IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ usui-1998-1 Complex formation and functional versatility of Mre11 of budding yeast in recombination. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Usui T., Ohta T., Oshiumi H., Tomizawa J., Ogawa H., Ogawa T. tsuohta@lab.nig.ac.jp Cell (0092-8674) 1998 Only protein-protein interactions imex curation usui-1998-2 Complex formation and functional versatility of Mre11 of budding yeast in recombination. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Usui T., Ohta T., Oshiumi H., Tomizawa J., Ogawa H., Ogawa T. tsuohta@lab.nig.ac.jp Cell (0092-8674) 1998 Only protein-protein interactions imex curation usui-1998-3 Complex formation and functional versatility of Mre11 of budding yeast in recombination. in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Usui T., Ohta T., Oshiumi H., Tomizawa J., Ogawa H., Ogawa T. Reaction mixture contained Mre11 and one of DNA substrates at 30C. Products were separated by gel electrophoresis followed by staining with ethidium bromide. Exonuclease activity was measured by densitometric analysis. tsuohta@lab.nig.ac.jp Cell (0092-8674) 1998 Only protein-protein interactions imex curation usui-1998-4 Complex formation and functional versatility of Mre11 of budding yeast in recombination. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Usui T., Ohta T., Oshiumi H., Tomizawa J., Ogawa H., Ogawa T. tsuohta@lab.nig.ac.jp Cell (0092-8674) 1998 Only protein-protein interactions imex curation mre11_yeast Double-strand break repair protein MRE11 YM9959.06C YMR224C MRE11 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDYPDPDTIRILITTDNHVGYNENDPITGDDSWKTFHEVMMLAKNNNVDMVVQSGDLFHVNKPSKKSLYQVLKTLRLCCMGDKPCELELLSDPSQVFHYDEFTNVNYEDPNFNISIPVFGISGNHDDASGDSLLCPMDILHATGLINHFGKVIESDKIKVVPLLFQKGSTKLALYGLAAVRDERLFRTFKDGGVTFEVPTMREGEWFNLMCVHQNHTGHTNTAFLPEQFLPDFLDMVIWGHEHECIPNLVHNPIKNFDVLQPGSSVATSLCEAEAQPKYVFILDIKYGEAPKMTPIPLETIRTFKMKSISLQDVPHLRPHDKDATSKYLIEQVEEMIRDANEETKQKLADDGEGDMVAELPKPLIRLRVDYSAPSNTQSPIDYQVENPRRFSNRFVGRVANGNNVVQFYKKRSPVTRSKKSGINGTSISDRDVEKLFSESGGELEVQTLVNDLLNKMQLSLLPEVGLNEAVKKFVDKDEKTALKEFISHEISNEVGILSTNEEFLRTDDAEEMKALIKQVKRANSVRPTPPKENDETNFAFNGNGLDSFRSSNREVRTGSPDITQSHVDNESRITHISQAESSKPTSKPKRVRTATKKKIPAFSDSTVISDAENELGDNNDAQDDVDIDENDIIMVSTDEEDASYGLLNGRKTKTKTRPAASTKTASRRGKGRASRTPKTDILGSLLAKKRK D58433E32E852B73 rad50_yeast DNA repair protein RAD50 RAD50 YNL250W N0872 153 kDa protein protein protein yeast Saccharomyces cerevisiae Baker's yeast MSAIYKLSIQGIRSFDSNDRETIEFGKPLTLIVGMNGSGKTTIIECLKYATTGDLPPNSKGGVFIHDPKITGEKDIRAQVKLAFTSANGLNMIVTRNIQLLMKKTTTTFKTLEGQLVAINNSGDRSTLSTRSLELDAQVPLYLGVPKAILEYVIFCHQEDSLWPLSEPSNLKKKFDEIFQAMKFTKALDNLKSIKKDMSVDIKLLKQSVEHLKLDKDRSKAMKLNIHQLQTKIDQYNEEVSEIESQLNEITEKSDKLFKSNQDFQKILSKVENLKNTKLSISDQVKRLSNSIDILDLSKPDLQNLLANFSKVLMDKNNQLRDLETDISSLKDRQSSLQSLSNSLIRRQGELEAGKETYEKNRNHLSSLKEAFQHKFQGLSNIENSDMAQVNHEMSQFKAFISQDLTDTIDQFAKDIQLKETNLSDLIKSITVDSQNLEYNKKDRSKLIHDSEELAEKLKSFKSLSTQDSLNHELENLKTYKEKLQSWESENIIPKLNQKIEEKNNEMIILENQIEKFQDRIMKTNQQADLYAKLGLIKKSINTKLDELQKITEKLQNDSRIRQVFPLTQEFQRADLEMDFQKLFINMQKNIAINNKKMHELDRRYTNALYNLNTIEKDLQDNQKSKEKVIQLLSENLPEDCTIDEYNDVLEETELSYKTALENLKMHQTTLEFNRKALEIAERDSCCYLCSRKFENESFKSKLLQELKTKTDANFEKTLKDTVQNEKEYLHSLRLLEKHIITLNSINEKIDNSQKCLEKAKEETKTSKSKLDELEVDSTKLKDEKELAESEIRPLIEKFTYLEKELKDLENSSKTISEELSIYNTSEDGIQTVDELRDQQRKMNDSLRELRKTISDLQMEKDEKVRENSRMINLIKEKELTVSEIESSLTQKQNIDDSIRSKRENINDIDSRVKELEARIISLKNKKDEAQSVLDKVKNERDIQVRNKQKTVADINRLIDRFQTIYNEVVDFEAKGFDELQTTIKELELNKAQMLELKEQLDLKSNEVNEEKRKLADSNNEEKNLKQNLELIELKSQLQHIESEISRLDVQNAEAERDKYQEESLRLRTRFEKLSSENAGKLGEMKQLQNQIDSLTHQLRTDYKDIEKNYHKEWVELQTRSFVTDDIDVYSKALDSAIMKYHGLKMQDINRIIDELWKRTYSGTDIDTIKIRSDEVSSTVKGKSYNYRVVMYKQDVELDMRGRCSAGQKVLASIIIRLALSETFGANCGVIALDEPTTNLDEENIESLAKSLHNIINMRRHQKNFQLIVITHDEKFLGHMNAAAFTDHFFKVKRDDRQKSQIEWVDINRVTY 58A0AA173AC5677E xrs2_yeast DNA repair protein XRS2 XRS2 D9481.13 YDR369C protein protein yeast Saccharomyces cerevisiae Baker's yeast MWVVRYQNTLEDGSISFISCCLQAFKTYSIGRSSKNPLIIKNDKSISRQHITFKWEINNSSDLKHSSLCLVNKGKLTSLNKKFMKVGETFTINASDVLKSTIIELGTTPIRIEFEWINEVWNIPPHLTQFRTMLSEYGISTEISINDIPANLMISDYPKSEDNSIRELYALVSTIPMKKSRFLMELCNTLLPTSKTNLKFDEMWNDMISNPEYNVFDFDPNILLSKFMRLNNIRVLTTIKSEPRLSSLLRTFNINLFAFDNIDSLYKYVDSLEASTEYLILTTTDKKENGKILCTIKTMLTSIIDGTLSAVINMKGASSRTLDNGKFDQISEGMSTILKTSRAPEVEASPVVSKKRKLNRRRVLPLDSLDFFAGGLSTKTLSENRSLTDAKRLNCGAESKTVISSPNIAEADEKHAPFLQNALKPTEDIGKKSGHSSPGAIIVSSPNLGTVNTSEDSLDKSLQSHKLPQPSLPEVAGIGSQTISSNSADYETAAVNSMDDAEVTKNFRVNHHQNIEQPSKNIRKLSNYSREISSPLQENCKSPVKELSIKEKSGTPHAFVEAIQETKNREVKRVKSTIVELKDEELSEEAINQLKNLAIVEPSNNLLRKSFDSEGNKTSRTTEKWENSLMEPEWHKRKNFKTFVKVRPKSKAHKEEGKNNTQSSDFIRNAAFLITRNYVPLKKYSKKDTTTKWGTEENEDMFALTEMERFGSNTFMSDNINSNTIQKRSQALNRFTNEDSSNEGEEDSFSFSRCSGTAASVQPLKNKIFITDEDDLGDIDDKSDRLNHRENNRNLFVVKEMNLRPNLSEECSKQSRHSRSATSRSRGSFGASNNGDGDDDDDDGPKFTFKRRKG 833DAD6D20F6693E m13 Phage M13 genome dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid bpm13 Enterobacteria phage M13 Bacteriophage M13 mre11-rad50-1 Interaction between MRE11, RAD50 and XRS2 922806 n/a 922810 unspecified role unspecified role bait bait n/a 922811 unspecified role unspecified role prey prey n/a 922812 unspecified role unspecified role prey prey association association false false false Two phospho-forms of Xrs2 were formed. Fig. 1A mre11-rad50-2 Interaction between MRE11 and RAD50 922806 n/a 922810 unspecified role unspecified role bait bait P32829:p.[His213Tyr;Leu225Ile] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n/a 922811 unspecified role unspecified role prey prey physical association physical association false false false Interaction observed in XRS2-/- cells. Fig. 1A, 6B mre11-xrs2-1 Interaction between MRE11 and XRS2 922806 n/a 922810 unspecified role unspecified role bait bait n/a 922812 unspecified role unspecified role prey prey physical association physical association false false false Two phospho-forms of Xrs2 were formed. Fig. 1A Interaction observed in Rad50-/- cells. mre11-rad50-3 Interaction between MRE11, RAD50 and XRS2 922806 n/a 922811 unspecified role unspecified role prey prey n/a 922810 unspecified role unspecified role prey prey n/a 922812 unspecified role unspecified role bait bait association association false false false Two phospho-forms of Xrs2 were observed. Figs. 1B, 1C xrs2-mre11-1 Interaction between MRE11 and XRS2 922806 n/a 922812 unspecified role unspecified role bait bait n/a 922810 unspecified role unspecified role prey prey physical association physical association false false false Two phospho-forms of Xrs2 were observed. Fig. 1B Interaction observed in Rad50-/- cells. mre11-rad50-4 Colocalisation of MRE11 and RAD50 922807 n/a 922810 unspecified role unspecified role neutral component neutral component n/a 922811 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 2A xrs2-mre11-2 Colocalisation of MRE11 and XRS2 922807 n/a 922812 unspecified role unspecified role neutral component neutral component n/a 922810 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 1B mre11-m13 Exonuclease activity of MRE11 922808 n/a 922810 enzyme enzyme neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false s1-s2 region Alters kinetics of interaction binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 922813 enzyme target enzyme target substrate substrate neutral component neutral component enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction false false false M13mp8 circular viral ssDNA was converted to the linear form, and further incubation resulted in extensive degradation of the substrate suggesting 3'-to-5' ssDNA exonuclease activity. Figs. 4, 5C mre11-rad50-5 Interaction between MRE11 and RAD50 922809 n/a 922810 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 922811 unspecified role unspecified role prey prey n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false s1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false s2 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false The S1 and S2 segments bound to Rad50 with similar efficiency showing that Mre11 has two binding sites for the Rad50. Fig. 5B