UniProt UniProt http://www.uniprot.org tanaka-2001-2 Establishment and maintenance of sister chromatid cohesion in fission yeast by a unique mechanism. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Tanaka K., Hao Z., Kai M., Okayama H. Fig. 7B okayama@m.u-tokyo.ac.jp EMBO J. (0261-4189) 2001 Only protein-protein interactions imex curation tanaka-2001-1 Establishment and maintenance of sister chromatid cohesion in fission yeast by a unique mechanism. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast fluorescence imaging fluorescence microscopy fluorescence imaging anti tag immunost anti tag immunostaining anti tag immunost Tanaka K., Hao Z., Kai M., Okayama H. okayama@m.u-tokyo.ac.jp EMBO J. (0261-4189) 2001 Only protein-protein interactions imex curation tanaka-2001-3 Establishment and maintenance of sister chromatid cohesion in fission yeast by a unique mechanism. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Tanaka K., Hao Z., Kai M., Okayama H. okayama@m.u-tokyo.ac.jp EMBO J. (0261-4189) 2001 Only protein-protein interactions imex curation pds5_schpo Sister chromatid cohesion protein pds5 pds5 Precocious dissociation of sisters protein 5 SPAC110.02 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MIKLQFQRNIVPTHDNPLTTSEILKRLRDLLGELTSLSQDTIDRDSVLPVARSLVNNNLLHHKDKGIRSYTLCCIVELLRLCAPDAPFTLSQLEDIFQVILKILSGLMNQESTYYPQIYEILESLSNVKSAVLIVDLPNAEEFLVNIFRLFFDLARKGTTKNVEFYMLDIINQLINEINTIPAAALNILFAQLISGKGVRQTIGSSDSTNHGPAFQLARNIFHDSADRLQRYVCQYFSDIIFDSRDSLSDSMTTPEFIFSHNLVLQLWKYAPTTLLNIIPQFENELQAEQTSVRLVAIETVGLMLQDNAIWSDYPRVWSAFCGRLNDKSVACRIKCIEVASNALQNSLATSEIIENVVQMLQSKLADTDEKVRVATLKTIEQLTFETFKMQFSVQALKLMGDRLRDRKLNVRLQAIRTLSQIYNRAYQDLIDGVEYSIQMFSWIPSSLLEVFYVNDETTNAAVEICMAELVLQYLSSDTQTRLNRLFLSIKYFSEKAMRVFILLLQRQVKYSELLNYYIECCKNYNGGVMDNDEESITNKLKKVIDIISSKSSNPTLTEATFRKFAELNDRQSYKMLLQTFSIKSEYQVVLKSIKYLFKRVSETLSTASLECFRIFVYRSALFAFNKSNVHEIIQLLNEPVKYHNFLKPSEALLQHLPLIHPNIYGEVVIEVENIIVSSGIESDPKVIKALSQFSKRKKNFSIQTTTAEILRKLCLHGTQEQAKQAATIIAITETKEFKLDMITNIVENLEYNGGLPVRLMTLGQLFLYTLEEVEKVADQVTEFLVKKVIQRFPEKYDDTHNDEEWCTYEKLDNLTMCKVLAIRVLVNRLRAAAGGTEALNIGAPIIKLLKVLLMADGELSPFKNTPKISRAYLRLTASKYFLKLCSIPFYAEHIDFSSYVQISLLCQDENFDVRNLFLTKLQKQLQLKKLPISYYPLLFLTAVDPEEEIKTKASIWIRSQVAFFQKTHDFTMEYVATYLIHLLSHHPDISSIESENSLDFIAYIRFYVDTVVNSENVPIVFHLMQRIKQSYDVIEDGNNYIYVLSDMAQKILQVKSQNFGWSLTTYPKQIKLPYEILRPIPSIDEKKRIFNKIFITPKMESQIEHAIRTPVSSFAKQTTNKHANLKQKKTHSSKSDKKSSRRRKNEKRRKLNEQNPNIRNVPERSSSRFQGIRINYSEAPSSSEEISEEEEEISEEDFDEIEDL eso1_schpo N-acetyltransferase eso1 eso1 ECO1 homolog Sister chromatid cohesion protein eso1 SPBC16A3.11 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MELGKSKFSWKDLQYCDKAGTQNSPLRVVAHIDQDAFYAQVESVRLGLDHSVPLAVQQWQGLIAVNYAARAANISRHETVTEAKKKCPELCTAHVKTWKAGESEAKYHENPNPNYYKTCLDPYRHESVKILNIIKKHAPVVKKASIDECFIELTSDVKRIVLEEYPYLKIPSEDSNVALPQAPVLLWPAEFGMVIEEEVVDRTKEDYERDWDDVFLFYAAKIVKEIRDDIYLQLKYTCSAGVSFNPMLSKLVSSRNKPNKQTILTKNAIQDYLVSLKITDIRMLGGKFGEEIINLLGTDSIKDVWNMSMDFLIDKLGQTNGPLVWNLCHGIDNTEITTQVQIKSMLSAKNFSQQKVKSEEDAINWFQVFASDLRSRFLELEGMRRPKTICLTVVSRFLRKSRSSQIPMNVDISTQFIVEATSKLLRQLQQEFDVYPISNLSISFQNIIEVDRNSRGIEGFLKKSNDEIYMSTSVSPSIEGRAKLLNENMRENNSFELSSEKDIKSPKRLKRGKGKGIFDMLQQTAVSKPTENSADETYTCEECEQKITLSERNEHEDYHIALSISRKERYNNLVPPSHDKPKQVKPKTYGRKTGSKHYAPLSDETNNKRAFLDAFLGNGGNLTPNWKKQTPKAISNSSDNMTQLHLDLANSTVTCSECSMEYNSTSEEDILLHSRFHSRVLGGVTVSFQCSPIYRVNYGLSSDCIYSINSESSLIDQRKAEEALSFVNNELSSEPIETIGVDKYTTFLFISDKKCVGLLLAERISSAYIVDELELNNNNSTSSAVYIKNENLRKGFVLGISRIWVSASRRKQGIASLLLDNALKKFIYGYVISPAEVAFSQPSESGKQFIISWHRSRNNGSSKSLRYAVYES rad21_schpo Cohesin subunit rad21 rad21 SPCC338.17c Double-strand-break repair protein rad21 SCC1 homolog protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MFYSEAILSKKGPLAKVWLAAHWEKKLSKVQTLHTSIEQSVHAIVTEETAPMALRLSGQLMLGVVRIYSRKARYLLEDCTEALMRLKMSFQPGQVDMIEPATALQSLKGKDAVTQSANLTLPETITEFDLLVPDSTFDFQWSQLLRTPSRSSNTLELHSLPISSSPSFPSSQLSIEAGRNAQVESGFSLGESFAHVGNDMQFHLPISNSGAATPRSVHSDNQSQISIEVGRDAPAAAATDLSGIIGPQMTKSPASSVTHFSTPSMLPIGGTSLDDELLAPVDDLNLDLGLDDLLGDEQGANAPAIEADEQAETSSIHLPSDIMEDDSSRPAAAGVEEGQVVESATAPQQEKINPQKTVRRQRAIIDPVTELSSKQMKKQLADTSSITSPLCLNTSSIVFNATVNFTRNGKFNTSIFSSNLNPKVNELLQADFKQAILRKRKNESPEEVEPAKHQRTDTSTENQETAEVLDPEEIAAAELANITEAAIATLPQETVVQPEGEAPELGSPMGFPVTALESADDSLFDAPPVMLDEADLLGSERLDSSVSEALPSSQTAKDSLRNKWDPYTEGEKVSFQTLSAGCNREEAVQLFFDVLVLATKDVISVKQDVAIQNEITLTAKRGMLLSSL eso1-pds5 1021493 n/a 1021496 unspecified role unspecified role prey prey n/a 1021497 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 7B pds5-eso1 1021493 n/a 1021496 unspecified role unspecified role bait bait n/a 1021497 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 7B pds5-rad21 1021494 n/a 1021498 unspecified role unspecified role neutral component neutral component c-ter ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1021496 unspecified role unspecified role neutral component neutral component c-ter myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false colocalization colocalization false false false Fig. 4D rad21-pds5-1 1021495 n/a 1021496 unspecified role unspecified role prey prey c-ter myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1021498 unspecified role unspecified role bait bait c-ter ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig. 4J