IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ rocheleau-1999-1 WRM-1 activates the LIT-1 protein kinase to transduce anterior/posterior polarity signals in C. elegans. in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Rocheleau CE., Yasuda J., Shin TH., Lin R., Sawa H., Okano H., Priess JR., Davis RJ., Mello CC. craig.mello@ummed.edu Cell (0092-8674) 1999 Only protein-protein interactions imex curation rocheleau-1999-5 WRM-1 activates the LIT-1 protein kinase to transduce anterior/posterior polarity signals in C. elegans. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Rocheleau CE., Yasuda J., Shin TH., Lin R., Sawa H., Okano H., Priess JR., Davis RJ., Mello CC. craig.mello@ummed.edu Cell (0092-8674) 1999 Only protein-protein interactions imex curation rocheleau-1999-6 WRM-1 activates the LIT-1 protein kinase to transduce anterior/posterior polarity signals in C. elegans. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Rocheleau CE., Yasuda J., Shin TH., Lin R., Sawa H., Okano H., Priess JR., Davis RJ., Mello CC. craig.mello@ummed.edu Cell (0092-8674) 1999 Only protein-protein interactions imex curation rocheleau-1999-7 WRM-1 activates the LIT-1 protein kinase to transduce anterior/posterior polarity signals in C. elegans. caeel-embryo Caenorhabditis elegans embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti tag coip anti tag coimmunoprecipitation anti tag coip monoclonal western monoclonal antibody western blot monoclonal western Rocheleau CE., Yasuda J., Shin TH., Lin R., Sawa H., Okano H., Priess JR., Davis RJ., Mello CC. craig.mello@ummed.edu Cell (0092-8674) 1999 Only protein-protein interactions imex curation wrm1_caeel Armadillo repeat-containing protein wrm-1 wrm-1 Worm armadillo protein 1 B0336.1 protein protein caeel Caenorhabditis elegans MDVDCAETFSQPCTPLNFNPMTPSTSRVSTPVRPSSTMSARQYSGSPFKAQPQNMEPSNSRVQELREAAVGKRSYTNAWMQGTYAPPQMAGQQQRFSRPPSVIGSTMSHMTNMSEMTAYSYGGLSMLSVNTEMGEFNNFVNQAPYQRALTRVSQVSENQDPTNRQYPMTAPEIIENLESTELINQAAAIRALEPIVKAGGMLQTWGPKGAEPIIRALFQVLIPRPVENENVIRKAFEILHHSILLSNKEIRRIDRMFFRLNAALMDPNGPPVFNVPKPYSIYEIVMTRAIQLDTKFESSAMVLLVHLCCKPHFMKIFFGEDETQQSPAHRRLHKIVIEFAIGNLRRPETKSKNKGLCVSIIKNLSNKNATIKDMSERLGVVSLFHQIMQNEVIHEDLLWSTMQALTVFCGDVKNGTHFVQMGGAQVLCGLLSHGSTRLLHELLKCLRRVSDLPAIQEQDMKESIHCIVQLIGCSDVTIVELATGTLRNIGLHNKMNKAFMVQDGVTSHAIAVLRTSEQFTYQPHANIDLYRKQILSIYENCLSVLNNVTSMAPQDIKESAVSACRMISENADSAYVLLHYFNVGNRKCRKLAVTVMKRVIETVPAFADPFVDLLGTTNEPLPILLLQRAFQSLDEWRKTSVEMMNCDGRSAEQRRELDDRRKDHEDIVKRSVGLLTNLCSQANPRFFHSLKLVLTNGTLNPFQWLTHEMSDGILQEWLAFILSICSRDESLQTFMMYRFLEQAKMTEAFFAELKARRQNSNIQTMLSKIIDLGRHQQRIVSQQHQQHQMQHHRQLM nlk_caeel Serine/threonine kinase NLK nlk W06F12.1 lit-1 Nemo-like kinase Loss of intestine protein 1 protein protein caeel Caenorhabditis elegans MVSWGRGKDAYYLYISREQEEDDDDSLSFYSSQKDTEDEFCGCLFPDPEIPGSSSSSGCSSSSTELYDLAAAHAALISRQQQILSQAIPIIPEHQLAAVAAHHQHHQQLHPSVQYQLVAAATHHNHHQPQAAQPHYSAVVPRSDVIQQPPHFALHHHLQNLVQQQQQQQAHHHHQQLVGEMALVSHTHPAAVGSTTCYEKNQQKQQQVQQIPTQPQVAHVSSNAILAAAQPFYPPPVQDSQPDRPIGYGAFGVVWSVTDPRSGKRVALKKMPNVFQNLASCKRVFREIKMLSSFRHDNVLSLLDILQPANPSFFQELYVLTELMQSDLHKIIVSPQALTPDHVKVFVYQILRGLKYLHTANILHRDIKPGNLLVNSNCILKICDFGLARTWDQRDRLNMTHEVVTQYYRAPELLMGARRYTGAVDIWSVGCIFAELLQRKILFQAAGPIEQLQMIIDLLGTPSQEAMKYACEGAKNHVLRAGLRAPDTQRLYKIASPDDKNHEAVDLLQKLLHFDPDKRISVEEALQHRYLEEGRLRFHSCMCSCCYTKPNMPSRLFAQDLDPRHESPFDPKWEKDMSRLSMFELREKMYQFVMDRPALYGVALCINPQSAAYKNFASSSVAQASELPPSPQAW pop1_caeel Protein pop-1 pop-1 W10C8.2 Posterior pharynx defect protein 1 protein protein caeel Caenorhabditis elegans MMADEELGDEVKVFRRDEDADDDPMISGETSEQQLADDKKEAVMEAELDGAGRNPSIDVLKSAFPKVEPMSPSFPGLMSHFSPGYSAAALPMFMPLFMNPYAAALRSPSLMFPMGAMSPTFPMFPPSPVYGAAIAAAAAKQHFENMAPLNMRAGHPMNQMGMPPYMHPSSMAPQNVDRRAQGGGKAKKDDHVKKPLNAFMWFMKENRKALLEEIGNNEKQSAELNKELGKRWHDLSKEEQAKYFEMAKKDKETHKERYPEWSARENYAVNKKKTKKRRDKSIPSENNDQKKCRARFGVNNTEMWCKFCKRKKKCEYATDRSGGSDITDSQDGRGTSGAYSSSSESPSPKANAGIALTTQQQQAAMMHTMLMQMRLGSTTGASTHVPSPLASSSAGRSPLDANASDSESDVEEEEDEQIDPTVMQQTHDMLMQESMCTI wrm1-lit1-1 872100 n/a 872104 enzyme target enzyme target substrate substrate neutral component neutral component n terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 n/a 872105 enzyme enzyme neutral component neutral component Q9U9Y8:p.Lys269Gly Alters kinetics of interaction mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 phosphorylation phosphorylation reaction phosphorylation false false false Fig 3A. wrm1-lit1-2 872100 n/a 872106 enzyme target enzyme target substrate substrate neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 872105 enzyme enzyme neutral component neutral component n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 phosphorylation phosphorylation reaction phosphorylation false false false Fig 3B. wrm1-wrm-1 872100 n/a 872104 stimulator stimulator neutral component neutral component n terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 n/a 872105 putative self putative self putative self putative self Q9U9Y8:p.Lys269Gly Alters kinetics of interaction mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false phosphorylation phosphorylation reaction phosphorylation false false false Fig 3A. wrm1-lit1-3 872101 n/a 872104 unspecified role unspecified role prey prey n terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 872105 enzyme enzyme bait bait physical association physical association false false false Fig 4. wrm1-pop1-1 872101 n/a 872104 unspecified role unspecified role prey prey n/a 872106 unspecified role unspecified role bait bait physical association physical association false false false Fig 4. wrm1-lit1-5 872102 n/a 872104 unspecified role unspecified role bait bait n terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 872105 enzyme enzyme prey prey n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 5 top image. wrm1-lit1-6 872102 n/a 872104 unspecified role unspecified role prey prey n terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 872105 unspecified role unspecified role bait bait n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 5 bottom image. wrm-1-lit1-7 872103 n/a 872105 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 872104 unspecified role unspecified role prey prey physical association physical association false false false Fig 5.