UniProt UniProt http://www.uniprot.org wendland-1998-1 Pan1p, yeast eps15, functions as a multivalent adaptor that coordinates protein-protein interactions essential for endocytosis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Wendland B., Emr SD. bwendland@ucsd.edu A Yeast cDNA library was used. J. Cell Biol. (0021-9525) 1998 Only protein-protein interactions imex curation wendland-1998-3 Pan1p, yeast eps15, functions as a multivalent adaptor that coordinates protein-protein interactions essential for endocytosis. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Wendland B., Emr SD. bwendland@ucsd.edu A Yeast cDNA library was used. J. Cell Biol. (0021-9525) 1998 Only protein-protein interactions imex curation wendland-1998-2 Pan1p, yeast eps15, functions as a multivalent adaptor that coordinates protein-protein interactions essential for endocytosis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Wendland B., Emr SD. bwendland@ucsd.edu A Yeast cDNA library was used. J. Cell Biol. (0021-9525) 1998 Only protein-protein interactions imex curation wendland-1998-4 Pan1p, yeast eps15, functions as a multivalent adaptor that coordinates protein-protein interactions essential for endocytosis. in vitro In vitro pull down pull down weight silver stain molecular weight estimation by silver staining weight silver stain Wendland B., Emr SD. bwendland@ucsd.edu A Yeast cDNA library was used. J. Cell Biol. (0021-9525) 1998 Only protein-protein interactions imex curation wendland-1998-5 Pan1p, yeast eps15, functions as a multivalent adaptor that coordinates protein-protein interactions essential for endocytosis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wendland B., Emr SD. bwendland@ucsd.edu A Yeast cDNA library was used. J. Cell Biol. (0021-9525) 1998 Only protein-protein interactions imex curation ap18a_yeast Clathrin coat assembly protein AP180A YAP1801 YHR161C protein protein yeast Saccharomyces cerevisiae Baker's yeast MTTYFKLVKGATKIKSAPPKQKYLDPILLGTSNEEDFYEIVKGLDSRINDTAWTIVYKSLLVVHLMIREGSKDVALRYYSRNLEFFDIENIRGSNGSASGDMRALDRYDNYLKVRCREFGKIKKDYVRDGYRTLKLNSGNYGSSRNKQHSINIALDHVESLEVQIQALIKNKYTQYDLSNELIIFGFKLLIQDLLALYNALNEGIITLLESFFELSHHNAERTLDLYKTFVDLTEHVVRYLKSGKTAGLKIPVIKHITTKLVRSLEEHLIEDDKTHNTFVPVDSSQGSAGAVVAKSTAQERLEQIREQKRILEAQLKNEQVAISPALTTVTAAQSYNPFGTDSSMHTNIPMAVANQTQQIANNPFVSQTQPQVMNTPTAHTEPANLNVPEYAAVQHTVNFNPVQDAGVSAQQTGYYSINNHLTPTFTGAGFGGYSVSQDTTAASNQQVSHSQTGSNNPFALHNAATIATGNPAHENVLNNPFSRPNFDEQNTNMPLQQQIISNPFQNQTYNQQQFQQQKMPLSSINSVMTTPTSMQGSMNIPQRFDKMEFQAHYTQNHLQQQQQQQQQQQQQQQQQPQQGYYVPATAGANPVTNITGTVQPQNFPFYPQQQPQPEQSQTQQPVLGNQYANNLNLIDM pan1_yeast Actin cytoskeleton-regulatory complex protein PAN1 PAN1 MDP3 MIP3 YIR006C YIB6C DIM2 Mitochondrial distribution of proteins protein 3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MYNPYQQQGMGYQQQQQQQQQQPNGFYPQQQQGQSSNQPQGQPQPQQQMAFNQPQATGIGGMPQSFGNSFSSMPQQPQTGYNNNGNNGSVYGNGNFGQQPQQQQQQAKPQHTGYVPNSSMPMMNTTGTMPPPNPAQQPQLQSIQPQGTGYYQAANTANVHSVQPLQSQGTGYYVSTPNLISSNQTQQPLQAQGTGYYQSQPQQVPPPQQAQSLQPLKPQQTGFYLQPQNQAPLEPLKPTATGFVNSFANNGLNNDIKIPAIRLSFITAQDQAKFETLFRSIVTNGSNTVSGANCRKILMRSGLPPSQLARIWTLCDTSKAGELLFPEFALAMHLINDVLQGDTIPYELDSKTKNEVSSFIDAINLSIANQDSSANDAPKTPFDEFITAGVQNLQPQPTGYMPQTSFGIPLQSQITGGGVASALNPQSTGFMAPTTFNMSMNTGTPGLNPQITGGAPASMQPNITGNALQPQTTGMMPQTTGMMPQTTGMMPQTSFGVNLGPQLTGGALQSQYTGGYGSVMPQQSGPASMPNLSFNQQGLQSQLTGLQPQPTGFLPPSNFSATMPLTAQKTGFGNNEIYTKSNFNNNLIDNSSQDKISTEEKSLFYKIFETFDTQNKGLLDSPTAVEIFRKSGLNRADLEQIWNLCDINNTGQLNKQEFALGMHLVYGKLNGKPIPNVLPSSLIPSSTKLLDNLKNQLKTEPTTTKEKPSFGKIDALSYKNNDDDVLPNYRNRRKVYSAKNEEQSSFSSPSAKSVNHSSSTLQTDDISVDKTVEKKTAKPKYAGFSREINLKNIASLENEIKNISNPENCYDSSIPSDLTSRFDAIIAKLPNLFNEISTIDNEITNAKIQLYRKKNPSSIIGSGPNGEITENDRKKAKSRALLRARMSALTGKSTESEDSLSMEDEQQSAEIKRIQQENGKNQEIIKDIRSSISDISASLKSTMTGSNMISNQEFERWEFGIGLEDGVREFLDDLKSNSNKSVTESSPFVPSSTPTPVDDRSSSPSYSQFKTAEERAAYLKEQAKKRMKEKLAKFDKNRRNVTQSSRSISSENSREQPQQIAGSSNLVEPRATPFQEEKYVEVAQPTQPVQSTQPVQPTQPVQPTQPVQPTQPVQPTQPVQPTQPVQNVYNAKQESDDEDEDDEEKRLQEELKRLKLKKKADKEKRLAALRKQIEDAQNESDEEETNGKDNFGGHVNVPQAAPVAPSAAFSQNSTNAPRSVHAAVTPAAGKNSTGLPSTTMGHNPYFKDASASSTSTFDARAAEMQRRIQRGLDEDEDDGWSDEDESNNRVAVDNKVEEAKIGHPDHARAPPVTAAPLPSVTPVPPAVPVPQANTSNEKSSPIPIAPIPPSVTQEPPVPLAPPLPAVDGFQEPPIPSAPAIATAVQKSGSSTPALAGGVLPPPPPLPTQQASTSEPIIAHVDNYNGAEKGTGAYGSDSDDDVLSIPESVGTDEEEEGAQPVSTAGIPSIPPAGIPPPPPLP izh4_yeast ADIPOR-like receptor IZH4 Implicated in zinc homeostasis protein 4 IZH4 YOL101C protein protein yeast Saccharomyces cerevisiae Baker's yeast MVSLTTIEQSPVKCETTTEKESNDTRGTDSNENAETKETKKGFPFHDLAKLQKQYKNKSSRNESLVALIYLLGSMLSFCLLIFFTDFYLIPLFPTTTTMTDYIVFNFYLLNVFVFCMVHFIYHFVKNISLQQHLEHWQKFSYLSNINLLISSQITILYYLFYDYVFFFKIFTLLMNFIGLVAYFFILTDKLISSKRFNKTVFFISVSVVCCSLPLLTAIITFDGLENLKERIKVNAITWELVALVAASIIYVTRFPESLFRRNKKEEGWNHSEYLFHLLISGTAFYHFFILIQSYILMHSSLNQPELINFKS ent1_yeast Epsin-1 ENT1 YDL161W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSKQFVRSAKNLVKGYSSTQVLVRNATSNDNHQVSKDSLIELAEKSYDSADFFEIMDMLDKRLNDKGKYWRHIAKALTVIDYLIRFGSENCVLWCRENLYIIKTLKEFRHEDDEGIDQGQIVRVKAKELTALLSDDERLNEERNMNIKGRNRKGRRRRGTGRSDENDDDLQRAISASRLTAEEDERRRKQDEDYETALQLSKEEEELKRLQDLQRMQQQQGQQQLQQPMYYDIFGNPITPEEYAQFQLQQQQQQQQQQLQQQPMYYDVFGNPITPEELAQFQQQQQLQEQQYLASMQQQQQAMSNNPFAKSEQSSSSPKRNQLVAASSPQQLQQQKQQEPLIQNRTGNQSMTDKYSKLNELLATGTGIDTFGNVGEARIPAQHTKTGTFINSQGTGYRQVSDDPNHNPFLNSQYTGLPSTSVVPTQTGYGFGNQSQQQSQNNGSNNRGYTLIDL 37447FB4A6449256 yo105_yeast Uncharacterized protein YOR105W O3217 YOR105W protein protein yeast Saccharomyces cerevisiae Baker's yeast MKKKISKVAALSRNMMLEIRLSKMRPILVATTLMLLHNLQFLTSAIMARTIITIIITSIITSRETVTILPPLIIEVHIQPKIINHIRDINQTKAIDITNEVNIYSPFF clh_yeast Clathrin heavy chain CHC1 YGL206C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDLPIEFTELVDLMSLGISPQFLDFRSTTFESDHFVTVRETKDGTNSVAIVDLAKGNEVTRKNMGGDSAIMHPSQMVISVRANGTIVQIFNLETKSKLKSFTLDEPVIFWRWLSETTLGFVTARSILTSNVFDGNVNAKPQLLTLRHANLNNTQIINFVANKNLDWFAVVGILQENGRIAGRIQLFSKQRNISQAIDGHVAIFTNILLEGNGSTPVQVFVTGNRNATTGAGELRIIEIDHDASLPSQYQKETTDIFFPPDATNDFPIAVQVSEKYGIIYLLTKYGFIHLYELETGTNLFVNRITAESVFTAAPYNHENGIACINKKGQVLAVEISTSQIVPYILNKLSNVALALIVATRGGLPGADDLFQKQFESLLLQNDYQNAAKVAASSTSLRNQNTINRLKNIQAPPGAISPILLYFSTLLDKGKLNKEETIELARPVLQQDRKQLFEKWLKEDKLECSEELGDIVKPFDTTLALACYLRAGAHAKVISCLAELQQFEKIIPYCQKVGYQPNFLVLISSLIRSSPDRASEFAVSLLQNPETASQIDIEKIADLFFSQNHIQQGTSLLLDALKGDTPDQGHLQTRVLEVNLLHAPQVADAILGNNIFSHYDKPTIASLSEKAGLYQRALENYTDIKDIKRCVVHTNALPIDWLVGYFGKLNVEQSLACLKALMDNNIQANIQTVVQVATKFSDLIGPSTLIKLFEDYNATEGLYYYLASLVNLTEDKDVVYKYIEAAAKMKQYREIERIVKDNNVYDPERVKNFLKDANLEDQLPLVIVCDRFDFVHEMILYLYKSQNLKFIETYVQQVNPSKTAQVVGALLDMDCDEAFIQSLLQSVLGQVPINELTTEVEKRNRLKILLPFLEQSLSQGIQDQAVYNALAKIYIDSNNSPEKFLKENDQYDTLDVGHYCEKRDPYLAYIAYEKGQNDDDLIRITNENSMYKYQARYLLERSDLDLWNKVLNQENIHRRQLIDSVISVGIPELTDPEPVSLTVQAFMTNGLKLELIELLEKIILEPSPFNENVALQGLLLLSAIKYEPTKVSSYIEKLDNYDADEIAPLCIEHDLKEEAFEIYDKHEMYGKALKVLIEDIMSLDRAASYADKINTPELWSQIGTAQLDGLRIPDAIESYIKAEDPSNYENVIDIAEQAGKYEELIPFLLMARKTLKEPKIDGALILAYAELNKIHEIENLLAGSNVANLDHVGDKLFENKEYKAARLCYSAVSNYSKLASTLVYLGDYQAAVDTARKASNIKVWKLVNDACIEKKEFKLAQICGLNLIVHAEELDELVERYESNGYFEELISLFEAGLGLERAHMGMFTELAILYSKYEPDKTFEHLKLFWSRINIPKVIRAVEQAHLWSELVFLYAHYDEWDNAALTLIEKSTKDLDHAYFKEVVVKVSNLEIYYKAINFYVKFHPSLLVDLLTSLTPRLDIPRTVKIFSKSDNLPLIKPFLINVLPKNNSVVNQAYHDLMIEEEDYKALQDAVDSYDKFDQLGLASRLESHKLIFFKKIGALLYRRNKKWAKSLSILKEEKLWKDAIETAAISQDPKVVEALLTYFVETGNREGFVALLYAAYNLVRIEFVLEISWMNSLEDYIKPFEISIKKEQNDSIKKITEELAKKSGSNEEHKDGQPLMLMNSAMNVQPTGF 622F3083DF2FE315 clc1_yeast Clathrin light chain CLC1 YGR167W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSEKFPPLEDQNIDFTPNDKKDDDTDFLKREAEILGDEFKTEQDDILETEASPAKDDDEIRDFEEQFPDINSANGAVSSDQNGSATVSSGNDNGEADDDFSTFEGANQSTESVKEDRSEVVDQWKQRRAVEIHEKDLKDEELKKELQDEAIKHIDDFYDSYNKKKEQQLEDAAKEAEAFLKKRDEFFGQDNTTWDRALQLINQDDADIIGGRDRSKLKEILLRLKGNAKAPGA ap18b_yeast Clathrin coat assembly protein AP180B G8610 YGR241C YAP1802 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSLYTKLVKGATKIKMAPPKQKYVDPILSGTSSARGLQEITHALDIRLSDTAWTIVYKALIVLHLMIQQGEKDVTLRHYSHNLDVFQLRKISHTTKWSSNDMRALQRYDEYLKTRCEEYGRLGMDHLRDNYSSLKLGSKNQLSMDEELDHVESLEIQINALIRNKYSVSDLENHLLLYAFQLLVQDLLGLYNALNEGVITLLESFFELSIEHAKRTLDLYKDFVDMTEYVVRYLKIGKAVGLKIPVIKHITTKLINSLEEHLREETKRQRGEPSEPQQDRKPSTAISSTSSHNNNSNDKNKSIAQKKLEQIREQKRLLEQQLQNQQLLISPTVPQDAYNPFGSQQQDLNNDTFSFEPTQPQMTAQVPQPTANPFLIPQQQQQALQLTSASTMPQPSEIQITPNLNNQQTGMYASNLQYTPNFTGSGFGGYTTTENNAIMTGTLDPTKTGSNNPFSLENIAREQQQQNFQNSPNPFTLQQAQTTPILAHSQTGNPFQAQNVVTSPMGTYMTNPVAGQLQYASTGAQQQPQMMQGQQTGYVMVPTAFVPINQQQQQQQHQQENPNLIDI pan1-yap1801-1 790548 n/a 790553 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 790554 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false pan1-izh4-1 790548 n/a 790554 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 790555 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false pan1-ent1-1 790548 n/a 790556 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 790554 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false pan1-yor105w-1 790548 n/a 790557 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 790554 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false yap1801-pan1-1 790549 n/a 790553 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 790554 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false Fig 2B yap1801-chc1-1 790549 n/a 790558 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast n/a 790553 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig 2D yap1801-clc1-1 790549 n/a 790559 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast n/a 790553 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig 2D pan1-yap1801-2 790550 n/a 790554 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 790553 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table II pan1-yap1802-1 790550 n/a 790560 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 790554 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table II yap1801-chc1-2 790551 n/a 790558 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast n/a 790553 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig 2C pan1-yap1801-3 790552 n/a 790554 unspecified role unspecified role bait bait n/a 790553 unspecified role unspecified role prey prey physical association physical association false false false Fig 3