mpidb The Microbial Protein Interaction Database pinto_santi-2009-1 Cag3 is a novel essential component of the Helicobacter pylori Cag type IV secretion system outer membrane subcomplex. helpy Helicobacter pylori Campylobacter pylori affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Pinto-Santini DM., Salama NR. nsalama@fhcrc.org J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation pinto_santi-2009-2 Cag3 is a novel essential component of the Helicobacter pylori Cag type IV secretion system outer membrane subcomplex. in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Pinto-Santini DM., Salama NR. nsalama@fhcrc.org J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation pinto_santi-2009-3 Cag3 is a novel essential component of the Helicobacter pylori Cag type IV secretion system outer membrane subcomplex. in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Pinto-Santini DM., Salama NR. nsalama@fhcrc.org J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation o25257_helpy HP_0520 protein protein helpy Helicobacter pylori Campylobacter pylori MADTINTTEATHETKKPNAFVNFFKNNLTDKRYDSLGLIGAGVLCCVLSGAMGIVGIIFVAIGIFLSFSNINLVKLVEKLSKKQSKVPTTVNNETQKSQATSVTNEPTEAKETKD o25277_helpy HP_0545 protein protein helpy Helicobacter pylori Campylobacter pylori MINNNSNKKLRGFFVKVLLSLVVFSSYGLANDDKEAKKEVLEKEKNTPNGLVYTNLDFDSFKATIKNLKDKKVTFKEVNPDIIKDEVFDFVIVNRVLKKIKDLKHYDPVIEKIFDEKGKEMGLNVELQINPEVKDFFTFKSISTTNKQRCFLSLRGETREILCDDKLYNVLLAVFNSYDPNDLLKHISTVESLKKIFYTITCEAVYL o25258_helpy HP_0522 protein protein helpy Helicobacter pylori Campylobacter pylori MFRKLATAVSLIGLLTSNTLYAKEISEADKVIKATKETKETKKEAKRLKKEAKQRQQIPDHKKPQYVSVDDTKTQALFDIYDTLNVNDKSFGDWFGNSALKDKTYLYAMDLLDYNNYLSIENPIIKTRAMGTYADLIIITGSLEQVNGYYNILKALNKRNAKFVLKINENMPYAQATFLRVPKRSDPNAHTLDKGASIDENKLFEQQKKMYFNYANDVICRPDDEVCSPLRDEMVAMPTSDSVTQKPNIIAPYSLYRLKETNNANEAQPSPYATQTAPENSKEKLIEELIANSQLVANEEEREKKLLAEKEKQEAELAKYKLKDLENQKKLKALEAELKKKNAKKPRVVEVPVSPQTSNSDETMRVVKEKENYNGLLVDKETTIKRSYEGTLISENSYSKKTPLNPNDLRSLEEEIKSYYIKSNGLCYTNGINLYVKIKNDPYKEGMLCGYESVQNLLSPLKDKLKYDKQKLQKALLKDSK o25260_helpy HP_0524 protein protein helpy Helicobacter pylori Campylobacter pylori MEDFLYNTLYFIEDYKLVVIFSFIGLIALFFLYKFIKAQKKAFKDKANQPQKKKSFKEIIIDGLKERVKTFGFWLQAILLLSYSFITSGLFFLILLGNFYDDNRSPESDDDLFDIWIYAIQDFPNYYFKALGFSSLKIYGFNISLVVYGSILCSYIFITFFVWFLKYLTRTRDIGANKKVDDLFGSASWETEEKMIKAKLITPNNKKRAFDKREVIVGRRGLGDFIAYAGQAFIGLIAPTRSGKGVGFIMPNMINYPQNIVVFDPKADTMETCGKIREKRFNQKVFIYEPFSLKTHRFNPFAYVDFGNDVVLTEDILSQIDTRLKGHGMVASGGDFSTQIFGLAKLVFPERPNEKDPFFSNQARNLFVINCNIYRDLMWTKKGLEFVKRKKIIMPETPTMFFIGSMASGINLIDEDTNMEKVVSLMEFFGGEEDKSGDNLRVLSPATRNMWNSFKTMGGARETYSSVQGVYTSAFAPYNNAMIRNFTSANDFDFRRLRIDEVSIGVIANPKESTIVGPILELFFNVMIYSNLILPIHDPQCKRSCLMLMDEFTLCGYLETFVKAVGIMAEYNMRPAFVFQSKAQLENDPPLGYGRNGAKTILDNLSLNMYYGINNDNYYEHFEKLSKVLGKYTRQDVSRSIDDNTGKTNTSISNKERFLMTPDELMTMGDELIILENTLKPIKCHKALYYDDPFFTDELIKVSPSLSKKYKLGKVPNQATFYDDLQAAKTRGELSYDKSLVPVGSSEL o25278_helpy HP_0546 protein protein helpy Helicobacter pylori Campylobacter pylori MKFFTRITDSYKKVVVTLGLVVTTNPLMAVTSPAEGVTETKTLVIQIISVLAIVGGCALGVKGIADIWKISDDIKRGQATVFAYAQPIAMLAVAGGIIYLSTKFGFNIGESGGAS cagt_helpy CAG pathogenicity island protein 12 cagT cag12 HP_0532 protein protein helpy Helicobacter pylori Campylobacter pylori MKLRASVLIGATILCLILSACSNYAKKVVKQKNHVYTPVYNELIEKYSEIPLNDKLKDTPFMVQVKLPNYKDYLLDNKQVVLTFKLVHHSKKITLIGDANKILQYKNYFQANGARSDIDFYLQPTLNQKGVVMIASNYNDNPNSKEKPQTFDVLQGSQPMLGANTKNLHGYDVSGANNKQVINEVAREKAQLEKINQYYKTLLQDKEQEYTTRKNNQREILETLSNRAGYQMRQNVISSEIFKNGNLNMQAKEEEVREKLQEERENEYLRNQIRSLLSGK o25263_helpy HP_0528 protein protein helpy Helicobacter pylori Campylobacter pylori MGQAFFKKIVGCFCLGYLFLSSAIEAAALDIKNFNRGRVKVVNKKIAYLGDEKPITIWTSLDNVTVIQLEKDETISYITTGFNKGWSIVPNSNHIFIQPKSVKSNLMFEKEAVNFALMTRDYQEFLKTKKLIVDAPDPKELEEQKKALEKEKEAKEQAQKAQKDKREKRKEERAKNRANLENLTNAMSNPQNLSNNKNLSEFIKQQRENELDQMERLEDMQEQAQANALKQIEELNKKQAEETIKQRAKDKINIKTDKPQKSPEDNSIELSPSDSAWRTNLVVRTNKALYQFILRIAQKDNFASAYLTVKLEYPQRHEVSSVIEEELKKREEAKRQKELIKQENLNTTAYINRVMMASNEQIINKEKIREEKQKIILDQAKALETQYVHNALKRNPVPRNYNYYQAPEKRSKHIMPSEIFDDGTFTYFGFKNITLQPAIFVVQPDGKLSMTDAAIDPNMTNSGLRWYRVNEIAEKFKLIKDKALVTVINKGYGKNPLTKNYNIKNYGELERVIKKLPLVRDK o25276_helpy HP_0543 protein protein helpy Helicobacter pylori Campylobacter pylori MKQSLREQKLLKILENDVLTILDSFSNYLFELREELDFIEEEMEGEITEQNLTALYDFSNFLEDHVNVFYENVLNIDDVKTEHLYSGLIDSLNANLHFVKSFLSNQDLDFRFFKEINDGQDPQKTLSRLIPLQSGKNDASSFKANNSFVSLVYVYVYFMLETIMQSYRILRLLEKPINNNISEDMQNDIENFFVQANFLEYYVQNKIYPTNHAYDFTHLIMDSIIPNWIQTDMSVEAKKKELFEKYFQNIDEVTNKMLDQKNQNXSND q7bk04_helpx Type IV secretion system protein C2R60_04995 cag-alfa cag-alpha C2R96_00505 C2S01_07015 C2S08_08030 C2S19_05495 C2S26_06255 C2S30_00200 C2S37_02945 C2S44_03525 C2S45_02195 D8X56_02785 cagAlpha BZK21_07190 DD743_01745 DD779_02635 DDP48_02060 C2840_02730 virB11 HP0525 cagalpha F7219_04320 F7220_05215 F7213_07340 AC785_03890 ACM37_01770 AEY53_03685 AM497_06540 AM498_02085 AA971_03950 C2R48_00610 C2R51_00230 C2R45_06065 C2R64_07520 C2R66_03525 C2R68_05070 C2R72_03110 C2R62_05930 BGL72_04505 BXP09_03450 CV723_03460 CV726_04895 CV727_03315 CV728_03100 CV729_03635 CV730_03325 D8X81_02825 EC505_06860 EC515_06990 EC525_06690 EC538_06900 EC541_03080 EC547_07075 EC568_02570 EC569_06885 EC572_06310 EC573_06720 EC582_07065 EC594_00565 EC601_02375 ECB88_06645 ECC11_07070 ECC13_06730 ECC14_02950 ECC25_02135 ECC26_02355 ECC29_05940 ECC33_07210 ECC38_04325 ECC39_06170 ECE46_01805 ECE48_01165 EDB76_02000 EGM90_01280 protein protein helpx Helicobacter pylori Campylobacter pylori MTEDRLSAEDKKFLEVERALKEAALNPLRHATEELFGDFLKMENITEICYNGNKVVWVLKNNGEWQPFDVRDRKAFSLSRLMHFARCCASFKKKTIDNYENPILSSNLANGERVQIVLSPVTVNDETISISIRIPSKTTYPHSFFEEQGFYNLLDNKEQAISAIKDGIAIGKNVIVCGGTGSGKTTYIKSIMEFIPKEERIISIEDTEEIVFKHHKNYTQLFFGGNITSADCLKSCLRMRPDRIILGELRSSEAYDFYNVLCSGHKGTLTTLHAGSSEEAFIRLANMSSSNSAARNIKFESLIEGFKDLIDMIVHINHHKQCDEFYIKHR o25270_helpy HP_0537 protein protein helpy Helicobacter pylori Campylobacter pylori MLAKIVFSSLVAFGVLSANVEQFGSFFNEIKKEQEEVAAKEDALKARKKLLNNTHDFLEDLIFRKQKIKELMDHRAKVLSDLENKYKKEKEALEKETRGKILTAKSKAYGDLEQALKDNPLYRKLLPNPYAYVLNQETFTKEDRERLSYYYPQVKTSSIFKKTTATTKDKAQALLQMGVFSLDEEQNKKASRLALSYKQAIEEYSNNVSNLLSRKELDNIDYYLQLERNKFDSKAKDIAQKATNTLIFNSERLAFSMAIDKINEKYLRGYEAFSNLLKNVKDDVELNTLTKNFTNQKLSFAQKQKLCLLVLDSFNFDTQSKKSILKKTNEYNIFVDSDPMMSDKTTMQKEHYKIFNFFKTVVSAYRNNVAKNNPFE caga_helpy Cytotoxicity-associated immunodominant antigen 120 kDa protein CAG pathogenicity island protein 26 cai HP_0547 cagA cag26 protein protein helpy Helicobacter pylori Campylobacter pylori MTNETIDQTRTPDQTQSQTAFDPQQFINNLQVAFIKVDNVVASFDPDQKPIVDKNDRDNRQAFDGISQLREEYSNKAIKNPTKKNQYFSDFIDKSNDLINKDNLIDVESSTKSFQKFGDQRYQIFTSWVSHQKDPSKINTRSIRNFMENIIQPPIPDDKEKAEFLKSAKQSFAGIIIGNQIRTDQKFMGVFDESLKERQEAEKNGGPTGGDWLDIFLSFIFNKKQSSDVKEAINQEPVPHVQPDIATTTTDIQGLPPEARDLLDERGNFSKFTLGDMEMLDVEGVADIDPNYKFNQLLIHNNALSSVLMGSHNGIEPEKVSLLYAGNGGFGDKHDWNATVGYKDQQGNNVATLINVHMKNGSGLVIAGGEKGINNPSFYLYKEDQLTGSQRALSQEEIRNKVDFMEFLAQNNTKLDNLSEKEKEKFQNEIEDFQKDSKAYLDALGNDRIAFVSKKDTKHSALITEFNNGDLSYTLKDYGKKADKALDREKNVTLQGSLKHDGVMFVDYSNFKYTNASKNPNKGVGATNGVSHLEAGFNKVAVFNLPDLNNLAITSFVRRNLENKLTAKGLSLQEANKLIKDFLSSNKELAGKALNFNKAVAEAKSTGNYDEVKKAQKDLEKSLRKREHLEKEVEKKLESKSGNKNKMEAKAQANSQKDEIFALINKEANRDARAIAYTQNLKGIKRELSDKLEKISKDLKDFSKSFDEFKNGKNKDFSKAEETLKALKGSVKDLGINPEWISKVENLNAALNEFKNGKNKDFSKVTQAKSDLENSVKDVIINQKVTDKVDNLNQAVSVAKAMGDFSRVEQVLADLKNFSKEQLAQQAQKNEDFNTGKNSELYQSVKNSVNKTLVGNGLSGIEATALAKNFSDIKKELNEKFKNFNNNNNGLKNSTEPIYAKVNKKKTGQVASPEEPIYTQVAKKVNAKIDRLNQIASGLGGVGQAAGFPLKRHDKVDDLSKVGLSASPEPIYATIDDLGGPFPLKRHDKVDDLSKVGRSRNQELAQKIDNLNQAVSEAKAGFFGNLEQTIDKLKDSTKKNVMNLYVESAKKVPASLSAKLDNYAINSHTRINSNIQNGAINEKATGMLTQKNPEWLKLVNDKIVAHNVGSVSLSEYDKIGFNQKNMKDYSDSFKFSTKLNNAVKDIKSGFTHFLANAFSTGYYCLARENAEHGIKNVNTKGGFQKS o25262_helpy HP_0527 protein protein helpy Helicobacter pylori Campylobacter pylori MNEENDKLETSKKAQQDSPQDLSNEEATEANHFENLLKESKESSDHHLDNPTETQTHFDGDKSEETQTQMDSEGNETSESSNGSLADKLFKKARKLVDNKKPFTQQKNLDEETQELNEEDDQENNEYQEETQTDLIDDETSKKTQQHSPQDLSNEEATEANHFENLLKESKESSDHHLDNPTETQTNFDGDKSEETQTQMDSEGNETSESSNGSLADKLFKKARKLVDNKKPFTQQKNLDEETQELNEEDDQENNEYQEETQTDLIDDETSKKTQQHSPQDLSNEEATEANHFENLLKESKESSDHHLDNPTETQTNFDGDKSEEITDDSNDQEIIKGSKKKYIIGGIVVAVLIVIILFSRSIFHYFMPLEDKSSRFSKDRNLYVNDEIQIRQEYNRLLKERNEKGNMIDKNLFFNDDPNRTLYNYLNIAEIEDKNPLRAFYECISNGGNYEECLKLIKDKKLQDQMKKTLEAYNDCIKNAKTEEERIKCLDLIKDENLKKSLLNQQKVQVALDCLKNAKTDEERNECLKLINDPEIREKFRKELELQKELQEYKDCIKNAKTEAEKNKCLKGLSKEAIERLKQQALDCLKNAKTDEERNECLKNIPQDLQKELLADMSVKAYKDCVSKARNEKEKQECEKLLTPEARKKLEQQVLDCLKNAKTDEERKKCLKDLPKDLQSDILAKESLKAYKDCVSQAKTEAEKKECEKLLTPEAKKLLEEEAKESVKAYLDCVSQAKTEAEKKECEKLLTPEAKKKLEEAKKSVKAYLDCVSRARNEKEKKECEKLLTPEAKKLLEQQALDCLKNAKTDKERKKCLKDLPKDLQKKVLAKESVKAYLDCVSQAKTEAEKKECEKLLTPEARKLLEEAKKSVKAYLDCVSQAKTEAEKKECEKLLTPEARKLLEEXAKESVKAYLDCVSQAKNEAEKKECEKLLTLESKKKLEEAKKSVKAYLDCVSQAKTEAEKKECEKLLTPEAKKLLEQQALDCLKNAKTEADKKRCVKDLPKDLQKKVLAKESLKAYKDCVSKARNEKEKKECEKLLTPEAKKLLEEAKKSVKAYLDCVSQAKTEAEKKECEKLLTPEARKLLEEAKESVKAYKDCVSKARNEKEKKECEKLLTPEAKKLLEQQVLDCLKNAKTEADKKRCVKDLPKDLQKKVLAKESVKAYLDCVSRARNEKEKKECEKLLTPEAKKLLEEAKESLKAYKDCLSQARNEEERRACEKLLTPEARKLLEQEVKKSIKAYLDCVSRARNEKEKKECEKLLTPEARKFLAKQVLNCLEKAGNEEERKACLKNLPKDLQENILAKESLKAYKDCLSQARNEEERRACEKLLTPEARKLLEQEVKKSVKAYLDCVSRARNEKEKKECEKLLTPEARKFLAKELQQKDKAIKDCLKNADPNDRAAIMKCLDGLSDEEKLKYLQEAREKAVADCLAMAKTDEEKRKCQNLYSDLIQEIQNKRTQNKQNQLSKTERLHQASECLDNLDDPTDQEAIEQCLEGLSDSERALILGIKRQADEVDLIYSDLRNRKTFDNMAAKGYPLLPMDFKNGGDIATINATNVDADKIASDNPIYASIEPDIAKQYETEKTIKDKNLEAKLAKALGGNKKDDDKEKSKKSTAEAKAENNKIDKDVAETAKNISEIALKNKKEKSGEFVDENGNPIDDKKKAEKQDETSPVKQAFIGKSDPTFVLAQYTPIEITLTSKVDATLTGIVSGVVAKDVWNMNGTMILLDKGTKVYGNYQSVKGGTPIMTRLMIVFTKAITPDGVIIPLANAQAAGMLGEAGVDGYVNNHFMKRIGFAVIASVVNSFLQTAPIIALDKLIGLGKGRSERTPEFNYALGQAINGSMQSSAQMSNQILGQLMNIPPSFYKNEGDSIKILTMDDIDFSGVYDVKITNKSVVDEIIKQSTKTLSREHEEITTSPKGGN cag3_helpy 867373 n/a 867376 unspecified role unspecified role prey prey n/a 867377 unspecified role unspecified role prey prey n/a 867378 unspecified role unspecified role bait bait n/a 867379 unspecified role unspecified role prey prey n/a 867380 unspecified role unspecified role prey prey n/a 867381 unspecified role unspecified role prey prey n/a 867382 unspecified role unspecified role prey prey n/a 867383 unspecified role unspecified role prey prey n/a 867384 unspecified role unspecified role prey prey n/a 867385 unspecified role unspecified role prey prey n/a 867386 unspecified role unspecified role prey prey n/a 867387 unspecified role unspecified role prey prey physical association physical association false false false Fig. 3 and Table 2. cag3_helpy-1 867374 n/a 867378 unspecified role unspecified role bait bait n/a 867381 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 3B cag3_helpy-2 867375 n/a 867381 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 867378 unspecified role unspecified role prey prey physical association physical association false false false Fig. 3B