IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ creppe-2009-1 Elongator controls the migration and differentiation of cortical neurons through acetylation of alpha-tubulin. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Creppe C., Malinouskaya L., Volvert ML., Gillard M., Close P., Malaise O., Laguesse S., Cornez I., Rahmouni S., Ormenese S., Belachew S., Malgrange B., Chapelle JP., Siebenlist U., Moonen G., Chariot A., Nguyen L. Data pertaining to alpha-tubulin could not be curated as this could not be associated with a single gene product. alain.chariot@ulg.ac.be, lnguyen@ulg.ac.be Cell (0092-8674) 2009 Only protein-protein interactions 4 imex curation creppe-2009-2 Elongator controls the migration and differentiation of cortical neurons through acetylation of alpha-tubulin. human-sk_n_be(2) Human caucasian neuroblastoma cell line sk_n_be(2) Human neuroblastoma cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Creppe C., Malinouskaya L., Volvert ML., Gillard M., Close P., Malaise O., Laguesse S., Cornez I., Rahmouni S., Ormenese S., Belachew S., Malgrange B., Chapelle JP., Siebenlist U., Moonen G., Chariot A., Nguyen L. Data pertaining to alpha-tubulin could not be curated as this could not be associated with a single gene product. alain.chariot@ulg.ac.be, lnguyen@ulg.ac.be Cell (0092-8674) 2009 Only protein-protein interactions 4 imex curation creppe-2009-3 Elongator controls the migration and differentiation of cortical neurons through acetylation of alpha-tubulin. human-ht_29 Homo sapiens HT-29 caucasian women with colon adenocarcinoma ht_29 HT-29 confirmed as human with IEF of AST, LDH established from the primary tumor of a 44-year-old Caucasian woman with colon adenocarcinoma in 1964; described to be heterotransplantable forming well-differentiated grade I tumors anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Creppe C., Malinouskaya L., Volvert ML., Gillard M., Close P., Malaise O., Laguesse S., Cornez I., Rahmouni S., Ormenese S., Belachew S., Malgrange B., Chapelle JP., Siebenlist U., Moonen G., Chariot A., Nguyen L. Data pertaining to alpha-tubulin could not be curated as this could not be associated with a single gene product. alain.chariot@ulg.ac.be, lnguyen@ulg.ac.be Cell (0092-8674) 2009 Only protein-protein interactions 4 imex curation creppe-2009-4 Elongator controls the migration and differentiation of cortical neurons through acetylation of alpha-tubulin. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Creppe C., Malinouskaya L., Volvert ML., Gillard M., Close P., Malaise O., Laguesse S., Cornez I., Rahmouni S., Ormenese S., Belachew S., Malgrange B., Chapelle JP., Siebenlist U., Moonen G., Chariot A., Nguyen L. Data pertaining to alpha-tubulin could not be curated as this could not be associated with a single gene product. alain.chariot@ulg.ac.be, lnguyen@ulg.ac.be Cell (0092-8674) 2009 Only protein-protein interactions 4 imex curation elp3_human Elongator complex protein 3 ELP3 Protein lysine acetyltransferase ELP3 tRNA uridine(34) acetyltransferase protein protein human Homo sapiens Human MRQKRKGDLSPAELMMLTIGDVIKQLIEAHEQGKDIDLNKVKTKTAAKYGLSAQPRLVDIIAAVPPQYRKVLMPKLKAKPIRTASGIAVVAVMCKPHRCPHISFTGNICVYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYDPFLQTRHRIEQLKQLGHSVDKVEFIVMGGTFMALPEEYRDYFIRNLHDALSGHTSNNIYEAVKYSERSLTKCIGITIETRPDYCMKRHLSDMLTYGCTRLEIGVQSVYEDVARDTNRGHTVKAVCESFHLAKDSGFKVVAHMMPDLPNVGLERDIEQFTEFFENPAFRPDGLKLYPTLVIRGTGLYELWKSGRYKSYSPSDLVELVARILALVPPWTRVYRVQRDIPMPLVSSGVEHGNLRELALARMKDLGIQCRDVRTREVGIQEIHHKVRPYQVELVRRDYVANGGWETFLSYEDPDQDILIGLLRLRKCSEETFRFELGGGVSIVRELHVYGSVVPVSSRDPTKFQHQGFGMLLMEEAERIAREEHGSGKIAVISGVGTRNYYRKIGYRLQGPYMVKMLK elp1_human Elongator complex protein 1 ELP1 IKAP IKBKAP p150 IkappaB kinase complex-associated protein protein protein human Homo sapiens Human MRNLKLFRTLEFRDIQGPGNPQCFSLRTEQGTVLIGSEHGLIEVDPVSREVKNEVSLVAEGFLPEDGSGRIVGVQDLLDQESVCVATASGDVILCSLSTQQLECVGSVASGISVMSWSPDQELVLLATGQQTLIMMTKDFEPILEQQIHQDDFGESKFITVGWGRKETQFHGSEGRQAAFQMQMHESALPWDDHRPQVTWRGDGQFFAVSVVCPETGARKVRVWNREFALQSTSEPVAGLGPALAWKPSGSLIASTQDKPNQQDIVFFEKNGLLHGHFTLPFLKDEVKVNDLLWNADSSVLAVWLEDLQREESSIPKTCVQLWTVGNYHWYLKQSLSFSTCGKSKIVSLMWDPVTPYRLHVLCQGWHYLAYDWHWTTDRSVGDNSSDLSNVAVIDGNRVLVTVFRQTVVPPPMCTYQLLFPHPVNQVTFLAHPQKSNDLAVLDASNQISVYKCGDCPSADPTVKLGAVGGSGFKVCLRTPHLEKRYKIQFENNEDQDVNPLKLGLLTWIEEDVFLAVSHSEFSPRSVIHHLTAASSEMDEEHGQLNVSSSAAVDGVIISLCCNSKTKSVVLQLADGQIFKYLWESPSLAIKPWKNSGGFPVRFPYPCTQTELAMIGEEECVLGLTDRCRFFINDIEVASNITSFAVYDEFLLLTTHSHTCQCFCLRDASFKTLQAGLSSNHVSHGEVLRKVERGSRIVTVVPQDTKLVLQMPRGNLEVVHHRALVLAQIRKWLDKLMFKEAFECMRKLRINLNLIYDHNPKVFLGNVETFIKQIDSVNHINLFFTELKEEDVTKTMYPAPVTSSVYLSRDPDGNKIDLVCDAMRAVMESINPHKYCLSILTSHVKKTTPELEIVLQKVHELQGNAPSDPDAVSAEEALKYLLHLVDVNELYDHSLGTYDFDLVLMVAEKSQKDPKEYLPFLNTLKKMETNYQRFTIDKYLKRYEKAIGHLSKCGPEYFPECLNLIKDKNLYNEALKLYSPSSQQYQDISIAYGEHLMQEHMYEPAGLMFARCGAHEKALSAFLTCGNWKQALCVAAQLNFTKDQLVGLGRTLAGKLVEQRKHIDAAMVLEECAQDYEEAVLLLLEGAAWEEALRLVYKYNRLDIIETNVKPSILEAQKNYMAFLDSQTATFSRHKKRLLVVRELKEQAQQAGLDDEVPHGQESDLFSETSSVVSGSEMSGKYSHSNSRISARSSKNRRKAERKKHSLKEGSPLEDLALLEALSEVVQNTENLKDEVYHILKVLFLFEFDEQGRELQKAFEDTLQLMERSLPEIWTLTYQQNSATPVLGPNSTANSIMASYQQQKTSVPVLDAELFIPPKINRRTQWKLSLLD elp1-elp3-1 Interaction between ELP1 and ELP3 determined by coimmunoprecipitation 801507 n/a 801511 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 801512 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 4B elp1-elp3-2 Interaction between ELP1 and ELP3 determined by coimmunoprecipitation 801508 n/a 801512 unspecified role unspecified role prey prey n/a 801511 unspecified role unspecified role bait bait physical association physical association false false false Supp. Fig. 10 elp1-elp3-3 Interaction between ELP1 and ELP3 determined by coimmunoprecipitation 801509 n/a 801511 unspecified role unspecified role bait bait n/a 801512 unspecified role unspecified role prey prey physical association physical association false false false Supp. Fig. 10 elp1-elp3-4 Interaction between ELP1 and ELP3 determined by coimmunoprecipitation 801510 n/a 801511 unspecified role unspecified role bait bait n/a 801512 unspecified role unspecified role prey prey physical association physical association false false false Supp. Fig. 10