVDEFGEVDQGFRRGNPLHLNPELYQKLKSLWLQQGITEEVVNYNEIDFRNVQYDWGHF bre1_caeel E3 ubiquitin-protein ligase bre-1 RING-type E3 ubiquitin transferase bre-1 rfp-1 RING finger protein rfp-1 R05D3.4 bre-1 protein protein caeel Caenorhabditis elegans MMKRSNEGIGGENYASSPSDDGQQKRRKIQFEPVRMPAVSNVNDIRARAVVYQTSKLKQQLLYKNKRIAELEKENERSKRRQQTDESNFLKVYNMFSDIEKYICTQTKNEFGEYIGGDTAPTGIDVLGMTNETYNKFFDQAKQNLRNAFVSYAKARHDRAHESTIFIDKLKTLIDSPTFNPNGVHKELTAKAASLAIQNEKLQSEVTKVQSDCYNLERKKRILTDKLSVQENRVQELEHQLEDARFETDKHMRLANKFEYKLATLVSEGQSGGNGGATPSSSGTTNATEKKISAPDIPPSETAAKEIENLRLERDEQESIASRRLQDLEEMNKKVQTLTQENSKLRLETQTFFSVDSIVNSEEYKNLKKYYSLAIKEYERVSKDLEDITTERDAFRSAKEARAMLMSEEHQKTLKEIQCQSDIHNSFYKVSHDSEVLRCEFETVKEEYNKTVKQSEWDEMKATLNTLRSMNRSLKSEKIRLREKDKQSQKDINTLKSELTSLKEAQDKCLLVPLEDVSNAPPEDVNKIRQEYESLCKEVKRLGAMEKQEKQKQVENLQKEVNRQIADKLSELETLRKTNEMLTNDEECISDELEAIGTAVEEEQERNAQLYIEKREQEDRNLKMMNDRMIQNQTFNRLREKLSCLESKAQTDAQIAKMHEFEKKANEELVTKLSESVQFKSAELTRLTNLMEQHRKNIQEVGMSRDENQIKADRCEGQMKQIQELYAAKAREIEDFKFKRQRAEEELETLRIKYERVKRNESVPAQSGDQVLEEANRQMKETLTCPSCKTRPKDCIMLKCYHLFCETCIKTMYDTRQRKCPKCNSNFGANDFHRIFI ubc_1-rfp_1-3 1136504 n/a 1136507 unspecified role unspecified role bait bait n/a 1136508 unspecified role unspecified role prey prey physical association physical association false false false Results Paragraph 1 ubc_1-bre 1136504 n/a 1136509 unspecified role unspecified role prey prey n/a 1136507 unspecified role unspecified role bait bait physical association physical association false false false Results Paragraph 1 rfp_1-ubc_1-1 1136505 n/a 1136509 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1136507 unspecified role unspecified role prey prey physical association physical association false false false Fig 2 rfp_1-ubc_1 1136506 n/a 1136509 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1136507 unspecified role unspecified role prey prey physical association physical association false false false Fig 3