IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ han-2010-1 Insights into egg coat assembly and egg-sperm interaction from the X-ray structure of full-length ZP3. crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Han L., Monne' M., Okumura H., Schwend T., Cherry AL., Flot D., Matsuda T., Jovine L. Proteins purified by nickel chromatography and identified by anti-tag western blot prior to size exclusion chromatography on Superdex 200 10/300 GL column. luca.jovine@ki.se Cell (0092-8674) 2010 Only protein-protein interactions imex curation han-2010-2 Insights into egg coat assembly and egg-sperm interaction from the X-ray structure of full-length ZP3. in vitro In vitro crosslink cross-linking study crosslink weight by staining molecular weight estimation by staining weight by staining Han L., Monne' M., Okumura H., Schwend T., Cherry AL., Flot D., Matsuda T., Jovine L. Proteins purified by nickel chromatography and identified by anti-tag western blot prior to size exclusion chromatography on Superdex 200 10/300 GL column. luca.jovine@ki.se Cell (0092-8674) 2010 Only protein-protein interactions imex curation han-2010-3 Insights into egg coat assembly and egg-sperm interaction from the X-ray structure of full-length ZP3. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Han L., Monne' M., Okumura H., Schwend T., Cherry AL., Flot D., Matsuda T., Jovine L. Proteins purified by nickel chromatography and identified by anti-tag western blot prior to size exclusion chromatography on Superdex 200 10/300 GL column. luca.jovine@ki.se Cell (0092-8674) 2010 Only protein-protein interactions imex curation zp3_chick Zona pellucida sperm-binding protein 3 ZP3 ZPC Sperm receptor Zona pellucida C protein Zona pellucida glycoprotein 3 protein protein chick Gallus gallus (Chicken) Chicken MQGGRVVLGLLCCLVAGVGSYTPWDISWAARGDPSAWSWGAEAHSRAVAGSHPVAVQCQEAQLVVTVHRDLFGTGRLINAADLTLGPAACKHSSLNAAHNTVTFAAGLHECGSVVQVTPDTLIYRTLINYDPSPASNPVIIRTNPAVIPIECHYPRRENVSSNAIRPTWSPFNSALSAEERLVFSLRLMSDDWSTERPFTGFQLGDILNIQAEVSTENHVPLRLFVDSCVAALSPDGDSSPHYAIIDFNGCLVDGRVDDTSSAFITPRPREDVLRFRIDVFRFAGDNRNLIYITCHLKVTPADQGPDPQNKACSFNKARNTWVPVEGSRDVCNCCETGNCEPPALSRRLNPMERWQSRRFRRDAGKEVAADVVIGPVLLSADPGAVGQQEEGGDGAAVMVPSVGTGLVCVAVAVALAAVGVAVGIARKGCTRTSAAV zp3 Homodimerization of ZP3 997681 n/a 997684 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false undefined mutation asn159gln_arg359_361_362ala mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 direct interaction direct interaction false false false Radius of gyration (Rg) of 32.02 A. Supp. Fig. 1C zp3-1 Homodimerization of ZP3 997682 n/a 997684 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false undefined mutation asn159gln_arg359_361_362ala mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. Stoichiometry: 2.0 direct interaction direct interaction false false false Supp. Fig. 1D zp3-2 Homodimerization of ZP3 997683 n/a 997684 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. Stoichiometry: 2.0 direct interaction direct interaction false false false Supp. Fig. 5, Supp. Table 1 2.0-2.6A R working 21.9%, R free 24.9% (3NK3) Two molecules of ZP3 embrace each other in antiparallel orientation to form a flat, Yin-Yang-shaped homodimer