IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ walia-2009-1 Arabidopsis Mitogen-Activated Protein Kinase MPK18 Mediates Cortical Microtubule Functions in Plant Cells. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Walia A., Lee JS., Wasteneys G., Ellis B. bee@interchange.ubc.ca Plant J. (0960-7412) 2009 Only protein-protein interactions imex curation walia-2009-2 Arabidopsis Mitogen-Activated Protein Kinase MPK18 Mediates Cortical Microtubule Functions in Plant Cells. nicbe Nicotiana benthamiana bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence Walia A., Lee JS., Wasteneys G., Ellis B. bee@interchange.ubc.ca Plant J. (0960-7412) 2009 Only protein-protein interactions imex curation walia-2009-3 Arabidopsis Mitogen-Activated Protein Kinase MPK18 Mediates Cortical Microtubule Functions in Plant Cells. in vitro In vitro phosphatase assay phosphatase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Walia A., Lee JS., Wasteneys G., Ellis B. bee@interchange.ubc.ca Plant J. (0960-7412) 2009 Only protein-protein interactions imex curation mpk12_arath Mitogen-activated protein kinase 12 MPK12 T3F17.28 At2g46070 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSGESSSGSTEHCIKVVPTHGGRYVQYNVYGQLFEVSRKYVPPIRPIGRGACGIVCAAVNSVTGEKVAIKKIGNAFDNIIDAKRTLREIKLLRHMDHENVITIKDIVRPPQRDIFNDVYIVYELMDTDLQRILRSNQTLTSDQCRFLVYQLLRGLKYVHSANILHRDLRPSNVLLNSKNELKIGDFGLARTTSDTDFMTEYVVTRWYRAPELLLNCSEYTAAIDIWSVGCILGEIMTGQPLFPGKDYVHQLRLITELVGSPDNSSLGFLRSDNARRYVRQLPRYPKQQFAARFPKMPTTAIDLLERMLVFDPNRRISVDEALGHAYLSPHHDVAKEPVCSTPFSFDFEHPSCTEEHIKELIYKESVKFNPDH dphs1_arath Dual specificity protein phosphatase PHS1 PHS1 Protein PROPYZAMIDE-HYPERSENSITIVE 1 MQM1.1 At5g23720 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAEPEKKRDQPFSQEKDEEKDLYLVHDEHESPLPLTVTSRVLYMLGDIASGPAYRFTQWLDLVRKRSATYGSSGFPHRLHRIDDMVTSAGERNTDPKSPPSRQSSEISLWERLGKASTVDIDSSCFSWNMLSSLHHTEHSSSTDHSEEDQSKPLEVTVNSGGVVFFALFNSSSSEDASRKEEAAVIKFASSRMATQSERLGYEFSKWLGVQIPQARVIHSCNPEWTLIKEATEKAQAKATSEGDEVGEMTCSELLEALELSRCLLLMSYVHGCPMLESMSSFETEEKAERAAAALGRILVLDLVIRNEDRLPCRQLRWRGNPANLLLTDRIVSSAKHHECSFDEAFDSAIKRYHPKDYRSIQRERRASSVDSRSRLSISDQMLVSQASDFSDITESPRSYDTGLMSPMSDRSVAADFHLVAIDSGVPRRPPAGKRASDQEIYPRLVELLLNSSQYSSNLLHEITEGSLGYPQAEDGEETSNVRSVVTPVVREFRNGFRAGLRDLQEFHIFLVTLHQKLDVLLRAFFSMMDKTMCADFDREDFAVPESPSHTHGHEVNHYPSPSKDRVPSDNSSDHSESDMQKSVPRTPNSENKEDGSSPKSRESWHGRSGKGGESLSSQRLAAKLRDFHKFAKVDAESNKELDQWNETLRNEVMKLCQENGFNTGFFEGSDNNSCTDAYELKVRLEHILERISLISKAANTEKPSMIQENLFIGGGLAARSIYTLQHLGITHVLCLCANEIGQSDTQYPDLFEYQNFSITDDEDSNIESIFQEALDFIKHGEETGGKILVHCFEGRSRSATVVLAYLMLQKKLTLLEAWSKLRKVHRRAQPNDGFARILINLDKKCHGKVSMEWRQRKPTMKVCPVCGKNAGLSSSSLKLHLQKSHRKLSSGSVDSAMNMEIQKALEALKLSTGRGSSASSNSFQSHPG mpk18_arath Mitogen-activated protein kinase 18 MPK18 At1g53510 F22G10.12 T3F20.17 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MQQNQVKKGTKEMEFFTEYGDANRYRILEVIGKGSYGVVCAAIDTHTGEKVAIKKINDVFEHISDALRILREVKLLRLLRHPDIVEIKSIMLPPSKREFKDIYVVFELMESDLHQVIKANDDLTREHHQFFLYQMLRALKFMHTANVYHRDLKPKNILANANCKLKVCDFGLARVAFNDTPTTVFWTDYVATRWYRAPELCGSFFSKYTPAIDVWSIGCIFAEVLTGKPLFPGKSVVHQLELITDLLGTPKSETISGVRNDKARKYLTEMRKKNPVTFSQKFSKADPLALRLLQRLLAFDPKDRPTPAEALADPYFKGLSKIEREPSSQQISKMEFEFERRRLTKDDIRELIYREILEYHPQLLKDYMSGSEGSNFVYPSAIGHLRQQFTYLEENSSRNGPVIPLERKHASLPRSTVHSTVVHSTSQPNLGATDSRRVSFEPSKNGASSAGHPSTSAYPTKSIGPPPRVPPSGRPGRVVESSVSYENGRNLKEAYFRSAVSSPHCYFRPNTMTNPENRNIEASSFPPKPQNPVHQFSPTEPPAATTNQADVETMNHPNPYFQPQLPKTDQLNNNTHMAIDAKLLQAQSQFGPAGAAAVAVAAHRNIGTISYSAAS phs1-mpk12 824479 n/a 824482 unspecified role unspecified role prey prey n/a 824483 unspecified role unspecified role bait bait physical association physical association false false false Figure 1a phs1-mpk18 824479 n/a 824483 unspecified role unspecified role bait bait n/a 824484 unspecified role unspecified role prey prey physical association physical association false false false Figure 1a phs1-mpk18-1 824480 n/a 824484 unspecified role unspecified role neutral component neutral component region N-terminal region of YFP protein yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 824483 unspecified role unspecified role neutral component neutral component region C-terminal region of YFP protein yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false Figure 1b phs1-mpk18-2 824481 n/a 824483 enzyme enzyme neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 824484 enzyme target enzyme target substrate substrate neutral component neutral component residue phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) dephosphorylation dephosphorylation reaction dephosphorylation false false false Figure 1c MPK18 was phosphorylated by autophosphorylation