IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ lin-2009-1 Phosphorylation of SOS3-LIKE CALCIUM BINDING PROTEIN8 by SOS2 Protein Kinase Stabilizes Their Protein Complex and Regulates Salt Tolerance in Arabidopsis. in vitro In vitro protein kinase assay protein kinase assay autoradiography autoradiography Lin H., Yang Y., Quan R., Mendoza I., Wu Y., Du W., Zhao S., Schumaker KS., Pardo JM., Guo Y. The source of the MBP protein used in the study is not given , therefore interactions involving MBP protein were not curated. guoyan@nibs.ac.cn. Antibodies for the Ser 237 phosphorylation site within the SCaBP8 protein. Plant Cell (1040-4651) 2009 Only protein-protein interactions imex curation lin-2009-2 Phosphorylation of SOS3-LIKE CALCIUM BINDING PROTEIN8 by SOS2 Protein Kinase Stabilizes Their Protein Complex and Regulates Salt Tolerance in Arabidopsis. in vitro In vitro protein kinase assay protein kinase assay western blot western blot Immuno blot Immuno blot Lin H., Yang Y., Quan R., Mendoza I., Wu Y., Du W., Zhao S., Schumaker KS., Pardo JM., Guo Y. The source of the MBP protein used in the study is not given , therefore interactions involving MBP protein were not curated. guoyan@nibs.ac.cn. Antibodies for the Ser 237 phosphorylation site within the SCaBP8 protein. Plant Cell (1040-4651) 2009 Only protein-protein interactions imex curation lin-2009-3 Phosphorylation of SOS3-LIKE CALCIUM BINDING PROTEIN8 by SOS2 Protein Kinase Stabilizes Their Protein Complex and Regulates Salt Tolerance in Arabidopsis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lin H., Yang Y., Quan R., Mendoza I., Wu Y., Du W., Zhao S., Schumaker KS., Pardo JM., Guo Y. The source of the MBP protein used in the study is not given , therefore interactions involving MBP protein were not curated. guoyan@nibs.ac.cn. Antibodies for the Ser 237 phosphorylation site within the SCaBP8 protein. Plant Cell (1040-4651) 2009 Only protein-protein interactions imex curation lin-2009-4 Phosphorylation of SOS3-LIKE CALCIUM BINDING PROTEIN8 by SOS2 Protein Kinase Stabilizes Their Protein Complex and Regulates Salt Tolerance in Arabidopsis. arath-mesophyll Arabidopsis thaliana mesophyll mesophyll Mesophyll of plant leaf The photosynthetic parenchyma between the epidermal layers of a foliage leaf. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Lin H., Yang Y., Quan R., Mendoza I., Wu Y., Du W., Zhao S., Schumaker KS., Pardo JM., Guo Y. The source of the MBP protein used in the study is not given , therefore interactions involving MBP protein were not curated. Mesophyll protoplasts were prepared and transfected using Arabidopsis leaves as described in Sheen, 2001. guoyan@nibs.ac.cn. Antibodies for the Ser 237 phosphorylation site within the SCaBP8 protein. Plant Cell (1040-4651) 2009 Only protein-protein interactions imex curation cipko_arath CBL-interacting serine/threonine-protein kinase 24 SNF1-related kinase 3.11 Protein SALT OVERLY SENSITIVE 2 CIPK24 SnRK3.11 SOS2 At5g35410 F6I13.1 K21B8.3 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTKKMRRVGKYEVGRTIGEGTFAKVKFARNTDTGDNVAIKIMAKSTILKNRMVDQIKREISIMKIVRHPNIVRLYEVLASPSKIYIVLEFVTGGELFDRIVHKGRLEESESRKYFQQLVDAVAHCHCKGVYHRDLKPENLLLDTNGNLKVSDFGLSALPQEGVELLRTTCGTPNYVAPEVLSGQGYDGSAADIWSCGVILFVILAGYLPFSETDLPGLYRKINAAEFSCPPWFSAEVKFLIHRILDPNPKTRIQIQGIKKDPWFRLNYVPIRAREEEEVNLDDIRAVFDGIEGSYVAENVERNDEGPLMMNAFEMITLSQGLNLSALFDRRQDFVKRQTRFVSRREPSEIIANIEAVANSMGFKSHTRNFKTRLEGLSSIKAGQLAVVIEIYEVAPSLFMVDVRKAAGETLEYHKFYKKLCSKLENIIWRATEGIPKSEILRTITF q7frs8-2 Calcineurin B-like protein 10 SOS3-like calcium binding protein 8 F26P21_120 At4g33000 CBL10 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEQVSSRSSSLTVGEQFCAVFIPFFAIIDVLVSSVGQCFDCRSTSPRTCQHADLERLARESQFSVNEVEALYELFKKLSCSIIDDGLIHKEELRLALFQAPYGENLFLDRVFDLFDEKKNGVIEFEEFIHALSVFHPYASIQEKTDFAFRLYDLRQTGFIEREEVQQMVSAILLESDMMLSDELLTMIIDKTFADADSDKDGKISKDEWNVYVHKHPSLLKNMTLPYLKDVTTAFPSFIFNTEVED May be due to a competing donor splice site. sos2-scabp8 830403 n/a 830407 enzyme enzyme neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 830408 enzyme target enzyme target substrate substrate neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false c-terminal sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q7FRS8-2:p.Ser237Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false Figure 1 Figure 2 Phosphorylation of SCaBP8 at the Ser 237 position. The presence of the SOS2 protein FISL domain (INTERPRO identifier, IPR018451) , as shown by deletion and addition of a domian specific peptide to phosphorylation assays, significantly reduced phosphorrylation of SCaBP8. sos2 830403 n/a 830407 putative self putative self putative self putative self region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false true false Figure 1 Figure 2 Autophosphorylation sos2-scabp8-1 830404 n/a 830407 enzyme enzyme neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 830408 enzyme target enzyme target substrate substrate neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false Figure 3A Phosphorylation of SCaBP8 at the Ser 237 position sos2-scabp8-2 830405 n/a 830408 unspecified role unspecified role bait bait Q7FRS8-2:p.Ser237Asp mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q7FRS8-2:p.Ser237Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false n/a 830407 unspecified role unspecified role prey prey physical association physical association false false false Figure 6A sos2-scabp8-3 830406 n/a 830408 unspecified role unspecified role prey prey Q7FRS8-2:p.Ser237Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q7FRS8-2:p.Ser237Asp mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false n/a 830407 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Figure 6B