IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ shellman-1998-1 Oligomers of the Cdc7/Dbf4 protein kinase exist in the yeast cell. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Shellman YG., Schauer IE., Oshiro G., Dohrmann P., Sclafani RA. Measurement of beta-galactosidas activity taken as a confidence level. robert.sclafani@uchsc.edu Mol. Gen. Genet. (0026-8925) 1998 Only protein-protein interactions imex curation shellman-1998-2 Oligomers of the Cdc7/Dbf4 protein kinase exist in the yeast cell. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Shellman YG., Schauer IE., Oshiro G., Dohrmann P., Sclafani RA. Measurement of beta-galactosidas activity taken as a confidence level. The tagged and untagged form of Cdc7 are differentiated by molecular weight robert.sclafani@uchsc.edu Mol. Gen. Genet. (0026-8925) 1998 Only protein-protein interactions imex curation shellman-1998-3 Oligomers of the Cdc7/Dbf4 protein kinase exist in the yeast cell. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Shellman YG., Schauer IE., Oshiro G., Dohrmann P., Sclafani RA. Measurement of beta-galactosidas activity taken as a confidence level. 5-20% (w/v) sucrose density gradient robert.sclafani@uchsc.edu Mol. Gen. Genet. (0026-8925) 1998 Only protein-protein interactions imex curation cdc7_yeast Cell division control protein 7 CDC7 OAF2 YDL017W D2855 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTSKTKNIDDIPPEIKEEMIQLYHDLPGIENEYKLIDKIGEGTFSSVYKAKDITGKITKKFASHFWNYGSNYVALKKIYVTSSPQRIYNELNLLYIMTGSSRVAPLCDAKRVRDQVIAVLPYYPHEEFRTFYRDLPIKGIKKYIWELLRALKFVHSKGIIHRDIKPTNFLFNLELGRGVLVDFGLAEAQMDYKSMISSQNDYDNYANTNHDGGYSMRNHEQFCPCIMRNQYSPNSHNQTPPMVTIQNGKVVHLNNVNGVDLTKGYPKNETRRIKRANRAGTRGFRAPEVLMKCGAQSTKIDIWSVGVILLSLLGRRFPMFQSLDDADSLLELCTIFGWKELRKCAALHGLGFEASGLIWDKPNGYSNGLKEFVYDLLNKECTIGTFPEYSVAFETFGFLQQELHDRMSIEPQLPDPKTNMDAVDAYELKKYQEEIWSDHYWCFQVLEQCFEMDPQKRSSAEDLLKTPFFNELNENTYLLDGESTDEDDVVSSSEADLLDKDVLLISE B74BA9144D0EC39F dbf4_yeast DDK kinase regulatory subunit DBF4 DBF4 DNA52 YDR052C YD9609.07C D4205 Dumbbell forming protein 4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVSPTKMIIRSPLKETDTNLKHNNGIAASTTAAGHLNVFSNDNNCNNNNTTESFPKKRSLERLELQQQQHLHEKKRARIERARSIEGAVQVSKGTGLKNVEPRVTPKELLEWQTNWKKIMKRDSRIYFDITDDVEMNTYNKSKMDKRRDLLKRGFLTLGAQITQFFDTTVTIVITRRSVENIYLLKDTDILSRAKKNYMKVWSYEKAARFLKNLDVDLDHLSKTKSASLAAPTLSNLLHNEKLYGPTDRDPRTKRDDIHYFKYPHVYLYDLWQTWAPIITLEWKPQELTNLDELPYPILKIGSFGRCPFIGDRNYDESSYKRVVKRYSRDKANKKYALQLRALFQYHADTLLNTSSVNDQTKNLIFIPHTCNDSTKSFKKWMQEKAKNFEKTELKKTDDSAVQDVRNEHADQTDEKNSILLNETETKEPPLKEEKENKQSIAEESNKYPQRKELAATPKLNHPVLATFARQETEEVPDDLCTLKTKSRQAFEIKASGAHQSNDVATSFGNGLGPTRASVMSKNMKSLSRLMVDRKLGVKQTNGNNKNYTATIATTAETSKENRHRLDFNALKKDEAPSKETGKDSAVHLETNRKPQNFPKVATKSVSADSKVHNDIKITTTESPTASKKSTSTNVTLHFNAQTAQTAQPVKKETVKNSGYCENCRVKYESLEQHIVSEKHLSFAENDLNFEAIDSLIENLRFQI BDB93E72EF2ABC0B cdc7-cdc7-1 Homo-oligermerisation of cdc7 794891 n/a 794894 unspecified role unspecified role prey prey n/a 794894 unspecified role unspecified role bait bait physical association physical association false false false author score author-based confidence author score A score generated by an author, usually only relevant for that particular dataset. 53 Millar units x10E3 Table 4 CDC7 fused to the Gal4 DNA-binding-domain demonstrated a significant level of self-activation but this interaction demonstrated significantly higher levels of beta-galactosidase activity 53 Millar units x10E3 cdc7-dbf4-6 Interaction between cdc7 and dbf4 demonstrated by Y2H 794891 n/a 794894 unspecified role unspecified role prey prey n/a 794895 unspecified role unspecified role bait bait physical association physical association false false false author score author-based confidence author score A score generated by an author, usually only relevant for that particular dataset. 668.1 Millar unit x10E3 Table 4 668.1 Millar unit x10E3 dbf4-dbf4-1 dbf4 and dbf4 794891 n/a 794895 unspecified role unspecified role prey prey n/a 794895 unspecified role unspecified role bait bait physical association physical association false false false author score author-based confidence author score A score generated by an author, usually only relevant for that particular dataset. 53.3 Millar unit x10E3 Table 4 53.3 Millar unit x10E3 cdc7-cdc7-2 Homo-oligermerisation of cdc7 794892 n/a 794894 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 794894 unspecified role unspecified role prey prey physical association physical association false false false Figure 1 cdc7-cdc7-3 Homo-oligermerisation of cdc7 794893 n/a 794894 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Figure 2