IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ daram-1997-2 Tetramerization of the AKT1 plant potassium channel involves its C-terminal cytoplasmic domain. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Daram P., Urbach S., Gaymard F., Sentenac H., Cherel I. The chromatography was carried out under non-denaturing conditions in the presence of lysophosphatidylcholine. EMBO J. (0261-4189) 1997 Only protein-protein interactions imex curation daram-1997-1 Tetramerization of the AKT1 plant potassium channel involves its C-terminal cytoplasmic domain. spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Daram P., Urbach S., Gaymard F., Sentenac H., Cherel I. The chromatography was carried out under non-denaturing conditions in the presence of lysophosphatidylcholine. EMBO J. (0261-4189) 1997 Only protein-protein interactions imex curation akt1_arath Potassium channel AKT1 AKT1 At2g26650 F18A8.2 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MRGGALLCGQVQDEIEQLSRESSHFSLSTGILPSLGARSNRRVKLRRFVVSPYDHKYRIWEAFLVVLVVYTAWVSPFEFGFLRKPRPPLSITDNIVNAFFAIDIIMTFFVGYLDKSTYLIVDDRKQIAFKYLRSWFLLDLVSTIPSEAAMRISSQSYGLFNMLRLWRLRRVGALFARLEKDRNFNYFWVRCAKLVCVTLFAVHCAACFYYLIAARNSNPAKTWIGANVANFLEESLWMRYVTSMYWSITTLTTVGYGDLHPVNTKEMIFDIFYMLFNLGLTAYLIGNMTNLVVHGTSRTRNFRDTIQAASNFAHRNHLPPRLQDQMLAHLCLKYRTDSEGLQQQETLDALPKAIRSSISHFLFYSLMDKVYLFRGVSNDLLFQLVSEMKAEYFPPKEDVILQNEAPTDFYILVNGTADLVDVDTGTESIVREVKAGDIIGEIGVLCYRPQLFTVRTKRLCQLLRMNRTTFLNIIQANVGDGTIIMNNLLQHLKEMNDPVMTNVLLEIENMLARGKMDLPLNLCFAAIREDDLLLHQLLKRGLDPNESDNNGRTPLHIAASKGTLNCVLLLLEYHADPNCRDAEGSVPLWEAMVEGHEKVVKVLLEHGSTIDAGDVGHFACTAAEQGNLKLLKEIVLHGGDVTRPRATGTSALHTAVCEENIEMVKYLLEQGADVNKQDMHGWTPRDLAEQQGHEDIKALFREKLHERRVHIETSSSVPILKTGIRFLGRFTSEPNIRPASREVSFRIRETRARRKTNNFDNSLFGILANQSVPKNGLATVDEGRTGNPVRVTISCAEKDDIAGKLVLLPGSFKELLELGSNKFGIVATKVMNKDNNAEIDDVDVIRDGDHLIFATDS akt1-4 855312 n/a 855314 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false n/a 855314 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false 855319 855317 physical association physical association false false false Figure 4 Figure 5 akt1-5 855312 n/a 855314 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false n/a 855314 unspecified role unspecified role prey prey c-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false 855324 855322 physical association physical association false false false Figure 4 Figure 5 akt1-3 855312 n/a 855314 unspecified role unspecified role bait bait c-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false n/a 855314 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false 855329 855327 physical association physical association false false false Figure 4 Figure 5 akt1 855313 n/a 855314 unspecified role unspecified role neutral component neutral component c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 4.0 physical association physical association false false false Figure 1 Figure 2B akt1-2 855313 n/a 855314 unspecified role unspecified role neutral component neutral component Stoichiometry: 2.0 physical association physical association false false false Figure 1