IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ fujimoto-2009-1 Arabidopsis dynamin-related proteins DRP3A and DRP3B are functionally redundant in mitochondrial fission but have distinct roles in peroxisomal fission. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Fujimoto M., Arimura SI., Mano S., Kondo M., Saito C., Ueda T., Nakazono M., Nakano A., Nishimura M., Tsutsumi N. atsutsu@mail.ecc.u-tokyo.ac.jp Plant J. (0960-7412) 2009 Only protein-protein interactions imex curation fujimoto-2009-3 Arabidopsis dynamin-related proteins DRP3A and DRP3B are functionally redundant in mitochondrial fission but have distinct roles in peroxisomal fission. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Fujimoto M., Arimura SI., Mano S., Kondo M., Saito C., Ueda T., Nakazono M., Nakano A., Nishimura M., Tsutsumi N. atsutsu@mail.ecc.u-tokyo.ac.jp Plant J. (0960-7412) 2009 Only protein-protein interactions imex curation fujimoto-2009-2 Arabidopsis dynamin-related proteins DRP3A and DRP3B are functionally redundant in mitochondrial fission but have distinct roles in peroxisomal fission. arath-leaf Arabidopsis thaliana leaf leaf leaf fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Fujimoto M., Arimura SI., Mano S., Kondo M., Saito C., Ueda T., Nakazono M., Nakano A., Nishimura M., Tsutsumi N. Plasmids for visualizing mitochondria and peroxisomes were introduced into leaf epidermal cells via particle bombardment, by using a helium-driven particle accelerator (PDS/1000; Bio-Rad, http://www.bio-rad.com), with all basic adjustments set according to the manufacturer�s recommendations. atsutsu@mail.ecc.u-tokyo.ac.jp Plant J. (0960-7412) 2009 Only protein-protein interactions imex curation fujimoto-2009-4 Arabidopsis dynamin-related proteins DRP3A and DRP3B are functionally redundant in mitochondrial fission but have distinct roles in peroxisomal fission. arath-leaf Arabidopsis thaliana leaf leaf leaf confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Fujimoto M., Arimura SI., Mano S., Kondo M., Saito C., Ueda T., Nakazono M., Nakano A., Nishimura M., Tsutsumi N. Tagged proteins were expressed in transgenic Arabidopsis. atsutsu@mail.ecc.u-tokyo.ac.jp Plant J. (0960-7412) 2009 Only protein-protein interactions imex curation drp3a_arath Dynamin-related protein 3A DRP3A ADL2 ADL2A Dynamin-like protein 2a Dynamin-like protein 2 T16L1.140 At4g33650 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTIEEVSGETPPSTPPSSSTPSPSSSTTNAAPLGSSVIPIVNKLQDIFAQLGSQSTIALPQVVVVGSQSSGKSSVLEALVGRDFLPRGNDICTRRPLVLQLLQTKSRANGGSDDEWGEFRHLPETRFYDFSEIRREIEAETNRLVGENKGVADTQIRLKISSPNVLNITLVDLPGITKVPVGDQPSDIEARIRTMILSYIKQDTCLILAVTPANTDLANSDALQIASIVDPDGHRTIGVITKLDIMDKGTDARKLLLGNVVPLRLGYVGVVNRCQEDILLNRTVKEALLAEEKFFRSHPVYHGLADRLGVPQLAKKLNQILVQHIKVLLPDLKSRISNALVATAKEHQSYGELTESRAGQGALLLNFLSKYCEAYSSLLEGKSEEMSTSELSGGARIHYIFQSIFVKSLEEVDPCEDLTDDDIRTAIQNATGPRSALFVPDVPFEVLVRRQISRLLDPSLQCARFIFEELIKISHRCMMNELQRFPVLRKRMDEVIGDFLREGLEPSEAMIGDIIDMEMDYINTSHPNFIGGTKAVEAAMHQVKSSRIPHPVARPKDTVEPDRTSSSTSQVKSRSFLGRQANGIVTDQGVVSADAEKAQPAANASDTRWGIPSIFRGGDTRAVTKDSLLNKPFSEAVEDMSHNLSMIYLKEPPAVLRPTETHSEQEAVEIQITKLLLRSYYDIVRKNIEDSVPKAIMHFLVNHTKRELHNVFIKKLYRENLFEEMLQEPDEIAVKRKRTQETLHVLQQAYRTLDELPLEADSVSAGMSKHQELLTSSKYSTSSSYSASPSTTRRSRRAGDQHQNGYGF drp3b_arath Dynamin-related protein 3B DRP3B ADL2B Dynamin-like protein 2b T22C12.1 At2g14120 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSVDDLPPSSASAVTPLGSSVIPIVNKLQDIFAQLGSQSTIALPQVAVVGSQSSGKSSVLEALVGRDFLPRGNDICTRRPLRLQLVQTKPSSDGGSDEEWGEFLHHDPVRRIYDFSEIRREIEAETNRVSGENKGVSDIPIGLKIFSPNVLDISLVDLPGITKVPVGDQPSDIEARIRTMILTYIKEPSCLILAVSPANTDLANSDALQIAGNADPDGHRTIGVITKLDIMDRGTDARNHLLGKTIPLRLGYVGVVNRSQEDILMNRSIKDALVAEEKFFRSRPVYSGLTDRLGVPQLAKKLNQVLVQHIKALLPSLKSRINNALFATAKEYESYGDITESRGGQGALLLSFITKYCEAYSSTLEGKSKEMSTSELSGGARILYIFQSVFVKSLEEVDPCEDLTADDIRTAIQNATGPRSALFVPDVPFEVLVRRQISRLLDPSLQCARFIFDELVKISHQCMMKELQRFPVLQKRMDEVIGNFLREGLEPSQAMIRDLIEMEMDYINTSHPNFIGGTKAVEQAMQTVKSSRIPHPVARPRDTVEPERTASSGSQIKTRSFLGRQANGIITDQAVPTAADAERPAPAGSTSWSGFSSIFRGSDGQAAAKNNLLNKPFSETTQEVYQNLSTIYLKEPPTILKSSETHSEQESVEIEITKLLLKSYYDIVRKNVEDLVPKAIMHFLVNYTKRELHNVFIEKLYRENLIEELLKEPDELAIKRKRTQETLRILQQANRTLDELPLEAESVERGYKIGSEAKHEELPGTRRSRTETNGNGRLHM drp1a_arath Phragmoplastin DRP1A DRP1A ADL1 ADL1A AG68 Dynamin-like protein 1 Protein RADIAL SWELLING 9 RSW9 MJC20.19 At5g42080 Dynamin-like protein 1A Dynamin-related protein 1A protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MENLISLVNKIQRACTALGDHGDSSALPTLWDSLPAIAVVGGQSSGKSSVLESIVGKDFLPRGSGIVTRRPLVLQLQKIDDGTREYAEFLHLPRKKFTDFAAVRKEIQDETDRETGRSKAISSVPIHLSIYSPNVVNLTLIDLPGLTKVAVDGQSDSIVKDIENMVRSYIEKPNCIILAISPANQDLATSDAIKISREVDPSGDRTFGVLTKIDLMDKGTDAVEILEGRSFKLKYPWVGVVNRSQADINKNVDMIAARKREREYFSNTTEYRHLANKMGSEHLAKMLSKHLERVIKSRIPGIQSLINKTVLELETELSRLGKPIAADAGGKLYSIMEICRLFDQIFKEHLDGVRAGGEKVYNVFDNQLPAALKRLQFDKQLAMDNIRKLVTEADGYQPHLIAPEQGYRRLIESSIVSIRGPAEASVDTVHAILKDLVHKSVNETVELKQYPALRVEVTNAAIESLDKMREGSKKATLQLVDMECSYLTVDFFRKLPQDVEKGGNPTHSIFDRYNDSYLRRIGSNVLSYVNMVCAGLRNSIPKSIVYCQVREAKRSLLDHFFAELGTMDMKRLSSLLNEDPAIMERRSAISKRLELYRAAQSEIDAVAWSK drp3a DRP3A homodimer 798496 n/a 798500 unspecified role unspecified role bait bait physical association physical association false false false Figure 1e drp3a-drp3b DRP3A interacts with DRP3B 798496 n/a 798501 unspecified role unspecified role prey prey n/a 798500 unspecified role unspecified role bait bait physical association physical association false false false Figure 1e drp3b-drp3a DRP3AB interacts with DRP3A 798496 n/a 798501 unspecified role unspecified role bait bait n/a 798500 unspecified role unspecified role prey prey physical association physical association false false false Figure 1e drp3b-1 DRP3AB homodimer 798496 n/a 798501 unspecified role unspecified role bait bait n/a 798501 unspecified role unspecified role prey prey physical association physical association false false false Figure 1e drpa1 DRPA1 homodimer 798496 n/a 798502 unspecified role unspecified role bait bait n/a 798502 unspecified role unspecified role prey prey physical association physical association false false false Figure 1e drp3a-1 DRP3A homodimer 798497 n/a 798500 unspecified role unspecified role prey prey n/a 798500 unspecified role unspecified role bait bait physical association physical association false false false Figure 1b drp3a-drp3b-1 DRP3A interacts with DRP3B 798497 n/a 798500 unspecified role unspecified role bait bait n/a 798501 unspecified role unspecified role prey prey physical association physical association false false false Figure 1b drp3b-drp3a-2 DRP3B interacts with DRP3A 798497 n/a 798500 unspecified role unspecified role prey prey n/a 798501 unspecified role unspecified role bait bait physical association physical association false false false Figure 1b drp3b-3 DRP3B homodimer 798497 n/a 798501 unspecified role unspecified role prey prey physical association physical association false false false Figure 1b drp3b-drp3a-1 DRP3AB colocalizes with DRP3A in Arabidopsis epidermal cells 798498 n/a 798501 unspecified role unspecified role neutral component neutral component c-terminal monomeric Kusabira Orange (mKO) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 798500 unspecified role unspecified role neutral component neutral component c-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false colocalization colocalization false false false Figure 2a DRP3AB colocalizes with DRP3A in Arabidopsis epidermal cells drp3b-drp3a-3 DRP3B colocalises DRP3A 798499 n/a 798501 unspecified role unspecified role neutral component neutral component c-terminal Kusabira Orange tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 798500 unspecified role unspecified role prey prey c-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false colocalization colocalization false false false Figure 2a The authors state that "confocal laser scanning microscopic (CLSM) images of leaf epidermal cells, both the DRP3A:GFP and DRP3B:mKO signals were dot-like (Figure 2a). In almost all (97.8 1.3%, mean SD) of the dot-like signals of Figure 2a (based on 10 CLSM images of a 48:48 lm epidermal cell area), the DRP3A:GFP and DRP3B:mKO signals overlapped".