IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ zhao-2009-3 Interaction between Arabidopsis Ca2+/H+ exchangers CAX1 and CAX3. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast ub reconstruction ubiquitin reconstruction ub reconstruction predetermined predetermined participant predetermined Zhao J., Shigaki T., Mei H., Guo YQ., Cheng NH., Hirschi KD. Transgenic Arabidopsis (ecotype Columbia) expressing epitope tagged proteins were used in these studies. Microsomal preparations were obtained from transgenic plant leaves. kendalh@bcm.edu J. Biol. Chem. (0021-9258) 2009 Only protein-protein interactions imex curation zhao-2009-1 Interaction between Arabidopsis Ca2+/H+ exchangers CAX1 and CAX3. arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Zhao J., Shigaki T., Mei H., Guo YQ., Cheng NH., Hirschi KD. Transgenic Arabidopsis (ecotype Columbia) expressing epitope tagged proteins were used in these studies. Microsomal preparations were obtained from transgenic plant leaves. kendalh@bcm.edu J. Biol. Chem. (0021-9258) 2009 Only protein-protein interactions imex curation zhao-2009-2 Interaction between Arabidopsis Ca2+/H+ exchangers CAX1 and CAX3. arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Zhao J., Shigaki T., Mei H., Guo YQ., Cheng NH., Hirschi KD. Transgenic Arabidopsis (ecotype Columbia) expressing epitope tagged proteins were used in these studies. Microsomal preparations were obtained from transgenic plant leaves. kendalh@bcm.edu J. Biol. Chem. (0021-9258) 2009 Only protein-protein interactions imex curation cax3_arath Vacuolar cation/proton exchanger 3 CAX3 Ca(2+)/H(+) exchanger 3 Ca(2+)/H(+) antiporter CAX3 Protein CATION EXCHANGER 3 ATEM1.11 At3g51860 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGSIVEPWAAIAENGNANVTAKGSSRELRHGRTAHNMSSSSLRKKSDLRLVQKVPCKTLKNILSNLQEVILGTKLTLLFLAIPLAILANSYNYGRPLIFGLSLIGLTPLAERVSFLTEQLAFYTGPTVGGLLNATCGNATELIIAILALANNKVAVVKYSLLGSILSNLLLVLGTSLFFGGIANIRREQRFDRKQADVNFFLLLMGLLCHLLPLLLKYAATGEVSTSMINKMSLTLSRTSSIVMLIAYIAYLIFQLWTHRQLFEAQQDDDDAYDDEVSVEETPVIGFWSGFAWLVGMTIVIALLSEYVVDTIEDASDSWGLSVSFISIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQISLFVVPLSVIVAWILGIKMDLNFNILETSSLALAIIITAFTLQDGTSHYMKGLVLLLCYVIIAACFFVDQIPQPNDLDVGLQPMNNLGEVFSA cax1_arath Vacuolar cation/proton exchanger 1 CAX1 RCI4 Ca(2+)/H(+) exchanger 1 Ca(2+)/H(+) antiporter CAX1 Protein RARE COLD INDUCIBLE 4 F16M14.10 At2g38170 Protein CATION EXCHANGER 1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAGIVTEPWSVAENGNPSITAKGSSRELRLGRTAHNMSSSSLRKKSDLRVIQKVPYKGLKDFLSNLQEVILGTKLAILFPAIPAAIICTYCGVSQPWIFGLSLLGLTPLAERVSFLTEQLAFYTGPTLGGLLNATCGNATELIIAILALTNNKVAVVKYSLLGSILSNLLLVLGTSLFCGGIANIRREQRFDRKQADVNFFLLLLGFLCHLLPLLVGYLKNGEASAAVLSDMQLSISRGFSIVMLISYIAYLVFQLWTHRQLFDAQEQEDEYDDDVEQETAVISFWSGFAWLVGMTLVIALLSEYVVATIEEASDKWNLSVSFISIILLPIVGNAAEHAGAVIFAFKNKLDISLGVALGSATQIGLFVVPLTIIVAWILGINMDLNFGPLETGCLAVSIIITAFTLQDGSSHYMKGLVLLLCYFIIAICFFVDKLPQKQNAIHLGHQAMNNVVTATGGGVFSS cax1-cax3-2 cax1 interacts with cax3 802139 n/a 802142 unspecified role unspecified role prey prey n/a 802143 unspecified role unspecified role bait bait physical association physical association false false false Figure 6C Figure 6D Accession numbers for CAX1 and CAX3 were obtained from references in the text. cax1-cax3-3 cax3 interacts with cax1 802139 n/a 802142 unspecified role unspecified role bait bait n/a 802143 unspecified role unspecified role prey prey physical association physical association false false false Figure 6C Figure 6D Accession numbers for CAX1 and CAX3 were obtained from references in the text. cax1-cax3 cax1 interacts with cax3 802140 n/a 802142 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 802143 unspecified role unspecified role bait bait physical association physical association false false false Figure 5A Figure 5D Accession numbers for CAX1 and CAX3 were obtained from references in the text. Lithium Chloride (15mM) treatment for 8 hours. cax1-cax3-1 cax1 interacts with cax3 802141 n/a 802143 unspecified role unspecified role prey prey n/a 802142 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Figure 5B Accession numbers for CAX1 and CAX3 were obtained from references in the text.