UniProt UniProt http://www.uniprot.org raijmakers-2003-1 The association of the human PM/Scl-75 autoantigen with the exosome is dependent on a newly identified N terminus. human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Raijmakers R., Egberts WV., van Venrooij WJ., Pruijn GJ. For Participant Detection a human serum (Ven96) was used, which is reactive with several exosome proteins (including PM/Scl-100 (EXOSC10), PM/Scl-75 (EXOSC9), hRr4p (EXOSC2), hRrp41p (EXOSC4), and hRrp42p (EXOSC7). The proteins have been mapped through their specific molecular weights). g.prujin@ncmls.kun.nl J. Biol. Chem. (0021-9258) 2003 mimix curation raijmakers-2003-2 The association of the human PM/Scl-75 autoantigen with the exosome is dependent on a newly identified N terminus. human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Raijmakers R., Egberts WV., van Venrooij WJ., Pruijn GJ. For Participant Detection a human serum (Ven96) was used, which is reactive with several exosome proteins (including PM/Scl-100 (EXOSC10), PM/Scl-75 (EXOSC9), hRr4p (EXOSC2), hRrp41p (EXOSC4), and hRrp42p (EXOSC7). The proteins have been mapped through their specific molecular weights). g.prujin@ncmls.kun.nl J. Biol. Chem. (0021-9258) 2003 mimix curation raijmakers-2003-3 The association of the human PM/Scl-75 autoantigen with the exosome is dependent on a newly identified N terminus. chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Raijmakers R., Egberts WV., van Venrooij WJ., Pruijn GJ. For Participant Detection a human serum (Ven96) was used, which is reactive with several exosome proteins (including PM/Scl-100 (EXOSC10), PM/Scl-75 (EXOSC9), hRr4p (EXOSC2), hRrp41p (EXOSC4), and hRrp42p (EXOSC7). The proteins have been mapped through their specific molecular weights). g.prujin@ncmls.kun.nl J. Biol. Chem. (0021-9258) 2003 mimix curation q06265-1 Exosome complex component RRP45 PM/SCL-75c-alpha EXOSC9 PMSCL1 Autoantigen PM/Scl 1 Exosome component 9 P75 polymyositis-scleroderma overlap syndrome-associated autoantigen Polymyositis/scleroderma autoantigen 1 Polymyositis/scleroderma autoantigen 75 kDa protein protein human Homo sapiens Human MKETPLSNCERRFLLRAIEEKKRLDGRQTYDYRNIRISFGTDYGCCIVELGKTRVLGQVSCELVSPKLNRATEGILFFNLELSQMAAPAFEPGRQSDLLVKLNRLMERCLRNSKCIDTESLCVVAGEKVWQIRVDLHLLNHDGNIIDAASIAAIVALCHFRRPDVSVQGDEVTLYTPEERDPVPLSIHHMPICVSFAFFQQGTYLLVDPNEREERVMDGLLVIAMNKHREICTIQSSGGIMLLKDQVLRCSKIAGVKVAEITELILKALENDQKVRKEGGKFGFAESIANQRITAFKMEKAPIDTSDVEEKAEEIIAEAEPPSEVVSTPVLWTPGTAQIGEGVENSWGDLEDSEKEDDEGGGDQAIILDGIKMDTGVEVSDIGSQDAPIILSDSEEEEMIILEPDKNPKKIRTQTTSAKQEKAPSKKPVKRRKKKRAAN exosx_human Exosome component 10 PMSCL2 EXOSC10 PMSCL Polymyositis/scleroderma autoantigen 2 Autoantigen PM/Scl 2 Polymyositis/scleroderma autoantigen 100 kDa P100 polymyositis-scleroderma overlap syndrome-associated autoantigen RRP6 protein protein human Homo sapiens Human MAPPSTREPRVLSATSATKSDGEMVLPGFPDADSFVKFALGSVVAVTKASGGLPQFGDEYDFYRSFPGFQAFCETQGDRLLQCMSRVMQYHGCRSNIKDRSKVTELEDKFDLLVDANDVILERVGILLDEASGVNKNQQPVLPAGLQVPKTVVSSWNRKAAEYGKKAKSETFRLLHAKNIIRPQLKFREKIDNSNTPFLPKIFIKPNAQKPLPQALSKERRERPQDRPEDLDVPPALADFIHQQRTQQVEQDMFAHPYQYELNHFTPADAVLQKPQPQLYRPIEETPCHFISSLDELVELNEKLLNCQEFAVDLEHHSYRSFLGLTCLMQISTRTEDFIIDTLELRSDMYILNESLTDPAIVKVFHGADSDIEWLQKDFGLYVVNMFDTHQAARLLNLGRHSLDHLLKLYCNVDSNKQYQLADWRIRPLPEEMLSYARDDTHYLLYIYDKMRLEMWERGNGQPVQLQVVWQRSRDICLKKFIKPIFTDESYLELYRKQKKHLNTQQLTAFQLLFAWRDKTARREDESYGYVLPNHMMLKIAEELPKEPQGIIACCNPVPPLVRQQINEMHLLIQQAREMPLLKSEVAAGVKKSGPLPSAERLENVLFGPHDCSHAPPDGYPIIPTSGSVPVQKQASLFPDEKEDNLLGTTCLIATAVITLFNEPSAEDSKKGPLTVAQKKAQNIMESFENPFRMFLPSLGHRAPVSQAAKFDPSTKIYEISNRWKLAQVQVQKDSKEAVKKKAAEQTAAREQAKEACKAAAEQAISVRQQVVLENAAKKRERATSDPRTTEQKQEKKRLKISKKPKDPEPPEKEFTPYDYSQSDFKAFAGNSKSKVSSQFDPNKQTPSGKKCIAAKKIKQSVGNKSMSFPTGKSDRGFRYNWPQR exos4_human Exosome complex component RRP41 EXOSC4 RRP41 SKI6 Ribosomal RNA-processing protein 41 Exosome component 4 p12A protein protein human Homo sapiens Human MAGLELLSDQGYRVDGRRAGELRKIQARMGVFAQADGSAYIEQGNTKALAVVYGPHEIRGSRARALPDRALVNCQYSSATFSTGERKRRPHGDRKSCEMGLQLRQTFEAAILTQLHPRSQIDIYVQVLQADGGTYAACVNAATLAVLDAGIPMRDFVCACSAGFVDGTALADLSHVEEAAGGPQLALALLPASGQIALLEMDARLHEDHLERVLEAAAQAARDVHTLLDRVVRQHVREASILLGD exos2_human Exosome complex component RRP4 EXOSC2 RRP4 Ribosomal RNA-processing protein 4 Exosome component 2 protein protein human Homo sapiens Human MAMEMRLPVARKPLSERLGRDTKKHLVVPGDTITTDTGFMRGHGTYMGEEKLIASVAGSVERVNKLICVKALKTRYIGEVGDIVVGRITEVQQKRWKVETNSRLDSVLLLSSMNLPGGELRRRSAEDELAMRGFLQEGDLISAEVQAVFSDGAVSLHTRSLKYGKLGQGVLVQVSPSLVKRQKTHFHDLPCGASVILGNNGFIWIYPTPEHKEEEAGGFIANLEPVSLADREVISRLRNCIISLVTQRMMLYDTSILYCYEASLPHQIKDILKPEIMEEIVMETRQRLLEQEG exos7_human Exosome complex component RRP42 KIAA0116 RRP42 EXOSC7 Ribosomal RNA-processing protein 42 Exosome component 7 p8 protein protein human Homo sapiens Human MASVTLSEAEKVYIVHGVQEDLRVDGRGCEDYRCVEVETDVVSNTSGSARVKLGHTDILVGVKAEMGTPKLEKPNEGYLEFFVDCSASATPEFEGRGGDDLGTEIANTLYRIFNNKSSVDLKTLCISPREHCWVLYVDVLLLECGGNLFDAISIAVKAALFNTRIPRVRVLEDEEGSKDIELSDDPYDCIRLSVENVPCIVTLCKIGYRHVVDATLQEEACSLASLLVSVTSKGVVTCMRKVGKGSLDPESIFEMMETGKRVGKVLHASLQSVVHKEESLGPKRQKVGFLG exos1_human Exosome complex component CSL4 CSL4 EXOSC1 CGI-108 Exosome component 1 protein protein human Homo sapiens Human MAPPVRYCIPGERLCNLEEGSPGSGTYTRHGYIFSSLAGCLMKSSENGALPVVSVVRETESQLLPDVGAIVTCKVSSINSRFAKVHILYVGSMPLKNSFRGTIRKEDVRATEKDKVEIYKSFRPGDIVLAKVISLGDAQSNYLLTTAENELGVVVAHSESGIQMVPISWCEMQCPKTHTKEFRKVARVQPEFLQT q06265-3 Exosome complex component RRP45 PM/SCL-75a-alpha EXOSC9 PMSCL1 Autoantigen PM/Scl 1 Exosome component 9 P75 polymyositis-scleroderma overlap syndrome-associated autoantigen Polymyositis/scleroderma autoantigen 1 Polymyositis/scleroderma autoantigen 75 kDa protein protein human Homo sapiens Human MAAPAFEPGRQSDLLVKLNRLMERCLRNSKCIDTESLCVVAGEKVWQIRVDLHLLNHDGNIIDAASIAAIVALCHFRRPDVSVQGDEVTLYTPEERDPVPLSIHHMPICVSFAFFQQGTYLLVDPNEREERVMDGLLVIAMNKHREICTIQSSGGIMLLKDQVLRCSKIAGVKVAEITELILKALENDQKVRKEGGKFGFAESIANQRITAFKMEKAPIDTSDVEEKAEEIIAEAEPPSEVVSTPVLWTPGTAQIGEGVENSWGDLEDSEKEDDEGGGDQAIILDGIKMDTGVEVSDIGSQDAPIILSDSEEEEMIILEPDKNPKKIRTQTTSAKQEKAPSKKPVKRRKKKRAAN q06265-2 Exosome complex component RRP45 PM/SCL-75c-beta EXOSC9 PMSCL1 Autoantigen PM/Scl 1 Exosome component 9 P75 polymyositis-scleroderma overlap syndrome-associated autoantigen Polymyositis/scleroderma autoantigen 1 Polymyositis/scleroderma autoantigen 75 kDa protein protein human Homo sapiens Human MKETPLSNCERRFLLRAIEEKKRLDGRQTYDYRNIRISFGTDYGCCIVELGKTRVLGQVSCELVSPKLNRATEGILFFNLELSQMAAPAFEPGRQSDLLVKLNRLMERCLRNSKCIDTESLCVVAGEKVWQIRVDLHLLNHDGNIIDAASIAAIVALCHFRRPDVSVQGDEVTLYTPEERDPVPLSIHHMPICVSFAFFQQGTYLLVDPNEREERVMDGLLVIAMNKHREICTIQSSGGIMLLKDQVLRCSKIAGVKVAEITELILKALENDQKVRKEGGKFGFAESIANQRITAFKMEKAPIDTSDVEEKAEEIIAEAEPPSEVVSTPVLWTPGTAQIGEGVENSWGDLEDSEKEDDEGGGDQAIILDGIKMDTGVEVSDIGSQELGFHHVGQTGLEFLTSDAPIILSDSEEEEMIILEPDKNPKKIRTQTTSAKQEKAPSKKPVKRRKKKRAAN Contains a phosphoserine at position 409. Contains a phosphoserine at position 411. exos9_human Exosome complex component RRP45 EXOSC9 PMSCL1 Exosome component 9 Polymyositis/scleroderma autoantigen 1 Autoantigen PM/Scl 1 Polymyositis/scleroderma autoantigen 75 kDa P75 polymyositis-scleroderma overlap syndrome-associated autoantigen protein protein human Homo sapiens Human MKETPLSNCERRFLLRAIEEKKRLDGRQTYDYRNIRISFGTDYGCCIVELGKTRVLGQVSCELVSPKLNRATEGILFFNLELSQMAAPAFEPGRQSDLLVKLNRLMERCLRNSKCIDTESLCVVAGEKVWQIRVDLHLLNHDGNIIDAASIAAIVALCHFRRPDVSVQGDEVTLYTPEERDPVPLSIHHMPICVSFAFFQQGTYLLVDPNEREERVMDGLLVIAMNKHREICTIQSSGGIMLLKDQVLRCSKIAGVKVAEITELILKALENDQKVRKEGGKFGFAESIANQRITAFKMEKAPIDTSDVEEKAEEIIAEAEPPSEVVSTPVLWTPGTAQIGEGVENSWGDLEDSEKEDDEGGGDQAIILDGIKMDTGVEVSDIGSQDAPIILSDSEEEEMIILEPDKNPKKIRTQTTSAKQEKAPSKKPVKRRKKKRAAN exosc9-exosc10-2 1130699 n/a 1130702 unspecified role unspecified role bait bait n/a 1130703 unspecified role unspecified role prey prey n/a 1130704 unspecified role unspecified role prey prey n/a 1130705 unspecified role unspecified role prey prey n/a 1130706 unspecified role unspecified role prey prey association association false false false 4A (lane 5) and 4B (lane2) exosc1-exosc2 1130699 n/a 1130705 unspecified role unspecified role prey prey n/a 1130706 unspecified role unspecified role prey prey n/a 1130707 unspecified role unspecified role bait bait n/a 1130704 unspecified role unspecified role prey prey association association false false false 4a q06265_3-exosc10 1130699 n/a 1130708 unspecified role unspecified role bait bait n/a 1130703 unspecified role unspecified role prey prey n/a 1130705 unspecified role unspecified role prey prey n/a 1130704 unspecified role unspecified role prey prey n/a 1130706 unspecified role unspecified role prey prey association association false false false 4a (lane3) exosc9-exosc10-5 1130699 n/a 1130709 unspecified role unspecified role bait bait n/a 1130703 unspecified role unspecified role prey prey n/a 1130705 unspecified role unspecified role prey prey n/a 1130704 unspecified role unspecified role prey prey n/a 1130706 unspecified role unspecified role prey prey association association false false false 4B (lane3) exosc9-exosc10-3 1130700 n/a 1130710 unspecified role unspecified role bait bait n/a 1130706 unspecified role unspecified role prey prey n/a 1130704 unspecified role unspecified role prey prey n/a 1130705 unspecified role unspecified role prey prey n/a 1130703 unspecified role unspecified role prey prey association association false false false 4A (lane 7) exosc9-exosc4 1130701 n/a 1130702 unspecified role unspecified role bait bait n/a 1130704 unspecified role unspecified role prey prey physical association physical association false false false 5