UniProt UniProt http://www.uniprot.org deltour-2002-1 The human candidate tumor suppressor gene HIC1 recruits CtBP through a degenerate GLDLSKK motif. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Deltour S., Pinte S., Guerardel C., Wasylyk B., Leprince D. For human HIC1 an isoform-specific mapping to UniProt is not possible according to the information for the genomic clone used and provided in the paper, though aa positions mentioned refer to isoform 2 (AC Q14526-2) throughout. Dominique.Leprince@ibl.fr Dominique.Leprince@ ibl.fr,Dominique.Leprince@ibl.fr Dominique.Leprince@ ibl.fr Mol. Cell. Biol. (0270-7306) 2002 Only protein-protein interactions imex curation deltour-2002-2 The human candidate tumor suppressor gene HIC1 recruits CtBP through a degenerate GLDLSKK motif. rabit-rk13 rk13 cell line originating from rabbit kidney epithelial cells rk13 RK13 cell line produced from rabbit kidney epithelial cells 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Deltour S., Pinte S., Guerardel C., Wasylyk B., Leprince D. For human HIC1 an isoform-specific mapping to UniProt is not possible according to the information for the genomic clone used and provided in the paper, though aa positions mentioned refer to isoform 2 (AC Q14526-2) throughout. Dominique.Leprince@ibl.fr Dominique.Leprince@ ibl.fr,Dominique.Leprince@ibl.fr Dominique.Leprince@ ibl.fr Mol. Cell. Biol. (0270-7306) 2002 Only protein-protein interactions imex curation deltour-2002-3 The human candidate tumor suppressor gene HIC1 recruits CtBP through a degenerate GLDLSKK motif. chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 pull down pull down anti tag western anti tag western blot anti tag western Deltour S., Pinte S., Guerardel C., Wasylyk B., Leprince D. For human HIC1 an isoform-specific mapping to UniProt is not possible according to the information for the genomic clone used and provided in the paper, though aa positions mentioned refer to isoform 2 (AC Q14526-2) throughout. Dominique.Leprince@ibl.fr Dominique.Leprince@ ibl.fr,Dominique.Leprince@ibl.fr Dominique.Leprince@ ibl.fr Mol. Cell. Biol. (0270-7306) 2002 Only protein-protein interactions imex curation deltour-2002-4 The human candidate tumor suppressor gene HIC1 recruits CtBP through a degenerate GLDLSKK motif. chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Deltour S., Pinte S., Guerardel C., Wasylyk B., Leprince D. For human HIC1 an isoform-specific mapping to UniProt is not possible according to the information for the genomic clone used and provided in the paper, though aa positions mentioned refer to isoform 2 (AC Q14526-2) throughout. Dominique.Leprince@ibl.fr Dominique.Leprince@ ibl.fr,Dominique.Leprince@ibl.fr Dominique.Leprince@ ibl.fr Mol. Cell. Biol. (0270-7306) 2002 Only protein-protein interactions imex curation deltour-2002-5 The human candidate tumor suppressor gene HIC1 recruits CtBP through a degenerate GLDLSKK motif. chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Deltour S., Pinte S., Guerardel C., Wasylyk B., Leprince D. For human HIC1 an isoform-specific mapping to UniProt is not possible according to the information for the genomic clone used and provided in the paper, though aa positions mentioned refer to isoform 2 (AC Q14526-2) throughout. Dominique.Leprince@ibl.fr Dominique.Leprince@ ibl.fr,Dominique.Leprince@ibl.fr Dominique.Leprince@ ibl.fr Mol. Cell. Biol. (0270-7306) 2002 Only protein-protein interactions imex curation deltour-2002-6 The human candidate tumor suppressor gene HIC1 recruits CtBP through a degenerate GLDLSKK motif. crigr-ecr-cho Cricetulus griseus EcR-CHO cell line ecr-cho EcR-CHO cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Deltour S., Pinte S., Guerardel C., Wasylyk B., Leprince D. For human HIC1 an isoform-specific mapping to UniProt is not possible according to the information for the genomic clone used and provided in the paper, though aa positions mentioned refer to isoform 2 (AC Q14526-2) throughout. Dominique.Leprince@ibl.fr Dominique.Leprince@ ibl.fr,Dominique.Leprince@ibl.fr Dominique.Leprince@ ibl.fr Mol. Cell. Biol. (0270-7306) 2002 Only protein-protein interactions imex curation ctbp1_mouse C-terminal-binding protein 1 Ctbp1 protein protein mouse Mus musculus Mouse MGSSHLLNKGLPLGVRPPIMNGPMHPRPLVALLDGRDCTVEMPILKDVATVAFCDAQSTQEIHEKVLNEAVGALMYHTITLTREDLEKFKALRIIVRIGSGFDNIDIKSAGDLGIAVCNVPAASVEETADSTLCHILNLYRRTTWLHQALREGTRVQSVEQIREVASGAARIRGETLGIIGLGRVGQAVALRAKAFGFNVLFYDPYLSDGIERALGLQRVSTLQDLLFHSDCVTLHCGLNEHNHHLINDFTVKQMRQGAFLVNTARGGLVDEKALAQALKEGRIRGAALDVHESEPFSFSQGPLKDAPNLICTPHAAWYSEQASIEMREEAAREIRRAITGRIPDSLKNCVNKDHLTAATHWASMDPAVVHPELNGAAYSRYPPGVVSVAPTGIPAAVEGIVPSAMSLSHGLPPVAHPPHAPSPGQTVKPEADRDHTSDQL hic1_human Hypermethylated in cancer 1 protein HIC1 ZBTB29 Zinc finger and BTB domain-containing protein 29 protein protein human Homo sapiens Human MTFPEADILLKSGECAGQTMLDTMEAPGHSRQLLLQLNNQRTKGFLCDVIIVVQNALFRAHKNVLAASSAYLKSLVVHDNLLNLDHDMVSPAVFRLVLDFIYTGRLADGAEAAAAAAVAPGAEPSLGAVLAAASYLQIPDLVALCKKRLKRHGKYCHLRGGGGGGGGYAPYGRPGRGLRAATPVIQACYPSPVGPPPPPAAEPPSGPEAAVNTHCAELYASGPGPAAALCASERRCSPLCGLDLSKKSPPGSAAPERPLAERELPPRPDSPPSAGPAAYKEPPLALPSLPPLPFQKLEEAAPPSDPFRGGSGSPGPEPPGRPDGPSLLYRWMKHEPGLGSYGDELGRERGSPSERCEERGGDAAVSPGGPPLGLAPPPRYPGSLDGPGAGGDGDDYKSSSEETGSSEDPSPPGGHLEGYPCPHLAYGEPESFGDNLYVCIPCGKGFPSSEQLNAHVEAHVEEEEALYGRAEAAEVAAGAAGLGPPFGGGGDKVAGAPGGLGELLRPYRCASCDKSYKDPATLRQHEKTHWLTRPYPCTICGKKFTQRGTMTRHMRSHLGLKPFACDACGMRFTRQYRLTEHMRIHSGEKPYECQVCGGKFAQQRNLISHMKMHAVGGAAGAAGALAGLGGLPGVPGPDGKGKLDFPEGVFAVARLTAEQLSLKQQDKAAAAELLAQTTHFLHDPKVALESLYPLAKFTAELGLSPDKAAEVLSQGAHLAAGPDGRTIDRFSPT g3hk86_crigr I79_011104 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MNGPLHPRPLVALLDGRDCTVEMPILKDVATVAFCDAQSTQEIHEKVLNEAVGALMYHTITLTREDLEKFKALRIIVRIGSGFDNIDIKSAGDLGIAVCNVPAASVEETADSTLCHILNLYRRTTWLHQALREGTRVQSVEQIREVASGAARIRGETLGIIGLGRVGQAVALRAKAFGFNVLFYDPYLSDGIERALGLQRVSTLQDLLFHSDCVTLHCGLNEHNHHLINDFTVKQMRQGAFLVNTARGGLVDEKALAQALKEGRIRGAALDVHESEPFSFSQGPLKDAPNLICTPHAAWYSEQASIEMREEAAREIRRAITGRIPDSLKNCVNKDHLTAATHWASMDPAVVHPELNGAAYSRYPPGVVSVAPTGIPAAVEGIVPSAMSLSHGLPPVAHPPHAPSPGQTVKPEADRDHTSDQL hic1-ctbp1 1231980 n/a 1231986 unspecified role unspecified role prey prey n/a 1231987 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 2 hic1-hic1-1 1231980 n/a 1231987 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1231987 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 2 hic1-ctbp1-1 1231981 n/a 1231986 unspecified role unspecified role prey prey n/a 1231987 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 3B A HIC1 bait construct including the BTB/POZ domain shows only a low interaction probably due to a masked CtB1 interaction motif. ctbp1-hic1 1231982 n/a 1231986 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1231987 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 5A mCtBP1 interacts with Flag-HIC1 but not with Flag-HIC1 Delta-GLDLSKK. hic1-ctbp1-2 1231983 n/a 1231987 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1231986 unspecified role unspecified role prey prey physical association physical association false false false 5B mCtBP1 protein specifically interacts with the HIC1 Delta-GLDLSKK protein, albeit with a much lower affinity than with the full-length HIC1 protein. ctbp1-hic1-1 1231984 n/a 1231986 unspecified role unspecified role neutral component neutral component n/a 1231987 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false colocalization colocalization false false false 6 hic1-ctbp1-10 1231985 n/a 1231988 unspecified role unspecified role prey prey n/a 1231987 unspecified role unspecified role bait bait n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 7b