IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ glynn-2009a-1 Structures of asymmetric ClpX hexamers reveal nucleotide-dependent motions in a AAA+ protein-unfolding machine. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Glynn SE., Martin A., Nager AR., Baker TA., Sauer RT. bobsauer@mit.edu Cell (0092-8674) 2009 Only protein-protein interactions 1 imex curation clpx_ecoli ATP-dependent Clp protease ATP-binding subunit ClpX clpX lopC b0438 JW0428 ATP-dependent unfoldase ClpX protein protein ecoli Escherichia coli (strain K12) MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLCNDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNGDTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVENIIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTVAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKAKSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALTKQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMEDVEKVVIDESVIDGQSKPLLIYGKPEAQQASGE magnesium(2+) Magnesium(2+) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) adenosine 5'-[gamma-thio]triphosphate Adenosine 5'-[gamma-thio]triphosphate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) clx-1 Crystal Structure of Clx hexadimer 865338 n/a 865339 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Glu185Gln mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 6.0 direct interaction direct interaction false false false 4.026 A Working 27.9%, R-factor: 31.3% Table 1 Proteins were covalently linked. clx-2 Crystal Structure of Clx hexadimer with nucleotide and magnesium 865338 n/a 865340 unspecified role unspecified role neutral component neutral component n/a 865341 unspecified role unspecified role neutral component neutral component n/a 865339 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Glu185Gln mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 6.0 direct interaction direct interaction false false false 3.25 A Working 24.5%, R-factor: 28.2% Table 1 Proteins were covalently linked.