IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ conklin-1995-5 14-3-3 proteins associate with cdc25 phosphatases. spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Conklin DS., Galaktionov K., Beach D. HeLa cell two-hybrid cDNA Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1995 Only protein-protein interactions imex curation conklin-1995-2 14-3-3 proteins associate with cdc25 phosphatases. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Conklin DS., Galaktionov K., Beach D. Ras interactions with Raf1 not curated as an unique UniProtKb entry could not be assigned to the Ras entry. HeLa cell two-hybrid cDNA Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1995 Only protein-protein interactions imex curation conklin-1995-1 14-3-3 proteins associate with cdc25 phosphatases. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Conklin DS., Galaktionov K., Beach D. HeLa cell two-hybrid cDNA Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1995 Only protein-protein interactions imex curation conklin-1995-3 14-3-3 proteins associate with cdc25 phosphatases. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Conklin DS., Galaktionov K., Beach D. HeLa cell two-hybrid cDNA Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1995 Only protein-protein interactions imex curation conklin-1995-4 14-3-3 proteins associate with cdc25 phosphatases. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Conklin DS., Galaktionov K., Beach D. HeLa cell two-hybrid cDNA Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1995 Only protein-protein interactions imex curation 1433b_human 14-3-3 protein beta/alpha YWHAB Protein kinase C inhibitor protein 1 Protein 1054 protein protein human Homo sapiens Human MTMDKSELVQKAKLAEQAERYDDMAAAMKAVTEQGHELSNEERNLLSVAYKNVVGARRSSWRVISSIEQKTERNEKKQQMGKEYREKIEAELQDICNDVLELLDKYLIPNATQPESKVFYLKMKGDYFRYLSEVASGDNKQTTVSNSQQAYQEAFEISKKEMQPTHPIRLGLALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDTLNEESYKDSTLIMQLLRDNLTLWTSENQGDEGDAGEGEN raf1_human RAF proto-oncogene serine/threonine-protein kinase RAF1 RAF Proto-oncogene c-RAF Raf-1 protein protein human Homo sapiens Human MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMRESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF mpip1_human M-phase inducer phosphatase 1 Dual specificity phosphatase Cdc25A CDC25A protein protein human Homo sapiens Human MELGPEPPHRRRLLFACSPPPASQPVVKALFGASAAGGLSPVTNLTVTMDQLQGLGSDYEQPLEVKNNSNLQRMGSSESTDSGFCLDSPGPLDSKENLENPMRRIHSLPQKLLGCSPALKRSHSDSLDHDIFQLIDPDENKENEAFEFKKPVRPVSRGCLHSHGLQEGKDLFTQRQNSAPARMLSSNERDSSEPGNFIPLFTPQSPVTATLSDEDDGFVDLLDGENLKNEEETPSCMASLWTAPLVMRTTNLDNRCKLFDSPSLCSSSTRSVLKRPERSQEESPPGSTKRRKSMSGASPKESTNPEKAHETLHQSLSLASSPKGTIENILDNDPRDLIGDFSKGYLFHTVAGKHQDLKYISPEIMASVLNGKFANLIKEFVIIDCRYPYEYEGGHIKGAVNLHMEEEVEDFLLKKPIVPTDGKRVIVVFHCEFSSERGPRMCRYVRERDRLGNEYPKLHYPELYVLKGGYKEFFMKCQSYCEPPSYRPMHHEDFKEDLKKFRTKSRTWAGEKSKREMYSRLKKL q8isr7_spofr protein protein spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm SKAKMQPTHPIRLGLALNFSVFYYEILNSPDKACQL 1433e_human 14-3-3 protein epsilon YWHAE protein protein human Homo sapiens Human MDDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNKEALQDVEDENQ mpip2_human M-phase inducer phosphatase 2 Dual specificity phosphatase Cdc25B CDC25B CDC25HU2 protein protein human Homo sapiens Human MEVPQPEPAPGSALSPAGVCGGAQRPGHLPGLLLGSHGLLGSPVRAAASSPVTTLTQTMHDLAGLGSETPKSQVGTLLFRSRSRLTHLSLSRRASESSLSSESSESSDAGLCMDSPSPMDPHMAEQTFEQAIQAASRIIRNEQFAIRRFQSMPVRLLGHSPVLRNITNSQAPDGRRKSEAGSGAASSSGEDKENDGFVFKMPWKPTHPSSTHALAEWASRREAFAQRPSSAPDLMCLSPDRKMEVEELSPLALGRFSLTPAEGDTEEDDGFVDILESDLKDDDAVPPGMESLISAPLVKTLEKEEEKDLVMYSKCQRLFRSPSMPCSVIRPILKRLERPQDRDTPVQNKRRRSVTPPEEQQEAEEPKARVLRSKSLCHDEIENLLDSDHRELIGDYSKAFLLQTVDGKHQDLKYISPETMVALLTGKFSNIVDKFVIVDCRYPYEYEGGHIKTAVNLPLERDAESFLLKSPIAPCSLDKRVILIFHCEFSSERGPRMCRFIRERDRAVNDYPSLYYPEMYILKGGYKEFFPQHPNFCEPQDYRPMNHEAFKDELKTFRLKTRSWAGERSRRELCSRLQDQ raf1-1433b-1 857594 n/a 857599 unspecified role unspecified role prey prey n/a 857600 unspecified role unspecified role bait bait physical association physical association false false false 4 raf1-1433b-2 857594 n/a 857601 unspecified role unspecified role prey prey n/a 857600 unspecified role unspecified role bait bait n/a 857599 unspecified role unspecified role prey prey physical association physical association false false false 4 raf1-cdc25a 857594 n/a 857601 unspecified role unspecified role prey prey n/a 857600 unspecified role unspecified role bait bait n/a 857602 unspecified role unspecified role prey prey physical association physical association false false false 4 cdc25a-1433b 857594 n/a 857599 unspecified role unspecified role prey prey n/a 857601 unspecified role unspecified role bait bait n/a 857600 unspecified role unspecified role prey prey physical association physical association false false false 4 cdc25a-1433b-4 857594 n/a 857599 unspecified role unspecified role prey prey n/a 857601 unspecified role unspecified role bait bait physical association physical association false false false 4 cdc25a-raf1 857594 n/a 857600 unspecified role unspecified role prey prey n/a 857601 unspecified role unspecified role bait bait physical association physical association false false false 4 1433b-cdc25a-3 857594 n/a 857600 unspecified role unspecified role prey prey n/a 857599 unspecified role unspecified role bait bait n/a 857601 unspecified role unspecified role prey prey physical association physical association false false false 4 1433b-cdc25a-4 857594 n/a 857601 unspecified role unspecified role prey prey n/a 857599 unspecified role unspecified role bait bait physical association physical association false false false 4 1433b-raf1 857594 n/a 857599 unspecified role unspecified role bait bait n/a 857600 unspecified role unspecified role prey prey physical association physical association false false false 4 14-3-3e-cdc25a 857595 n/a 857601 unspecified role unspecified role bait bait n/a 857603 unspecified role unspecified role prey prey physical association physical association false false false table1 14-3-3b-cdc25b 857595 n/a 857599 unspecified role unspecified role prey prey n/a 857604 unspecified role unspecified role bait bait physical association physical association false false false table 1 1433e-cdc25b 857595 n/a 857604 unspecified role unspecified role bait bait n/a 857603 unspecified role unspecified role prey prey physical association physical association false false false table1 1433b-cdc25a 857595 n/a 857599 unspecified role unspecified role prey prey n/a 857601 unspecified role unspecified role bait bait physical association physical association false false false table 1 raf1-1433b 857595 n/a 857600 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 857599 unspecified role unspecified role prey prey physical association physical association false false false table 1 raf1-1433e 857595 n/a 857600 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 857603 unspecified role unspecified role prey prey physical association physical association false false false table 1 cdc25a-14-3-3b 857596 n/a 857601 unspecified role unspecified role bait bait n/a 857599 unspecified role unspecified role prey prey physical association physical association false false false 1 cdc25a-14-3-3e 857596 n/a 857601 unspecified role unspecified role bait bait n/a 857603 unspecified role unspecified role prey prey physical association physical association false false false 1 cdc25b-14-3-3e 857596 n/a 857603 unspecified role unspecified role prey prey n/a 857604 unspecified role unspecified role bait bait physical association physical association false false false 1 cdc25b-14-3-3b 857596 n/a 857604 unspecified role unspecified role bait bait n/a 857599 unspecified role unspecified role prey prey physical association physical association false false false 1 1433b-cdc25a-1 857597 n/a 857601 unspecified role unspecified role prey prey n/a 857599 unspecified role unspecified role bait bait physical association physical association false false false 3b 1433b-cdc25a-2 857598 n/a 857601 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 857599 unspecified role unspecified role prey prey region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false 3A